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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Construction and characterization of an experimental ISCOMS-based hepatitis B polypeptide vaccine显示文摘瞄准:描绘生物化学并且基于 HBsAg 的 T 房间 epitopes 从新奇治疗学的多肽准备的一支试验性的 ISCOMS 疫苗的免疫学的性质,和 heptatis B-ISCOMS 被准备并且调查。方法:刺激建筑群(ISCOMS ) 的免疫基于疫苗的包含新奇治疗学的肝炎 B 多肽被分离方法准备,并且它的形成被 SDS-polyacrylamide 胶化电气泳动化学上验证的电子显微镜学和简历设想。在 ISCOMS 以内的肽的数量被 Bradford 试金决定,并且特定的 CTL 反应被 ELISPOT 试金检测。结果:有大约 40nm 的一条直径的 submicroparticle 的典型像笼子的结构被电子显微镜学观察。从 Bradford 试金的结果证明肽加入的水平关于 0.33g .L (-1) 。在到分子的重量标记(3480kDa ) 的第六个乐队的 paralleled 位置结束,一个清楚的乐队在 SDS 页分析被给看,显示进 ISCOMS 的多肽的成功的加入。ISCOMS 交货系统能高效地改进多肽的 immunogenicity 并且由 ELISPOT 试金的结果在 vivo 得到特定的有免疫力的回答,这被建议,它证明生产房间(特定的 CTL 回答) 的 IFN-gamma 被增加(对待 ISCOMS 的组的点:47+/-5, n =3;控制组:5+/-2, n =3 ) 。结论:基于 ISCOMS 的肝炎 B 多肽疫苗成功地与在 vivo 疫苗的短多肽相比被构造,它导致更高的 CTL 回答。 | Xiao-Ju Guan Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 200031,China,previously worked as a postdoc in Institute of Immunology,Third Military Medical University,Chongqing 400038,China Xiao-Jun Guan,Department of Science & Research,Second Military Medical University,Shanghai 200433,China Yu-Zhang Wu Zheng-Cai Jia Tong-Dong Shi Yan Tan,Institute of Immunology,Third Military Medical University,Chongqing 400038,China | 2002 | World Journal of Gastroenterology2002，8，2： | 11 |
| 3 | Expression,purification and immunocharacteristics of recombination UreB protein of H.pylori显示文摘INTRODUCTIONHelicobacter pylori (H . pylori) is associated with the development of chronic gastritis ,peptic ulcer and gastric cancer and gastric MALT lymphoma[1-9],H .pylori has many antigens ,including urease ,heat shock protein and vacuolating cytotoxin and so on ,and urease is an important factor in the colinization of the gastric mucosa and suspected to cause damage to the gastric mucosa[10-14].At the same time ,urdase is also one of the important protective antigens . | Chao Wu~1 Quan Ming Zou~1 Hong Guo~2 Xiao Peng Yuan~1 Wei Jun Zhang~1 Dong Shui Lu~1 Xu Hu Mao~1 ~1Department of Clinical Microbiology,Third Military Medical University,Chongqing 400038,China ~2Department of Gastroenterology,Xinqiao Hospital,Third Military Medical University,Chongqing 40003?,ChinaDr.Chao Wu graduated from Third Military Medical University as a postgraduate in 2000,now a lecturer,specialized in diagnosis,prevention and therapy of Helicobacter pylori infection,having 6 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 5 |
| 4 | Long-term aeration management for improved N-removal via SND in a sequencing batch reactor显示文摘 | THIRD K A GIBBS B NEWLAND M | 2005 | Water Research2005，39，： | 1 |
| 5 | The role of iron-oxidizing bacterial in stimulation or inhibition of chalcopyrite bioleaching 显示文摘 | K A Third | 2000 | Hydrometallurgy2000，，57： | 1 |
| 6 | The CANON system (completely autotrophic nitrogen-removal over nitrite) under ammonium limitation: interaction and competition between three groups of bacteria 显示文摘 | Third K A Slickers A O Kuenen J G | 2001 | Systematic and Applied Microbiology2001，24，4： | 1 |
| 7 | CANON and anammox in a gas-lift reactor显示文摘 | K A Third | 2003 | FEMS Microbiology Letters2003，218，： | 1 |
| 8 | CANON and Anammox in a gas-lift reactor显示文摘 | Sliekers AO Third K A Abma W | 2003 | FEMS Microbiology Letters2003，218，2： | 1 |
| 9 | Long-term aeration management for improved N-removal via SND in a sequencing batch reactor显示文摘 | THIRD K A GIBBS B NEWLAND M | 2005 | Water Research2005，39，15： | 1 |
| 10 | The CANON system (completely autotrophic nitrogen-removal over nitrite) under ammonium limitation: Interaction and competition between three groups of bacteria显示文摘 | Third K A Sliekers A O Kuenen JG | 2001 | Systematic and Applied Microbiology2001，24，4： | 1 |
| 11 | The effect of dissolved oxygen on PHB accumulation in activated sludge cultures 显示文摘 | Katie A Third Mark Newland Ralf Cord-Ruwisch | 2003 | Biotechnology and Bioengineering2003，82，2： | 1 |
| 12 | Simultaneous nitrification and denitrification using stored substratc (PHB)as the electron donor in an SBR 显示文摘 | Katie A Third Natalie Burner Ralf Cord-Ruwisch | 2003 | Biotechnol Bioeng2003，83，6： | 1 |
| 13 | The presence of ammonium facilitates nitrite reduction under PHB driven simultaneous nitrification and denitrification显示文摘 | GIBBS B M SHEPHARD L R THIRD K A | 2004 | Water Science and Technology2004，50，10： | 1 |
| 14 | The CANON system (completely autotrophic nitrogen-removal over nitrite ) under ammonium limitation: Interaction and competition between three groups of bacteria 显示文摘 | Third K A Sliekers A O Kuenen J G | 2001 | Systematic and Applied Microbiology2001，24，4： | 1 |
| 15 | The effect of dissolved oxygen on PHB accumulation in activated sludge cultures 显示文摘 | Third K A Newland M Cord-Ruwisch R | 2003 | Biotechnol Bioeng2003，82，2： | 1 |
| 16 | CANON and anammox in a gas-lift, reactor 显示文摘 | Sliekers A O Third K A Abma W | 2003 | FEMS Microbiology Letters2003，218，2： | 1 |
| 17 | CANON and Anammox in a gas-lift reactor显示文摘 | SLIEKERS A O THIRD K ABMA W | 2003 | FEMS Microbiology Letters2003，218，2： | 1 |
| 18 | Simultaneous nitrification and denitri- fication using stored substrate (PHB) as the electron donor in an SBR显示文摘 | Third K A Burnett N Cord-Ruwisch R | 2003 | Biotechnology & Bioengineering2003，83，6： | 1 |
| 19 | Simultaneous nitrification and denitrification using stored substrate (PHB) as the electron doner in an SBR显示文摘 | Third K A Bumett N Ruwisch R C | 2003 | Biotechnol Bioeng2003，83，6： | 1 |
| 20 | CANON and ANAMMOX in a gas-lift reactor 显示文摘 | SLIEKERS A O THIRD K A ABMA W | 2003 | FEMS Microbiology Letters2003，218，2： | 1 |
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