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| 1 | An acorn squash (Cucurbita pepo ssp. ovifera) fruit and seed transcriptome as a resource for the study of fruit traits in Cucurbita显示文摘Acorn squash(Cucurbita pepo)is an iconic fall vegetable in the United States,known for its unique fruit shape and also prized for its culinary properties.Little is known about the metabolism that underlies the development of fruit quality attributes such as color,sweetness,texture and nutritional qualities in acorn squash,or any other winter squash grown worldwide.To provide insight into winter squash fruit and seed development and add to the genomic resources in the Cucurbita genus,RNA sequencing was used to generate an acorn squash fruit and seed transcriptome from the cultivar Sweet REBA at critical points throughout fruit development.141838600 high-quality paired-end Illumina reads were assembled into 55949 unigenes.85%of unigenes with predicted open reading frames had homology with previously identified genes and over 62%could be functionally annotated.Comparison with the watermelon and cucumber genomes provided confirmation that the unigenes are full-length and comprehensive,covering an average of 90%of the coding sequence of their homologs and 72%of the cucumber and watermelon exomes.Key candidate genes associated with carotenoid and carbohydrate metabolism were identified toward a resource for winter squash fruit quality trait dissection.This transcriptome represents a major advance in C.pepo genomics,providing significant new sequence information and revealing the repertoire of genes expressed throughout winter squash fruit and seed development.Future studies on the genetic basis of fruit quality and future breeding efforts will be enhanced by tools and insights developed from this resource. | Lindsay E Wyatt Susan R Strickler Lukas A Mueller Michael Mazourek | 2015 | Horticulture Research2015,2,1: | 11 |
| 2 | Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin显示文摘Both the fruit mesocarp and the seeds of winter squash can be used for consumption,although the focus of breeding efforts varies by cultivar.Cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content,percent dry matter,and percent soluble solids,while these traits are essentially ignored in oilseed pumpkins.To compare fruit development in these two types of squash,we sequenced the fruit transcriptome of two cultivars bred for different purposes:an acorn squash,‘Sweet REBA’,and an oilseed pumpkin,‘Lady Godiva’.Putative metabolic pathways were developed for carotenoid,starch,and sucrose synthesis in winter squash fruit and squash homologs were identified for each of the structural genes in the pathways.Gene expression,especially of known rate-limiting and branch point genes,corresponded with metabolite accumulation both across development and between the two cultivars.Thus,developmental regulation of metabolite genes is an important factor in winter squash fruit quality. | Lindsay E Wyatt Susan R Strickler Lukas A Mueller Michael Mazourek | 2016 | Horticulture Research2016,3,1: | 7 |
| 3 | The safety of tenofovir disoproxil fumarate for the treatment of HIV infection in adults: the first 4 years显示文摘 | Mark R Nelson Christine Katlama Julio S Montaner David A Cooper Brian Gazzard Bonaventura Clotet Adriano Lazzarin Knud Schewe Joep Lange Christina Wyatt Sue Curtis Shan-Shan Chen Stephen Smith Norbert Bischofberger James F Rooney | 2007 | AIDS2007,,10: | 2 |
| 4 | Highly efficient 980 nm operation of an Yb3+-doped silica fibre laser显示文摘 | J R Armitage R Wyatt B J Ainslie | | 0,,: | 2 |
| 5 | Structures of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody显示文摘 | Wyatt R Robinson J | 1998 | Nature1998,393,: | 1 |
| 6 | Effects of a media campaign on back pain beliefs and its potential influence on management of low back pain in general practice显示文摘 | Buchbinder R Jolley D Wyatt M | 2001 | Spine2001,26,23: | 1 |
| 7 | IgA nephropathy: Long - term prognosis for pediatric patients显示文摘 | Wyatt R J Kritehevsky SB Woodford SY | 1995 | J Pediatr1995,127,91: | 1 |
| 8 | Broad diversity of neutralizing antibodies isolated from memory B cells in HIV-infected individuals显示文摘 | Scheid J F Mouquet H Feldhahn N Seaman M S Velinzon K Pietzsch J Ott R G Anthony R M Zebroski H Hurley A Phogat A Chakrabarti B Li Y Connors M Pereyra F Walker B D Wardemann H Ho D Wyatt R T Mascola J R Ravetch J V Nussenzweig M C | 2009 | Nature2009,458,7238: | 1 |
| 9 | Early Career out Cames of Graduate Employees: The Effect of Mentoring and in Graduations显示文摘 | Aryee S Wyatt T Stone R | 1996 | Journal of Management Studies1996,33,1: | 1 |
| 10 | Visualizatlon by immune electron microscopy of 27nmp particle asseciated with acute infec-tions nonbacterial gastroentefifis显示文摘 | Kapkian A Z Wyatt R G etal | 1972 | J virol1972,10,: | 1 |
| 11 | The human immunodeficiency virus (HIV-1) Envelope glycoproteins: fusogens,antigens and immunogens显示文摘 | Sodroski J | 1998 | Science1998,288,: | 1 |
| 12 | Multiple effects of artemin on sympathetic neurone generation, survival and growth 显示文摘 | Andres R Forgie A Wyatt S | 2001 | Devel- opment2001,128,19: | 1 |
| 13 | Immunization of rhesus monkeys with a recombinant of modified vaccina virus Ankara expressing a truncated envelope glycoprotein of dengue type 2 virus induced resistance to dengue type 2 virus challenge显示文摘 | Men R Wyatt L Tokimatsu I | 2000 | Vaccine2000,18,27: | 1 |
| 14 | Visualization by immune electron microscopy of a 27 - nm particle associated with acute infectious nonbacterial gastroenteritls 显示文摘 | Kapikian AZ Wyatt RG Dolin R | 1972 | J Virol1972,10,: | 1 |
| 15 | The effect of chlorinated paraffins on hepatic enzymes and thyroid hormones显示文摘 | Wyatt I Coutss C T Elcombe C R | | 0,,1: | 1 |
| 16 | Soil nitrogen loss as a result of alternate sub- mergence and drying显示文摘 | Patrick W H Wyatt R | 1964 | Soil Sci Soc Am J1964,28,5: | 1 |
| 17 | Pain and ethnicity 显示文摘 | Wyatt R | 2013 | Virtual Mentor2013,15,5: | 1 |
| 18 | Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis显示文摘 | Kapikian AZ Wyatt RG Dolin R | 1972 | J Virol1972,10,: | 1 |
| 19 | Patients with IgA nephropathy have increased serum galactose- deficient IgA1 levels显示文摘 | Moldoveanu Z Wyatt R J Lee JY | 2007 | Kidney lnt2007,71,11: | 1 |
| 20 | Probability analysis of variational crystallization and its application to gp120,the exterior enveloope glycoprotein of type 1 human immunideficiency virus (HIV-1) 显示文摘 | Wyatt R Desjardins E | 1999 | J Biol Chem1999,274,: | 1 |