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| 1 | Identification of SUMO Targets by a Novel Proteomic Approach in Plants显示文摘Post-translational modifications (PTMs) chemically and physically alter the properties of proteins, including their folding, subcellular localization, stability, activity, and consequently their function. In spite of their relevance, studies on PTMs in plants are still limited. Small Ubiquitin-like Modifier (SUMO) modification regulates several biological processes by affecting protein-protein interactions, or changing the subcellular localizations of the target proteins. Here, we describe a novel proteomic approach to identify SUMO targets that combines 2-D liquid chromatography, immunodetection, and mass spectrometry (MS) analyses. We have applied this approach to identify nuclear SUMO targets in response to heat shock. Using a bacterial SUMOylation system, we validated that some of the targets identified here are, in fact, labeled with SUMO1. Interestingly, we found that GIGANTEA (GI), a photoperiodic-pathway protein, is modified with SUMO in response to heat shock both in vitro and in vivo. | Gema López-Torrejón Davide Guerra Rafael Catal Julio Salinas Juan C. del Pozo | 2013 | Journal of Integrative Plant Biology2013，55，1： | 6 |
| 2 | 日粮磷脂对大西洋鲑鱼早期发育和生长性能的影响显示文摘研究旨在证实大西洋鲑鱼对日粮磷脂的需求,更好地确定其需要水平和时期。因此,通过给大西洋鲑鱼提供磷虾或大豆卵磷脂探讨磷脂对其的影响。首先饲喂鱼苗含蛋白质55%和脂肪17%的饲粮,通过添加磷虾油或大豆磷脂补充脂肪.根据回归分析设计5个水平,分别为总磷脂1.5%(未添加)、2.6%、3.2%、3.6%和4.2%。在1g(受精后1400°dpf)、2.5g(1990°ape)、5g(2350°dpf)、10-20g(2850°dpf)和二龄鲑(3800°dpf)时进行取样。整体存活率高,说明存活率和饲粮磷脂添加水平呈正相关(r2=0.59—0.72)。磷脂促进了鱼苗的生长,且饲喂磷虾磷脂2.6%和大豆磷脂3.6%的鱼增长最快。鱼苗达到2.5g之前与之后生长模式不同,0-2.5g鱼补充磷虾磷脂2.6%和大豆磷脂3.6%后的生长速率(SGR)显著高于基础日粮(P〈0.05),而2.5g-二龄鲑期间,各处理之间没有差异。饲喂未补充磷脂的日粮,2.5g时发现鱼肠道脂肪变性,患病率达20%,饲喂低水平大豆磷脂日粮的鱼肠道脂肪变性患病率达10%,而喂食磷虾油和高水平大豆磷脂(≥3.2%)后以及在后期阶段,肠邋脂肪变性情况开始消失。在生命早期阶段,鲑鱼对日粮磷脂的要求是一致的。 | Taylor J F Martinez-Rubio L Pozo J D Walton J M Tinch A E Migaud H Tocher D R | 2015 | 饲料博览2015，0，8： | 2 |
| 3 | Detection of new respiratory viruses in hospitalized infants with bronchiolitis: a three-year r prospective study显示文摘 | Calvo C Pozo F Garcia-Garcia M L | 2010 | Acta Paediatrica2010，99，6： | 1 |
| 4 | A structural damage detection indicator based on principal component analysis and statistical hypothesis testing 显示文摘 | Mujica L E Ruiz M Pozo F | 2014 | Smart Materials and Structures2014，23，2： | 1 |
| 5 | Laser polishing of tool steel with CO 2 laser and high-power diode laser显示文摘 | E. Ukar A. Lamikiz L.N. López de Lacalle D. del Pozo J.L. Arana | 2009 | International Journal of Machine Tools and Manufacture2009，，1： | 1 |
| 6 | Fire Detection and Monitoring Using A Multitemporal Technique on AVHRR Mid-Infrared and Thermal Channels显示文摘 | Pozo D Olmo F J Alados-Arboledas L | 1997 | Remote Sensing of Environment1997，60，： | 1 |
| 7 | Inhibition of chronic airway inflammation and remodeling by galectin-3 gene therapy in a mu-rine model显示文摘 | L(o)pez E del Pozo V Miguel T | 2006 | J Immunol2006，176，3： | 1 |
| 8 | Coronatine and abscission in citrus显示文摘 | BURNS J K POZO L V ARIAS C R HOCKEMA B | 2003 | Journal of the American Society for Horticulture Science2003，128，3： | 1 |
| 9 | Application of mercury porosimetry to the study of xerogels used as stone consolidants显示文摘 | MOSQUERA M J POZO J ESQUIVIAS L | 2002 | Journal of Non -CrystaUine Solids2002，311，2： | 1 |
| 10 | The electricidal effect: reduction of Staphylococcus and Pseudomonas biofilms by prolonged exposure to low-intensity electrical current显示文摘 | del POZO J L ROUSE M S MANDREKAR J N | 2009 | Antimicrobial Agents and Chemotherapy2009，53，1： | 1 |
| 11 | Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana显示文摘 | GRAY W M DEL POZO J C WALKER L | 1999 | Genes Dev1999，13，： | 1 |
| 12 | Investigation of ultraviolet light-enhanced H202 oxidation of NOx emissions 显示文摘 | Cooper C D Clausen III C A Pettey L Collins M Pozo de Fernandez M | 2002 | Journal of Environmental Engineering2002，128，1： | 1 |
| 13 | Accumula- ted experience in the analysis of quality indicators in a haemo- dialysis unit显示文摘 | Del Pozo C Lopez- Menchero R Sdnchez L | 2009 | Nefrologia2009，29，1： | 1 |
| 14 | Coronal fractures of the lateral femoral condyle显示文摘 | LEWIS S L POZO J L MUIRHEAD-ALLWO()D W F | 1989 | Bone Joint Sur1989，71，1： | 1 |
| 15 | A new technique for the evaluation of a circulating fluidized bed mixing chamber with a central solids jet显示文摘 | MIRGAIN C BRIENS C L DEL POZO M | 1998 | Powder Technology1998，96，： | 1 |
| 16 | Mucosal and systemic isotype-specific antibody responses and protection in conventional pigs exposed to virulent or attenuated porcine epidemic diarrhoea virus显示文摘 | De Arriba M L Carvajal A Pozo J | 2002 | veterinary immunology and immunopathology2002，85，12： | 1 |
| 17 | Differential effects of 1-Methylcyclopropene on citrus leaf and mature fruit abscission显示文摘 | POZO L YUAN R C KOSTENYUK I FERNANDO A ZHONG G Y BURNS J K | 2004 | Physiol Plant2004，129，4： | 1 |
| 18 | Lymphopro- liferative responses and protection in conventional piglets inoculated orally with virulent or attenuated porcine epidemic diarrhoea virus显示文摘 | de Arriba M L Carvajal A Pozo J | 2002 | Journal of Viro- logical Methods2002，105，： | 1 |
| 19 | Effect of electri- cal current on the activities of antimicrobial agents against Pseudomonas aeruginoso, Staphylococcus attreus, and Stapfiylococcus epidermidis biofilms显示文摘 | DEL POZO J L ROUSE M S MANDREKAR J N | 2009 | Antimicrob Agents Ch2009，53，1： | 1 |
| 20 | High incidence of human bocavirus infection in children in Spain 显示文摘 | Pozo F Garcfa-Garcia M L Calvo C | 2007 | J Clin Virol2007，40，： | 1 |
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