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| 1 | Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plants from Turkey显示文摘Tomato brown rugose fruit virus(ToBRFV)causes severe fruit loss in tomato(Solanum lycopersicum)and pepper(Capsicum annuum)plants.It is an emerging Tobamovirus that is spreading globally.The major challenge is to develop a reliable method for the detection of the virus,and to better characterize the symptoms it causes.The aims of this study,therefore,were to characterize the symptom development on tomato and pepper plants,and to establish a reliable detection method for the virus.Following infection of the tomato and pepper plants with ToBRFV,the leaves turned chlorotic,mosaic or mottled,while the fruit became rugose,necrotic and marbled,and showed discoloration with yellow or brown spots.Transmission electron microscopy(TEM)revealed single rod-like virus particles characteristic of the Tobamoviruses.Classical reverse transcription PCR(RT-PCR)and quantitative PCR(qPCR)with specific primers and probes confirmed that the virus is ToBRFV.We found that the resistance genes from tomato,Tm-2^(2),and pepper,L1,L2,L3 and L4,did not confer resistance to ToBRFV.Here,we present a PCR-based method as a diagnostic test for detecting ToBRFV in infected seeds.This method will help to prevent further spread of the virus in commercial seeds. | Hakan FIDAN Pelin SARIKAYA Kubra YILDIZ Bengi TOPKAYA Gozde ERKIS Ozer CALIS | 2021 | Journal of Integrative Agriculture2021，20，8： | 2 |
| 2 | Increased plasma homocysteine levels in multiple sclerosis显示文摘 | Sahin S Aksungar FB Topkaya AE | 2007 | Multiple Sclerosis2007，13，7： | 1 |
| 3 | Recovery of germanium and other valuable metals from zinc plant residues显示文摘 | KUL M TOPKAYA Y | 2008 | Hydrometallurgy2008，92，3： | 1 |
| 4 | Synthesis of polyaniline-MnFe2O4-CTAB nanocomposite in ionic liquid:Its magnetic properties显示文摘 | Esir S Shafiua S Topkaya R | 2013 | Journal of Inorganic and Organometallic Polymers and Materials2013，23，： | 1 |
| 5 | Composite shear stud strength at early concrete ages显示文摘 | Topkaya C | 2004 | Journal of Structural Engineering2004，130，6： | 1 |
| 6 | An Experimental Study on Steel-en- cased Buckling-restrained Brace Hysteretic Dampers显示文摘 | Eryasar ME Topkaya C | 2010 | EarthqEng Struct Dyn2010，39，5： | 1 |
| 7 | Natural periods of steel plate shear wall systems显示文摘 | Topkaya C Kurban C O | 2009 | Journal of Construction- al Steel Research2009，65，3： | 1 |
| 8 | Ischaemia-modi- lied albumin elevation after percutaneous coronary inter- vention reflects albumin concentration rather than is- chaemia显示文摘 | Demir H Topkaya BC Erbay AR | 2009 | Ann Clin Biochem2009，46，4： | 1 |
| 9 | Natural periods of steel plate shear wall systems 显示文摘 | Topkaya C Kurban C O | 2009 | Journal of Constructional Steel Research2009，65，3： | 1 |
| 10 | Desulphurization characteristics of ladle furnace slags of a low sulphur steel显示文摘 | KESKIN K GEVECI A TOPKAYA Y A | 2007 | Canadian Metallurgical Quarterly2007，46，1： | 1 |
| 11 | Composite shear stud strength at early concrete ages 显示文摘 | Topkaya C Yura J A Williamson E B | 2004 | Journal of Structural Engineering2004，130，6： | 1 |
| 12 | Composite shear stud strength at early concrete ages显示文摘 | Topkaya C | 2004 | Journal of Structural Engineering2004，130，6： | 1 |
| 13 | Isehaemia- modified albumin elevation after percutaneous coro- nary intervention reflects albumin concentration rath- er than ischaemia显示文摘 | Demir H Topkaya BC Erbay AR | 2009 | Ann Clin Biochem2009，46，4： | 1 |
| 14 | High pressure acid leaching of a refractory lateritic nickel ore 显示文摘 | KAYA S TOPKAYA Y A | 2011 | Miner Eng2011，24，11： | 1 |
| 15 | Lateral stiffness of steel plate shear wall systems 显示文摘 | Topkaya C Atasoy M | 2009 | Thin-Walled Structures2009，47，8： | 1 |
| 16 | Lateral stiffness of steel plate shear wall systems 显示文摘 | TOPKAYA C ATASOY M | 2009 | Thin-walled Structures2009，47，89： | 1 |
| 17 | Zinc and lead extraction from Cinkur leach residues by using hydrometallurgieal method显示文摘 | Rusen A Sunkar A S Topkaya Y A | 2008 | Hydro- metallurgy2008，93，： | 1 |
| 18 | Dechlorination of a zinc dross显示文摘 | Guresin N Topkaya Y A | 1998 | Hydrornetallurgy1998，49，12： | 1 |
| 19 | Sulfuric acid leaching of Turkish chromite concentrate 显示文摘 | Geveci A Topkaya Y Ayhan E | 2002 | Minerals Engi- neering2002，15，： | 1 |
| 20 | Natural periods of steel plate shear wall systems显示文摘 | Topkaya C Kurban C O | 2009 | Journal of Constructional Steel Research2009，65，3： | 1 |
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