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| 1 | Effect of nitrogen fertilization on yield, N content, and nitrogen fixation of alfalfa and smooth bromegrass grown alone or in mixture in greenhouse pots显示文摘Planting grass and legume mixtures on improved grasslands has the potential advantage of realizing both higher yields and lower environmental pollution by optimizing the balance between applied N fertilizer and the natural process of legume biological nitrogen fixation. However, the optimal level of N fertilization for grass-legume mixtures, to obtain the highest yield, quality, and contribution of N2 fixation, varies with species. A greenhouse pot experiment was conducted to study the temporal dynamics of N2 fixation of alfalfa(Medicago sativa L.) grown alone and in mixture with smooth bromegrass(Bromus inermis Leyss.) in response to the addition of fertilizer N. Three levels of N(0, 75, and 150 kg ha–1) were examined using 15N-labeled urea to evaluate N2 fixation via the 15 N isotope dilution method. Treatments were designated N0(0.001 g per pot), N75(1.07 g per pot) and N150(2.14 g per pot). Alfalfa grown alone did not benefit from the addition of fertilizer N; dry matter was not significantly increased. In contrast, dry weight and N content of smooth bromegrass grown alone was increased significantly by N application. When grown as a mixture, smooth bromegrass biomass was increased significantly by N application, resulted in a decrease in alfalfa biomass. In addition, individual alfalfa plant dry weight(shoots+roots) was significantly lower in the mixture than when grown alone at all N levels. Smooth bromegrass shoot and root dry weight were significantly higher when grown with alfalfa than when grown alone, regardless of N application level. When grown alone, alfalfa's N2 fixation was reduced with N fertilization(R2=0.9376, P=0.0057). When grown in a mixture with smooth bromegrass, with 75 kg ha–1 of N fertilizer, the percentage of atmospheric N2 fixation contribution to total N in alfalfa(%Ndfa) had a maximum of 84.07 and 83.05% in the 2nd and 3rd harvests, respectively. Total 3-harvest %Ndfa was higher when alfalfa was grown in a mixture than when grown alone(shoots: |t|=3.39, P=0.0096; root: |t|=3.57, P=0.0073). We believe this was due to smooth bromegrass being better able to absorb available soil N(due to its fibrous root system), resulting inlower soil N availability and allowing alfalfa to develop an effective N2 fixing symbiosis prior to the 1st harvest. Once soil N levels were depleted, alfalfa was able to fix N2, resulting in the majority of its tissue N being derived from biological nitrogen fixation(BNF) in the 2nd and 3rd harvests. When grown in a mixture, with added N, alfalfa established an effective symbiosis earlier than when grown alone; in monoculture BNF did not contribute a significant portion of plant N in the N75 and N150 treatments, whereas in the mixture, BNF contributed 17.90 and 16.28% for these treatments respectively. Alfalfa has a higher BNF efficiency when grown in a mixture, initiating BNF earlier, and having higher N2 fixation due to less inhibition by soil-available N. For the greatest N-use-efficiency and sustainable production, grass-legume mixtures are recommended for improving grasslands, using a moderate amount of N fertilizer(75 kg N ha–1) to provide optimum benefits. | XIE Kai-yun LI Xiang-lin HE Feng ZHANG Ying-jun WAN Li-qiang David B Hannaway WANG Dong QIN Yan Gamal M A Fadul | 2015 | Journal of Integrative Agriculture2015,14,9: | 5 |
| 2 | Physical properties of dewatered wastewater sludge for landfilling 显示文摘 | Koening A Kay J N Wan I M | 1996 | War Sci Tech1996,34,34: | 2 |
| 3 | Clinical measures of disease severity and outcome in psoriasis:a critical appraisal of their quality显示文摘 | ASHCROFT D M WAN PO A L WILLIAMS H C | 1999 | Br J Dermatol1999,141,2: | 1 |
| 4 | Delay-independent criteria for exponential stability generalized Cohen- Grossberg neural networks with discrete delay显示文摘 | Wan A H Qiao H Peng J G Wang M S | 2006 | Physics Letters A2006,353,: | 1 |
| 5 | The Unscented Kalman Filter for Nonlinear Estimation 显示文摘 | Wan E A Rudolph V M | 2000 | Adaptive Systems for Signal Processing Communication and Control IEEE2000,45,3: | 1 |
| 6 | Adsorption of humic acid from aqueous solutions on crosslinked chi- tosan-epichlorohydrin beads: kinetics and isotherm stud- ies显示文摘 | Wan Ngah W S Hanafiah M A Yong S S | 2008 | Colloids and Surfaces B: Biointerfaces2008,65,: | 1 |
| 7 | Grassland responses to grazing:effects of grazing intensity and management system in an Inner Mongolian steppe ecosystem显示文摘 | Sch(o)nbach P Wan HW Gierus M Bai YF Müller K Lin LJ Susenbeth A Taube F | | 0,,: | 1 |
| 8 | Roles of coactosin-like protein (CLP) and 5-1ipoxygenase-activating protein (FLAP) in cellular leukotriene biosynthesis显示文摘 | Basavarajappa D Wan M Lukic A | 2014 | Proc Nail Acad Sci U S A2014,111,11: | 1 |
| 9 | Distributed construction of connected dominating sets in wireless ad hoc networks显示文摘 | WAN P J KHALED M A OPHIR F | 2004 | ACM/Kluwer Mobile Networks and Applications2004,9,2: | 1 |
| 10 | Adsorption ofdyes and heavy metal ions by chitosan composites:A review显示文摘 | Wan Ngah Teong L C Hanafiah M A K M | 2011 | Carbohydr Polym2011,83,4: | 1 |
| 11 | Removal of heavy metal ions from wastewater by chemically modified plant wastes as adsorbents: Areview 显示文摘 | Wan Ngah W S Hanafiah M A K M | 2008 | Bioresource Technology2008,99,10: | 1 |
| 12 | Wheat stripe rust in China 显示文摘 | Wan A M Chen X M He Z H | 2007 | Australian Journal of Agricultural Research2007,58,6: | 1 |
| 13 | Influence of aging days and age at harvest on meat quality of Gannan Black yak显示文摘 | Wan H L Zhang L P Brown M A | 2011 | J Anim and Vet Adv2011,10,9: | 1 |
| 14 | Wheat stripe rust in China显示文摘 | Wan A M Chen X M He Z H | 2007 | Austrulian Journal of Agricultural Research2007,58,6: | 1 |
| 15 | The HgCdTe Electron Avalanche Photodiode 显示文摘 | Beck J D Wan C Kinch M A | 2006 | Journal of Elec- tronic Material2006,35,6: | 1 |
| 16 | The effect of mixotrophy on microalgal growth, lipid content,and expression levels of three pathway genes in Chlorella sorokiniana 显示文摘 | WAN MX LIU P XIA J L ROSENBERG J N OYLER G A BETENBAUGH M J NIE Z Y QIU G Z | 2011 | Applied Microbiology and Biotechnology2011,91,3: | 1 |
| 17 | Wheat stripe rust in China显示文摘 | Wan A M Chen X M He Z H | | 0,,: | 1 |
| 18 | Brassinosteroids regulate grain filling in rice 显示文摘 | Wu C Y Trleu A Radhakrishnan P Kwok S F Harris S Zhang K Wang J L Wan J M Zhai H Q Takatsuto S Matsumoto S Fujioka S Feldmann K A Pennell R I | 2008 | Plant Cell2008,20,8: | 1 |
| 19 | Natural (^210Pb, ^7Be) and fallout (^137Cs,^239,240pu,^9OSr) radionuclides as geochemical tracers of sedimentation in Greifensee, Switzerland 显示文摘 | Wan G J Santsehi P H Sturm M Farrenkothen K Lueck A Werth W Schuler Ch | 1987 | Chem Geol1987,63,34: | 1 |
| 20 | Leukotriene B4-induced changes in vascular permeability are mediatedby neutrophil release of heparin-binding protein (HBP/CAP37/azurocidin) 显示文摘 | Di Gennaro A Kenne E Wan M | 2009 | FASEB J2009,23,6: | 1 |