维普期刊资源整合服务平台（V6.5）

30篇 您的检索式：作者名="Poat"

选中清除导出查看参考文献查看引证文献引用追踪共2页首页上一页第1页下一页末页 /2 跳转

	题名	作者	年代	出处	被引量
1	Hepatocellular carcinoma xenograft supports HCV replication:A mouse model for evaluating antivirals显示文摘AIM: To develop a hepatocellular carcinoma (HCC) xenograft model for studying hepatitis C virus (HCV) replication in a mice, and antiviral treatment.METHODS: We developed a stable S3-green fluorescence protein (GFP) cell line that replicated the GFP-tagged HCV sub-genomic RNA derived from a highly efficient JFH1 virus. S3-GFP replicon cell line was injected subcutaneously into γ-irradiated SCID mice. We showed that the S3-GFP replicon cell line formed human HCC xenografts in SCID mice. Cells were isolated from subcutaneous tumors and then serially passaged multiple times in SCID mice by culturing in growth medium supplemented with G-418. The mouse-adapted S3-GFP replicon cells were implanted subcutaneously and also into the liver of SCID mice via intrasplenic infusion to study the replication of HCV in the HCC xenografts. The tumor model was validated for antiviral testing after intraperitoneal injection of interferon-α (IFN-α). RESULTS: A highly tumorigenic S3-GFP replicon cell line was developed that formed subcutaneous tumors within 2 wk and diffuse liver metastasis within 4 wk in SCID mice. Replication of HCV in the subcutaneous and liver tumors was confirmed by cell colony assay, detection of the viral RNA by ribonuclease protection assay and real-time quantitative reverse transcription polymerase chain reaction. High-level replication of HCV sub-genomic RNA in the tumor could be visualized by GFP expression using fluorescence microscopy. IFN-α cleared HCV RNA replication in the subcutaneous tumors within 2 wk and 4 wk in the liver tumor model. CONCLUSION: A non-infectious mouse model allows us to study replication of HCV in subcutaneous and metastatic liver tumors. Clearance of HCV by IFN-α supports use of this model to test other anti-HCV drugs.	Sidhartha Hazari Henry J Hefler Partha K Chandra Bret Poat Feyza Gunduz Tara Ooms Tong Wu Luis A Balart Srikanta Dash	2011	World Journal of Gastroenterology2011，17，3：	2
2	Electrical transport properties of CoSi2 and NiSi2 thin films 显示文摘	HENSEL J C TUNG R T POATE J M	1984	Appl Plays Let-t1984，44，9：	1
3	Electrical transport properties of CoSi2 and NiSi2 thin films 显示文摘	HENSEL J C TUNG RT POATE J M	1984	Appl Phys Lett1984，44，9：	1
4	Behcet's disease:a review显示文摘	PORTER SR POATE TW	2005	J Dent Res2005，84，3：	1
5	Beheet's disease a review显示文摘	AL - Otaibi LM Porter SR Poate TE	2005	J Dent Res2005，84，3：	1
6	Phys Rev Lett显示文摘	Tung R T Gibson J M Poate J M	1983	50: 4291983，50，：	1
7	Beh(c)et's disease:a review显示文摘	AL-OTAIBI L M PORTERSR POATE T W		0，，03：	1
8	Gettering Rates of Various Fast-Diffusing Metal Impurities at Ion Damagedc Layers on Silicon显示文摘	Pickar K A Poate J M	1972	Appl Phys Lett1972，21，10：	1
9	Diffusion mechanisms in the Pd/Au thin film system and the correla-tion of resistivity changes with Auger electron spectroscopy and Rutherford backscattering profiles 显示文摘	Hall P M Morabito J M Poate J M	1976	Thin Solids Films1976，33，1：	1
10	Differences between high affinity forskolin binding sites in dopamine rich and other regiaons of rat brain显示文摘	Poat JA Cripps HE Iversen LL	1988	Proc Nat 1 Acad Sci USA1988，85，9：	1
11	Diffusion mechanisms in the Pd/Au thin film system and the correlation of resistivity changes with Auger electron spectroscopy and Rutherford backscattering profiles显示文摘	Hall P M Morabito J M Poate J M	1976	Thin Solids Films1976，33，1：	1
12	Behcet's Disease: A Review显示文摘	Al-Otaibi L M Porter S R Poate T W	2005	J Dent Res2005，84，3：	1
13	SH2 modified STAT1 induces HLA-I expression and improves IFN- signaling in IFN-c resistant HCV replicon cells 显示文摘	POAT B HAZAR1 S CHANDRA PK	2010	PLoS One2010，5，9：	1
14	Thin Films Interdiffu- sion and Reactions 显示文摘	POATE J M TU K N MAYER J W	1978	Journal of Polymer Science: Poly- mer Letters Edition1978，17，1：	1
15	Gettering of silicon damager generated by ion implantation through SiO2 layer 显示文摘	Buck T M Pickar K A Poate C M	1972	Appl Phys Lett1972，21，10：	1
16	Insights into the Management Of Emerging Infections; Regulating Variant Creutzfeldt-Jakob Disease Transfusion Risk in the UK and the US显示文摘	Poate ML	2006	PLos Medicine2006，3，10：	1
17	Diffusion mechanisms in the Pd/Au thin film system and the correlation of resistivity changes with Auger electron spectroscopy and Ruthertord backscattering profiles显示文摘	Hall P M Morabito J M Poate J M	1976	Thin Solids Films1976，33，1：	1
18	Surface modification and alloying by laser,ion and electron beams显示文摘	Poate J M	1986	Plenum1986，3，：	1
19	Cyclic AMP efflux from rat striatal slices is enhanced by CCK显示文摘	O'Shaughnessy C Poat JA Turnbull MJ	1987	Biochem Pharmacol1987，36，：	1
20	显示文摘	Lanzerotti L J Brown W L Poate J M	1978	Nature1978，272，：	1

返回顶部每页显示：

共2页首页上一页第1页下一页末页 /2 跳转

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

用户提示