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| 1 | Plant Prime Editors Enable Precise Gene Editing in Rice Cells显示文摘Genome editing is revolutionizing plant research and crop breeding.Sequence-specific nucleases(SSNs)such as zinc finger nuclease(ZFN)and TAL effector nuclease(TALEN)have been used to create site-specific DNA double-strand breaks and to achieve precise DNA modifications by promoting homology-directed repair(HDR)(Steinert et al.,2016;Voytas,2013).Later,RNA-guided SSNs such as CRISPR-Cas9,Cas12a,Cas12b,and their variants were applied for genome editing in plants(Li et al.,2013;Nekrasov et alM 2013;Tang et al.,2017;Zhong et al.,2019;Ming et al.,2020;Tang et al.,2019).However,HDR relies on simultaneous delivery of SSNs and DNA donors,which has been challenging in plants(Steinert et al.,2016;Zhang et aL,2019).Another challenge for realizing efficient HDR in plants is that DNA repair favors nonhomologous end joining(NHEJ)pathways over HDR in most cell types(Puchta,2005;Qi et al.,2013). | Xu Tang Simon Sretenovic Qiurong Ren Xinyu Jia Mengke Li Tingting Fan Desuo Yin Shuyue Xiang Yachong Guo Li Liu Xuelian Zheng Yiping Qi Yong Zhang | 2020 | Molecular Plant2020，13，5： | 20 |
| 2 | 利用红外相机研究卧龙国家级自然保护区绿尾虹雉的活动规律显示文摘绿尾虹雉Lophophorus lhuysii是一种仅分布在中国西部山区的珍稀雉类。由于缺乏监测研究,目前关于绿尾虹雉行为与栖息地的一手资料较少。2013年11月—2016年3月,在四川卧龙国家级自然保护区高山带布设20台红外相机,总相机工作日7 056 d,拍摄记录约12万条。其中,绿尾虹雉有效探测106次,相对多度指数15.02。统计结果显示,绿尾虹雉年活动高峰是7月;日活动高峰是08∶00—10∶00和18∶00—20∶00,日活动节律存在季节和性别差异。绿尾虹雉活动最适宜的环境温度在0~10℃,有集群和季节性垂直迁移现象,目前在保护区生存状况较好。雄性绿尾虹雉的活动强度约为雌性的2倍。本研究有助于全面了解绿尾虹雉的生态习性,为进一步保护和研究绿尾虹雉提供了基础信息。 | 唐卓 杨建 刘雪华 王鹏彦 李周园 LIU Cheng Simon | 2017 | 四川动物2017，36，5： | 18 |
| 3 | Minimally invasive surgery for gastric cancer: A comparison between robotic, laparoscopic and open surgery显示文摘AIM To investigate the role of minimally invasive surgery for gastric cancer and determine surgical, clinical, and oncological outcomes.METHODS This is a propensity score-matched case-control study, comparing three treatment arms: robotic gastrectomy(RG), laparoscopic gastrectomy(LG), open gastrectomy(OG). Data collection started after sharing a specific study protocol. Data were recorded through a tailored and protected web-based system. Primary outcomes: harvested lymph nodes, estimated blood loss, hospital stay, complications rate. Among the secondary outcomes, there are: operative time, R0 resections, POD of mobilization, POD of starting liquid diet and soft solid diet. The analysis includes the evaluation of type and grade of postoperative complications. Detailed information of anastomotic leakages is also provided.RESULTS The present analysis was carried out of 1026 gastrectomies. To guarantee homogenous distribution of cases, patients in the RG, LG and OG groups were 1:1:2 matched using a propensity score analysis with a caliper = 0.2. The successful matching resulted in a total sample of 604 patients(RG = 151; LG = 151; OG = 302). The three groups showed no differences in all baseline patients characteristics, type of surgery(P = 0.42) and stage of the disease(P = 0.16). Intraoperative blood loss was significantly lower in the LG(95.93 ± 119.22) and RG(117.91 ± 68.11) groups compared to the OG(127.26 ± 79.50, P = 0.002). The mean number of retrieved lymph nodes was similar between the RG(27.78 ± 11.45), LG(24.58 ± 13.56) and OG(25.82 ± 12.07) approach. A benefit in favor of the minimally invasive approaches was found in the length of hospital stay(P < 0.0001). A similar complications rate was found(P = 0.13). The leakage rate was not different(P = 0.78) between groups.CONCLUSION Laparoscopic and robotic surgery can be safely performed and proposed as possible alternative to open surgery. The main highlighted benefit is a faster postoperative functional recovery. | Amilcare Parisi Daniel Reim Felice Borghi Ninh T Nguyen Feng Qi Andrea Coratti Fabio Cianchi Maurizio Cesari Francesca Bazzocchi Orhan Alimoglu Johan Gagnière Graziano Pernazza Simone D'Imporzano Yan-Bing Zhou Juan-Santiago Azagra Olivier Facy Steven T Brower Zhi-Wei Jiang Lu Zang Arda Isik Alessandro Gemini Stefano Trastulli Alexander Novotny Alessandra Marano Tong Liu Mario Annecchiarico Benedetta Badii Giacomo Arcuri Andrea Avanzolini Metin Leblebici Denis Pezet Shou-Gen Cao Martine Goergen Shu Zhang Giorgio Palazzini Vito D'Andrea Jacopo Desiderio | 2017 | World Journal of Gastroenterology2017，23，13： | 17 |
| 4 | What Happened in the Trans-North China Orogen in the Period 2560-1850 Ma?显示文摘Trans 北方中国造山带(TNCO ) 是东方、西方的块沿着混合了形成一个协调 NorthChina 古地台(NCC ) 的一条 Paleoproterozic continent-continentcollisional 带。最近地质,结构,有在东方块的西方的边缘下面的海洋的岩石圈的东方指导俯冲的 geochemical 和 theorogen 沿着东方块的西方的边缘是大陆人边缘或日本类型弧的同位素的数据表演,由旧海洋与西方的块被分开,。在 2550-2520 妈, deepsubduction 引起了媒介降低的外壳的部分融化,生产被侵入进形成 granitoid plutons 在的外壳的上面的层次的丰富的 granitoid 岩浆低 --tomedium 等级花岗石绿岩岩层。在 2530-2520 妈,海洋的岩石圈的俯冲引起了披风楔的部分融化,它在弧在下地壳和普遍 mafic 和次要的 felsic 火山作用在 mafic 岩浆的 plating 下面导致了,形成绿岩集合的部分。普遍 mafic 驾驶到 felsic 火山作用的扩展在造山带导致了发展 ofback 弧或 intra 弧盆。在 2520-2475 妈,俯冲引起了形成大量 tonalitic-trondhjemitic-granodioritic (TTG ) 的下地壳的进一步偏爱的融化岩浆酌。背弧盆的在这时候后面的进一步的扩展,阵发性的 granitoidmagmatism 发生了,导致 2360 妈,的炮兵阵地~ 2 250 妈 2110-21760 妈和~ 2 在造山带的 050 Magranites。当代的火山沉积的岩石在背弧 orintra 弧盆发展了。在 2150-1920 妈,造山带经历了几前紧张的事件,一条海洋的山脉的可能到期的 tosubduction,导致是的 mafic 女同性恋的炮兵阵地随后变形了到角闪岩和媒介 -- 到高压的 mafic granulites。在 1880-1820 妈,在东方、西方的块之间的海洋被俯冲完全消费,并且关门海洋导致了 continent-arc-continent 碰撞,它引起了大规模推进和等斜褶皱并且搬运了一些岩石进更低的外壳的层次或上地幔形成 granulites 或 eclogites 。山峰变态被 exhumation/uplift 跟随,导致在岩石中的不对称褶皱和 symplectic 质地的普遍开发。 | Guochun ZHAO LIU Shuwen Min SUN LI Sanzhong Simon WILDE Xiaoping XIA Jian ZHANG Yanhong HE | 2006 | Acta Geologica Sinica(English Edition)2006，80，6： | 16 |
| 5 | Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG显示文摘Two recently engineered SpCas9 variants, namely xCas9 and Cas9-NG, show promising potential in improving targeting specificity and broadening the targeting range. In this study, we evaluated these Cas9 variants in the model and crop plant, rice. We first tested xCas9-3.7, the most effective xCas9 variant in mammalian cells, for targeted mutagenesis at 16 possible NGN PAM (protospacer adjacent motif) combinations in duplicates. xCas9 exhibited nearly equivalent editing efficiency to wild-type Cas9 (Cas9-WT) at most canonical NGG PAM sites tested, whereas it showed limited activity at non-canonical NGH (H = A, C, T) PAM sites. High editing efficiency of xCas9 at NGG PAMs was further demonstrated with C to T base editing by both rAPOBECI and PmCDAI cytidine deaminases. With mismatched sgRNAs, we found that xCas9 had improved targeting specificity over the Cas9-WT. Furthermore, we tested two Cas9-NG variants, Cas9-NGv1 and Cas9-NG, for targeting NGN PAMs. Both Cas9-NG variants showed higher editing efficiency at most non-canonical NG PAM sites tested, and enabled much more efficient editing than xCas9 at AT-rich PAM sites such as GAT, GAA, and CAA. Nevertheless, we found that Cas9-NG variants showed significant reduced activity at the canonical NGG PAM sites. In stable transgenic rice lines, we demonstrated that Cas9-NG had much higher editing efficiency than Cas9-NGv1 and xCas9 at NG PAM sites. To expand the base-editing scope, we developed an efficient C to T base-editing system by making fusion of Cas9-NG nickase (D10A version), PmCDAI, and UGI. Taken together, our work benchmarked xCas9 as a high-fidelity nuclease for targeting canonical NGG PAMs and Cas9-NG as a preferred variant for targeting relaxed PAMs for plant genome editing. | Zhaohui Zhong Simon Sretenovic Qiurong Ren Lijia Yang Yu Bao Caiyan Qi Mingzhu Yuan Yao He Shishi Liu Xiaopei Liu Jiaheng Wang Lan Huang Yan Wang Dibin Baby David Wang Tao Zhang Yiping Qi Yong Zhang | 2019 | Molecular Plant2019，12，7： | 13 |
| 6 | Global Wheat Head Detection(GWHD)Dataset:A Large and Diverse Dataset of High-Resolution RGB-Labelled Images to Develop and Benchmark Wheat Head Detection Methods显示文摘The detection of wheat heads in plant images is an important task for estimating pertinent wheat traits including head population density and head characteristics such as health,size,maturity stage,and the presence of awns.Several studies have developed methods for wheat head detection from high-resolution RGB imagery based on machine learning algorithms.However,these methods have generally been calibrated and validated on limited datasets.High variability in observational conditions,genotypic differences,development stages,and head orientation makes wheat head detection a challenge for computer vision.Further,possible blurring due to motion or wind and overlap between heads for dense populations make this task even more complex.Through a joint international collaborative effort,we have built a large,diverse,and well-labelled dataset of wheat images,called the Global Wheat Head Detection(GWHD)dataset.It contains 4700 high-resolution RGB images and 190000 labelled wheat heads collected from several countries around the world at different growth stages with a wide range of genotypes.Guidelines for image acquisition,associating minimum metadata to respect FAIR principles,and consistent head labelling methods are proposed when developing new head detection datasets.The GWHD dataset is publicly available at http://www.global-wheat.com/and aimed at developing and benchmarking methods for wheat head detection. | Etienne David Simon Madec Pouria Sadeghi-Tehran Helge Aasen Bangyou Zheng Shouyang Liu Norbert Kirchgessner Goro Ishikawa Koichi Nagasawa Minhajul A.Badhon Curtis Pozniak Benoit de Solan Andreas Hund Scott C.Chapman Frédéric Baret Ian Stavness Wei Guo | 2020 | Plant Phenomics2020，2，1： | 13 |
| 7 | Impact of diabetes mellitus on risk of cardiovascular disease and all-cause mortality: Evidence on health outcomes and antidiabetic treatment in United States adults显示文摘AIMTo examine the epidemic of diabetes mellitus (DM) and its impact on mortality from all-cause and cardiovascular disease (CVD), and to test the effect of antidiabetic therapy on the mortality in United States adults. METHODS The analysis included a randomized population sample of 272149 subjects ages ≥ 18 years who participated in the National Health Interview Surveys (NHIS) in2000-2009. Chronic conditions (hypertension, DM and CVD) were classified by participants’ self-reports of physician diagnosis. NHIS-Mortality Linked Files, and NHIS-Medical Expenditure Panel Survey Linkage Fileson prescribed medicines for patients with DM were used to test the research questions. χ 2, Poisson and Cox’s regression models were applied in data analysis.RESULTS Of all participants, 22305 (8.2%) had DM. The prevalence of DM significantly increased from 2000 to 2009 in all age groups (P < 0.001). Within an average 7.39 (SD= 3) years of follow-up, male DM patients had 1.56 times higher risk of death from all-cause (HR = 1.56, 95%CI:1.49-1.64), 1.72 times higher from heart disease [1.72 (1.53-1.93)], 1.48 times higher from cerebrovascular disease [1.48 (1.18-1.85)], and 1.67 times higher from CVD [1.67 (1.51-1.86)] than subjects without DM,respectively. Similar results were observed in females. In males, 10% of DM patients did not use any antidiabetic medications, 38.1% used antidiabetic monotherapy, and 51.9% used ≥ 2 antidiabetic medications. These corresponding values were 10.3%, 40.4% and 49.4% in females. A significant protective effect of metformin monotherapy or combination therapy (except for insulin) on all-cause mortality and a protective but non-significant effect on CVD mortality were observed. CONCLUSION This is the first study using data from multiple linkage files to confirm a significant increased prevalence of DM in the last decade in the United States. Patients with DM have significantly higher risk of death from all-cause and CVD than those without DM. Antidiabetic mediations,specifically for metformin use, show a protective effect against all-cause and CVD mortalities. | Longjian Liu Barbara Simon Jinggaofu Shi Arshpreet Kaur Mallhi Howard J Eisen | 2016 | World Journal of Diabetes2016，7，18： | 12 |
| 8 | Immune checkpoint inhibitor-related hepatotoxicity:A review显示文摘The application of immune checkpoint inhibitors(ICI)in advanced cancer has been a major development in the last decade.The indications for ICIs are constantly expanding into new territory across different cancers,disease stages and lines of therapy.With this increased use,adverse events including immune checkpoint inhibitor-related hepatotoxicity(ICH)have emerged as an important clinical problem.This along with the introduction of ICI as first-and second-line treatments for advanced hepatocellular carcinoma makes ICH very relevant to gastroenterologists and hepatologists.The incidence of ICH varies between 1%-20%depending on the number,type and dose of ICI received.Investigation and management generally involve excluding differential diagnoses and following a stepwise escalation of withholding or ceasing ICI,corticosteroid treatment and adding other immunosuppressive agents depending on the severity of toxicity.The majority of patients with ICH recover and some may even safely recommence ICI therapy.Guideline recommendations are largely based on evidence derived from retrospective case series which highlights a priority for future research. | Devika Remash David S Prince Catriona McKenzie Simone I Strasser Steven Kao Ken Liu | 2021 | World Journal of Gastroenterology2021，27，32： | 9 |
| 9 | Crosstalk Complexities between Auxin, Cytokinin, and Ethylene in Arabidopsis Root Development: From Experiments to Systems Modeling, and Back Again显示文摘理解荷尔蒙和基因怎么交往在变化环境协调植物生长是在植物的主要挑战发展生物学。植物生长素,细胞激动素,和乙烯是调整植物开发的许多方面的三重要荷尔蒙。这评论极其在 Arabidopsis 根开发评估在三荷尔蒙之间的串音。我们把许多试验性的数据集成到一个串音网络,它在植物生长素,细胞激动素,和乙烯串音揭示复杂性的多重层。特别地,数据集成揭示在乙烯和细胞激动素小径之间的一个另外的、大部分俯看的连接,它通过 phosphorelay 机制行动。这个建议连接探讨乙烯申请是否支持或禁止乙烯受体的受体 kinase 活动的突出的问题。阐明在植物生长素,细胞激动素,和乙烯串音的复杂性要求一联合试验性并且系统建模途径。我们为证实在植物生长素,细胞激动素,和乙烯之间的串音怎么在根开发调整 patterning 评估重要当模特儿的努力。我们讨论反复地把实验与为分析建模的系统相结合的新奇方法论怎么为阐明是必要的在在根开发的植物生长素,细胞激动素,和乙烯的串音的复杂性。最后,我们讨论未来挑战从一联合试验性、建模的观点。 | Junli Liu Simon Moore Chunli Chen Keith Lindsey | 2017 | Molecular Plant2017，10，12： | 8 |
| 10 | Corrosion of magnesium and magnesium–calcium alloy in biologically-simulated environment显示文摘A study of biocompatibility and corrosion of both metallic magnesium(Mg) and a magnesium alloy containing 1% calcium(Mg–Ca) were investigated in in vitro culture conditions with and without the presence of bone marrow derived human mesenchymal stem cells(h MSCs).Chemical analysis of the degraded samples was performed using XRD and FEGSEM. The results from the XRD analysis strongly suggested that crystalline phase of magnesium carbonate was present on the surface of both the Mg and Mg–Ca samples. Flame absorption spectrometry was used to analyse the release of magnesium and calcium ions into the cell culture medium. Magnesium concentration was kept consistently at a level ranging from 40 to 80 m M for both Mg and Mg–Ca samples. No cell growth was observed when in direct contact with the metals apart from a few cells observed at the bottom of culture plate containing Mg–Ca alloy. In general, in vitro study of corrosion of Mg–Ca in a biologicallysimulated environment using cell culture medium with the presence of h MSCs demonstrated close resemblances to in vivo corrosion. Although in vitro corrosion of Mg–Ca revealed slow corrosion rate and no immediate cytotoxicity effects to h MSCs, its corrosion rate was still too high to achieve normal stem cell growth when cells and alloys were cultured in vitro in direct contact. | Richard Harrison Diana Maradze Simon Lyons Yufeng Zheng Yang Liu | 2014 | Progress in Natural Science:Materials International2014，24，5： | 8 |
| 11 | Genomic Analyses Yield Markers for Identifying Agronomically Important Genes in Potato显示文摘野土豆种类有实质的 phenotypic 和生理的差异。这里,我们基于茄属节 Petota 的 201 就职的 genomic 分析报导对野、栽培的土豆种类的一个全面评价。我们定序这 201 就职的染色体并且识别了 6 ? 487 ? 从在 clade 的 167 就职的 006 高质量的单个核苷酸多型性(SNP ) 4 茄属节 Petota,包括 146 野并且有宽广地理分布的 21 栽培双土豆就职。染色体宽的基因变化分析比栽培土豆,和在农学地重要的疾病抵抗的高得多的基因差异,基因在野土豆被观察的证明野土豆的差异高。由关于已知的量的特点 loci (QTL ) 利用信息,而且,我们在选择下面识别了 609 基因,包括那些在 tubers 与痛苦的损失相关,那些在 tuberization 包含了,土豆的二个主要驯养的特点。种系发生的分析在 clade 揭示了所有种类的一个纵贯的部门 4,不是就那些在 S.? brevicaule 建筑群,和进一步支持的 S。是的 candolleanum 栽培土豆和在南部的秘鲁的栽培土豆的 monophyletic 起源的祖先。另外,我们分析了 S. 的染色体 ? candolleanum 并且鉴别 529 基因在栽培土豆输了。一起,在这研究产生的分子的标记为为土豆繁殖有用的农学地重要的基因的鉴定提供一个珍贵资源。 | Yangping Li Christophe Colleoni Junjie Zhang Qiqi Liang Yufeng Hu Holly Ruess Reinhard Simon Yinghong Liu Hanmei Liu Guowu Yu Eric Schmitt Ghloe Ponitzki Guangjian Liu Huanhuan Huang Feilong Zhan Lin Chen Yubi Huang David Spooner Binquan Huang | 2018 | Molecular Plant2018，11，3： | 8 |
| 12 | Biology,invasion and management of the agricultural invader:Fall armyworm,Spodoptera frugiperda(Lepidoptera:Noctuidae)显示文摘The fall armyworm(FAW),Spodoptera frugiperda(J.E.Smith),is native to the Americas.It has rapidly invaded 47 African countries and 18 Asian countries since the first detection of invasion into Nigeria and Ghana in 2016.It is regarded as a super pest based on its host range(at least 353 host plants),its inherent ability to survive in a wide range of habitats,its strong migration ability,high fecundity,rapid development of resistance to insecticides/viruses and its gluttonous characteristics.The inherently superior biological characteristics of FAW contribute to its invasiveness.Integrated pest management(IPM)of FAW has relied on multiple applications of monitoring and scouting,agricultural control,chemical pesticides,viral insecticides,sex attractants,bio-control agents(parasitoids,predators and entomopathogens)and botanicals.Knowledge gaps remain to be filled to:(1)understand the invasive mechanisms of S.frugiperda;(2)understand how to prevent its further spread and(3)provide better management strategies.This review summarizes the biological characters of FAW,their association with its invasiveness and IPM strategies,which may provide further insights for future management. | Jing WAN HUANG Cong LI Chang-you ZHOU Hong-xu REN Yong-lin LI Zai-yuan XING Long-sheng ZHANG Bin QIAO Xi LIU Bo LIU Cong-hui XI Yu LIU Wan-xue WANG Wen-kai QIAN Wan-qiang Simon MCKIRDY WAN Fang-hao | 2021 | Journal of Integrative Agriculture2021，20，3： | 7 |
| 13 | 863 genomes reveal the origin and domestication of chicken显示文摘Despite the substantial role that chickens have played in human societies across the world,both the geographic and temporal origins of their domestication remain controversial.To address this issue,we analyzed 863 genomes from a worldwide sampling of chickens and representatives of all four species of wild jungle fowl and each of the five subspecies of red jungle fowl(RJF).Our study suggests that domestic chickens were initially derived from the RJF subspecies Gallus gallus spadiceus whose present-day distribution is predominantly in southwestern China,northern Thailand and Myanmar.Following their domestication,chickens were translocated across Southeast and South Asia where they interbred locally with both RJF subspecies and other jungle fowl species.In addition,our results show that the White Leghorn chicken breed possesses a mosaic of divergent ancestries inherited from other subspecies of RJF.Despite the strong episodic gene flow from geographically divergent lineages of jungle fowls,our analyses show that domestic chickens undergo genetic adaptations that underlie their unique behavioral,morphological and reproductive traits.Our study provides novel insights into the evolutionary history of domestic chickens and a valuable resource to facilitate ongoing genetic and functional investigations of the world's most numerous domestic animal. | Ming-Shan Wang Mukesh Thakur Min-Sheng Peng Yu Jiang Laurent Alain Francois Frantz Ming Li Jin-Jin Zhang Sheng Wang Joris Peters Newton Otieno Otecko Chatmongkon Suwannapoom Xing Guo Zhu-Qing Zheng Ali Esmailizadeh Nalini Yasoda Hirimuthugoda Hidayat Ashari Sri Suladari Moch Syamsul Arifin Zein Szilvia Kusza Saeed Sohrabi Hamed Kharrati-Koopaee Quan-Kuan Shen Lin Zeng Min-Min Yang Ya-Jiang Wu Xing-Yan Yang Xue-Mei Lu Xin-Zheng Jia Qing-Hua Nie Susan Joy Lamont Emiliano Lasagna Simone Ceccobelli Humpita Gamaralalage Thilini Nisanka Gunwardana Thilina Madusanka Senasige Shao-Hong Feng Jing-Fang Si Hao Zhang Jie-Qiong Jin Ming-Li Li Yan-Hu Liu Hong-Man Chen Cheng Ma Shan-Shan Dai Abul Kashem Fazlul Haque Bhuiyan Muhammad Sajjad Khan Gamamada Liyanage Lalanie Pradeepa Silva Thi-Thuy Le Okeyo Ally Mwai Mohamed Nawaz Mohamed Ibrahim Megan Supple Beth Shapiro Olivier Hanotte Guojie Zhang Greger Larson Jian-Lin Han Dong-Dong Wu Ya-Ping Zhang | 2020 | Cell Research2020，30，8： | 7 |
| 14 | An App knock-in rat model for Alzheimer’s disease exhibiting Aβand tau pathologies,neuronal death and cognitive impairments显示文摘A major obstacle in Alzheimer’s disease(AD)research is the lack of predictive and translatable animal models that reflect disease progression and drug efficacy.Transgenic mice overexpressing amyloid precursor protein(App)gene manifest non-physiological and ectopic expression of APP and its fragments in the brain,which is not observed in AD patients.The App knock-in mice circumvented some of these problems,but they do not exhibit tau pathology and neuronal death.We have generated a rat model,with three familiar App mutations and humanized Aβsequence knocked into the rat App gene.Without altering the levels of full-length APP and other APP fragments,this model exhibits pathologies and disease progression resembling those in human patients:deposit of Aβplaques in relevant brain regions,microglia activation and gliosis,progressive synaptic degeneration and AD-relevant cognitive deficits.Interestingly,we have observed tau pathology,neuronal apoptosis and necroptosis and brain atrophy,phenotypes rarely seen in other APP models.This App knock-in rat model may serve as a useful tool for AD research,identifying new drug targets and biomarkers,and testing therapeutics. | Keliang Pang Richeng Jiang Wei Zhang Zhengyi Yang Lin-Lin Li Makoto Shimozawa Simone Tambaro Johanna Mayer Baogui Zhang Man Li Jiesi Wang Hang Liu Ailing Yang Xi Chen Jiazheng Liu Bengt Winblad Hua Han Tianzi Jiang Weiwen Wang Per Nilsson Wei Guo Bai Lu | 2022 | Cell Research2022，32，2： | 6 |
| 15 | Inferring the Tree of Life of the order Cypriniformes, the earth's most diverse clade of freshwater fishes: Implications of varied taxon and character sampling显示文摘The phylogenetic relationships of species are fundamental to any biological investigation, including all evolutionary studies. Accurate inferences of sister group relationships provide the researcher with an historical framework within which the attributes or geographic origin of species (or supraspecific groups) evolved. Taken out of this phylogenetic context, interpretations of evolutionary processes or origins, geographic distributions, or speciation rates and mechanisms, are subject to nothing less than a biological experiment without controls. Cypriniformes is the most diverse clade of freshwater fishes with estimates of diversity of nearly 3,500 species. These fishes display an amazing array of morphological, ecological, behavioral, and geographic diversity and offer a tremendous opportunity to enhance our understanding of the biotic and abiotic factors associated with diversification and adaptation to environments. Given the nearly global distribution of these fishes, they serve as an important model group for a plethora of biological investigations, including indicator species for future cli-matic changes. The occurrence of the zebrafish, Danio rerio, in this order makes this clade a critical component in understanding and predicting the relationship between mutagenesis and phenotypic expressions in vertebrates, including humans. With the tremendous diversity in Cypriniformes, our understanding of their phylogenetic relationships has not proceeded at an acceptable rate, despite a plethora of morphological and more recent mo-lecular studies. Most studies are pre-Hennigian in origin or include relatively small numbers of taxa. Given that analyses of small numbers of taxa for molecular characters can be compromised by peculiarities of long-branch attraction and nodal-density effect, it is critical that significant progress in our understanding of the relationships of these important fishes occurs with increasing sampling of species to mitigate these potential problems. The recent Cypriniformes Tree of Life initiative is an effort to achieve this goal with morphological and molecular (mitochondrial and nuclear) data. In this early synthesis of our understanding of the phylogenetic relationships of these fishes, all types of data have contributed historically to improving our understanding, but not all analyses are complementary in taxon sampling, thus precluding direct understanding of the impact of taxon sampling on achieving accurate phylogenetic inferences. However, recent molecular studies do provide some insight and in some instances taxon sampling can be implicated as a variable that can influence sister group relationships. Other instances may also exist but without inclusion of more taxa for both mitochondrial and nuclear genes, one cannot distinguish between inferences being dictated by taxon sampling or the origins of the molecular data. | Richard L. MAYDEN Kevin L. TANG Robert M. WOOD Wei-Jen CHEN Mary K. AGNEW Kevin W. CONWAY Lei YANG Andrew M. SIMONS Henry L. BART Phillip M. HARRIS Junbing LI Xuzhen WANG Kenji SAITOH Shunping HE Huanzhang LIU Yiyu CHEN Mutstmi NISHIDA Masaki MIYA | 2008 | 植物分类学报2008，46，3： | 5 |
| 16 | The anti-obesity effects of EGCG in relation to oxidative stress and air-pollution in China显示文摘Modern China,similar to most developing nations,has seen a rise in the prevalence of both obesity and diesel exhaust based air pollution.The cause of obesity is multi-factorial encompassing diet,lifestyle and social factors.Also there has been a reduction in the consumption of fruit,vegetables,and traditional medicinal foods such as polyphenol containing green tea.Replacing these,are high fat and carbohydrate based processed foods which are quickly displacing these wholefoods in the diet.This review paper proposes evidence that a potential cause of obesity is also linked to environmental stress stimuli such as air pollutants,particularly diesel exhaust fumes(DEF)of>2.5μm particulate matter,and discusses a role for a green tea catechin(EGCG)for use as a dietary defence against diet and environmentally induced obesity.China is now at a critical point of a public health pandemic with rising air-borne pollution(via car exhaust fumes DEF),industry pollution such as heavy metals,and the benzene hydrocarbon based‘2PM’particulate matter,now accepted as a major environmental issue for public health.Relevant data published in MEDLINE since 1995 has been gathered to formulate the following review. | Simon CICHELLO Pingsheng LIU Markendya JOIS | 2013 | Natural Products and Bioprospecting2013，3，6： | 4 |
| 17 | An integrative Tai Chi program for patients with breast cancer undergoing cancer therapy:study protocol for a randomized controlled feasibility study显示文摘Background:Breast cancer patients experience various side effects during cancer therapy, often resulting in reduced quality of life and poor adherence to treatment. A limited range of proven interventions has been developed to target such side effects. While Tai Chi offers benefits for the health and well-being of breast cancer survivors, the effectiveness of Tai Chi across the treatment continuum has not been evaluated. Improved patient education and support has been suggested as a priority for breast cancer care. This pilot study assesses the feasibility of a randomized controlled trial(RCT) to evaluate the effectiveness of‘‘an integrative Tai Chi'(ANITA) program for breast cancer patients undergoing cancer therapy.Methods/design: This is a single-centre, two-arm feasibility RCT. Twenty-four patients with breast cancer who have undergone surgical treatment will be recruited from the Dunedin Hospital(New Zealand) over a 12-month period(from August 2017 to July 2018). Subject to informed consent, patients will be randomized to receive standard cancer treatment alone or standard cancer treatment plus the ANITA program, consisting of peer support, health education, and Tai Chi Ruler exercise. The program runs alongside the patient's adjuvant cancer therapy, which may include chemotherapy, radiation therapy,antibody treatment, and/or antihormonal therapy. Analysis in this study will focus on process evaluation of participant recruitment, retention, treatment fidelity, acceptability of the program, and occurrence of adverse events. Clinical outcomes(i.e., fatigue, sleep quality, anxiety and depression and quality of life)will be assessed at baseline, and at 12 weeks and 24 weeks post-randomization.Discussion: Outcomes from this study will inform the feasibility and methodology for a future fullypowered RCT. | Lizhou Liu Simone Petrich Blair McLaren Lyndell Kelly G.David Baxter | 2018 | Journal of Integrative Medicine2018，16，2： | 4 |
| 18 | Machine learning in orthopaedic surgery显示文摘Artificial intelligence and machine learning in orthopaedic surgery has gained mass interest over the last decade or so.In prior studies,researchers have demonstrated that machine learning in orthopaedics can be used for different applications such as fracture detection,bone tumor diagnosis,detecting hip implant mechanical loosening,and grading osteoarthritis.As time goes on,the utility of artificial intelligence and machine learning algorithms,such as deep learning,continues to grow and expand in orthopaedic surgery.The purpose of this review is to provide an understanding of the concepts of machine learning and a background of current and future orthopaedic applications of machine learning in risk assessment,outcomes assessment,imaging,and basic science fields.In most cases,machine learning has proven to be just as effective,if not more effective,than prior methods such as logistic regression in assessment and prediction.With the help of deep learning algorithms,such as artificial neural networks and convolutional neural networks,artificial intelligence in orthopaedics has been able to improve diagnostic accuracy and speed,flag the most critical and urgent patients for immediate attention,reduce the amount of human error,reduce the strain on medical professionals,and improve care.Because machine learning has shown diagnostic and prognostic uses in orthopaedic surgery,physicians should continue to research these techniques and be trained to use these methods effectively in order to improve orthopaedic treatment. | Simon P Lalehzarian Anirudh K Gowd Joseph N Liu | 2021 | World Journal of Orthopedics2021，12，9： | 4 |
| 19 | Multi-limb necrotizing fasciitis in a patient with rectal cancer显示文摘引起坏死筋膜炎是破坏软织物感染影响扁带和下的软纸巾。文学评论识别了几个相关风险因素,包括恶意,酒精中毒,营养不良,糖尿病,男性和老年。仅仅在与颜色描述它的稀罕协会的文学有贫乏的病案报告表面的恶意。所有出版的格被归因于在会阴的区域上导致引起坏死筋膜炎的肠穿孔。孤立上面或下肢疾病很少被识别。同时上面并且在颜色的下肢感染表面的癌症病人从来没在文学被描述过。我们与内在的非穿孔的直肠的癌在一个病人报导多手足引起坏死筋膜炎的一个不平常的案例。 | Shirley Yuk Wah Liu Simon Siu Man Ng Janet Fung Yee Lee | 2006 | World Journal of Gastroenterology2006，12，32： | 4 |
| 20 | Comparison of Leptosphaeria biglobosa-induced and chemically induced systemic resistance to L.maculans in Brassica napus显示文摘Brassica napus(cv.Madrigal)seedlings pre-treated with ascospores of Leptosphaeria biglobosa or foliar sprays of either acibenzolar-S-methyl(ASM)or menadione sodium bisulphite(MSB)were chal- lenge inoculated with L.maculans ascospores and assessed for phoma leaf spot development and tissue morphology and gene expression responses to infection.Rates of increase in phoma leaf spot area 8―21 d after challenge inoculation were significantly greater on water pre-treated plants than on plants pre-treated with L.biglobosa,ASM or MSB on both pre-treated leaves(local effect)and younger leaves without pre-treatment(systemic effect).Ninety-six h after challenge inoculation,the invasive hyphae of L.maculans were encircled by rings of necrotic mesophyll cells on leaves pre-treated with L. biglobosa,ASM or MSB but not those pre-treated with water.Quantification of transcript levels of genes commonly used as markers of the major defence signalling pathways(PDF1.2,PR-1,NPR1,APX, CHB4)0–96 h after L.maculans challenge inoculation showed expression patterns indicating prefer- ential activation of the jasmonate/ethylene pathway and involved induction of NPR1 locally and sys- temically in leaves of plants pre-treated with L.biglobosa ascospores. | LIU ShengYi LIU RenHu Akinwunmi O. LATUNDE-DADA Hans J. COOLS Simon J. FOSTER HUANG YongJu Bruce D. L. FITT | 2007 | Chinese Science Bulletin2007，52，8： | 4 |
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