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| 1 | 脂多糖对大鼠乳腺上皮细胞酪蛋白基因表达的影响显示文摘本试验旨在探讨脂多糖(LPS)对大鼠乳腺上皮细胞中αs1-酪蛋白(CSN1S1)、β-酪蛋白(CSN2)和乳清蛋白(α-LA)3种主要酪蛋白基因表达的影响。采用单因素试验设计,用实时荧光定量PCR(RT-PCR)法研究在催乳素诱导作用下,0、100、500、1000、5000和25000 ng/mL的LPS作用于HC11细胞3和24 h对酪蛋白基因(CSN1S1、CSN2和α-LA)、葡萄糖转运因子1(GLUT1)、葡萄糖转运因子8(GLUT8)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)基因表达的影响。结果表明:不同剂量、不同作用时间的LPS作用于HC11细胞,均显著抑制了CSN2基因的表达;作用3 h时,500~1000 ng/mL的LPS显著增加了α-LA基因的表达量(P<0.05),作用24 h时,所有剂量的LPS均显著抑制了α-LA基因的表达(P<0.05);500~25000 ng/mL的LPS作用3 h时和所有剂量的LPS作用24 h时均显著增加了CSN1S1基因的表达量(P<0.05);LPS显著增加了TNF-α基因的表达量(P<0.05);1000 ng/mL的LPS作用3 h时和100~25000 ng/mL的LPS作用24 h时,均显著增加了IL-6基因的表达量(P<0.05);500~25000 ng/mL的LPS作用3 h时显著增加了IL-1β基因的表达量(P<0.05);从时间效应来看,总体趋势是3种细胞因子(IL-6、IL-1β、TNF-α)在作用24 h时的基因表达量均高于作用3 h时。大于5000 ng/mL的LPS作用3 h时显著增加了GLUT1基因的表达量(P<0.05),所有剂量LPS均显著抑制了GLUT8基因的表达(P<0.05)。综上,在大鼠乳腺上皮细胞中,LPS对不同酪蛋白基因的表达有不同的影响,LPS能抑制CSN2和α-LA基因的表达,却能增强CSN1S1基因的表达。 | 田青 SPITZER A J 赵凤启 | 2020 | 动物营养学报2020，32，7： | 4 |
| 2 | Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4显示文摘 | Visintin A Mazzoni A Spitzer J A | 2001 | Proc Natl Acad Sci U S A2001，98，12： | 2 |
| 3 | Pathway engineering for production of aromatics in Escherichia coli:confirunation of stoichiometric analysis by independent modulation of aroG, tktA,and Ppe activities显示文摘 | Patnaik R Spitzer R G Liao J | 1995 | Biotechnol Bioeng1995，46，： | 1 |
| 4 | Toll-like receptorl inhibits Toll-like receptor 4 signaling in endothelis cells显示文摘 | Spitzer J H Visintin A Mazzoni A | 2002 | Eur J Immunol2002，32，4： | 1 |
| 5 | Measuring the quality of life of cancer patients:a concise QL-Index for use by physicians显示文摘 | Spitzer WO Dobson AJ Hall J | 1981 | J Chronic Dis1981，34，12： | 1 |
| 6 | The influence of storage conditions on flavour changes in human milk显示文摘 | SPITZER J DOUCET S BUETTNER A | | 0，，： | 1 |
| 7 | Antirhino/entero- viral vinylacetylene benzimidazoles : a study of their activity and oral plasma levels in mice显示文摘 | Tebbe M J Spitzer W A Victor F | 1997 | J Med Chem1997，40，24： | 1 |
| 8 | Development of the Tinnitus Handicap Inventory显示文摘 | NEWMAN C W JACOBSON G P SPITZER J B | 1996 | Arch Otolaryngol Head Neck Surg1996，122，： | 1 |
| 9 | Retirement withdrawals: An analysis of the benefits of periodic 'midcourse' adjustments 显示文摘 | Spitzer J J | 2008 | Financial Services Review2008，17，1： | 1 |
| 10 | Effectof oxide layers on spray water cooling heat transfer athigh surface temperature显示文摘 | Wendelstorf R Spitzer K H Wendelstorf J | 2008 | International Journal ofHeat and Mass Transer2008，51，1920： | 1 |
| 11 | Measuring the quality of life of cancer patients: a concise QL-tndex for use by physicians显示文摘 | Spitzer WO Dobson A J Hall J | 2008 | J Chronic Dis2008，34，12： | 1 |
| 12 | Application of remote sensing data to maping of shallow sea-floor near by Netherlands 显示文摘 | Mgengel V Spitzer R J | 1991 | International Journal of Remote Sensing1991，7，： | 1 |
| 13 | Warfarin anticoagulation exacerbates the risk of hemorrhagic trans-formation after rt - PA treatment in experimental stroke : therapeu- tic potential of PCC 显示文摘 | PFEILSCHIVFER W SPITZER D PFEILSCHIVTER J | 2011 | PLoS One2011，6，26： | 1 |
| 14 | Measuring the quality of life of cancer patients:a concise QL-index for use by physicians显示文摘 | Spitzer WO Dobson AJ Hall J | 2008 | J Chronic Dis2008，34，12： | 1 |
| 15 | Measuring the pualients:aconcise QL-index for use by physicians显示文摘 | Spitzer WO Dobson AJ Hall J | 1981 | J Chronic Dis1981，34，： | 1 |
| 16 | Reproductive responses and calf birth and weaning weights as affected by body condition at parturition and postpartum weight gain in primi- parous beef cows显示文摘 | Spitzer J C Morrison D G Wettemann R P et ai | 1995 | J Anim Sci1995，73，： | 1 |
| 17 | The visible human male: a technical report显示文摘 | Spitzer V Ackerman M J Scherizinger A L | 1996 | J Am Med Inform Assoc1996，3，2： | 1 |
| 18 | Bottom influence on the reflectance of the sea 显示文摘 | DIRKS R W J 1987 | 1987 | International Journal of Remote Sensing1987，8，3： | 1 |
| 19 | The effect of antirhino-and enteroviral vinylacetylene benzimidazoles of cytochrome P450 function and hepatic porphyr/n levels in mice显示文摘 | Tebbe M J Jensen C B Spitzer W A | 1999 | Antiviral Research1999，42，1： | 1 |
| 20 | Measuring the quality of life of cancer patients:a concise QL-index for use by physicians显示文摘 | SPITZER WO DOBON AJ HALL J | 1981 | Chronic Dis1981，34，12： | 1 |
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