|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 动态文化为骨头工程作为脚手架在 freeze-dried 骨头上改进 MSC 粘附显示文摘 AIM:To investigate the interaction between mesenchymal stem cells(MSCs) and bone grafts using two different cultivation methods:static and dynamic.METHODS:MSCs were isolated from rat bone marrow.MSC culture was analyzed according to the morphology,cell differentiation potential,and surface molecular markers.Before cell culture,freeze-dried bone(FDB) was maintained in culture for 3 d in order to verify culture medium pH.MSCs were co-cultured with FDB using two different cultivation methods:static co-culture(two-dimensional) and dynamic co-culture(threedimensional).After 24 h of cultivation by dynamic or static methods,histological analysis of Cell adhesion on FDB was performed.Cell viability was assessed by the Trypan Blue exclusion method on days 0,3 and 6 after dynamic or static culture.Adherent cells were detached from FDB surface,stained with Trypan Blue,and quantified to determine whether the cells remained on the graft surface in prolonged non-dynamic culture.Statistical analyses were performed with SPSS and a P < 0.05 was considered significant.RESULTS:The results showed a clear potential for adipogenic and osteogenic differentiation of MSC cultures.Rat MSCs were positive for CD44,CD90 and CD29 and negative for CD34,CD45 and CD11bc.FDBs were maintained in culture for 3 d and the results showed there was no significant variation in the culture medium pH with FDB compared to pure medium pH(P > 0.05).In histological analysis,there was a significant difference in the amount of adhered cells on FDB between the two cultivation methods(P < 0.05).The MSCs in the dynamic co-culture method demonstrated greater adhesion on the bone surface than in static co-culture method.On day 0,the cell viability in the dynamic system was significantly higher than in the static system(P < 0.05).There was a statistical difference in cell viability between days 0,3 and 6 after dynamic culture(P < 0.05).In static culture,cell viability on day 6 was significantly lower than on day 3 and 0(P < 0.05).CONCLUSION:An alternative cultivation method was developed to improve the MSCs adhesion on FDB,demonstrating that dynamic co-culture provides a superior environment over static conditions. | Fabiany da Costa Gonalves Ana Helena da Rosa Paz Priscila Schmidt Lora Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima | 2012 | World Journal of Stem Cells2012，4，2： | 2 |
| 2 | Height-to-diameter ratios with temporal and dendro/morphometric variables for Brazilian pine in south Brazil显示文摘Height-to-diameter ratios(HD)are an important measure of the stability,density and competition of forest stands.It refl ects the vertical growth of the trees,the vulnerability of the forest canopy structure and infl uences volumetric production.HD ratios vary according to tree size,availability of resources for growth,stand density and species composition.Data were taken from 210 trees and a regression technique of generalized linear models for the HD ratio applicable for forest structure conservation was developed.The objective of this study was to model the HD ratios of dominant and co-dominant trees of Araucaria angustifolia according to morphometric,dendrometric,annual diameter increment,stand density,and age variables in three sites in southern Brazil.The results show that the HD ratio decreases with increasing age,crown area and basal area,and increases with stand density and annual diameter increment.Accuracy of the developed equations was demonstrated by the values of deviation,Bayesian and Akaike criteria.The results are of interest to forest managers since they make decisions about silvicultural operations.Growth continuity and forest production indicate that any intervention should be directed at younger trees of smaller sizes,and that one of the main management factors for stand stability and growth is the formation of the stand and its capture of light. | Andre Felipe Hess Myrcia Minatti Emanuel Arnoni Costa Luis Paulo Baldissera Schorr Gabriel Teixeira da Rosa Isadora de Arruda Souza Geedre Adriano Borsoi Veraldo Liesenberg Thiago Floriani Stepka Roberta Abatti | 2021 | Journal of Forestry Research2021，32，1： | 2 |
| 3 | Effect of heat-moisture treatment on rice starch of varying amylose content显示文摘 | Elessandra da Rosa Zavareze Cátia Regina Storck Luis Antonio Suita de Castro Manoel Artigas Schirmer Alvaro Renato Guerra Dias | 2009 | Food Chemistry2009，，2： | 1 |
| 4 | The Antioxidant Activity of Coumarins and Flavonoids显示文摘 | Guilherme Borges Bubols Damiana da Rocha Vianna Alexander Medina-Remon Gilsane von Poser Rosa Maria Lamuela-Raventos Vera Lucia Eifler-Lima Solange Cristina Garcia | 2013 | Mini-Reviews in Medicinal Chemistry2013，，3： | 1 |
| 5 | Severe CD4 T cell-mediated immunopathology in murine schistosomiasis is de- pendent on IL-12p40 and correlates with high levels of IL-17 显示文摘 | Rutitzky L I Lopes da Rosa J R Stadecker M J | 2005 | J Immunol2005，175，6： | 1 |
| 6 | Content of major classes of polyphenolic compounds,antioxidant,antiproliferative,and cell protective activity of pecan crude extracts and their fractions显示文摘 | DA LA ROSA L A VAZQUEZ-FLORES A A ALVAREZPARRILLA E | 2014 | Journal of Functional Foods2014，7，2： | 1 |
| 7 | Epidemiology of sporotrichosis: A study of 304 cases in Brazil 显示文摘 | Da Rosa ACM Scroferneker ML Vettorato R | 2005 | J Am Acad Derm2005，52，45： | 1 |
| 8 | Severe CD4 T Cell- mediated immunopathology in murine schistosomiasis is dependent on IL- 12p40 and correlates with high levels of IL-17 显示文摘 | Rutitzky L I Lopes da Rosa J R Stadecker M J | 2005 | J Immunol2005，175，6： | 1 |
| 9 | Fabrication of highly porous yttria-stabilized zirconia by acid leaching nickel from a nickel-yttria-stabilized zirconia cerme显示文摘 | Hyuk Kim Calvin Da Rosa | 2002 | Journal of the American Ceramic Socity2002，85，6： | 1 |
| 10 | Photoluminescence emissions of Ca_(1-x)WO_(4):xEu^(3+):Bridging between experiment and DFT calculations显示文摘In this work,the impact of the doping process on the photoluminescence emission of CaWO_(4) as a function of the concentration of Eu^(3+) cation(0.01 mol%,0.02 mol%,0.04 mol%,0.06 mol%,0.08 mol%,and 0.10 mol%) is discussed in detail.Ca_(1-x)WO~4:xEu^(3+) samples were successfully synthesized by a simple coprecipitation method followed by microwave irradiation.The blue shift in the absorption edge confirms the quantum confinement effect and the band gap energy covers the range from 3.91 to 4.18 eV,as the amount of Eu^(3+) cations increases.The experimental results are sustained by first-principles calculations,at the density functional theory level,to decipher the geometry and electronic properties,thereby enabling a more accurate and direct comparison between theory and experiment for the Ca_(1-x)WO_(4):xEu^(3+) structure. | Amanda Fernandes Gouveia Marcelo Assis Lara Kelly Ribeiro Aline Estefany Brandao Lima Eduardo de Oliveira Gomes Daniele Souza Yara Gobato Galvao Ieda Lucia Viana Rosa Geraldo Eduardo da Luz Jr Eva Guillamón Elson Longo Juan Andrés Miguel Angel San-Miguel | 2022 | Journal of Rare Earths2022，40，10： | 1 |
| 11 | Extraesophageal manifestations of gastroesophageal reflux disease 显示文摘 | Gurski RR da Rosa AR do Valle E | 2006 | J Bras Pneumol2006，32，2： | 1 |
| 12 | Promoter hypermethylation patterns of p16,O6-methylguanine-DNA-methyltransferase, and death-associated protein kinase in tumors and saliva of head and neck cancer patients 显示文摘 | Rosas SL Koch W da Costa Carvalho MG | 2001 | Cancer Res2001，61，3： | 1 |
| 13 | The sharemarket performance of Australian venture capital-backed and non-venture capital-backed IPOs 显示文摘 | Rosa Ray da Silva Gerard Velayuthen & TerryWalterc | 2003 | Pacific-Basin Finance Journal2003，，11： | 1 |
| 14 | The effect of insulin on macrophage metabolism and function显示文摘 | Costa Rosa LF Safi DA Cury Y | 1996 | Cell Biochem Funct1996，14，： | 1 |
| 15 | Thermal denaturation:is solid-state fermentation really a good technology for the production of enzymes显示文摘 | Marcelo Muller dos Santos Alexandre Souza da Rosa | 2004 | Bioresource Technology2004，93，： | 1 |
| 16 | Promoter hypermethylation patterns of p16,O6-methylguanine-DNA-methyltransferase,and death-associated protein kinase in tumors and saliva of head and neck cancer patients显示文摘 | ROSAS SL KOCH W da COSTA CARVALHO MG | 2001 | Cancer Res2001，61，3： | 1 |
| 17 | Oxidative stress and erythrocyte acetylcholinesterase (ACHE) in hypertensive and ischemie patients of both acute and chronic stages显示文摘 | Correa Mde C Maldonado P da Rosa CS | 2008 | Biomed Pharmacother2008，62，5： | 1 |
| 18 | Accuracy of serum hu- man epididymis protein 4 in ovarian cancer diagnosis: a systematic review and meta-analysis 显示文摘 | Macedo AC da Rosa MI Lumertz S | 2014 | Int J Gynecol Cancer2014，24，7： | 1 |
| 19 | Severe CD4 T cell-mediated immunopathology in murine schistosomiasis is dependent on IL-12p40 and correlates with high levels of IL-17 显示文摘 | Rutitzky L I Lopes da Rosa J R Stadecker M J | 2005 | J Immunol2005，175，6： | 1 |
| 20 | Teaching NeuroImages: Isolated hypothalamic hamartoma vs Pallister-Hall syndrome: Imaging and clinical correlation显示文摘 | Antonio J. da Rocha Marcos Rosa Junior Fernando Norio Arita | 2012 | Neurology2012，，9： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员