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| 1 | The complete reference genome for grapevine (Vitis vinifera L.) genetics and breeding显示文摘Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,limiting the accessibility of the repetitive sequences,the centromeric and telomeric regions,and the study of inheritance of important agronomic traits in these regions.Here,we assembled a telomere-to-telomere(T2T)gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads.The T2T reference genome(PN_T2T)is 69 Mb longer with 9018 more genes identified than the 12X.v0 version.We annotated 67%repetitive sequences,19 centromeres and 36 telomeres,and incorporated gene annotations of previous versions into the PN_T2T assembly.We detected a total of 377 gene clusters,which showed associations with complex traits,such as aroma and disease resistance.Even though PN40024 derives from nine generations of selfing,we still found nine genomic hotspots of heterozygous sites associated with biological processes,such as the oxidation–reduction process and protein phosphorylation.The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs. | Xiaoya Shi Shuo Cao Xu Wang Siyang Huang Yue Wang Zhongjie Liu Wenwen Liu Xiangpeng Leng Yanling Peng Nan Wang Yiwen Wang Zhiyao Ma Xiaodong Xu Fan Zhang Hui Xue Haixia Zhong Yi Wang Kekun Zhang Amandine Velt Komlan Avia Daniela Holtgräwe Jérôme Grimplet JoséTomás Matus Doreen Ware Xinyu Wu Haibo Wang Chonghuai Liu Yuling Fang Camille Rustenholz Zongming Cheng Hua Xiao Yongfeng Zhou | 2023 | Horticulture Research2023，10，5： | 3 |
| 2 | The MOS 36-Item Short-Form Health Survey (SF-36) : Ⅲ : Tests of data quality, scaling assumptions and reliability across diverse patient groups显示文摘 | Mcklorney C A Ware J E Lu J F R | 1994 | Med Care1994，32，1： | 1 |
| 3 | Membrane thinning effect of the betasheet antimicrobial protegrin显示文摘 | Heller W T Waring A J Lehrer R I | 2000 | Biochemistry2000，39，： | 1 |
| 4 | Predicting fine root production and turnover by monitoring root starch and soil temperature显示文摘 | Marashall J D Waring R H | 1985 | Can J For Res1985，15，： | 1 |
| 5 | Comparison of methods of estimating leaf-area index in old-growth Douglas-fir显示文摘 | Marshall J D Waring R H | 1986 | Ecology1986，67，： | 1 |
| 6 | Rock- en, Propagation Delays Induced in GPS Signals by Dry Air, Water Vapor, Hydrometeors and Other Particulates显示文摘 | Solheim F Vivekanandan J Ware R | 1997 | Journal of Geophysical Research1997，104，8： | 1 |
| 7 | A multichannel radiometric profiler of temperature, humidity, and cloud liquid显示文摘 | Ware R Carpenter R Guldner J | 2003 | Radio Science2003，38，4： | 1 |
| 8 | The MOS short-form general health survey: reliability and validity in a patient population显示文摘 | Stewart A L Hays R D Ware J E Jr | 1988 | Med Care1988，26，7： | 1 |
| 9 | Survey of microorganisms for the production of extracellular phytase 显示文摘 | Shieh T R J H Ware | 1968 | Apple Microbiol1968，16，： | 1 |
| 10 | Glycosyl-phosphatidylinositol anchor synthesis in paroxysmal nocturnal hemoglobinuria:partial or complete defect in an early step显示文摘 | Norris J Hall S ware R E | | 0，，03： | 1 |
| 11 | Platelet function beyondhemostasis and thrombosis 显示文摘 | Ware J Corken A Khetpai R | 2013 | Curr Opin2013，20，5： | 1 |
| 12 | Precision of geomagnetic field measurements in a tectonically active region显示文摘 | Johnston M J S Mueller R J Ware R H | 1984 | J Geomag Geoelectr1984，36，： | 1 |
| 13 | Impact of single-residue mutations on the structure and function of ovispirin/novispirin antimicrobial peptides显示文摘 | Sawai M V Waring A J Kearney W R | 2002 | Protein Eng2002，15，3： | 1 |
| 14 | Survey of microorganism for the production of ex- tracellular phytase显示文摘 | Shieh T R Ware J H | 1968 | Appl Microbiol1968，16，： | 1 |
| 15 | Comparison of methods of estimating leaf-area index in old-growth Douglas-fir显示文摘 | MARSHALL J D WARING R H | 1986 | Ecology1986，67，： | 1 |
| 16 | Iron disilicide as a thermoelectric generator material 显示文摘 | Ware R M McNeill D J | 1964 | Electrical Engineers Proc Institution1964，111，1： | 1 |
| 17 | Predicting fine root production and turnover by monitoring root starch and soil temperature显示文摘 | MARSHALL J D WARING R H | 1985 | Canadian Journal of Forest Research1985，15，5： | 1 |
| 18 | Nucleotide sequence of a cDNA for a member of the human 90-kDa heat-shock protein family显示文摘 | Rebbe N F Ware J Bertina R M | 1987 | Gene1987，53，23： | 1 |
| 19 | Impact of sin- gle- residue mutations on the structural and function of ovispirin/ novispirin antimierobial peptides 显示文摘 | SAWAI M V WARING A J KEARNEY W R | 2002 | Protein Eng2002，15，3： | 1 |
| 20 | Predicting fine root production and turnover by monitoring root starch and soil temperature显示文摘 | Marshall J D Waring R H | 1985 | Canadian Journal of Forest Research1985，15，5： | 1 |
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