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	题名	作者	年代	出处	被引量
1	Differential reactivity of mouse monoclonal anti-HBs antibodies with recombinant mutant HBs antigens显示文摘AIM: To investigate the reactivity of a panel of 8 mouse anti-hepatitis B surface antigen (HBsAg) monoclonal antibodies (mAbs) using a collection of 9 recombinant HBsAg mutants with a variety of amino acid substitutions mostly located within the “a” region.METHODS: The entire HBs genes previously cloned into a mammalian expression vector were transiently transfected into COS7 cells. Two standard unmutated sequences of the ayw and adw subtypes served as controls. Secreted mutant proteins were collected and measured by three commercial diagnostic immunoassays to assess transfection efficiency. Reactivity of anti-HBs mAbs with mutated HBsAgs was determined by sandwich enzyme-linked immunosorbent assay (ELISA).RESULTS: Reactivity of anti-HBs mAbs with mutated HBsAgs revealed different patterns. While three mutants reacted strongly with all mAbs, two mutants reacted weakly with only two mAbs and the remaining proteins displayed variable degrees of reactivity towards different mAbs. Accordingly, four groups of mAbs with different but overlapping reactivity patterns could be envisaged. One group consisting of two mAbs (37C5-S7 and 35C6-S11) was found to recognize stable linear epitopes conserved in all mutants. Mutations outside the “a” determinant at positions 120 (P→S), 123(T→N) and 161(M→T) were found to affect reactivity of these mAbs.CONCLUSION: Our findings could have important implications for biophysical studies, vaccination strategies and immunotherapy of hepatitis B virus (HBV) mutants.	Azam Roohi Yaghoub Yazdani Jalal Khoshnoodi Seyed Mohammad Jazayeri William F Carman Mahmood Chamankhah Manley Rashedan Fazel Shokri	2006	World Journal of Gastroenterology2006，12，33：	4
2	Association of small vessel disease with dilatative arteriopath of the brain:neuropathologic evidence显示文摘	Pico F Labreuche J Sei Ihean D	2007	Stroke2007，38，9：	1
3	Vehicle routing problem with uncertain demands:An advanced particle swarm algorithm显示文摘	Babak F M Rubén R Seyed J S	2011	Computers Industrial Engineering2011，62，1：	1
4	Review of flexible riser modeling and analysis techniques显示文摘	Patel M H Seyed F b	1995	Engineering Structure1995，17，4：	1
5	Locomotive assignment problem with train precedence using genetic algorithm 显示文摘	SIAMAK N SEYED F	2012	Journal of Industrial Engineering International2012，8，1：	1
6	Internal flow-induced behavior of flexible risers显示文摘	Patel M H Seyed F B	1989	Engineering Structures1989，11，4：	1
7	Effects of high altitude and cold air exposure on airway inflammation in patients with asthma 显示文摘	Seys S F Daenen M Dilissen E	2013	Thorax2013，68，10：	1
8	Separation of 1-lysine from dilute aqueous solution using molecular imprinting technique显示文摘	Reza P Ebrahim V F Seyed A S	2007	Biochemical Engineering Journal2007，，35：	1
9	Expression of AQP-2 family in rat kidneys during development and maturation显示文摘	Yamamoto T Sei S Kiyohide F	1997	Am J Physiol1997，272，41：	1
10	Preparation and functional of fish gelatin-chitosan blend edible films显示文摘	Seyed F H Masoud R Mojgan Z Farhid F G	2013	Food chemistry2013，136，34：	1
11	Review of flexible riser modelling and analysis techniques显示文摘	PATEL M H SEYED F B	1995	Eng Structures1995，17，4：	1
12	Pain management for total knee arthroplasty:single-injection femoral nerve block versus local infiltration analgesia显示文摘	Mehdi M Hossein F Seyed HR	2014	Iran Red Crescent Med J2014，，13：	1
13	Separation of L-lysine from di- lute aqueous solution using molecular imprinting technique显示文摘	REZA P EBRAHIM V F SEYED A S	2007	Biochem Eng J2007，35，：	1
14	A study of large break LOCA in the AP1000 reactor containment显示文摘	Rahim F C Mohammad Rahgoshay Seyed Khalil Mousavian	2012	Progress in Nuclear Energy2012，54，1：	1
15	Segmen- tation of point clouds using smoothness constraint显示文摘	RABBANI T VAN DEN ~ F V(3~SEI~ANN G	2006	Interna- tional Archives of Photogrammetry Remote Sensing and Spatial Information Sciences2006，36，5：	1
16	Development and Validation of LC - MS/MS Method for the Determination of Cyproheptadine in Several Pharmaceutical Syrup Formulations 显示文摘	Xesfis F Lei Y Seyed V H	2009	Journal of Pharmaceutical and Biomedical Analysis2009，50，5：	1
17	Review of flexible riser modelling and analysis techniques显示文摘	Patel M H Seyed F B	1995	Engineering Structures1995，17，4：	1
18	On the relationship between stock returns and exchange rates:tests of Granger causality显示文摘	RICHARD A A JOSEPH F SEYED M M	1998	Global finance journal1998，9，2：	1
19	Assessment of shi- ga Toxin-Producing Escherichia coli O157 illnesses pre- vented by recalls of beef products显示文摘	Seys S A Sampedro F Hedberg C W	2015	Foodborne Patho- gens and Disease2015，12，9：	1
20	An improvement in maximum likelihood location estimation algorithm In sensor networks 显示文摘	ZEINAB A AHMAD F HAMID HAJ SEYED J	2011	International Journal of Computer Science and Engineering2011，，：	1

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