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| 1 | Experimental small bowel preservation using Polysol: A new alternative to University of Wisconsin solution, Celsior and histidine-tryptophan-ketoglutarate solution?显示文摘AIM: To evaluate the potential of Polysol, a newly developed preservation solution, in cold storage of small bowel grafts, compared with the current standards, University of Wisconsin solution (UW), Celsior and histidine-tryptophan-ketoglutarate solution (HTK). METHODS: Male Wistar rats were used as donors. Small bowels were retrieved, flushed and then stored in the respective 4 solutions for 18 h at 4℃. Functional integrity of the grafts was evaluated by isolated reperfusion with oxygenated Krebs-Henseleit buffer at 37℃ for 30 min in all 4 groups. RESULTS: Polysol preservation exhibited the highest tissue ATP concentration and the lowest release of LDH. Malondialdehyde, an index for tissue lipid peroxidation, was also the lowest in Polysol. Tissue oxygen consumption was significantly higher in Polysol than in the others. Of interest, UW-storage promoted 10-fold higher apoptosis than in the others. Moreover, electron microscopy revealed that the mucosal villi/micro-villi formation and the cell organelles, including mitochondria, were both significantly better preserved in Polysol, while deleterious alterations were apparent in the others, most notably in UW. Although Celsior and HTK exhibited the better trend of results than UW in some parameters, but could not reach the over-all superiority to UW.CONCLUSION: Cold storage using Polysol resulted in significantly better integrity and function of small bowel grafts than UW. Hence, Polysol may be a novel alternative for the small bowel preservation. | Lai Wei Koichiro Hata Benedict Marie Doorschodt Reinhard Büttner Thomas Minor René H Tolba | 2007 | World Journal of Gastroenterology2007，13，27： | 9 |
| 2 | Molecular phenotypes of human parvovirus B19 in patients with myocarditis显示文摘AIM:To investigate molecular phenotypes of myocardial B19V-infection to determine the role of B19V in myocarditis and dilated cardiomyopathy(DCM).METHODS:Endomyocardial biopsies(EMBs) from 498 B19V-positive patients with myocarditis and DCMwere analyzed using molecular methods and functional experiments.EMBs were obtained from the University Hospitals of Greifswald and Tuebingen and additionally from 36 German cardiology centers.Control tissues were obtained at autopsy from 34 victims of accidents,crime or suicide.Identification of mononuclear cell infiltrates in EMBs was performed using immunohistological staining.Anti-B19V-IgM and anti-B19V-IgG were analyzed by enzyme-linked immunosorbent assay(ELISA).B19V viral loads were determined using in-house quantitative real-time polymerase chain reaction(PCR).For B19V-genotyping a new B19V-genotype-specific restriction fragment length polymorphism(RFLP)-PCR was established.B19V-genotyping was verified by direct DNAsequencing and sequences were aligned using BLAST and BioEdit software.B19V P6-promoter and HHV6-U94-transactivator constructs were generated for cell culture experiments.Transfection experiments were conducted using human endothelial cells 1.Luciferase reporter assays were performed to determine B19Vreplication activity.Statistical analysis and graphical representation were calculated using SPSS and Prism5 software.RESULTS:The prevalence of B19V was significantly more likely to be associated with inflammatory cardiomyopathy(iCMP) compared to uninflamed DCM(59.6% vs 35.3%)(P < 0.0001).The detection of B19V-mRNA replication intermediates proved that replication of B19V was present.RFLP-PCR assays showed that B19V-genotype 1(57.4%) and B19V-genotype 2(36.7%) were the most prevalent viral genotypes.B19V-genotype 2 was observed more frequently in EMBs with iCMP(65.0%) compared to DCM(35%)(P = 0.049).Although there was no significant difference in gender-specific B19V-loads,women were more frequently infected with B19V-genotype 2(44.6%) than men(36.0%)(P = 0.0448).Coinfection with B19V and other cardiotropic viruses was found in 19.2% of tissuesamples and was associated with higher B19V viral load compared to B19V-monoinfected tissue(P = 0.0012).The most frequent coinfecting virus was human herpes virus 6(HHV6,16.5%).B19V-coinfection with HHV6 showed higher B19V-loads compared to B19V-monoinfected EMBs(P = 0.0033),suggesting that HHV6 had transactivated B19V.In vitro experiments confirmed a 2.4-fold increased B19V P6-promoter activity by the HHV6 U94-transactivator.CONCLUSION:The finding of significantly increased B19V loads in patients with histologically proven cardiac inflammation suggests a crucial role of B19V-genotypes and reactivation of B19V-infection by HHV6-coinfection in B19V-associated iCMP.Our findings suggest that B19V-infection of the human heart can be a causative event for the development of an endothelial cell-mediated inflammatory disease and that this is related to both viral load and genotype. | C-Thomas Bock Anja Düchting Friederike Utta Eva Brunner Bui Tien Sy Karin Klingel Florian Lang Meinrad Gawaz Stephan B Felix Reinhard Kandolf | 2014 | World Journal of Cardiology2014，6，4： | 3 |
| 3 | New natural draft cooling tower of 200 m of height显示文摘 | BUSCH DIETER HARTE REINHARD KR:_TZIG WILFRIED B | 2002 | Engi- neering Structures2002，，24： | 1 |
| 4 | Dynamic cerebral autoregulation and collateral flow patterns inpatients with severe carotid stenosis or occlusion显示文摘 | Reinhard M Muller T Guschlbauer B | | 0，，8： | 1 |
| 5 | Color transfer between images显示文摘 | Reinhard E Ashikhmin M Gooch B | 2001 | IEEE Computer Graphics and Applications2001，21，5： | 1 |
| 6 | Color transfer between images显示文摘 | Reinhard E Ashikhmin M Gooch B | 2001 | IEEE Computer Graphics and Applications2001，21，5： | 1 |
| 7 | Color transfer between images 显示文摘 | Reinhard E Ashikhmin M Gooch B | 2001 | IEEE Computer Graphics and Applications2001，21，5： | 1 |
| 8 | Additive prognostic value of plasma N-terminal pro-brain natriuretic peptide and coronary artery calcification for cardiovascular events and mortality in asymptomaftic patients with type 2 diabetes 显示文摘 | yon Sehohen B J Reinhard H Hansen TW | 2015 | Cardiovasc Diabetol2015，23，14： | 1 |
| 9 | Investigating changes of electrical characteristics of the saturated zone affected by hazardous organic waste显示文摘 | REINHARD K PATRICK J THOMAS B | 2008 | Journal of Applied Geophysics2008，64，： | 1 |
| 10 | Color trausfer hetween images 显示文摘 | Reinhard E Ashikmin M Gooch B | 2001 | IEEE Computer Graphies and Applications2001，21，5： | 1 |
| 11 | Occurrence of lotaustralinin the genus Hevea and changes of HCN- potential in developing organs of Hevea brasiliensis 显示文摘 | REINHARD L B ADOLF N T DIRKS M | 1986 | Phytochemistry1986，25，7： | 1 |
| 12 | Color transfer between images显示文摘 | Reinhard E Ashikhmin M Gooch B | 2001 | IEEE Computer Graphics and Applications2001，21，5： | 1 |
| 13 | Narcotrend monitoring allows faster emergence and a reduction of drug consumption in propofol remifentanil anesthesia显示文摘 | Sascha K Andreas B Reinhard L | 2003 | Anesthesiology2003，99，1： | 1 |
| 14 | Biliary pneumonitis after proximal stent migration显示文摘 | Lutz Liebich-Bartholain Ulrich Kleinau Harry Elsbernd Reinhard Büchsel | 2001 | Gastrointestinal Endoscopy2001，，3： | 1 |
| 15 | High-purity CdMnTe radiation detectors: A high-resolution spectroscopic evaluation 显示文摘 | RAFIEI R REINHARD M KIM K PROKOPOVICH D BOARDMAN D SARBUTT A JAMES R B | 2013 | 1EEE Transactions on Nuclear Science2013，60，2： | 1 |
| 16 | Color transfer between images显示文摘 | Reinhard E Ashikhmin M Gooch B | 2001 | IEEE Computer Graphics and Appli- cations2001，21，5： | 1 |
| 17 | Deterministic aperiodic arrays of metal nanoparticles for surface-enhanced Raman scattering(SERS)显示文摘 | Gopinath A Boriskina S V Reinhard B M | 2009 | Opt Express2009，17，5： | 1 |
| 18 | Color transfer between images显示文摘 | Reinhard M Ashikhmin M Gooch B | 2001 | IEEE Computer Graphics and Applications2001，21，5： | 1 |
| 19 | Novel actin rings within the secretory cells of honeybee royal jelly glands显示文摘 | KHEYRI H CRIBB B W REINHARD J | 2012 | Cytoskele-ton2012，69，12： | 1 |
| 20 | Predictors of Outcome in Patients With Suspected Myocarditis显示文摘 | Ingrid Kindermann Michael Kindermann Reinhard Kandolf Karin Klingel Burkhard Bültmann Thomas Müller Angelika Lindinger Michael B?hm | 2008 | Circulation2008，，6： | 1 |
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