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| 1 | Relevance of α-defensins(HNP1-3) and defensin β-1 in diabetes显示文摘AIM: To investigate the genetic background of human defensin expression in type 1 and 2 diabetes.METHODS: Associations between DEFA1/DEFA3 gene copy number polymorphism and diabetes as well as between the promoter polymorphisms of DEFB1 and diabetes were studied. The copy number variation of the DEFA1/DEFA3 genes was determined in 257 diabetic patients(117 patients with type 1 and 140 with type 2 diabetes). The control group consisted of 221 age- and gender-matched healthy blood donors. The cumulative copy numbers of the DEFA1/DEFA3 genes were detected by using quantitative PCR analysis. To evaluate the HNP 1-3(human neutrophil peptide 1-3 or α-defensin) levels in the circulation, plasma HNP 1-3 concentrations were measured by ELISA. The expression of DEFA1/A3 in peripheral leukocytes of the diabetic patients was measured by quantitative RT PCR analysis. Three SNPs of the human DEFB1(human defensin β-1) gene: DEFB1 G-20A(rs11362), DEFB1 C-44G(rs1800972) and DEFB1 G-52A(rs1799946) were genotyped by Custom TaqMan? Real Time PCR assay.RESULTS: Significant differences were observed in HNP1-3 levels between the healthy subjects and both groups of diabetic patients. The mean ± SE was 28.78 ± 4.2 ng/mL in type 1 diabetes, and 29.82 ± 5.36 ng/mL in type 2 diabetes, vs 11.94 ± 2.96 ng/mL in controls; P < 0.01 respectively. There was no significant difference between patients with type 1 and type 2 diabetes in the high plasma concentrations of HNP1-3. The highest concentrations of α-defensin were found in diabetic patients with nephropathy(49.4 ± 4.8 ng/mL), neuropathy(38.7 ± 4.8 ng/mL) or cardiovascular complications(45.6 ± 1.45 ng/L). There was no significant difference in the cumulative copy numbers of DEFA1/DEFA3 genes between controls and patients, or between patients with the two types of diabetes. Comparisons of HNP 1-3 plasma level and DEFA1/A3 copy number of the same patient did not reveal significant relationship between defensin-α levels and the gene copy numbers(r2 = 0.01). Similarly, no positive correlation was observed between the copy numbers and the mRNA expression levels of DEFA1/A3. Regarding the C-44G polymorphism of DEFB1, the GG 'protective' genotype was much less frequent(1%-2%) among both groups of patients than among controls(9%).CONCLUSION: Elevated HNP1-3 levels in diabetes are independent of DEFA1/DEFA3 copy numbers, but GG genotype of C-44G SNP in DEFB1 gene may result in decreased defensin β-1 production. | Balázs Csaba Németh Tamás Várkonyi Ferenc Somogyvári Csaba Lengyel Katalin Fehértemplomi Szabolcs Nyiraty Péter Kempler Yvette Mándi | 2014 | World Journal of Gastroenterology2014，20，27： | 4 |
| 2 | A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants显示文摘 | Ling V Juliano RI | 1978 | Biochem Biophys Acta1978，455，： | 1 |
| 3 | Life cycle assessment of residential heating and cooling systems in four regions in the United States显示文摘 | Shah V P Debella D C Ries R J | 2008 | Energy and Buildings2008，40，4： | 1 |
| 4 | Pathogenesis and treatment of idiopathic nephrotic syndrome in adults 显示文摘 | SAHALI D AUDARD V RI~MY P | 2012 | Nephrol Ther2012，8，3： | 1 |
| 5 | Treatment of primary Sj6gren's syndrome with hydroxychloroquine: a retrospective, open-label study显示文摘 | Fox RI Dixon R Guarrasi V | 1996 | Lupus1996，5，1： | 1 |
| 6 | Diffusion tensor MRI of the spinal cord显示文摘 | Ries M Jones RA Dousset V | 2000 | Magn Reson Med2000，44，16： | 1 |
| 7 | Advanced shape memory alloys processed by powder metallurgy显示文摘 | PI~RI~SAEZ R B RECARTE V N(SM L | 2000 | Advanced Engineering Materials2000，2，12： | 1 |
| 8 | Polymerase chain reac- tion amplification and typing of rotavirus nucleic acid from stool specimens 显示文摘 | Gouvea V Glass RI Woods P | 1990 | J Clin Microbiol1990，28，2： | 1 |
| 9 | A complete α1,3-galactosyltransferase gene is present in the human genome and partially transcribed显示文摘 | Lantéri M Giordanengo V Vidal F | 2002 | Glycobiology2002，12，12： | 1 |
| 10 | Reactions of propaneon supported Mo2C catalysts显示文摘 | Solymosi F Németh R óvári L | 2000 | Journal of Catalysis2000，195，2： | 1 |
| 11 | MMP-2,MT1-MMP,and TIMP-2 are essential for the invasive capacity of human mesenchymal stem cells:differential regulation by inflammatory cytokines显示文摘 | Ries C Egea V Karow M | | 0，，： | 1 |
| 12 | Triacontanol: A new naturally occurring plant growth regulator显示文摘 | Ries S Wert V Sweeley C C | 1977 | Science1977，195，： | 1 |
| 13 | Triacontanol:a newnaturally occurring plant growth regulator 显示文摘 | RIES S WERT V SWEELEY C C | 1977 | Science1977，195，： | 1 |
| 14 | Activity of interferon alpha, intedeukin 6 and insulin in the regulation of differentiation in A549 alveolar carcinoma cells显示文摘 | McCormick C Freshney RI Speirs V | 1995 | Br J Cancer1995，71，： | 1 |
| 15 | Triacontanol: A New Naturally Occurring Plant Growth Regulator 显示文摘 | Ries S K Wert V Sweeley C C | 1977 | Science1977，195，4284： | 1 |
| 16 | Comparison and evaluation of methods for liver segmentation from CT datasets显示文摘 | Heimann T van Ginneken B Styner MA Arzhaeva Y Aurich V Bauer C Beck A Becket C Beichel R Bekes G Bello F Binnig G Bischof H Bornik A Cashman PM Chi Y Cordova A Dawant BM Fidrich M Furst JD Furukawa D Grenacher L Hornegger J Kainmuller D Kitney RI Kobatake H Lamecker H Lange T Lee J Lennon B Li R Li S Meinzer HP Nemeth G Raicu DS Rau AM van Rikxoort EM Rousson M Rusko L Saddi KA Schmidt G Seghers D Shimizu A Slagmolen P Sorantin E Soza G Susomboon R Waite JM Wimmer A Wolf I | 2009 | IEEE Transactions on Medical Imaging2009，28，8： | 1 |
| 17 | MMP-2,MT1,MMP,and TIMP-2 are essential forthe invasive capacity of human mesenehymalstem cells:differential regulation by inflammatory cytokines显示文摘 | Ries C Egea V Karow M | 2007 | Blood2007，109，9： | 1 |
| 18 | Leptin accelerates autoimmune diabetes in female NOD mice显示文摘 | Sanna V Lechler RI | 2002 | Diabetes2002，51，5： | 1 |
| 19 | Medical humani- ties at New York University School of Medicine: an array of rich programs in diverse settings 显示文摘 | Krackov SK Levin RI Catanese V etal | 2003 | Acad Med2003，78，10： | 1 |
| 20 | Classification of IntentionalElectromagnetic Environments (IEME) 显示文摘 | G TESCHE F M | 2004 | IEEE Tran-scations on Electromagnetic Compatibility2004，46，3： | 1 |
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