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| 1 | In vivo radiometric analysis of glucose uptake and distribution in mouse bone显示文摘Bone formation and remodeling occurs throughout life and requires the sustained activity of osteoblasts and osteoclasts,particularly during periods of rapid bone growth.Despite increasing evidence linking bone cell activity to global energy homeostasis,little is known about the relative energy requirements or substrate utilization of bone cells.In these studies,we measured the uptake and distribution of glucose in the skeleton in vivo using positron-emitting^(18)F-fluorodeoxyglucose([^(18)F]-FDG) and non-invasive,high-resolution positron emission tomography/computed tomography(PET/CT) imaging and ex vivo autoradiography.Assessment of [^(18)F]-FDG uptake demonstrated that relative to other tissues bone accumulated a significant fraction of the total dose of the glucose analog.Skeletal accumulation was greatest in young mice undergoing the rapid bone formation that characterizes early development.PET/CT imaging revealed that [^(18)F]-FDG uptake was greatest in the epiphyseal and metaphyseal regions of long bones,which accords with the increased osteoblast numbers and activity at this skeletal site.Insulin administration significantly increased skeletal accumulation of [^(18)F]-FDG,while uptake was reduced in mice lacking the insulin receptor specifically in osteoblasts or fed a high-fat diet.Our results indicated that the skeleton is a site of significant glucose uptake and that its consumption by bone cells is subject to regulation by insulin and disturbances in whole-body metabolism. | Meredith L Zoch Diane S Abou Thomas L Clemens Daniel LJ Thorek Ryan C Riddle | 2016 | Bone Research2016，4，1： | 9 |
| 2 | Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage显示文摘Background: Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results. The purpose of this study was to identify the most stable housekeeping genes in porcine articular cartilage subjected to a mechanical injury from a panel of 10 candidate genes. Results: Ten candidate housekeeping genes were evaluated in three different treatment groups of mechanically impacted porcine articular cartilage. The genes evaluated were: beta actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hydroxymethylbilane synthase, hypoxanthine phosphoribosyl transferase, peptidylprolyl isomerase A (cyclophilin A), ribosomal protein L4, succinate dehydrogenase flavoprotein subunit A, TATA box binding protein, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein-zeta polypeptide. The stability of the genes was measured using geNorm, BestKeeper, and NormFinder software. The four most stable genes measured via geNorm were (most to least stable) succinate dehydrogenase flavoprotein, subunit A, peptidylprolyl isomerase A, glyceraldehyde-3-phosphate dehydrogenase, beta actin; the four most stable genes measured via BestKeeper were glyceraldehyde-3-phosphate dehydrogenase, peptidylprolyl isomerase A, beta actin, succinate dehydrogenase flavoprotein, subunit A; and the four most stable genes measured via NormFinder were peptidylprolyl isomerase A, succinate dehydrogenase flavoprotein, subunit A, glyceraldehyde-3-phosphate dehydrogenase, beta actin. Conclusions: BestKeeper, geNorm, and NormFinder all generated similar results for the most stable genes in porcine articular cartilage. The use of these appropriate reference genes will facilitate accurate gene expression studies of porcine articular cartilage and suggest appropriate housekeeping genes for articular cartilage studies in other species. | Ryan S McCulloch Melissa S Ashwell Audrey T O'Nan Peter L Mente | 2012 | Journal of Animal Science and Biotechnology2012，3，4： | 2 |
| 3 | The development of working memory in normally achieving and subtypes of learning disabled children显示文摘 | Siegel L S Ryan E B | | 0，，04： | 1 |
| 4 | NCCN Clinical Practice Guidelines in Oncology:rectal cancer 显示文摘 | Engstrom PF Arnoletti JP Benson AB 3rd Chen YJ Choti MA Cooper HS Covey A Dilawari RA Early DS Enzinger PC Fakih MG Fleshman J Jr Fuchs C Greta JL Kiel K Knol JA Leong LA Lin E Mulcahy MF Rao S Ryan DP Saltz L Shibata D Skibber JM Sofocleous C Thomas J Venook AP Willett C | 2009 | J Natl Compr Canc Netw2009，7，： | 1 |
| 5 | Barley 13-glucan extracts as rich sources o显示文摘 | Thondre P S Ryan L Henry C J K | 2011 | Food Chemis- try2011，126，1： | 1 |
| 6 | Rodent models of group 1 pulmonary hypertension 显示文摘 | Ryan J J Marsboom G Archer S L | 2013 | Handbook of experimental pharmacology2013，218，： | 1 |
| 7 | Catalytic conversion of biomass to monofunctional hydrocarbons and targeted liquid-fuel classes显示文摘 | EDWARD L K DANTE A S RYAN M W | 2008 | Science2008，322，5900： | 1 |
| 8 | Effects of PH on degradation of trichloroethylene by zero-valent iron显示文摘 | Chen J L Al-Abed S R Ryan J A | 2001 | Journal of Hazardous Materials B2001，83，： | 1 |
| 9 | Plasma polymerization of sputtered poly (tetrafluoroethylene) 显示文摘 | Ryan M E Fonseca J L Task S | 1995 | Journal of Physical Chemstry1995，99，18： | 1 |
| 10 | Antimicrobial peptides in the airway 显示文摘 | Laube D M Yim S Ryan L K | 2006 | Curr Top Microbiol Immunol2006，306，： | 1 |
| 11 | Organ preservation显示文摘 | Hicks M Hing A Gao L Ryan J Macdonald P S | 2006 | Methods Mol Biol2006，333，： | 1 |
| 12 | Rheological behavior and gel- point determination for a model Lewis acid-initiated chain growth epoxy resin 显示文摘 | Mortimer S Ryan A J Stanford J L | 2001 | Macromolecules2001，34，： | 1 |
| 13 | Enzymatic characterization of c-Met receptor tyrosine kinase oncogenic mutants and kinetic studies with aminopyridine and triazolopyrazine inhibitors 显示文摘 | Timofeevski S L Mctigue M A Ryan K | 2009 | Biochemistry2009，48，23： | 1 |
| 14 | Pitting corro- sion in cast 7 X X X aluminum alloys and fibre rein- forced MMCs显示文摘 | Winkler S L Ryan M P Flower H M | 2004 | Corrosion Science2004，46，4： | 1 |
| 15 | Sensitive and Robust One-Tube Real-Time Reverse Transcriptase-Polymerase Chain Reaction to Quantify SIV RNA Load:Comparison of One-versus Two-Enzyme Systems显示文摘 | Regina H L Ryan K S Vladimir L | 2000 | AIDS2000，16，13： | 1 |
| 16 | Molecular comparisons of Salsola tragus from California and Ukraine显示文摘 | Ryan F J Mosyakin S L Pitcairn M J | 2007 | Canadian Journal of Botany2007，85，2： | 1 |
| 17 | Effects of pH on dechlorination of trichloroethylene by zero-valent iron 显示文摘 | Chen J L A1-Abed S R Ryan J A | 2001 | Journal of Hazardous Materials2001，83，3： | 1 |
| 18 | Two dissimilar N-Acyl-Homoserine lactone acylases of pseudomonas syringae influence colony and biofilm morphology 显示文摘 | Ryan W S Steven E L | 2009 | Applied and Environmental Microbiology2009，71，1： | 1 |
| 19 | Shielding against micrometeoroid and orbital debris impact with metallic foams显示文摘 | Ryan S Christiansen E L Lear D M | 2010 | Orbital Debris Quarterly News2010，14，1： | 1 |
| 20 | Molecular analysis of programmed cell death during senescence in Arabidopsis thaliana and Brassica oleracea:cloning broccoli LSD1,Bax inhibitor and serine palmitoyltransferase homologues显示文摘 | COUPE S A WATSON L M RYAN D J | 2003 | J Experimental Botany2003，55，394： | 1 |
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