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| 1 | Cancer and age related colonic crypt deficiencies in cytochrome c oxidase Ⅰ显示文摘AIM: To investigate whether defi ciency of expressionof cytochrome c oxidase I (CcOI) in colonic crypts is associated with colon cancer.METHODS: The pattern and level of expression of CcOI in non-neoplastic colonic crypts,and in dysplastic tissues,was assessed using standard immunohis-tochemical methods.Biopsies were obtained from individuals undergoing colonoscopies for screening purposes or for a medically indicated reason.Tissue samples were also obtained from surgical colonic resections.Samples from resections were taken from colonic mucosa 1 and 10 cm from tumors and from the tumors themselves.Samples were evaluated for frequency of crypts with reduced or absent expression of CcOI.In most crypts the loss was apparent throughout the entire crypt,while in a small minority the loss was segmental.The strong immunoreactivity using this monoclonal antibody makes the scoring unambiguous.The percent of crypts with reduced or absent expression of CcOI or (infrequent) segmented loss of expression was then calculated.Data analyses were performed using SPSS statistical package 17.0.RESULTS: The average frequency of CcOI deficient crypts (CcOI-DC) is low in individuals between 20 and 39 years of age,with 0.48% ± 0.40% CcOI-DC for women and 1.80% ± 0.35% for men.CcOI-DC increases after age 40 years,so that between the ages of 40 and 44 years the average frequency of CcOI- DC goes up to 5.89% ± 0.84% in women and 2.15% ± 1.27% in men.By 80-84 years of age,the average frequency of CcOI-DC goes up in women to 15.77% ± 0.97% and in men to 22.6% ± 0.65%.The increases in CcOI-DC from ages 40-44 years compared to 80-84 years in women and men are significantly different with P < 0.01.For women over age 60 years,deficiency of CcOI expression is greater in those women who have had a cancer in their colon.The frequency of CcOI-DC,measured in men,increased in tissues adjacent to colon cancer,being 4.03% ± 0.27% in individuals free of neoplasia in the age range 55-64 yearsand 14.13% ± 0.35% in resected histologically normal tissue of men with cancer in the same age range,P < 0.001.Similar signifi cant differences were noted in older age ranges.The frequency of CcOI-DC crypts in the cecum and sigmoid colon of an individual are signifi cantly correlated,with an R2 = 0.414 for women and R2 = 0.528 for men,P < 0.001.This suggests that the factors determining the level of CcOI deficiency act throughout the colon.Most defective crypts are in clusters of two or more,a likely consequence of crypt fission.In the non-neoplastic margins of cancers,crypts are frequently defi cient for CcOI,and such crypts may appear in large clusters,some containing more than 100 defi cient crypts.CcOI defi ciency is also apparent in colon cancers and sometimes involves a large section of the tumor.Overall,CcOI deficient cells can be visualized in segments of crypts,in whole crypts that increase in frequency with age,in crypts undergoing f ission,in clusters of crypts where the clusters increase in size with age,in increased frequency near tumors,in large clusters in the intimate margins of tumors,and in the tumors themselves.There is no clear dividing line between early stages that can be considered aspects of aging and later stages that can be considered aspects of the progression to cancer.This ambiguity may re ect a rather general situation leading to adult cancer where the early stages of cellular change appear to be relatively innocuous features of the aging process but over decades may evolve into malignancy.CONCLUSION: CcOI defi cient crypts increase in frequency with age,and clusters of defi cient crypts are associated with,and may give rise to,colon cancer. | Carol Bernstein Alexander Facista Huy Nguyen Beryl Zaitlin Nadia Hassounah Cristy Loustaunau Claire Margaret Payne Bhaskar Banerjee Steve Goldschmid V Liana Tsikitis Robert Krouse Harris Bernstein | 2010 | World Journal of Gastrointestinal Oncology2010，2，12： | 5 |
| 2 | Impact of the suction line accumulator on the frost/defrost performance of an air-source heat pump with a scroll compressor显示文摘 | Nutter D W O'Neal D L Payne W V | 1996 | ASHRAE Transaction1996，102，1： | 4 |
| 3 | Constructed wetlands for livestock wastewater management显示文摘 | Knight R L Payne Jr V W E Borer R E | 2000 | Ecological Engineering2000，15，： | 1 |
| 4 | A comparison of the mutagenicity of mainstream cigarette smoke condensates from a representative sample of the US cigarette market with a Kentucky reference cigarette (K1R4F) 显示文摘 | Steele R H Payne V M | 1995 | Mutation Research1995，342，： | 1 |
| 5 | Mast cell tryptase : a review of its physiology and clinical significance 显示文摘 | Payne V Kam P C | 2004 | Anaesthesia2004，59，7： | 1 |
| 6 | C/EBP transcription factors regulate SREBPlc gene expression during adipogenesis显示文摘 | Payne V A Au W S Lowe C E | 2010 | Biochem J2010，425，1： | 1 |
| 7 | Impact of breathing 100% oxygen on radiation-induced cognitive impairment显示文摘 | Wheeler KT Payne V DAgostino RB Jr | 2014 | Radiat Res2014，182，5： | 1 |
| 8 | Optimized and transferable nonlocal separable ab initio pseudopotentials显示文摘 | LIN J S QTEISH A PAYNE M C HEINE V | 1993 | Physical Review B1993，47，8： | 1 |
| 9 | Constructed wetlands for livestock wastewater management显示文摘 | Knight R L Payne Jr V W E Borer R E | 2000 | Ecological Engineering2000，15，12： | 1 |
| 10 | Impact of the suction line accumulator on the frost/defrost performance of an air-source heat pump with a scroll compressor显示文摘 | Nutter D W O'Neal D L Payne W V | 1993 | ASHRAE Transaction1993，99，1： | 1 |
| 11 | Impact of the suction line accumulator on the frost/defrost performance of an air source heat pump with a scroll compressor显示文摘 | Nutter D W O'Neal D L Payne V | 1996 | American Society of Heating Refrigerating and Airconditioning Engineers Transctions1996，102，1： | 1 |
| 12 | Electronic structure, properties and phase stability of inorganic crystals: A pseudopotential plane-wave study显示文摘 | MILMAN V WINKLER B WHITE J A PICKARD C J PAYNE M C AKHMATSKAYA E V NOBES R H | 2000 | International Journal of Quantum Chemistry2000，77，5： | 1 |
| 13 | Global satellite measurements of HDO and implications for understanding the transport of water vapour into the stratosphere显示文摘 | PAYNE V H NOONE D DUDHIA A | 2007 | Quarterly Journal of the Royal Meteorological Society2007，133，627： | 1 |
| 14 | Ground-state properties of CoSi2 determined by a total-energy pseudopotential method显示文摘 | MILMAN V LEE M H PAYNE M C | 1994 | Physical Review B1994，49，16： | 1 |
| 15 | Mono-lithic GaAs p-i-n switch circuits for high power millimiter-wave application 显示文摘 | Bellantoni J V Battle D C Payne D | 1989 | IEEE Transactions on Microwave Theory and Techniques1989，37，12： | 1 |
| 16 | Absolute rate constant for the reaction of atomic hydrogen with phosphine over the temperature range 209 to 495 K 显示文摘 | Lee J H Michael J V Payne W A | 1976 | J Chem Phys ( S0021-9606)1976，65，8： | 1 |
| 17 | Alternative fuel reburning显示文摘 | ZAMANSKY V M HO L PAYNE R | 1999 | Fuel1999，78，3： | 1 |
| 18 | Constructed wetland for livestock waster management 显示文摘 | Knight R L Payne V W Borer R E | 2000 | Ecological Engineering2000，15，： | 1 |
| 19 | Proliposomes:A novel solution to an old problem显示文摘 | Payne N I Timmins P Ambrose C V | 1986 | J Pharm Sci1986，75，4： | 1 |
| 20 | Ground-state properties of CoSi2 determined by a total-energy pseudopotential method 显示文摘 | MILMAN V LEE M H PAYNE M C | 1994 | Physcal Review B1994，49，16： | 1 |
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