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| 1 | A deep learning-enabled portable imaging flow cytometer for cost-effective, highthroughput, and label-free analysis of natural water samples显示文摘We report a deep learning-enabled field-portable and cost-effective imaging flow cytometer that automatically captures phase-contrast color images of the contents of a continuously flowing water sample at a throughput of 100 mL/h.The device is based on partially coherent lens-free holographic microscopy and acquires the diffraction patterns of flowing micro-objects inside a microfluidic channel.These holographic diffraction patterns are reconstructed in real time using a deep learning-based phase-recovery and image-reconstruction method to produce a color image of each micro-object without the use of external labeling.Motion blur is eliminated by simultaneously illuminating the sample with red,green,and blue light-emitting diodes that are pulsed.Operated by a laptop computer,this portable device measures 15.5 cm×15 cm×12.5 cm,weighs 1 kg,and compared to standard imaging flow cytometers,it provides extreme reductions of cost,size and weight while also providing a high volumetric throughput over a large object size range.We demonstrated the capabilities of this device by measuring ocean samples at the Los Angeles coastline and obtaining images of its micro-and nanoplankton composition.Furthermore,we measured the concentration of a potentially toxic alga(Pseudo-nitzschia)in six public beaches in Los Angeles and achieved good agreement with measurements conducted by the California Department of Public Health.The cost-effectiveness,compactness,and simplicity of this computational platform might lead to the creation of a network of imaging flow cytometers for largescale and continuous monitoring of the ocean microbiome,including its plankton composition. | Zoltán Gӧrӧcs Miu Tamamitsu Vittorio Bianco Patrick Wolf Shounak Roy Koyoshi Shindo Kyrollos Yanny Yichen Wu Hatice Ceylan Koydemir Yair Rivenson Aydogan Ozcan | 2018 | Light(Science & Applications)2018，7，1： | 14 |
| 2 | Maintenance infliximab for Crohn’s disease: the ACCENT I randomised trial显示文摘 | Stephen B Hanauer Brian G Feagan Gary R Lichtenstein Lloyd F Mayer S Schreiber Jean Frederic Colombel Daniel Rachmilewitz Douglas C Wolf Allan Olson Weihang Bao Paul Rutgeerts | 2002 | The Lancet2002，，9317： | 6 |
| 3 | Antecedents of organizational slack 显示文摘 | Sharman M P Wolf G Chase R B | 1988 | Academy of Management Review1988，13，4： | 1 |
| 4 | Erkl,2 Phosphorylates p27 (Kipl): functional evidence for a role in high glucose-induced hypertrophy of mesangial cells显示文摘 | Wolf G Reinking R Zahner G | 2003 | Diabetologia2003，46，： | 1 |
| 5 | Digital Image Fusion of Early and Delayed CT Scans:How to Achieve Optimal Opacification of Vessels and Squamous Cell Carcinomas of the Head and Neck显示文摘 | Groell R Wiltgen M Wolf G | 2002 | AJR2002，178，1： | 1 |
| 6 | Growth hormone receptor is a target for presenilin-dependent gam- ma-secretase cleavage 显示文摘 | Cowan J W Wang X D Guan R He K Jiang J Baumann G Black R A Wolfe M S Frank S J | 2005 | J Biol Chem2005，280，19： | 1 |
| 7 | Polo-like kinase:A novel marker of proliferation:Correlation with estrogen-receptor expression in human breast cancer 显示文摘 | Wolf G Hildenbrand R Schwar C | 2000 | Pathol Res Pract2000，196，11： | 1 |
| 8 | High glucose-inducedhypertrophy of mesangial cells requires p27,an inhibitor of cyclin-dependentkinases显示文摘 | Wolf G Schroeder R Zahner G | 2001 | Am J Pathol2001，158，： | 1 |
| 9 | North American Forest Disturbance Mapped from a Decadal Landsat Record显示文摘 | Masek J G Huang C Wolfe R | 2008 | Remote Sensing of Environment2008，112，6： | 1 |
| 10 | Estimating Within-group Interrater Reliability with and Without Response Bias显示文摘 | JAMES L R DEMAREE R G WOLF G | 1984 | Journal of Applied Psychology1984，69，1： | 1 |
| 11 | High Glucose-Induced Hypertrophy of Mesangial Cells Requires p27Kipl, an Inhibitor of Cyclin-Dependent Kinases显示文摘 | Wolf G Schroeder R Zahner G | 2001 | Am J Pathol2001，158，： | 1 |
| 12 | A prospective trial of ionic vs nonionic contrast agents in clinical practice:comparison of adverse effects显示文摘 | Arenson R L Cross A P | 1989 | AJR1989，152，： | 1 |
| 13 | Estimating Within-group Interrater Reliability with and without Response Bias显示文摘 | DEMAREE R G WOLF G | 1984 | Journal of Applied Psychology1984，69，1： | 1 |
| 14 | Ammonia-oxidizing bacteria in a chloraminated distribution system: seasonal occurrence, distribution and disinfection resistance显示文摘 | WOLFE R L LIEU N I IZAGUIRRE G | 1990 | Appl Environ Microbiol1990，56，2： | 1 |
| 15 | Inereased basic fibroblast growth factor levels in serum and blister fluid from patients with vitiligo显示文摘 | Ozdemir M Yillar G Wolf R | 2000 | Acta Derm Venereol2000，80，6： | 1 |
| 16 | Antecedents of organizational slack 显示文摘 | SHARFMAN M P WOLF G CHASE R B | 1988 | Academy of Management Review1988，13，4： | 1 |
| 17 | RWG: An Assessment of Within-group Interrater Agreement显示文摘 | JAMES L R DEMAREE R G WOLF G | 1993 | Journal of Applied Psychology1993，78，2： | 1 |
| 18 | Fine structure of Methanobacterium thermoautotrophicum:effect of growth temperature on morphology and ultrastructure显示文摘 | Zeikus J G Wolfe R S | 1973 | J Bacteriol1973，113，1： | 1 |
| 19 | Estimating within group interrater reliability with and without response bias显示文摘 | JAMES L R DEMAREE R G WOLF | 1985 | J Appl Psyehol1985，69，1： | 1 |
| 20 | Estimating Within-group Interrater Reliability With and Without Response Bias 显示文摘 | James L R Demaree R G Wolf G | 1984 | Journal of Applied Psychology1984，，69： | 1 |
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