|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | AKT and ERK1/2 signaling in intrahepatic cholangiocarcinoma显示文摘Intrahepatic cholangiocarcinomas (ICC) are neoplasms that originate from cholangiocytes and can occur at any level of the biliary tree. Surgical resection is the current therapy of choice for this highly aggressive cancer. However, the 5-year survival still is poor, with high recurrence rates. Due to the intrahepatic growth a signifi cant proportion of patients present with advanced disease and are not candidates for curative surgery or transplantation. The existing palliative options are of limited benefit and there is a great necessity for novel therapeutic options. In this article we review the role of the phosphoinositide 3-kinase(PI3K)/ AKT and extracellular regulated kinase (ERK) signaling pathways in ICC and present new data on the prognostic value of these protein kinases. Finally, we discuss future upcoming therapeutic options based on targeting these signaling pathways. | KJ Schmitz H Lang J Wohlschlaeger GC Sotiropoulos H Reis KW Schmid HA Baba | 2007 | World Journal of Gastroenterology2007，13，48： | 8 |
| 3 | 中等收入国家年轻人脑卒中发病率增加:基于10年人口的研究显示文摘高收入国家年轻人脑卒中的发病率正在上升。然而,低收入和中等收入国家年轻人的脑卒中发病趋势仍未知。该研究旨在分析2005—2015年在巴西Joinville的年轻人的缺血性脑卒中(isehemic stroke,IS)和脑出血的发病趋势。 | 刘莉 叶鹏 Cabral NL Freire AT Conforto AB Dos Santos N Reis FI Nagel V Guesser VV Safanelli J Longo AL | 2017 | 中华高血压杂志2017，25，11： | 6 |
| 4 | Alpha-fetoprotein expression is a potential prognostic marker in hepatocellular carcinoma显示文摘AIM: To characterize the alpha-fetoprotein (AFP) positive and negative hepatocellular carcinoma (HCC) samples.METHODS: Thirty-seven paraffin-embedded human HCC samples were analyzed by immunohistochemistry for the following antigens: AFP, β-catenin, p53, CD44, MSH-2,MLH-1, and HNF-4. The tumors were divided into two groups based on the AFP expression. The immunophenotypic data and important clinical parameters were studied between the two groups.RESULTS: Twenty-one of the thirty-seven examined HCCs were AFP positive. Seven with nuclear p53 staining were AFP positive, while seven tumors with nuclear β-catenin staining were AFP negative. CD44 staining and high histological tumor grade were more frequent among the AFP-positive HCCs. The other immunophenotypical and dinical parameters did not show statistically significant difference in their distribution between the AFP positive and negative samples.CONCLUSION: AFP expression in HCC correlates with unfavorable prognostic factors, while nuclear β-catenin positivity is more common among the AFP-negative liver tumors. This observation supports the microarray data onin vivo human tumors. | Dénes Grg János Regly-Mérei Sándor Paku László Kopper Péter Nagy | 2005 | World Journal of Gastroenterology2005，11，32： | 6 |
| 5 | Bone mineral density in cone beam computed tomography:Only a few shades of gray显示文摘Cone beam computed tomography(CBCT) has often been used to determine the quality of craniofacial bone structures through the determination of mineral density, which is based on gray scales of the images obtained. However, there is no consensus regarding the accuracy of the determination of the gray scales in these exams. This study aims to provide a literature review concerning the reliability of CBCT to determine bone mineral density. The gray values obtained with CBCT show a linear relationship with the attenuation coefficients of the materials, Hounsfield Units values obtained with medical computed tomography, and density values from dual energy X-ray absorciometry. However, errors are expected when CBCT images are used to define the quality of the scanned structures be-cause these images show inconsistencies and arbitrari-ness in the gray values, particularly when related to abrupt change in the density of the object, X-ray beam hardening effect, scattered radiation, projection data discontinuity-related effect, differences between CBCTdevices, changes in the volume of the field of view(FOV), and changes in the relationships of size and position between the FOV and the object evaluated. A few methods of mathematical correction of the gray scales in CBCT have been proposed; however, they do not generate consistent values that are independent of the devices and their configurations or of the scanned objects. Thus, CBCT should not be considered the ex-amination of choice for the determination of bone and soft tissue mineral density at the current stage, par-ticularly when values obtained are to be compared to predetermined standard values. Comparisons between symmetrically positioned structures inside the FOV and in relation to the exomass of the object, as it occurs with the right and left sides of the skull, seem to be viable because the effects on the gray scale in the re-gions of interest are the same. | Marcio José da Silva Campos Thainara Salgueiro de Souza Sergio Luiz Mota Júnior Marcelo Reis Fraga Robert Willer Farinazzo Vitral | 2014 | World Journal of Radiology2014，6，8： | 3 |
| 6 | Advances in enhanced biological phosphorus removal: From micro to macro scale显示文摘 | Adrian Oehmen Paulo C. Lemos Gilda Carvalho Zhiguo Yuan Jürg Keller Linda L. Blackall Maria A.M. Reis | 2007 | Water Research2007，，11： | 3 |
| 7 | Mucins MUC1, MUC2, MUC5AC and MUC6 expressin in the evaluation of differentiation and clinico-biological behaviour of gastric carcinoma显示文摘 | Pinto-de-Sousa J David L Reis CA | 2002 | Virchows Arch2002，440，3： | 2 |
| 8 | Acute gouty synovitis associated with 'urate milk' 显示文摘 | FAM A G REIS M D SZALAI J P | 1997 | J Rheumatol1997，24，12： | 1 |
| 9 | Integrated transport and reaction in an ion exchange membrane bioreactor 显示文摘 | Velizarov S Reis M A M Crespo J G | 2002 | Desali- nation2002，149，13： | 1 |
| 10 | Therapeutic drug monitoring of racemic citalopram: a 5-year experience in Sweden, 1992-1997显示文摘 | REIS M LUNDMARK J BENGTSSON F | 2003 | Ther Drug Monit2003，25，2： | 1 |
| 11 | Processes used by good computational estimation 显示文摘 | Reys R E Rybolt J E | 1982 | Journal for Research in Mathematics Education1982，13，2： | 1 |
| 12 | Ascorbic acid enhances arsenic trioxide induced cytotoxicity inmuhiplemyeloma cells显示文摘 | Gad M Bahlis N J Reis I | | 0，，： | 1 |
| 13 | Dexmedetomidine: current role in anesthesia and intensive care显示文摘 | Afonso J Reis F | 2012 | Rev Bras Anestesiol2012，62，： | 1 |
| 14 | Competition between nitrate and nitrite reduction in denitrification by Pseudomonas fluorescens显示文摘 | Almeida J S Reis M Carrondo M | 1995 | Biotech Bioeng1995，46，： | 1 |
| 15 | Dexmedetomidine:current role in anes- thesia and intensive care 显示文摘 | Afonso J Reis F | 2012 | Rev Bras Anestesiol2012，62，1： | 1 |
| 16 | Vapor Bubble Departure in Forced Convection Boiling显示文摘 | Rei R Bernhard D M | 1993 | Int J Heat and Mass Transfer1993，36，： | 1 |
| 17 | Topiramate selectively decreases intracortical excitability in human motor cortex 显示文摘 | Reis J Tergau F Hamer HM | 2002 | Epilepsia2002，43，： | 1 |
| 18 | So,ware- controlled fault tolerance 显示文摘 | Reis G A Chang J Vachhar~ani N | 2005 | ACM Transactions on Architecture and Code Optimization2005，2，4： | 1 |
| 19 | Dexamethasone-loaded scaffolds prepared by supercritical-assisted phase inversion显示文摘 | Duarte A R C Mano J F Reis R L | 2009 | Acta Biomaterialia2009，5，6： | 1 |
| 20 | Osteochondral defects: Present situation and tissue engineering approaehes显示文摘 | MANO J F REIS R L | 2007 | Journal of Tissue Engineering and Regenerative Medicine2007，1，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员