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| 1 | Effect of anti-biofilm glass–ionomer cement on Streptococcus mutans biofilms显示文摘Dental restorative materials with antimicrobial properties can inhibit bacterial colonization, which may result in a reduction of caries at tooth-filling interaction zones. This study aimed to develop antibacterial glass–ionomer cements(GIC) containing a quaternary ammonium monomer(dimethylaminododecyl methacrylate, DMADDM), and to investigate their effect on material performance and antibacterial properties. Different mass fractions(0, 1.1% and 2.2%) of DMADDM were incorporated into the GIC. The flexure strength, surface charge density, surface roughness and fluoride release were tested. A Streptococcus mutans biofilm model was used. Exopolysaccharides(EPS) staining was used to analyze the inhibitory effect of DMADDM on the biofilm matrix. In addition, biofilm metabolic activity, lactic acid metabolism and the expression of glucosyltransferase genes gtf B, gtf C and gtf D were measured. GIC containing 1.1% and 2.2% DMADDM had flexural strengths matching those of the commercial control(P40.1). DMADDM was able to increase the surface charge density but reduced surface roughness(Po0.05). The incorporation of 1.1% and 2.2% DMADDM elevated the release of fluoride by the GIC in the first 2 days(Po0.05). The novel DMADDM-modified GIC significantly reduced biofilm metabolic activity(Po0.05) and decreased lactic acid production(Po0.05). The quantitative polymerase chain reaction(q PCR) results showed that the expression of gtf B, gtf C and gtf D decreased when mass fractions of DMADDM increased(Po0.05). EPS staining showed that both the bacteria and EPS in biofilm decreased in the DMADDM groups. The incorporation of DMADDM could modify the properties of GIC to influence the development of S. mutans biofilms. In this study, we investigated the interface properties of antibacterial materials for the first time. GIC containing DMADDM can improve material performance and antibacterial properties and may contribute to the better management of secondary caries. | Su-Ping Wang Yang Ge Xue-Dong Zhou Hockin HK Xu Michael D Weir Ke-Ke Zhang Hao-Hao Wang Matthias Hannig Stefan Rupf Qian Li Lei Cheng | 2016 | International Journal of Oral Science2016，8，2： | 5 |
| 2 | Peroxidase reaction as a parameter for discrimination of streptococcus mutans and streptococcus sobrinus显示文摘 | Rupf S Merte K Eschrich K | 2001 | Caries-Res2001，35，4： | 1 |
| 3 | Comparison of profiles ofkey periodontal pathogens in periodontium and endodontium显示文摘 | Rupf S Kannengiesser S Merte K et a1 | 2000 | Endod Dent Traumantoi2000，16，6： | 1 |
| 4 | A three-step purification method of large quantities of human recombinant alpha endothelial cellular growth factor for clinical use 显示文摘 | Sauter A Lambert K L Rupf A K | 2007 | International Journal of Molecular Medicine2007，19，1： | 1 |
| 5 | Quantification of bacteria inoral samples by competitive polymerase chain reaction 显示文摘 | Rupf S Merte K Eschrich K | 1999 | JDent Res1999，78，： | 1 |
| 6 | On the search for good aperiod- ic binary invertible sequences 显示文摘 | RUPRECHT J RUPF M | 1996 | IEEE Transactions on Information Theory1996，42，5： | 1 |
| 7 | Intergranular Corrosion of UNS S31803 Heat Treated at 800 ℃ Varying Range Times显示文摘 | Najara Barros Dias Bianca Barros dos Santos Pedro Rupf Pereira Vlana | 2015 | Journal of Chemistry and Chemical Engineering2015，9，8： | 1 |
| 8 | Conrtmrison of profiles of key periodontal pathogens in pefiodontium and endodontium 显示文摘 | Rupf S Kannengiesser S Merte K | 2000 | Endod Dent Traumatol2000，16，6： | 1 |
| 9 | In vitro, clinical, and mi- crobiological evaluation of a linear oscillating device for scaling and root planning 显示文摘 | Rupf S Brader I Vonderlind D | 2005 | J Periodontol2005，76，11： | 1 |
| 10 | Comparison of profiles of key periodontal pathogens in periodontium and ell -dodontlum 显示文摘 | Rupf S Kannengiesser S Merte K | 2000 | Endod Dent Traumatol2000，16，6： | 1 |
| 11 | Some mathematical results in the pricing of American options显示文摘 | Dewynne J N Howison S D Rupf I | 1973 | European Journal of Applied Mathematics1973，4，： | 1 |
| 12 | Comparision of different techniques of quantitative PCR for determination of streptococcus mutans counts in saliva samples显示文摘 | Rupf S Merte K Kneist S | 2003 | Oral Microbiol Immunol2003，18，1： | 1 |
| 13 | Intergranular Corrosion of AISI 304 Heat Treated at 800℃ Varying Range Times显示文摘 | Pedro Rupf Pereira Viana Luiza Venturini Yasmin Siqueira Souza | 2015 | Journal of Chemistry and Chemical Engineering2015，9，4： | 1 |
| 14 | Comparison of profiles of key periodontal pathogens in periodontium and endodontium 显示文摘 | Rupf S Kannengiesser S Merte K | 2000 | Endod Dent Traumatol2000，16，： | 1 |
| 15 | Comparison of profiles of key periodontal pathogeus in periodontium and endodontium显示文摘 | Rupf S Kannengiesser S Merte K | 2000 | Endod Dent Traumatol2000，16，6： | 1 |
| 16 | Effects of imatinib on monocyte- derived dendritic cells are mediated by inhibition of nuclear factor- kappaB and Akt signaling pathways显示文摘 | Appel S Rupf A Week MM | 2005 | Clin Cancer Res2005，11，5： | 1 |
| 17 | Comparison of profiles of key periodontal pathogens in periodontium and endedontium显示文摘 | Rupf S Kannengiesser S Merte K | 2000 | Endod Dent Traumatol2000，16，6： | 1 |
| 18 | Comparison of profiles of key periodontal pathogens in periodontium and endodontium 显示文摘 | RUPF S KANNENGIESSER S MERTE K | 2000 | Endod Dent Traumatol2000，16，6： | 1 |
| 19 | In vitro, clinical, and microbiological evaluation of a linear oscillating device for scaling and root planing 显示文摘 | Rupf S Brader I Vonderlind D et ol | 2005 | JPeriodontol2005，76，11： | 1 |
| 20 | Comparison of pro- files of key periodontal pathogens in periodontium and endodonti- um 显示文摘 | Rupf S Kannengiesser S Mevte K | 2000 | Endod Dent Traumatol2000，16，6： | 1 |
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