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| 1 | Optimisation of Adventitious Shoot Regeneration and Agrobacterium-mediated Transformation in Canna × generalis(Canna Lily)显示文摘Canna being ornamental plants has a significant role in agriculture, medical, economy and food industry. Canna has a limited vase life due to the rapid loss of moisture from its perianth. For improving its market value, cuticularisation of the perianth can be achieved by the expression of a heterologous cutin producing gene, using the tissue culture and transformation protocol developed in this study. Efficient, rapid and direct adventitious shoot regeneration was successfully established in Canna × generalis using recalcitrant rhizome explants. The explants were cultured on MS medium supplemented with 6-benzylaminopurine(6-BA), thidiazuron(TDZ), and kinetin. Among the four genotypes taken for tissue culture, the ‘Trinacria variegata' was the best responding cultivar. And 2 mg · L^(-1)6-BA or 1.5 mg · L^(-1) TDZ along with 0.1 mg · L^(-1) IAA was optimum for their regeneration. The highest regeneration was achieved in ‘Trinacria variegata'(36%) on 6-BA, 33% on TDZ while kinetin failed to evoke any regenerative responses. Regeneration was enhanced by supplementation of 100 mg · L^(-1) ascorbic acid(AsA), while, 100 mg · L^(-1) of l-cysteine or 100 mg · L^(-1) dithiothreitol(DTT), inhibited regeneration. Shoots were observed to develop 3–5 fibrous roots on MS medium supplemented with 0.5 mg · L^(-1) indole-3-butyric acid(IBA). The plantlets were transplanted into pots and acclimatised in glasshouse with 100%survival. For transformation of Canna, rhizome explants were co-cultivated for 60 min in Agrobacterium suspension. The explants were washed with 500 mg · L^(-1) cefotaxime solution, subjected to 100 mg · L^(-1) kanamycin selection followed by excision of the shoots and culturing them on IBA-supplemented media for root development. Transgene integration in the putative transformants was confirmed by PCR assay and copy number by Southern blot hybridisation analysis. | Rani Singh Arvind Kumar Dubey Indraneel Sanyal | 2019 | Horticultural Plant Journal2019，5，1： | 6 |
| 2 | Assessment of tumor markers CA 19-9,CEA,CA 125,and CA 242 for the early diagnosis and prognosis prediction of gallbladder cancer显示文摘BACKGROUND Gallbladder cancer(GBC)is one of the leading and aggressive cancers in this region of India.It is very difficult to diagnose in the early stage,as it lacks typical early signs and symptoms;thus,the diagnosis is often in the advanced stage,which ultimately leads to a poor 5-year survival outcome.Tumor markers including carbohydrate antigen 19-9(CA 19-9),carcinoembryonic antigen(CEA),CA 125,CA 242,and alpha fetoprotein are used as indicators in the diagnosis and prognosis of GBC.AIM To compare tumor marker levels between GBC and benign GB diseases(GBDs)and to assess the combined use of tumor markers to increase the diagnostic accuracy for GBC.METHODS Patients of either sex aged≥18 years,with suspected GBC(GB polyp,irregular thick GB wall,GB mass,porcelain GB)on the basis of radiological imaging were included in this study.GB wall thickness using ultrasonography and tumor markers CEA,CA 125,CA 19-9,and CA 242 in all patients were recorded.All cases after surgical intervention were divided into two groups,GBC and benign GBD,according to histopathological examination findings.The cases were followed up and clinical findings,radiological findings,and levels of tumor markers were assessed.RESULTS A total of 200 patients were included in this study,of whom 80 patients had GBC and 120 patients had benign GBD.The median(interquartile range)age was 52.0(41.0-60.0)years and the majority of patients(132,66.0%)were women.Tumor markers including CA 19-9,CA 125,CEA,and CA 242 were significantly elevated in patients with GBC(P<0.001).There was a significant reduction in tumor markers at 3 and 6 mo from baseline(P<0.001).The mean survival of patients with normal and elevated levels of tumor markers CA 125,CA 19-9,and CEA was comparable;however lymph node metastasis and CA 242 expression level were independent prognostic factors.CONCLUSION Serum levels of tumor markers including CA 19-9,CA 125,CEA,and CA 242 were significantly associated with GBC.However,no significant association was observed between the presence of elevated levels of any tumor marker with respect to survival.Tumor marker assessment during follow-up may represent a treatment response. | Seema Rani Sinha Prem Prakash Rakesh Kumar Singh Dinesh Kumar Sinha | 2022 | World Journal of Gastrointestinal Surgery2022，14，11： | 5 |
| 3 | Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology显示文摘Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and Bangladesh.Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is ineffective.To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)pathotype.Using polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh.To test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR machine.Following this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 sequences.The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)activity.We then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat plants.This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field. | Houxiang Kang Ye Peng Kangyu Hua Yufei Deng Maria Bellizzi Dipali Rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang | 2021 | Engineering2021，7，9： | 4 |
| 4 | Potential applications of lactic acid bacteria and bacteriocins in antimycobacterial therapy显示文摘Tuberculosis(TB) is a communicable disease caused by Mycobacterium tuberculosis(M. tuberculosis). WHO estimated that 10.4 million new(incident) TB cases worldwide in year 2016. The increased prevalence of drug resistant strains and side effects associated with the current anti-tubercular drugs make the treatment options more complicated. Hence, there are necessities to identify new drug candidates to fight against various sub-populations of M. tuberculosis with less or no toxicity/side effects and shorter treatment duration. Bacteriocins produced by lactic acid bacteria(LAB) attract attention of researchers because of its 'Generally recognized as safe' status. LAB and its bacteriocins possess an effective antimicrobial activity against various bacteria and fungi. Interestingly bacteriocins such as nisin and lacticin 3147 have shown antimycobacterial activity in vitro. As probiotics, LAB plays a vital role in promoting various health benefits including ability to modulate immune response against various infectious diseases. LAB and its metabolic products activate immune system and thereby limiting the M. tuberculosis pathogenesis. The protein and peptide engineering techniques paved the ways to obtain hybrid bacteriocin derivatives from the known peptide sequence of existing bacteriocin. In this review, we focus on the antimycobacterial property and immunomodulatory role of LAB and its metabolic products. Techniques for large scale synthesis of potential bacteriocin with multifunctional activity and enhanced stability are also discussed. | Anbarasu Sivaraj Revathy Sundar Radhakrishnan Manikkam Krupakar Parthasarathy Uma Rani Vanaja Kumar | 2018 | Asian Pacific Journal of Tropical Medicine2018，11，8： | 2 |
| 5 | Soy Protein Isolate Film by Incorporating Mandelic Acid as Well as Through Fermentation Mediated by Bacillus Subtilis显示文摘Soy protein isolate(SPI)biopolymeric films were prepared by adding different contents of mandelic acid(1 to 5%wrt SPI)to glycerol plasticized SPI by solution casting method.Also,SPI was fermented by Bacillus subtilis to get fermented SPI films by solution casting.Molecular mass determination of mandelic acid incorporated and fermented SPI films was carried out by sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE).Mandelic acid incorporated and fermented SPI films were characterized by Fourier-transform infrared spectroscopy(FT-IR),dynamic mechanical analysis(DMA),tensile strength,water uptake and optical transmittance studies.Results indicated that incorporation of mandelic acid in SPI resulted in high tensile strength(8.03 MPa)and highα-relaxation(Tα)as well as low water uptake.On the other hand,films cannot be prepared from fermented SPI with SPI contents of 8%and 12%.However,film from fermented SPI with 16%SPI content could be prepared but it exhibited low tensile strength(3.18 MPa)and low Tαas well as high water uptake.The resulting mandelic acid incorporated SPI films were also subjected to antimicrobial studies.At all the concentration of mandelic acid,we can easily observe the antimicrobial effect in mandelic acid incorporated SPI films unlike fermented SPI films.This work will be helpful in fabricating antimicrobial SPI film from renewable resources. | Rakesh Kumar Priya Rani K.Dinesh Kumar | 2019 | Journal of Renewable Materials2019，7，2： | 2 |
| 6 | A Review and Analysis of Localization Techniques in Underwater Wireless Sensor Networks显示文摘In recent years,there has been a rapid growth in Underwater Wireless Sensor Networks(UWSNs).The focus of research in this area is now on solving the problems associated with large-scale UWSN.One of the major issues in such a network is the localization of underwater nodes.Localization is required for tracking objects and detecting the target.It is also considered tagging of data where sensed contents are not found of any use without localization.This is useless for application until the position of sensed content is confirmed.This article’s major goal is to review and analyze underwater node localization to solve the localization issues in UWSN.The present paper describes various existing localization schemes and broadly categorizes these schemes as Centralized and Distributed localization schemes underwater.Also,a detailed subdivision of these localization schemes is given.Further,these localization schemes are compared from different perspectives.The detailed analysis of these schemes in terms of certain performance metrics has been discussed in this paper.At the end,the paper addresses several future directions for potential research in improving localization problems of UWSN. | Seema Rani Anju Anupma Sangwan Krishna Kumar Kashif Nisar Tariq Rahim Soomro Ag.Asri Ag.Ibrahim Manoj Gupta Laxmi Chandand Sadiq Ali Khan | 2023 | Computers, Materials & Continua2023，，6： | 1 |
| 7 | Impacts of microwave pretreatments on the semi-continuous anaerobic digestion of dairy waste activated sludge显示文摘 | Rani R U Kumar S A Kaliappan S | 2013 | Waste Management2013，33，5： | 1 |
| 8 | MTHFR C677T polymorphism and its homocysteine‐driven effect on blood pressure显示文摘 | K. N. Saraswathy Priyanka Rani Garg Kabita Salam Shipra Joshi Suraj Singh Huidrom Pradeep Kumar Ghosh | 2014 | Int J Stroke2014，，： | 1 |
| 9 | Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea 显示文摘 | Singh K Kumar S Rani A | 2009 | Funct lntegr Genomics2009，9，： | 1 |
| 10 | Changes in lipoxygenase isozymes and trypsin inhibitor activity in soybean during germination at different temperatures 显示文摘 | Vineet Kumar Anita Rani | 2006 | Food Chemistry2006，99，3： | 1 |
| 11 | Daily expression of six clock genes in central and peripheral tissues of a night -migratory songhird: evidence for tissue-specific circadian timing显示文摘 | Singh D Rani S Kumar V | 2013 | Chronobiology international2013，30，： | 1 |
| 12 | Recent advances in solid-state fermentation显示文摘 | Reeta Rani Singhania Anil Kumar Patel Carlos R. Soccol Ashok Pandey | 2008 | Biochemical Engineering Journal2008，，1： | 1 |
| 13 | Long-range transport of aerosols from agriculture crop residue burning in Indo-Gangetic Plains:A study using LIDAR, ground mea- surements and satellite data显示文摘 | BADARINATH K V S KUMAR K S RANI S A | 2009 | Journal of Atmospheric and Solar-Terrestrial Physics2009，71，1： | 1 |
| 14 | Contribution of estrogen receptor subtypes,ERα,ERβ,and GPER1 in rapid estradiol-mediated enhancement of hippocampal synaptic transmission in mice显示文摘 | KUMAR A BEAN L A RANI A | 2015 | Hippocampus2015，25，12： | 1 |
| 15 | Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea显示文摘 | Singh K Kumar S Rani A Gulati A and Ahuja P S | | 0，，01： | 1 |
| 16 | Human Protein Reference Database- -2009 update显示文摘 | Keshava Prasad TS Goel R Kandasamy K Keerthikumar S Kumar S Mathivanan S Telikicherla D Raju R Shafreen B Venugopal A Balakrishnan L Mafimuthu A Banerjee S So- manathan DS Sebastian A Rani S Ray S Harrys Kishore C J Kanth S Ahmed M Kashyap MK Mohmood R Ramachandra YL Krishna V Rahiman BA Mohan S Ranganathan P Ram- abadran S Chaerkady R Pandey A | 2009 | Nucleic Acids Res2009，37，： | 1 |
| 17 | Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea 显示文摘 | Singh K Kumar S Rani A | 2009 | Funct Integr Genomics2009，9，1： | 1 |
| 18 | Recent advances in solid-state fermentation显示文摘 | Reeta Rani Singhania Anil Kumar Patel Carlos R Soccol | 2009 | Bio- chemical Engineering Journal2009，44，： | 1 |
| 19 | An Efficient Unsupervised Learning Approach for Detecting Anomaly in Cloud显示文摘The Cloud system shows its growing functionalities in various industrial applications.The safety towards data transfer seems to be a threat where Network Intrusion Detection System(NIDS)is measured as an essential element to fulfill security.Recently,Machine Learning(ML)approaches have been used for the construction of intellectual IDS.Most IDS are based on ML techniques either as unsupervised or supervised.In supervised learning,NIDS is based on labeled data where it reduces the efficiency of the reduced model to identify attack patterns.Similarly,the unsupervised model fails to provide a satisfactory outcome.Hence,to boost the functionality of unsupervised learning,an effectual auto-encoder is applied for feature selection to select good features.Finally,the Naïve Bayes classifier is used for classification purposes.This approach exposes the finest generalization ability to train the data.The unlabelled data is also used for adoption towards data analysis.Here,redundant and noisy samples over the dataset are eliminated.To validate the robustness and efficiency of NIDS,the anticipated model is tested over the NSL-KDD dataset.The experimental outcomes demonstrate that the anticipated approach attains superior accuracy with 93%,which is higher compared to J48,AB tree,Random Forest(RF),Regression Tree(RT),Multi-Layer Perceptrons(MLP),Support Vector Machine(SVM),and Fuzzy.Similarly,False Alarm Rate(FAR)and True Positive Rate(TPR)of Naive Bayes(NB)is 0.3 and 0.99,respectively.When compared to prevailing techniques,the anticipated approach also delivers promising outcomes. | P.Sherubha S.P.Sasirekha A.Dinesh Kumar Anguraj J.Vakula Rani Raju Anitha S.Phani Praveen R.Hariharan Krishnan | 2023 | Computer Systems Science & Engineering2023，45，4： | 1 |
| 20 | Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea 显示文摘 | Singh K Kumar S Rani A | 2009 | Funct lntegr Genomics2009，9，1： | 1 |
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