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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 3 | THE DIRECT KINEMATIC SOLUTION OF THE PLANAR STEWART PLATFORM WITH COPLANAR GROUND POINTS显示文摘A procedure of computing the position of the planar Stewart platfrom with coplanar ground points is presented avoiding the computation of Groebner basis by standard algorithm. The polynomial system resulted is triangularized. The number of arithmetic operations needed can be predisely | Wu, WD Huang, YZ | 1996 | Journal of Computational Mathematics1996，14，3： | 7 |
| 4 | Purging of murine erythroblastic leukemia by ZnPcS2P2-based-photodynamic therapy显示文摘 | Huang HF Chen YZ Wu Y Chen P | 2006 | 中国生物学文摘2006，20，5： | 5 |
| 5 | Effet of Maotai liguor in inducing metallothioneins and on hepatic stellate cells显示文摘 | Cheng ML Wu J Wang HQ Xue LM Tan YZ Liu P Li CX Huang NH Yao YM Ren LZ Ye L Li L Jia ML | 2001 | Shijie Huaren Xiaohua Zazhi2001，9，12： | 2 |
| 6 | Implementing controlled-NOT gate based on free spin qubits with semiconductor quantum-dot array显示文摘 | Wu YZ Zhang WM | 2005 | Europhysics Letters2005，71，4： | 1 |
| 7 | A novel correction factor based onextended volume to complement the conformity index 显示文摘 | Jin F Wang Y Wu YZ | 2012 | Br J Radiol2012，85，1016： | 1 |
| 8 | The distrivutive changes of hypotonic anoxic pulmonary artery collagen显示文摘 | Xu ZL Wu KJ Yan YZ | 1995 | Zhongguo Yixue Kexueyuan Xuebao1995，17，3： | 1 |
| 9 | PI3K/Akt/mTOR pathway inhibitors in can- cer: a perspective on clinical progress 显示文摘 | Wu P Hu YZ | 2010 | Curr Med Chem2010，17，35： | 1 |
| 10 | Free radicals, antioxidants, and nutrition 显示文摘 | Fang YZ Yang S Wu G | 2002 | Nutrition2002，18，10： | 1 |
| 11 | Frequencies of epitope- specific cytotoxic T lymphocytes in active chronic viral hepatitis B infection by using MHC class I peptide tetramers 显示文摘 | Wu YZ Zhang J Chen SY | 2004 | Immunol Lett2004，92，3： | 1 |
| 12 | Proteomic analysis of the tumorigenic human prostate cell line M12 after microcell-mediated transfer of chromosome 19 demonstrates reduction of vimentin 显示文摘 | Liu XH Wu YZ Zehner ZE | 2003 | Electrophoresis2003，24，： | 1 |
| 13 | Relationship between endogenous concentrations and serum cholesteryl ester transfer protein concentrations in Chinese women显示文摘 | Zhang CN Zhuang YY Qiang HJ Liu XC Xu RJ Wu YZ | 2001 | Clin Chim Acta2001，314，： | 1 |
| 14 | Dosimetric evaluation of volumetric-modulated arc therapy (RapidAre) for primary leiomyosarcoma in the spine 显示文摘 | Wu YZ Jin F Wang Y | 2014 | Teehnol Cancer Res Treat2014，13，5： | 1 |
| 15 | Free Radicals,Antioxidants and Nutrition显示文摘 | Fang YZ Yang S Wu GY | 2002 | Nutrition2002，18，10： | 1 |
| 16 | Thyroid hormone receptor variant α 2: Role of the ninth heptad in DNA binding,heterodimerization with retinoid X receptors,and dominant negative activity显示文摘 | Yang YZ Burgos TM Wu Y | 1996 | J Biol Chem1996，271，28： | 1 |
| 17 | Interaction of surfactant protein A with peroxiredoxin 6 regulates phospholipase A2 activity显示文摘 | Wu YZ Manevich Y Baldwin JL | | 0，，11： | 1 |
| 18 | Glutathione metabolism and its implications for health显示文摘 | Wu GY Fang YZ Turner ND | | 0，，： | 1 |
| 19 | The PTEN/PI3K/Akt and Wnt/beta-cateninsignaling pathways are involved in the inhibitory effect of resveratrol on human colon cancer cell proliferation显示文摘 | Liu YZ Wu K Huang J | 2014 | Int J Oncol2014，45，1： | 1 |
| 20 | Free radicals, antioxidants,and nutrition 显示文摘 | Fang YZ Yang S Wu G | 2002 | Nutrition2002，18，10： | 1 |
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