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| 1 | Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines显示文摘AIM To observe the effect of acupuncture and moxibustion on the expression of IL 1β and IL 6 mRNA in ulcerative colitis rats. METHODS The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000*!r/*!min . The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (no Freund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n =8), electro acupuncture group (EA, n =8), herbs partition moxibustion group (HPM 8), normal control group (NC, n =8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc ) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT PCR technique was used to study the expression of IL 1β and IL 6 mRNA. RESULTS IL 1β and IL 6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats. IL 1β and IL 6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL 1β and IL 6 mRNAs in the EA and HPM rats. The expressions of IL 1β and IL 6 mRNAs were nearly the same in the spleen and colon of all groups. CONCLUSION Acupuncture and moxibustion greatly inhibited the expression of IL 1β and IL 6 mRNA in the experimental ulcerative colitis rats. | Wu HG Zhou LB Pan YY Huang C Chen HP Shi Z Hua XG | 1999 | World Journal of Gastroenterology1999，5，6： | 45 |
| 2 | Cloning and identification of an angiostatic molecule IP-10/crg-2显示文摘AIM To obtain human and murine cDNAsencoding IFN--y inducible protein 10 (lP--10) andcytokine responsive gene--2 (Crg-2).METHODS The encoding genes of lP-10 and Crg2 were amplified by RT--PCR from Cultured humanfibroblast cells and Balb/ c mouse liver treatedby IFN-y and TNF-a, respectively, and clonedinto plasmids of PUC19 and PGEM3Zf(+ ).RESULTS The nucleotide sequences ot theamplified DNA were confirmed by endonucleasesdigestion and sequencing.CONCLUSION Recombinant lP-10/ crg--2 geneclones with 306 hp and 314 hp inserts wereestablished for further research on biologicalactivities and ligands of hiP-10/mCrg--2. | Liu ZG Yang JH An HZ Wang HY He FT Han ZY Han Y Wu HP Xiao B Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 2 |
| 3 | Serial cytokine levels in pa- tients with severe sepsis显示文摘 | Wu HP Chen CK Chung K | 2009 | Inflamm Res2009，5，： | 1 |
| 4 | Therapeutic efficacy of bipolar plasmakinetic technique compared with transurethral resection on benign prostate hyperplasia 显示文摘 | Wu WJ Wang XH Wang HP | 2005 | Zhonghua Yi Xue Za Zhi2005，85，47： | 1 |
| 5 | Computer access assess- ment for persons with physical disabilities: a guide to assistive technology interventions 显示文摘 | Wu TF Meng LF Wang HP | 2002 | Computers Helping People with Special Needs Lecture Notes in Computer Science2002，2398，： | 1 |
| 6 | Intratympanic steroid injections as a salvage treatment for sudden sensorineural hearing loss: a randomized, double-blind, placebo-controlled study 显示文摘 | Wu HP Chou YF Yu SH | 2011 | Otol Neurotol2011，32，5： | 1 |
| 7 | Expression of Lgr5 in human colorectal carcinogenesis and its potential correlation with 15-catenin 显示文摘 | Fan XS Wu HY Yu HP | 2010 | Int J Colorectal Dis2010，25，5： | 1 |
| 8 | Taurine prevents high-glucose-induced human vascular endothelial cell apoptosis显示文摘 | Wu QD Wang JH Fennessy F Redmond HP Bouchier-Hayes D | 1999 | Am J Physiol1999，277，61： | 1 |
| 9 | Skin denervation in type 2 dia- betes: correlations with diabetic duration and functional impair- ments 显示文摘 | Shun CT Chang YC Wu HP | 2004 | Brain2004，127，7： | 1 |
| 10 | Suppression of experimental autoimmune encephalomyelitis by selective blockade of encephalitogenic T-cell infiltration of the central nervous system显示文摘 | Yan SS Wu ZY Zhang HP | | 0，，3： | 1 |
| 11 | Mice expressing a humanized form of VEGF-A may provide insights into safety and efficacy of anti-VEGF antibodies显示文摘 | Gerber HP Wu X Yu L | 2007 | Proc Natl Acad Sci U S A2007，104，9： | 1 |
| 12 | 4- (Methylnitrosamino) - 1 - (3-pyridyl) - 1 -b utanone from cigarette smoke stimulates colon cancer growth via beta- adrenoeeptors 显示文摘 | WU WK WONG HP LUO SW | 2005 | Cancer Res2005，65，12： | 1 |
| 13 | Relationship between the depression status of patients with resectable non-small cell lung cancer and their family members in China显示文摘 | Wu XN Su D Li HP | 2013 | Eur J Oncol Nurs2013，17，5： | 1 |
| 14 | A hybrid model combining hydrodynamic and biological effects for production of bacterial cellulose with a pilot scale airlift reactor 显示文摘 | ZUO KW CHENG HP WU SC | 2006 | Biochem Eng J2006，29，： | 1 |
| 15 | Effects of diabetes on female voiding behavior 显示文摘 | Lee WC Wu HP Tai TY | 2004 | J Urol2004，172，3： | 1 |
| 16 | Skin denervation in type 2 diabetes:correlations with diabetic duration and functional impairments显示文摘 | Shun CT Chang YC Wu HP | 2004 | Brain2004，127，7： | 1 |
| 17 | Predictors of survival and neuralogic outcomes in children with traumatic out-of-hospital cardiac arrest during the early postresuseitative period显示文摘 | Lin YR Wu HP Chen WL | 2013 | J Trauma Acute Care Surg2013，75，3： | 1 |
| 18 | Antimicrobial susceptibility of Neisseria gonorrhoeae strains isolated in Guangzhou,China, 1996- 2001 显示文摘 | Zheng HP Cao WL Wu XZ | 2003 | Sex Transm Infect2003，79，： | 1 |
| 19 | Comparison of respiratory parameters and plasma cytokine levels between treatment with Salmeterol/fluticasone and ipratropium/terbutaline/budesonide in mechanically ventilated COPD patients显示文摘 | Wu HP Liu YC Lin SC | 2012 | Chang Gung Med J2012，35，5： | 1 |
| 20 | Associations ofT helper 1, 2, 17 and regulatory T lymphocytes with mortality in severe sepsis 显示文摘 | Wu HP Chung K Lin CY | 2013 | Inflamm Res2013，62，8： | 1 |
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