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| 1 | Novel DNA vaccine based on hepatitis B virus core gene induces specific immune responses in Balb/c mice显示文摘AIM: To investigate the immunogenicity of a novel DNA vaccine,pSW3891/HBc, based on HBV core gene in Balb/c mice.METHODS: A novel DNA vaccine, pSW3891/HBc, encoding HBV core gene was constructed using a vector plasmid pSW3891. Balb/c mice were immunized with either pSW3891/HBc or empty vector DNA via gene gun. IgG anti-HBc responses in mouse sera were demonstrated by ELISA. Specific cytotoxicity of cytotoxic T lymphocytes (CTLs) of mice was quantitatively measured by lactate dehydrogenase release assay.RESULTS: HBcAg was expressed effectively in 293T cell line transiently transfected with pSW3891/HBc. Strong IgG anti-HBc responses were elicited in mice immunized with pSW3891/HBc. The end-point titers of anti-HBc reached the highest 1:97 200, 4 wk after the third immunization. The specific CTL killing with the highest specific lysis reached 73.25% at effector:target ratio of 20:1 in mice that received pSW3891/HBc DNA vaccine.CONCLUSION: pSW3891/HBc vaccination elicits specific anti-HBc response and induces HBc-specific CTL response in immunized Balb/c mice. | Yi-Ping Xing Zu-Hu Huang Shi-Xia Wang Jie Cai Jun Li Te-Hui W Chou Shan Lu | 2005 | World Journal of Gastroenterology2005，11，29： | 7 |
| 2 | Magnetic Structure of Archean Kongling Group from Yangtze Craton,South China显示文摘ThepaperissupportedbyNationalNaturalScienceFoundation,NationalEducationCommiteeandMinistryofGeologyandMineralResourcesofChina... | Liu Qingsheng Department of Applied Geophysics, China University of Geosciences, Wuhan 430074 Gao Shan Faculty of Earth Sciences, China University of Geosciences, Wuhan 430074 Xu Qidong Faculty of Earth Resources, China University of Geosciences, W | 1998 | Journal of Earth Science1998，17，2： | 5 |
| 3 | Iron increases HMOX1 and decreases hepatitis C viral expression in HCV-expressing cells显示文摘AIM:To investigate effects of iron on oxidative stress, heme oxygenase-1(HMOX1)and hepatitis C viral(HCV) expression in human hepatoma cells stably expressing HCV proteins. METHODS:Effects of iron on oxidative stress,HMOX1, and HCV expression were assessed in CON1 cells. Measurements included mRNA by quantitative reverse transcription-polymerase chain reaction,and protein levels by Western blots. RESULTS:Iron,in the form of ferric nitrilotriacetate,increased oxidative stress and up-regulated HMOX1 gene expression.Iron did not affect mRNA or protein levels of Bach1,a repressor of HMOX1.Silencing the up-regulation of HMOX1 nuclear factor-erythroid 2-related factor 2(Nrf2)by Nrf2-siRNA decreased FeNTA-mediated up-regulation of HMOX1 mRNA levels.These iron effects were completely blocked by deferoxamine(DFO).Iron also significantly decreased levels of HCV core mRNA and protein by 80%-90%, nonstructural 5A mRNA by 90%and protein by about 50%in the Con1 full length HCV replicon cells, whereas DFO increased them. CONCLUSION:Excess iron up-regulates HMOX1 and down-regulates HCV gene expression in hepatoma cells.This probably mitigates liver injury caused by combined iron overload and HCV infection. | Wei-Hong Hou Lisa Rossi Ying Shan Jian-Yu Zheng Richard W Lambrecht Herbert L Bonkovsky | 2009 | World Journal of Gastroenterology2009，15，36： | 5 |
| 4 | Effect of coronary bifurcation angle on clinical outcomes in Chinese patients treated with crush stenting: a subgroup analysis from DKCRUSH-1 bifurcation study显示文摘背景分叉角度可以影响压碎 stenting 的临床的结果。我们寻求了比较高度(60 ( | CHEN Shao-liang ZHANG Jun-jie YE Fei CHEN Yun-dai FANG Wei-yi WEI Meng HE Ben SUN Xue-wen YANG Song CHEN Jin-guo SHAN Shou-jie TIAN Nai-liang LI Xiao-bo LIU Zhi-zhong KAN Jing Lee Michael Kwan Tak W | 2009 | Chinese Medical Journal2009，，4： | 5 |
| 5 | Establishment and Applications of Epstein-Barr Virus-based Episomal Vectors in Human Embryonic Stem Cells显示文摘 | Ren C Zhao M Yang X Li D Jiang X Wang L Shan W Yang H Zhou L Zhou W Zhang H | 2006 | 中国生物学文摘2006，20，7： | 4 |
| 6 | High corrosion resistance of electroless composite plating coatings on AZ91D magnesium alloys 显示文摘 | SONG Y W SHAN D Y CHEN R S HAN E H | 2008 | Electrochimica Acta2008，53，： | 1 |
| 7 | 显示文摘 | Gao D S Chen W Xu Q | 2000 | J Wuhan Univ Techol Mater Sci Ed2000，15，： | 1 |
| 8 | High corrosion resistance of electroless composite plating coatings on AZ91D magnesium alloys显示文摘 | SONG Y W SHAN D Y CHENG R S | 2008 | Electrochimica Acta2008，53，5： | 1 |
| 9 | Positive solutions for second-order m-point boundary value problems显示文摘 | Guo Y P Shan W R Ge W G | 2003 | J Comput ApplMath2003，151，2： | 1 |
| 10 | Intestinal absorption of forsythoside A in different compositions of Shuang- Huang-Lian 显示文摘 | ZHOU W DI L Q SHAN J J | 2011 | Fitoterapia2011，82，3： | 1 |
| 11 | Effect of iodine intake on thyroid disease in China显示文摘 | Teng W Shan Z Teng X | 2006 | N Engl J Med2006，354，： | 1 |
| 12 | A novel improved variable step-size incremental-resistance MPPT method for PV systems 显示文摘 | MEI Q SHAN M W LIU L Y | 2011 | IEEE Transactions on Industrial Electronics2011，58，6： | 1 |
| 13 | Study on precision forging technology for a complex-shaped light alloy forging 显示文摘 | SHAN D B XU W C LU Y | 2004 | J of Mat Proc Tech2004，151，13： | 1 |
| 14 | Vertex methods for computing functions on fuzzy variables显示文摘 | DONG W SHAN H C | 1987 | Fuzzy Sets and Systems1987，24，： | 1 |
| 15 | Frequencies of GSTM1,GSTT1,and GSTP1 polymorphisms in ethnic minority from Guizhou显示文摘 | Zhang W Shan KR Wu CX | 2007 | Zhonghua Yi Xue Yi Chuan Xue Za Zhi2007，24，5： | 1 |
| 16 | The first detectionof human parechovirus infections in China显示文摘 | Shan T L Guo W Cui L | 2009 | Jour-nal of Clinical Virology2009，45，4： | 1 |
| 17 | Effect of iodine intake on thy-roid diseases in China显示文摘 | Teng W Shan Z Teng X | 2006 | N Engl J Med2006，354，26： | 1 |
| 18 | The properties of one-dimensional spin-S(S≥1) antiferromagnetic Ising chain with single-ion anisotropy显示文摘 | Chen X Y Jiang Q Shan W Z | 2003 | J Magn Magn Mater2003，262，： | 1 |
| 19 | Numerical simulation of droplet breakup and collision in the solution precursor plasma spraying 显示文摘 | Shan Y Coyle T W Mostaqhimi J | 2007 | Journal of Thermal Spray Technology2007，16，5： | 1 |
| 20 | Cyclooxygenase-2 in cancer progress and perspective显示文摘 | ZHA Shan YEGNASUBRAMANIAN V NELSON W G | 2004 | Cancer Letters2004，215，1： | 1 |
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