|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | mi R-122 negatively correlates with liver fibrosis as detected by histology and FibroScan显示文摘AIM: To investigate whether expression of selected mi RNAs obtained from fibrotic liver biopsies correlate with fibrosis stage.METHODS: Altogether, 52 patients were enrolled in the study representing various etiologic backgrounds of fibrosis: 24 cases with chronic hepatitis infections(types B, C), 19 with autoimmune liver diseases(autoimmune hepatitis, primary biliary cirrhosis, primary sclerosing cholangitis, overlapping syndrome cases), and 9 of mixed etiology(alcoholic and nonalcoholic steatosis, cryptogenic cases). Severity of fibrosis was determined by both histologic staging using the METAVIR scoring system and noninvasive transient elastography. Following RNAisolation, expression levels of mi R-21, mi R-122, mi R-214, mi R-221, mi R-222, and mi R-224 were determined using Taq Man Micro RNA Assays applying mi R-140 as the reference. Selection of mi RNAs was based on their characteristic up- or downregulation observed in hepatocellular carcinoma. Relative expression of mi RNAs was correlated with fibrosis stage and liver stiffness(LS) value measured by transient elastography, as well as with serum alanine aminotransferase(ALT) level.RESULTS: The expression of individual mi RNAs showed deregulated patterns in stages F1-F4 as compared with stage F0, but only the reduced level of mi R-122 in stage F4 was statistically significant(P < 0.04). When analyzing mi RNA expression in relation to fibrosis, levels of mi R-122 and mi R-221 showed negative correlations with fibrosis stage, and mi R-122 was found to correlate negatively and mi R-224 positively with LS values(all P < 0.05). ALT levels displayed a positive correlation with mi R-21(P < 0.04). Negative correlations were observed in the fibrosis samples of mixed etiology between mi R-122 and fibrosis stage and LS values(P < 0.05), and in the samples of chronic viral hepatitis, between mi R-221 and fibrosis stage(P < 0.01), whereas mi R-21 showed positive correlation with ALT values in the samples of autoimmune liver diseases(P < 0.03). The results also revealed a strong correlation between fibrosis stage and LS values(P < 0.01) when etiology of fibrosis was not taken into account.CONCLUSION: Reduced expression of mi R-122 in advanced fibrosis and its correlation with fibrosis stage and LS values seem to be characteristic of hepatic fibrosis of various etiologies. | Tünde Halász Gábor Horváth Gabriella Pár Klára Werling András Kiss Zsuzsa Schaff Gábor Lendvai | 2015 | World Journal of Gastroenterology2015，21，25： | 11 |
| 2 | Therapeutic proteasome inhibition in experimental acute pancreatitis显示文摘AIM: To establish the therapeutic potential of proteasome inhibition, we examined the therapeutic effects of MG132 (Z-Leu-Leu-Leu-aldehyde) in an experimental model of acute pancreatitis.METHODS: Pancreatitis was induced in rats by two hourly intraperitoneal (ip) injections of cholecystokinin octapeptide (CCK; 2 x 100 μg/kg) and the proteasome inhibitor MG132 (10 mg/kg ip) was administered 30 min after the second CCK injection. Animals were sacrificed 4 h after the first injection of CCK.RESULTS: Administering the proteasome inhibitor MG132 (at a dose of 10 mg/kg, ip) 90 min after the onset of pancreatic inflammation induced the expression of cell-protective 72 kDa heat shock protein (HSP72) and decreased DNA-binding of nuclear factor-kB (NF-kB). Furthermore MG132 treatment resulted in milder inflammatory response and cellular damage, as revealed by improved laboratory and histological parameters of pancreatitis and associated oxidative stress.CONCLUSION: Our findings suggest that proteasome inhibition might be beneficial not only for the prevention, but also for the therapy of acute pancreatitis. | Tamás Letoha Liliána Z Fehér László Pecze Csaba Somlai Ilona Varga József Kaszaki Gábor Tóth Csaba Vizler László Tiszlavicz Tamás Takács | 2007 | World Journal of Gastroenterology2007，13，33： | 5 |
| 3 | CYP24A1 inhibition facilitates the anti-tumor effect of vitamin D3 on colorectal cancer cells显示文摘AIM:The effects of vitamin D3 have been investigated on various tumors, including colorectal cancer (CRC). 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1), the enzyme that inactivates the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25-D3), is considered to be the main enzyme determining the biological halflife of 1,25-D3. During colorectal carcinogenesis, the expression and concentration of CYP24A1 increases significantly, suggesting that this phenomenon could be responsible for the proposed efficacy of 1,25-D3 in the treatment of CRC. The aim of this study was to investigate the anti-tumor effects of vitamin D3 on the human CRC cell line Caco-2 after inhibition of the cytochrome P450 component of CYP24A1 activity. METHODS:We examined the expression of CYP24A1 mRNA and the effects of 1,25-D3 on the cell line Caco-2 after inhibition of CYP24A1. Cell viability and proliferation were determined by means of sulforhodamine-B staining and bromodeoxyuridine incorporation, respectively, while cytotoxicity was estimated via the lactate dehydrogenase content of the cell culture supernatant. CYP24A1 expression was measured by realtime reverse transcription polymerase chain reaction. A number of tetralone compounds were synthesized to investigate their CP24A1 inhibitory activity. RESULTS:In response to 1,25-D3, CYP24A1 mRNA expression was enhanced significantly, in a time- and dose-dependent manner. Caco-2 cell viability and proliferation were not influenced by the administration of 1,25-D3 alone, but were markedly reduced by coadministration of 1,25-D3 and KD-35, a CYP24A1-inhibiting tetralone. Our data suggest that the mechanism of action of co-administered KD-35 and 1,25-D3 does not involve a direct cytotoxic effect, but rather the inhibition of cell proliferation. CONCLUSION:These findings demonstrate that the selective inhibition of CYP24A1 by compounds such as KD-35 may be a new approach for enhancement of the anti-tumor effect of 1,25-D3 on CRC. | János P Kósa Péter Horváth János Wlfling Dóra Kovács Bernadett Balla Péter Mátyus Evelin Horváth Gábor Speer István Takács Zsolt Nagy Henrik Horváth Péter Lakatos | 2013 | World Journal of Gastroenterology2013，19，17： | 5 |
| 4 | 经桡动脉行颈动脉狭窄支架成形术的疗效分析显示文摘目的探讨经桡动脉入路行颈动脉狭窄支架成形术的适应证和优势.方法经桡动脉入路在颈内动脉岩部放置阻塞球囊,对38例颈动脉粥样硬化性狭窄患者行支架置入术.其中有症状者29例,无症状者9例.术前均行颈动脉数字减影血管造影(DSA)、脑实质血管DSA、颈动脉多普勒超声及头部MRI等检查.结果术后患者均复查颈动脉DSA和脑实质血管DSA,显示脑部供血均有明显改善,其患侧颈内动脉、皮质下血管显影时间较术前提前0.3~0.8 s,颅内血管血流经过时间缩短0.4~0.7 s.短暂性脑缺血发作(TIA)症状消失.全组无手术死亡病例,无并发症.随访3~15个月,无再狭窄患者.结论当经股动脉入路为相对禁忌时,经桡动脉入路是颈动脉狭窄支架成形术值得选择的一种入路. | 张佳栋 J G Théron | 2005 | 中国脑血管病杂志2005，2，12： | 4 |
| 5 | Microscopic colitis: A retrospective study of clinical presentation in 53 patients显示文摘AIM: To evaluate the relationship between symptoms and microscopic colitis (MC) subtypes: to test whether collagenous colitis (CC) and/or lymphocytic colitis (LC)might be related to both constipation and diarrhea.METHODS: A cohort of patients with independently confirmed typical histopathological changes was investigated. Fifty-three patients with histologically proved MC (46 with CC, 7 with LC) were included. The existence of diarrhea or constipation and the co-existence of autoimmune diseases were also investigated and all data were retrospectively analyzed.RESULTS: Twenty-three (43.39%) of MC patients had chronic constipation (20 in CC, 3 in LC patients). Twentyfour(45.28%) of MC patients had autoimmune disease and the diagnosis of autoimmune disease was always prior to MC. Sjogren's syndrome was associated only with the constipation subgroup.CONCLUSION: The Janus face of MC resembles the subgroups of irritable bowel syndrome. The co-existence of autoimmune diseases and MC is confirmed in both the constipation and diarrhea subgroups. | Zsolt Barta Gabriella Mekkel István Csíp(o|') László Tóth Szabolcs Szakáll Gábor G.Szabó Gyula Bakó Gyula Szegedi Margit Zeher | 2005 | World Journal of Gastroenterology2005，11，9： | 3 |
| 6 | Comparative screening for thyroid disorders in old age in areas of iodine deficiency, long‐term iodine prophylaxis and abundant iodine intake显示文摘 | IstvánSzabolcs JanPodoba JoachimFeldkamp OrsolyaDohán IldikóFarkas MihálynéSajgó Krisztina I.Takáts MiklósGóth LászlóKovács KatalinKressinszky PeterHnilica GézaSzilágyi | 2003 | Clinical Endocrinology2003，，1： | 2 |
| 7 | Synergistic control of sex hormones by 17β-HSD type 7: a novel target for estrogen-dependent breast cancer显示文摘17β-hydroxysteroid dehydrogenase(17β-HSD)type 1 is known as a critical target to block the final step of estrogen production in estrogen-dependent breast cancer.Recent confirmation of the role of dyhydroxytestosterone(DHT)in counteracting estrogeninduced cell growth prompted us to study the reductive 17β-HSD type 7(17β-HSD7),which activates estrone while markedly inactivatingDHT.The role ofDHTin breast cancer cell proliferation isdemonstratedby its independent suppression of cell growthin the presence of a physiological concentration of estradiol(E2).Moreover,an integral analysis of a large number of clinical samples in Oncomine datasets demonstrated the overexpression of 17β-HSD7 in breast carcinoma.Inhibition of 17β-HSD7 in breast cancer cells resulted in a lower level of E2 and a higher level of DHT,successively induced regulation of cyclinD1,p21,Bcl-2,and Bik,consequently arrested cell cycle in the G0/G1 phase,and triggered apoptosis and auto-downregulation feedback of the enzyme.Such inhibition led to significant shrinkage of xenograft tumors with decreased cancer cell density and reduced 17β-HSD7 expression.Decreased plasma E2 and elevated plasma DHT levels were also found.Thus,the dual functional 17β-HSD7 is proposed as a novel target for estrogen-dependent breast cancer by regulating the balance of E2 andDHT.Thisdemonstrates aconceptual advance on the general belief that the major role of this enzyme is in cholesterol metabolism. | Xiaoqiang Wang Catherine Gérard Jean-Francois Thériault Donald Poirier Charles J.Doillon Sheng-Xiang Lin | 2015 | Journal of Molecular Cell Biology2015，7，6： | 2 |
| 8 | Relationship between diabetes mellitus and cataract in Hungary显示文摘AIM:To examine the coexistence of diabetes mellitus(DM)and cataract in Hungary.The effects of DM on the cataract surgical results were also in the focus of analysis.METHODS:Statistical data analysis of the results of the Rapid Assessment of Avoidable Blindness with Diabetic Retinopathy(RAAB+DR)module conducted in Hungary in 2015.This cross-sectional,population-based,national survey included 3523 people aged 50 years and over.Participants of the survey were examined on-site.Visual acuity,main cause for visual impairment(using direct and indirect ophthalmoscopes),in case of best corrected visual acuity(BCVA)≤0.5 and blood glucose level(random test with glucometer)were examined.RESULTS:The prevalence of cataract was 23.4%,and DM was 20.0%.The occurrence of cataract steadily increased with age.Among the examined participants with DM,the prevalence of cataract was significantly(P=0.012)higher(+35%)than that in non-diabetic subjects(29.5%vs 21.8%).Following aging(OR=15.2%,P<0.001),DM proved to be the most independent influencing risk factor(OR=49.9%,P<0.001).The presence of DM was neither an influencing factor for complications of cataract surgery,nor for postoperative visual acuity.CONCLUSION:DM appears to be one of the main risk factors for developing cataract.Other risk factors,such as age,sex and environment also play an influencing role.Diabetes does not seem to affect the occurrence of cataract surgical complications. | Anita Pék Dorottya Szabó Gábor LászlóSándor Gábor Tóth András Papp Zoltán Zsolt Nagy Hans Limburg János Németh | 2020 | International Journal of Ophthalmology(English edition)2020，13，5： | 2 |
| 9 | 显示文摘 | Péter Antal Torok Gabriella Tóth Géza | 1998 | Journal of Chromatography A1998，797，： | 2 |
| 10 | Strength prediction of bolted joints in graphite/epoxy composite laminates显示文摘 | K.I Tserpes G Labeas P Papanikos Th Kermanidis | 2002 | Composites Part B2002，，7： | 2 |
| 11 | Friction and wear of carbon fibre filled polymer composites at room and low temperatures 显示文摘 | Theiler G Habner W Gradt Th | 2004 | Material Wissenschaft and Werkstoff Technik2004，35，1011： | 1 |
| 12 | Grinding of WC - Co hardmetals显示文摘 | HEGEMANJBJ W DE HOSSONJ Th M DE With G | 2001 | Wear2001，，248： | 1 |
| 13 | Stability and Electrophoretic Deposition of Suspensions in Non-Aqueous Media显示文摘 | Koelmans H Overbeek J Th G | 1954 | Discuss Faraday Society1954，18，： | 1 |
| 14 | The African catfish,Clarias gariepinus,a model for the study of reproductive endocrinologyin teleosts显示文摘 | VAN OORDT P G W J GOOS H J Th | 1987 | Aquaculture1987，63，： | 1 |
| 15 | Rosetta: A com- puter program for estimating soil hydraulic parameters with hierar- chical pedotransfer functions 显示文摘 | Schaap M G Leij F J Van Genuchten M Th | 2001 | Journal of Hydrology2001，251，34： | 1 |
| 16 | An aluminium heat switch made from cold-pressed Cu/A1 composite显示文摘 | Wang H L Wagner Th Eska G | 2000 | Physica B2000，28，4288： | 1 |
| 17 | Problem-basexi learning of research skills显示文摘 | Davis TH 3rd Wagner GS Gleim G | 2006 | J Electrocardiol2006，39，1： | 1 |
| 18 | Complete Characterization of a Broadband High - Finesse Cavity using an Optical Frequency Comb显示文摘 | Schliesser g Gohle C Udem Th | | 0，，： | 1 |
| 19 | Would you mind if I get more? An experimental study of the envy game显示文摘 | Sandro Casal Werner Güth Mofei Jia Matteo Ploner | 2012 | Journal of Economic Behavior and Organization2012，，3： | 1 |
| 20 | General cone-beam reconstruction algorithm显示文摘 | Wang G Lin TH Cheng PC | 1993 | IEEE Transactions on Medical Imaging1993，12，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员