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| 1 | The characteristics of CO_2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice显示文摘With PEPC, PPDK, NADP-ME and PEPC+ PPDK transgenic and untransformed rice (Orysa sativa L.), the activities of related C4 photosynthesis enzymes, the chlorophyll fluorescence parameters, CO2 exchange and other physiological indexes were compared, in which the physiological characteristics of PEPC transgenic rice were mainly studied. The results were as follows: (i) The activities of PEPC in PEPC transgenic rice were 20-fold higher than those in untransformed rice; the light-saturation photosynthetic rates and the carboxylation efficiency of PEPC transgenic rice were increased by 55% and 50% more than those of untransformed rice, respectively, while the CO2 compensation point decreased by 27%. (ii) The PS II photochemical efficiency (Fv/Fm) and photochemical quenching (qp) of transgenic PEPC rice decreased less in comparison with those of untransformed rice after the treatment with high light intensity (3 h) or methyl viologen (MV), a photooxidative reagent, which demonstrated that the tolerance of PEPC | JIAO Demao, LI Xia, HUANG Xueqing, CHI Wei, KUANG Tingyun & Ku SB Maurice1. Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nangjing 210014, China 2. Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China 3. Botany Department, Washington State University, Pullman, WA 99164-4236, USA | 2001 | Chinese Science Bulletin2001,46,13: | 29 |
| 2 | Inhibitory effect of Salicornia europaea on the marine alga Skeletonema costatum显示文摘Exploiting the negative biochemical interference between plants and algal species has been suggested as a method to control harmful algal blooms.In this work,we investigated the inhibitory effect of the salt marsh halophyte Salicornia europaea against the marine alga Skeletonema costatum.S.europaea suppressed the growth of S.costatum in a nutrient-sufficient co-culture system,indicating that the inhibition of algal growth was because of the phytotoxic effect of S.europaea,rather than nutrient competition.We tested aqueous and organic extracts from S.europaea roots against S.costatum.The organic extracts inhibited growth and affected the cell size and chlorophyll a content of S.costatum in a dose-dependent manner.Among the three tested organic extracts,the methanol extract had the greatest effects on S.costatum,followed by butanol extract,and then the chloroform extract.Two flavonoids,rutin and quercetin-3-D-glucoside,were identified in the methanol extract by high performance liquid chromatography.The concentration of rutin was much higher than that of quercetin-3-D-glucoside.In an algal bioassay,rutin inhibited the growth of S.costatum and the inhibitory effect increased with increasing rutin concentration and with decreasing initial algal density.Therefore,we concluded that S.europaea negatively affects the growth of S.costatum,and that rutin,a metabolite of S.europaea,may play a role in this inhibitory effect. | JIANG Dan HUANG LingFeng LIN YongQing NIE LingLing LV SuLian KUANG TingYun LI YinXin | 2012 | Science China(Life Sciences)2012,55,6: | 8 |
| 3 | Cavitation Optimization for a Centrifugal Pump Impeller by Using Orthogonal Design of Experiment显示文摘Cavitation is one of the most important performance of centrifugal pumps. However, the current optimization works of centrifugal pump are mostly focusing on hydraulic efficiency only, which may result in poor cavitation performance. Therefore, it is necessary to find an appropriate solution to improve cavitation performance with acceptable efficiency. In this paper, to improve the cavitation performance of a centrifugal pump with a vaned diffuser, the influence of impeller geometric parameters on the cavitation of the pump is investigated using the orthogonal design of experiment(DOE) based on computational fluid dynamics. The impeller inlet diameter D_1, inlet incidence angle Δβ, and blade wrap angle φ are selected as the main impeller geometric parameters and the orthogonal experiment of L_9(3*3) is performed. Three-dimensional steady simulations for cavitation are conducted by using constant gas mass fraction model with second-order upwind, and the predicated cavitation performance is validated by laboratory experiment. The optimization results are obtained by the range analysis method to improve cavitation performance without obvious decreasing the efficiency of the centrifugal pump. The internal flow of the pump is analyzed in order to identify the flow behavior that can affect cavitation performance. The results show that D_1 has the greatest influence on the pump cavitation and the final optimized impeller provides better flow distribution at blade leading edge. The final optimized impeller accomplishes better cavitation and hydraulic performance and the NPSHR decreases by 0.63 m compared with the original one. The presented work supplies a feasible route in engineering practice to optimize a centrifugal pump impeller for better cavitation performance. | PEI Ji YIN Tingyun YUAN Shouqi WANG Wenjie WANG Jiabin | 2017 | Chinese Journal of Mechanical Engineering2017,30,1: | 6 |
| 4 | Biomimetic strategies for tendon/ligament-to-bone interface regeneration显示文摘Tendon/ligament-to-bone healing poses a formidable clinical challenge due to the complex structure,composition,cell population and mechanics of the interface.With rapid advances in tissue engineering,a variety of strategies including advanced biomaterials,bioactive growth factors and multiple stem cell lineages have been developed to facilitate the healing of this tissue interface.Given the important role of structure-function relationship,the review begins with a brief description of enthesis structure and composition.Next,the biomimetic biomaterials including decellularized extracellular matrix scaffolds and synthetic-/natural-origin scaffolds are critically examined.Then,the key roles of the combination,concentration and location of various growth factors in biomimetic application are emphasized.After that,the various stem cell sources and culture systems are described.At last,we discuss unmet needs and existing challenges in the ideal strategies for tendon/ligament-to-bone regeneration and highlight emerging strategies in the field. | Tingyun Lei Tao Zhang Wei Ju Xiao Chen Boon Chin Heng Weiliang Shen Zi Yin | 2021 | Bioactive Materials2021,6,8: | 6 |
| 5 | Over 255 mW single-frequency fiber laser with high slope efficiency and power stability based on an ultrashort Yb-doped crystal-derived silica fiber显示文摘A single-frequency distributed Bragg reflector (DBR) fiber laser at 1030 nm has been demonstrated using a 0.7 cm long homemade Yb:YAG crystal-derived silica fiber (YCDSF).The absorption and emission cross sections of the YCDSF are 4.93×10^(-20)cm^(2) at 980 nm and 1.1×10^(-20)cm^(2)at 1030 nm.Using this gain fiber,an over 255 m W continuous-wave lasing in the constructed laser has been obtained at single transverse and longitudinal mode operation.The slope efficiency and the pump threshold of the fiber laser were up to ~35%and as low as 25 m W,respectively.The fiber laser also demonstrated an optical signal-to-noise ratio of 79 dB and a beam quality factor of 1.016 in two orthogonal directions.Its power fluctuation at 210.5 m W was less than 0.85%of the average power within 13 h.Moreover,the relative intensity noise and linewidth of the laser are also investigated at the different pump powers.The results indicate that the single-frequency DBR fiber laser has potential applications in high-quality seed sources and high-precision optical sensing. | YING WAN JIANXIANG WEN CHEN JIANG FENGZAI TANG JING WEN SUJUAN HUANG FUFEI PANG TINGYUN WANG | 2021 | Photonics Research2021,9,5: | 6 |
| 6 | New structure model of oxygen-evolving center and mechanism for oxygen evolution in photosynthesis显示文摘So far, many important questions and problems concerning the structure and mechanism of photosynthetic oxygen evolution are still unsolved. On the basis of recent achievements in this field, a new structure model is proposed whereby two H2O molecules bind asymmetrically to two manganese ions (Mn1Ⅱ and Mn4Ⅲ) at the open end of 'C' shaped cluster and keep rather large distance. Two histidine residues coordinate to the other two manganese ions in higher oxi- | Chunxi Zhang Jie Pan Liangbi Li Tingyun Kuang | 1999 | Chinese Science Bulletin1999,44,24: | 6 |
| 7 | Wavelength-switchable vortex beams based on a polarization-dependent microknot resonator显示文摘We experimentally demonstrated a method of generating continuously wavelength-switchable optical vortex beams(OVBs) in an all-fiber laser. A polarization-dependent microknot resonator(MKR) functions as comb filter and accounts for the narrow linewidth(0.018 nm) of multiwavelength channels. The wavelength interval corresponds to the free spectral range of the MKR. We exploit a fused SMF–FMF(single mode fiber–few mode fiber) mode coupler to obtain broadband mode conversion and successfully achieve multiwavelength switchable OVBs. As far as we know, this is the first report about identical multiwavelength vortex beams with topological charges of 1. It has been verified that each channel of the vortex beams preserves the same orbital angular momentum(OAM) properties through their clear spiral interferograms. Multiwavelength vortex beams with identical OAM properties are desirable for multiplexing, exchanging, and routing to further improve the capacity of optical fiber transmission. | JINQIU ZHENG Ao YANG TENG WANG XIANGLONG ZENG NING CAO MEI LIU FUFEI PANG TINGYUN WANG | 2018 | Photonics Research2018,6,5: | 5 |
| 8 | Distributed Vibration Sensor With Laser Phase-Noise Immunity by Phase-Extraction φ-OTDR显示文摘We have demonstrated a distributed vibration sensor based on phase-sensitive optical time-domain reflectometer (φ-OTDR) system exhibiting immunity to the laser phase noise. Two laser sources with different linewidth and phase noise levels are used in the φ-OTDR system, respectively. Based on the phase noise power spectrum density of both lasers, the laser phase is almost unchanged during an extremely short period of time, hence, the impact of phase noise can be suppressed effectively through phase difference between the Rayleigh scattered light from two adjacent sections of the fiber which define the gauge length. Based on the phase difference method, the external vibration can be located accurately at 41.01 km by the(φ-OTDR system incorporating these two lasers. Meanwhile, the average signal-to-noise ratio (SNR) of the retrieved vibration signal by using Laser I is found to be -37.7 dB, which is comparable to that of -37.5 dB by using Laser II although the linewidth and the phase noise level of the two lasers are distinct. The obtained results indicate that the phase difference method can enhance the performance of(φ-OTDR system with laser phase-noise immunity for distributed vibration sensing, showing potential application in oil-gas pipeline monitoring, perimeter security, and other fields. | Yuying SHAO Huanhuan LIU Peng PENG Fufei PANG Guoqin YU Zhen CHEN Na CHEN Tingyun WANG | 2019 | Photonic Sensors2019,9,3: | 5 |
| 9 | Theoretical aspects and sensing demonstrations of cone-shaped inwall capillary-based microsphere resonators显示文摘In this paper, a detailed theoretical study on the characteristics of cone-shaped inwall capillary-based microsphere resonators is described and demonstrated for sensing applications. The maximum, minimum, slope, contrast, and width of the Fano resonance are analyzed. As the transmission coefficient of the capillary resonator increases, the absolute value of the slope of Fano resonances increases to reach its maximum, which is useful for sensors with an ultra-high sensitivity. There occurs another phenomenon of electromagnetically induced transparency when the reflectivity at the capillary–environment interface is close to 100%. We also experimentally demonstrated its capability for temperature and refractive index sensing, with a sensitivity of 10.9 pm∕°C and 431 d B∕RIU basedon the Fano resonance and the Lorentzian line shape, respectively. | XIAOBEI ZHANG YONG YANG HUAWEN BAI JIAWEI WANG MING YAN HAI XIAO TINGYUN WANG | 2017 | Photonics Research2017,5,5: | 5 |
| 10 | Embedded whispering-gallery mode microsphere resonator in a tapered hollow annular core fiber显示文摘We propose and demonstrate a tapered hollow annular core fiber (HACF) coupler for excitation of whisperinggallery modes (WGMs) of an embedded microsphere resonator. The coupler is simply fabricated by fusion splicing of a segment of HACF with the single-mode fiber (SMF), and then improved by tapering the splicing joint to reduce the cone-apex angle. Therefore, the coupling efficiency from the SMF to the HACF is enhanced to excite various WGMs via evanescent field coupling. Normal positive, negative symmetrical Lorentzian and asymmetric Fano line shapes can be obtained by varying the resonator size and location. Another interesting phenomenon is observed that a higher Q-factor mode in a lower Q-factor mode has a contrast as high as 58. Temperature sensing with good stability is also demonstrated. This embedded WGM microsphere resonator in the tapered HACF is expected to promote environmental adaptability in practical applications due to its simplicity and | JIAWEI WANG XIAOBEI ZHANG MING YAN LEI YANG FENGYU HOU WEN SUN XIAOTONG ZHANG LIBO YUAN HAI XIAO TINGYUN WANG | 2018 | Photonics Research2018,6,12: | 4 |
| 11 | 33 ku protein associated several polypeptides with nearly the same molecular weight but not the same isoelectric point显示文摘The 33 ku protein, prepared from NaCI-treated PSII particles, has shown an single band by SDS-PAGE. After being dialyzed against the low-osmotic medium at 4癈, it has been found that the 33 ku protein degraded into several small fragments. This result suggests that the preparations of 33 ku protein probably contain some latent proteinases. it has also been found, by the 2-D electrophoresis and IEF, that the preparations of 33 ku protein not dialyzed against the low-osmotic medium contain several polypeptides with nearly the same molecular weight but not the same isoelectric point as the 33 ku protein. | Hui Yu Bin Kang Jie Wei Liangbi Li Tingyun Kuang | 2000 | Chinese Science Bulletin2000,45,1: | 3 |
| 12 | Comparative Analysis of the Endosperm Proteins Separated by 2-D Electrophoresis for Two Cultivars of Hybrid Rice (Oryza sativa L,)显示文摘Liangyoupeijiu 是 two-parental-line,并且 Shanyou63 是 three-parental-line 混血儿米饭(Oryza sativa L.) 。尽管两个属于 indica 亚种,他们关于形态学,生理学和谷物质量有明显的差别。在内乳蛋白质作文的变化被比较电气泳动(2-DE ) 为混合米饭的这二栽培变种印射的 2-D 学习。在飞行团 spectrometry (MALDI-TOF/MS ) 的帮助矩阵的激光解吸附作用 / 电离时间以后分析, 19-kDa 血球素的一位 21-kDa 先锋为两栽培变种作为主要存储蛋白质被识别。peroxiredoxin 和种子成熟蛋白质的某 isoforms 被发现仅仅在 Shanyou63 存在,而 aldose reductase 和淀粉小粒界限淀粉 synthase 仅仅在 Liangyoupeijiu 被检测。这些数据可能为混合米饭的这二栽培变种的进一步比较的研究提供一个基础。 | Pingfang Yang Shihua Shen Tingyun Kuang | 2006 | Journal of Integrative Plant Biology2006,48,9: | 3 |
| 13 | Positional distribution of fatty acids on the glycerol backbone during the biosyn-thesis of glycerolipids in Ectocarpus fasciculatus显示文摘The biosynthesis of glycolipids in E.fascicula-tus was studied by 14C label and chase. The fatty acids in sulphoquinovosyl diacylglycerol (SQDG) were almost 16-car-bon and 18-carbon ones. In addition to the two fatty acids, monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) contained 8.5 mol% and 31.0 mol% of eicosapentaenoic acid (20 : 5), respectively, and this fatty acid was usually distributed in the sn-1 position of the glyc-erol backbone. When plants were incubated with [2-14C] acetate, differences existed in the positional distribution of the labeled fatty acids in sn-1 and sn-2 among the three glycerolipids. In SQDG, 14C-labeled fatty acids were distributed uniformly in the sn-1 and sn-2 positions. In DGDG, 14C-labeled fatty acids were mainly distributed in the sn-2 position. In MGDG, the radioactivity of fatty acids in sn-1 position was far greater than that in sn-2 position after a 30 min pulse label, and the difference in radioactivity between the two positions | XU Yinong WANG Zeneng VAN Xiaojun LIN Wei LI Liangbi KUANG Tingyun | 2002 | Chinese Science Bulletin2002,47,21: | 3 |
| 14 | A breakthrough of artificial photosynthesis显示文摘Photosynthesis in plants,algae and cyanobacteria uses light energy from the Sun to convert CO2 and water into carbohydrates and oxygen,thus sustaining all aerobic life forms on Earth.Photosynthetic water splitting into oxygen,protons and electrons,is catalyzed by the oxygen-evolving center(OEC) | Tingyun Kuang | 2016 | National Science Review2016,3,1: | 3 |
| 15 | Black Phosphorus-Film With Drop-Casting Method for High-Energy Pulse Generation From Q-Switched Er-Doped Fiber Laser显示文摘We have reported high-energy pulse generation from a Q-switched Er-doped fiber laser based on black phosphorus (BP) with a drop-casting method. Poly dimethyl diallyl ammonium (PDDA) is employed to protect BP. A passive Q-switching operation is achieved by using the BP/polyvinyl alcohol (PVA) film as the saturable absorbers (SAs). When the pump power increases from 130.4mW to 378mW, the repetition rate increases from 13.33kHz to 26.6kHz, and the pulse duration decreases from 10.67p,s to 7.11 (is. The maximum pulse energy is 468.03 nJ, to the best of our knowledge, which is the highest pulse energy produced from Q-switched fiber laser based on BP-SA at 1.5 pm. The obtained high-energy pulse demonstrates that BP/PVA film fabricated by such a drop-casting method can provide an ideal SA for high pulse energy generation from fiber lasers, and it has a potential application of remote sensing. | Huanhuan LIU Wei SONG Ye YU Qiao JIANG Fufei PANG Tingyun WANG | 2019 | Photonic Sensors2019,9,3: | 3 |
| 16 | Broadband Acoustic Vibration Sensor Based on Cladding-Mode Resonance of Double-Cladding Fiber显示文摘We have proposed and demonstrated a double-cladding fiber (DCF) with cladding?mode resonance property for broadband acoustic vibration sensing. Since the fundamental mode in the core waveguide is able to be coupled to LP05 mode in the tube waveguide once the phase-matching condition is fulfilled, the transmission spectrum can exhibit a dip with a large extinction ratio. An acoustic vibration could induce the wavelength shift of such transmission spectrum, so that the intensity variation at a wavelength near the dip is coded with the information of the acoustic vibration signal. By demodulating the response of intensity variation, the frequency of the applied acoustic vibration signal can be recovered. Such a DCF-based sensor with an intensity modulation could measure the acoustic vibration with a broadband frequency range from 1 Hz to 400 kHz and exhibits the maximum signal-to-noise ratio (SNR) of ?80.79 dB when the vibration frequency is 20 kHz. The obtained results show that the proposed DCF-based acoustic vibration sensor has a potential application in environmental assessment, structural damage detection, and health monitoring. | Guanghui SUI Huanhuan LIU Fufei PANG Jiajing CHENG Tingyun WANG | 2019 | Photonic Sensors2019,9,3: | 2 |
| 17 | Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution显示文摘We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5(PGR5)capable of producing hydrogen(H2) for 25 days with more than 30-fold yield increase compared to wild type.Thus, hpm91 displays a higher capacity of H2 production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H2 production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species(ROS) scavenging capacity during sulfur deprivation. This anti-ROS response appears to protect the photosynthetic electron transport chain from photooxidative damage and thereby ensures electron supply to the hydrogenase. | Mei Chen Jin Zhang Lei Zhao Jiale Xing Lianwei Peng Tingyun Kuang Jean-David Rochaix Fang Huang | 2016 | Journal of Integrative Plant Biology2016,58,12: | 2 |
| 18 | Fractal analysis for root growth of plant seedlings under doubled CO_2 concentration显示文摘Fractal geometry was applied and box dimension was used as an indicator to analyze the effects of doubled CO\-2 concentration on the root growth of plant seedlings. Results showed that doubled CO\-2 concentration displayed different effects on root branching characteristics of C\-3 and C\-4 plants. There was an obvious increase of root branches in spring wheat while there were no significant effects on roots of sweet sorghum. In different soil layers, root branching of spring wheat was stimulated and this promotion was most significant in the second layer (10-20 cm), which denoted that elevated CO\-2 altered the root branching pattern. That means higher CO 2 concentration influences not only root growth but also its differentiation and development. | WANG Yiqin 1, ZHANG Huijuan 1, YANG Dian’an 2, BAI Kezhi 2* and KUANG Tingyun 2 1. Shandong Teachers’ University, Jinan 250014, China 2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. * Corresponding author | 1998 | Chinese Science Bulletin1998,43,22: | 2 |
| 19 | Changes in Thermostability of Photosystem Ⅱ and Leaf Lipid Composition of Rice Mutant with Deficiency of Light-harvesting Chlorophyll a/b Protein Complexes显示文摘 | Yunlai Tang Mei Chen Yinong Xu Tingyun Kuang | 2007 | Journal of Integrative Plant Biology2007,49,4: | 2 |
| 20 | Monitoring and identifying pendant droplets in microbottle resonators显示文摘Optofluidic resonators are capable of characterizing various fluidic media.Here,we propose an optofluidic microbottle resonator(OFMBR)that is applied to generate pendant droplets,whose maximum mass is related to the liquid surface tension.Mass and type of droplets forming along the OFMBR stem can be monitored in real time by spectrum variation.As a pendant droplet grows,increased droplet gravity introduces a decreased coupling gap and compressive force between the tapered fiber and OFMBR,leading to a resonance wavelength shift.The operation mechanism of the proposed sensors is validated by theoretical simulation and experimental results.From the experimental spectra,a liquid mass sensor with maximum sensitivity of−3.34pm/mg is obtained,and distilled water and alcohol can be identified.This scheme provides a new thread for droplet generation as well as fluidic properties characterization. | Zijie Wang Xiaobei Zhang Qi Zhang Yiqi Chen Yong Yang Yang Yu Yang Wang Yanhua Dong Yi Huang Tingyun Wang | 2022 | Photonics Research2022,10,3: | 2 |