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| 1 | 光敏剂间-四羟基苯二氢卟酚及其复合物对人T淋巴细胞白血病细胞的作用显示文摘目的 观察光敏剂间-四羟基苯二氢卟酚(mTHPC)及其复合物HSA-mTHPC对人T淋巴细胞白血病细胞(Jurkat)的作用,探讨人血白蛋白(HSA)纳米颗粒作为mTHPC载体的可行性.方法 利用流式细胞仪测定Jurkat细胞对mTHPC及HSA-mTHPC的摄取率,通过测定不同浓度mTHPC及HSA-mTHPC孵育Jurkat细胞后的存活率、细胞膜完整性和增殖率,评价两种药物的摄取率和细胞毒性.结果 浓度1.0 mg/L,孵育4、24 h Jurkat细胞的摄取率为:mTHPC单体:98.07%、99.07% HSA-mTHPG中mTHPC:98.13%、99.19% HSA单体:2.77%、4.84%.光照后Jurkat细胞活性和增殖率呈显著剂量、时间依赖的下降,mTHPC浓度为1.0 mg/L时,4、24、48 h存活率和增殖率分别为13.27%、5.46%、4.99%和2.68%、0.91%、0.62%,HSA-mTHPC对细胞生长和增殖的抑制与mTHPC单体比较未见下降(P>0.05).光照后LDH活性呈现显著剂量、时间依赖的上升,mTHPC浓度为1.0mg/L时,4、24、48 h LDH活性分别为:80.99%、99.38%、100.67%.结论 mTHPC是一种杀伤力强的光敏剂型,能有效进入肿瘤细胞,并导致细胞损害.HSA纳米颗粒不影响细胞对mTHPC的摄取,可以作为其载体. | 郑顺贞 Karin Low Matthis Wacker Arno Wiehe Sylvia Wagner Klaus Langer Hagen von Briesen 邹声泉 | 2010 | 中华实验外科杂志2010，27，10： | 2 |
| 2 | Membrane properties change in fine-pore aeration diffusers: Full-scale variations of transfer efficiency and headloss显示文摘 | Diego Rosso Judy A Libra Wolfgang Wiehe | 2008 | Water Research2008，42，10： | 1 |
| 3 | Absence of asso- ciation between depression and hypertension : results of a prospec- tively designed population - based study 显示文摘 | WIEHE M FUCHS S C MOREIRA L B | 2006 | J Hum Hypertens2006，30，6： | 1 |
| 4 | A phase-separation kinetic model for coke formation显示文摘 | WIEHE I A | 1993 | Ind Eng Chem Rev1993，32，11： | 1 |
| 5 | The oil compatibility model and crude oil incompatibility显示文摘 | WIEHE I A KENNEDY R J | 2000 | Energy Fuels2000，14，1： | 1 |
| 6 | Studies on the vegetation of Mauritius显示文摘 | VAUGHAN R E WIEHE P O | 1937 | J of Ecology1937，25，： | 1 |
| 7 | The oil compatibility model and crude oil incompatibility 显示文摘 | Wiehe I A Kennedy R J | 2000 | Energy & Fuels2000，14，1： | 1 |
| 8 | Calcium phosphate nano particles as efficient carriers for photodynamic therapy against cells and bacteria显示文摘 | Schwiertz J Wiehe A Grafe S | 2009 | Biomaterials2009，30，19： | 1 |
| 9 | A phase-separation kinetic model for coke formation显示文摘 | WIEHE I E | 1993 | Ind Eng Chem Res1993，32，11： | 1 |
| 10 | Die ontogenese der salzdrüsen von Limonium (Plumbaginaceae)显示文摘 | WIEHE W BRECKLE S W | 1990 | Botanica Acta1990，103，： | 1 |
| 11 | Design of synthetic dispersants for asphaltenes显示文摘 | Wiehe I A Jermansen T G | 2003 | Petroleum Science and Technology2003，21，3： | 1 |
| 12 | Role of plectin in cytoskeleton organization and dynam?ics显示文摘 | Wiehe G | 1998 | J Cell Sci1998，111，17： | 1 |
| 13 | Absence of association between depression and hypertension:results of a prospectively designed population-based study显示文摘 | Wiehe M Fuchs SC Moreira LB | 2006 | J Hum Hypertens2006，20，6： | 1 |
| 14 | Design of synthetic dispersants for asphaltenes 显示文摘 | WIEHE I A JERMANSEN T G | 2003 | Petroleum Science and Technology2003，21，9： | 1 |
| 15 | A phase-separation kinetic model for coke formation显示文摘 | WIEHE I A | 1993 | Ind Eng Chem Res1993，32，11： | 1 |
| 16 | Design of syntheticdispersants for asphaltenes 显示文摘 | WIEHE I A JERMANSEN T G | 2003 | Petroleum Science andTechnology2003，21，34： | 1 |
| 17 | mRNA-mediated gene delivery into human progenitor cells promotes highly efficient protein expression 显示文摘 | Wiehe JM Ponsaerts P Rojewski MT | 2007 | J Cell Mol Med2007，11，3： | 1 |
| 18 | Survival of plant growth promoting rhizosphere bacteria in the rhizosphere of different crops and migration to no-inoculated plants under field conditions in north-east Germany显示文摘 | WIEHE W HOFLICH G | 1995 | Microbiology Research1995，150，： | 1 |
| 19 | Comparative sequence analysis of the MECP2-locus in human and mouse reveals new transcribed regions显示文摘 | Reichwald K Thiesen J Wiehe T | 2000 | Mammalian Genome2000，11，： | 1 |
| 20 | A phase - separation kinetic model for coke formation显示文摘 | WIEHE I A | 1993 | Ind Eng Chem Res1993，32，11： | 1 |
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