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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Sib pair linkage and association studies between bone mineral density and the interleukin-6 gene locus显示文摘 | Takacs I Koller DL Peacock M | 2000 | Bone2000，27，1： | 1 |
| 3 | Electrophysiological effects of dronedarone ( SR 33589) , a noniodinated amiodarone derivative in the canine heart: comparison with amiodarone 显示文摘 | Varro A Takacs J Nemeth M | 2001 | Br J Pharma- col2001，133，5： | 1 |
| 4 | Sibling pair linkage and association studies between bone mineral density and the insulin-like growth factor I gene locus显示文摘 | Koller DL Peacock M | 1999 | J Clin Endocrinol Metab1999，84，12： | 1 |
| 5 | Effect of strong detergents and chaotropes on the detection of proteins in two-dimensional gels显示文摘 | Takacs B | 2001 | Electrophoresis2001，22，： | 1 |
| 6 | Increased circulating rapid peroxides in severe preeclampsia activate NF-(kappa)B and upregulate ICAM-1 in vascular endothelial cells显示文摘 | Takacs P Kauma S W Sholley M M | 2001 | FASEB J2001，15，： | 1 |
| 7 | The selective formation of neutral, heteroleptic zinc(Ⅱ ) complexes via self-discrimination of chiral bisoxazoline racemates and pseudoracemates显示文摘 | Takacs J M Hrvatin P M Atkins J M | 2005 | New J Chem2005，29，： | 1 |
| 8 | purification and characterization of recombinant human p50csk protein-tyrosine kinase from an Escherichia coli expression system overproducing the bacterial chaperones GroES and GroEL显示文摘 | Amrein KE Takacs B Stieger M | 1995 | Proc Natl Acad Sci USA1995，92，： | 1 |
| 9 | Asymmetric catalysis using self-assembled chiral bidentate P, P-ligands显示文摘 | Takacs J M Reddy D S Motekl S A | 2004 | J Am Chem Soc2004，126，： | 1 |
| 10 | Effect of strong detergents and chaotropes on the detection of proteins in two-dimensional gels 显示文摘 | FOUNTOULAKIS M TAKACS B | 2001 | Electrophoresis2001，22，9： | 1 |
| 11 | Activation of mouse and human peroxisome proliferator---Activated receptor alpha by perfluoroalkyl acids of different func- tional groups and chain lengths 显示文摘 | Wolf C J Takacs M L Schmid J E | 2008 | Toxicological Sci- ences2008，106，1： | 1 |
| 12 | Ultraviolet-visible and fluorescence spectral evidence of natural organic matter (NOM) changes along an estuarine salinity gradient 显示文摘 | Alberts J J Takacs M | 2004 | Organic Geochemistry2004，35，3： | 1 |
| 13 | Compressed histogram of gradients: A low-bitrate descriptor显示文摘 | CHANDRASEKHAR V TAKACS G CHEN D M | 2012 | International Journal of Computer Vision2012，96，3： | 1 |
| 14 | Epigenetic regulation of latent Epstein-Ban' virus promoters 显示文摘 | Takacs M Banati F Koroknai A | 2010 | Biochim Biophys Acta2010，1799，3： | 1 |
| 15 | Increased circulating lipid peroxides in severe preeclampsia activate NF-kappaB and upregulate ICAM-1 in vascular endothelial cells显示文摘 | Takacs P Kauma S Sholley M | 2001 | FASEB J2001，15，： | 1 |
| 16 | The first mechanochemist显示文摘 | Takacs L M Carey Lea | 2004 | J Mater Sci2004，39，16： | 1 |
| 17 | Resuscitation in the pediatric trauma population:admission base deficit remains an important prognostic indicator显示文摘 | Randolph LC Takacs M Davis KA | 2002 | J Trauma2002，53，5： | 1 |
| 18 | Multilevel Fuzzy Approach to the Risk and Disaster Man- agement显示文摘 | Takacs M | 2010 | Aeta Polyteehniea Hungarica2010，7，4： | 1 |
| 19 | Sibling pair linkage and association studies between bone mineral density and the insulin-like growth fa ctor 1 gene locus 显示文摘 | Takacs I Koller D L Peacock M | 1999 | J Clin Endocrinol Metab1999，84，： | 1 |
| 20 | Practical guidance for WWTP model calibration and associated data gather- ing requirements 显示文摘 | Dold P L Jones R M Takacs I | 2003 | Proc Water Environ Federation2003，2003，12： | 1 |
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