|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Key factors in developing the trinitrobenzene sulfonic acid-induced post-inflammatory irritable bowel syndrome model in rats显示文摘AIM:To investigate the key factors in developing the trinitrobenzene sulfonic acid(TNBS)-induced postinflammatory irritable bowel syndrome(PI-IBS)model in rats. METHODS:TNBS was administered to rats at the following conditions:(1)with different doses(20,10,5 mg/0.8 mL per rat);(2)with same dose in different concentrations(20 mg/rat,25,50 mg/mL);(3)in different ethanol percentage(25%,50%);and(4)at depth either 4 cm or 8 cm from anus.At 5 d and 4 wk after TNBS administration,inflammation severity and inflammation resolution were evaluated.At 4 and 8 wk after TNBS application,visceral hyperalgesia and enterochromaffin(EC)cell hyperplasia were assayed by abdominal withdrawal reflex test,silver staining and capillary electrophoresis. RESULTS:Our results showed that:(1)TNBS induced dose-dependent acute inflammation and inflammation resolution.At 5 d post TNBS,the pathological score and myeloperoxidase(MPO)activity in all TNBS treated rats were significantly elevated compared to that of the control(9.48±1.86,8.18±0.67,5.78± 0.77 vs 0,and 3.55±1.11,1.80±0.82,0.97±0.08 unit/mg vs 0.14±0.01 unit/mg,P<0.05).At 4 wk post TNBS,the pathological score in high and median dose TNBS-treated rats were still significantly higher than that of the control(1.52±0.38 and 0.80±0.35 vs 0,P<0.05);(2)Intracolonic TNBS administration position affected the persistence of visceral hyperalgesia.At 4 wk post TNBS,abdominal withdrawal reflex (AWR)threshold pressure in all TNBS-treated groups were decreased compared to that of the control(21.52 ±1.73 and 27.10±1.94 mmHg vs 34.44±1.89 mmHg,P<0.05).At 8 wk post TNBS,AWR threshold pressure in 8 cm administration group was still significantly decreased(23.33±1.33 mmHg vs 36.79±2.29 mmHg,P<0.05);(3)Ethanol percentage affected the TNBS-induced inflammation severity and visceral hyperalgesia.In TNBS-25%ethanol-treated group,the pathological score and MPO activity were significantly lowered compared to that of the TNBS-50%ethanoltreated group,while AWR threshold pressure were significantly elevated(36.33±0.61 mmHg vs 23.33±1.33 mmHg,P<0.05);and(4)TNBS(5 mg/0.8 mL per rat, in 50%ethanol,8 cm from anus)-treated rats recovered completely from the inflammation with acquired visceral hyperalgesia and EC cell hyperplasia at 4 wk after TNBS administration.CONCLUSION:TNBS dosage,concentration,intraco-lonic administration position,and ethanol percentage play important roles in developing visceral hyperalgesia and EC cell hyperplasia of TNBS-induced PI-IBS rats. | Hong-Yan Qin Hai-Tao Xiao Justin CY Wu Brian M Berman Joseph JY Sung Zhao-Xiang Bian | 2012 | World Journal of Gastroenterology2012，18，20： | 10 |
| 2 | Nitrite-derived nitric oxide by xanthine oxidoreductase protects the liver against ischemia- reperfusion injury显示文摘It was demonstrated that xanthine oxidoreductase (XOR), during ischemia, catalyzes the formation of nitric oxide (NO) from nitrite (NO2-) and this NO2--derived NO protects the isolated perfused rat heart against the damaging effects of ischemia-reperfusion (I/R) when conventional nitric oxide synthase (NOS) -dependent NO production is impaired. Liver is one of the organs with the highest XOR concentration. This study was designed to determine whether NO2--derived NO by XOR protects liver against I/R injury in vivo. For its minute amounts and active reactivity, NO can not be detected directly in real time in vivo by this time. We have to prove the above hypothesis indirectly. METHODS:Wistar rats were pretreated with saline, NOS inhibitor L-NAME (10 mg/kg intravenously), XOR inhibitor allopurinol (1.5 mg/kg orally), L-NAME +allopurinol and NO scavenger carboxy-PTIO (0.6 mg/kg intravenously) respectively (12 animals per group). And then, they were subjected to total liver ischemia for 40 minutes followed by reperfusion. Blood samples and liver tissues were obtained for analysis after 3 hours of reperfusion. Survival was also investigated. RESULTS:Allopurinol-treated animals exhibited further increased serum alanine aminotransferase (ALT) levels and liver myeloperoxidase (MPO) activities, but further decreased liver adenosine triphosphate (ATP) stores after I/R compared to saline-treated counterparts (830.5±108.3 U/L, 56.5±11.0 U/mg protein and 1.93±0.47 μmol/g vs. 505.8± 184.2 U/L, 41.5±10.2 U/mg protein and 3.05±0.55 μmol/g respectively, P<0.01, P<0.05 and P<0.01 respectively). The hepatocyte injury was further exacerbated and the overall survival rate was significantly decreased after I/R in animals given by allopurinol compared to those pretreated by saline (P<0.05). L-NAME and allopurinol co-treated animals exhibited more severe liver injury (P<0.05 and P<0.01) and a further decreased overall survival rate (P<0.05) compared to L-NAME or allopurinol alone-treated counterparts, but they were not different from carboxy-PTIO treated animals (P>0.05). CONCLUSION:NO2--derived NO by XOR in the hypoxic and acidic environment induced by hepatic I/R protects the liver against I/R injury in vivo. | Department of General Surgery ( Lu P, Wang CY and Chen DD), and Department of Radiology (Liu F), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China Cold Spring Harbor Laboratory, New York 11724, USA ( Yao Z) General Surgery Laboratory, Union Hospital, Tongji Medical College , Huazhong University of Science and Technology, Wuhan 430022 , China (Tian Y, Zhang JH and Wu YH) | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 4 |
| 3 | Genetic polymorphism in pathogenesis of irritable bowel syndrome显示文摘Irritable bowel syndrome(IBS)is a complex symptombased disorder without established biomarkers or putative pathophysiology.IBS is a common functional gastrointestinal disorder which is defined as recurrent abdominal pain or discomfort that has at least two of the following symptoms for 3 d per month in the past 3mo according to ROMEⅢ:relief by defecation,onset associated with a change in stool frequency or onset with change in appearance or form of stool.Recent discoveries revealed genetic polymorphisms in specific cytokines and neuropeptides may possibly influence the frequencies and severity of symptoms,as well as the therapeutic responses in treating IBS patients.This review gives new insights on how genetic determinations influence in clinical manifestations,treatment responses and potential biomarkers of IBS. | Cynthia KY Cheung Justin CY Wu | 2014 | World Journal of Gastroenterology2014，20，47： | 3 |
| 4 | 由磁共振成像评估的主动脉弓脉搏波传导速度是心血管事件的预测因子:多种族动脉粥样硬化研究显示文摘通过磁共振成像评估的主动脉弓脉搏波传导速度(pulse wave velocity,PWV)对心血管病事件的预测价值尚未完全确定。为了评估多种族动脉粥样硬化研究(multi-ethnic study of atherosclerosis,MESA)受试者中主动脉弓PWV与心血管病的关系,Ohyama等纳入3527例MESA受试者[基线时平均年龄为(62±10)岁,47%为男性], | 刘莉 叶鹏 Ohyama Y Ambale-Venkatesh B Noda C Kim JY Tanami Y Teixido-Tura G Chugh AR Redheuil A Liu CY Wu CO Hundley WG Bluemke DA Guallar E Lima JAC | 2017 | 中华高血压杂志2017，25，8： | 2 |
| 5 | NKCC1-mediated traumatic brain injury-induced brain edema and neuron death via Raf/MEK/MAPK cascade显示文摘 | Lu KT Cheng NC Wu CY | 2008 | Crit Care Med2008，36，3： | 1 |
| 6 | Vertebral fracture assessment using a semiquantitative technique显示文摘 | Genant HK Wu CY van Kuijk C | 1993 | J Bone Miner Res1993，8，9： | 1 |
| 7 | Cytokine regulation of IL 12 receptor β2 expression: differential effects on human T and NK cells显示文摘 | Wu CY Gadina M Wang K | 2000 | Eur J Immunol2000，30，5： | 1 |
| 8 | High frequency of linezolid-associated thrombocytopenia and anemia among patients with end- stage renal disease显示文摘 | Wu VC Wang YT Wang CY | 2006 | Clin Infect Dis2006，42，1： | 1 |
| 9 | Vertebral fracture assenss- ment using a semiquantitative technique显示文摘 | Genant HK Wu CY van Kuijk C | 1993 | J Bone Miner Res1993，8，9： | 1 |
| 10 | Mechanism of macrophage migration inhibitory factor-induced decrease of T-type Ca^2+ channel current in atrium-derived cells显示文摘 | Rao F Deng CY Wu SL | 2013 | Exp Physiol2013，98，1： | 1 |
| 11 | Muir-Torre syndrome: extraocular sebaceous carcinoma with adenocarcinoma of colon in a 76-year-old man显示文摘 | Wu CY | 2009 | Clin Exp Dennatol2009，34，39： | 1 |
| 12 | MicroRNA-223 regulates FOXO1 expression and cell proliferation显示文摘 | Wu L Li H Jia CY | 2012 | FEBS Lett2012，586，7： | 1 |
| 13 | Glutathione S-transferase M1, T1, and P1 polymorphisms as susceptibility factors for noise-induced temporary threshold shift显示文摘 | Lin CY Wu JL Shih TS | 2009 | Hearring Res2009，815，： | 1 |
| 14 | Reliability, validity, and re- sponsiveness of Myotonometric measurement of muscle tone,elasticity, and stiffness in patients with stroke显示文摘 | Chuang LL Wu CY Lin KC | 2012 | Archives of Physical Medicine and Rehabilitation2012，93，3： | 1 |
| 15 | Evaluation of Physical Capture Efficiency and Disinfection Capability of an lodinated Biocidal Filter Medium显示文摘 | Shumate SR Wu CY wander J | | 0，，01： | 1 |
| 16 | Imaging evaluation of meniscal injury of the knee joint:a comparative MR imaging and arthroscopic study显示文摘 | Chang CY Wu HT Huang TF | 2004 | Cliu Imaging2004，28，5： | 1 |
| 17 | Cigarette smoking and the risk of colorectal cancer: a meta-analysis of prospective cohort studies 显示文摘 | Tsoi KK Pan CY Wu WK | 2009 | Clin Gastroenterol Hepatol2009，7，6： | 1 |
| 18 | Prenatal 2D ultrasonic diagnosis offetal complete atrioventricular block and bilateral hydrocelea withaccuhmaternal SSA显示文摘 | Chen CY Wu YC Yang ML | 2006 | Taiwan J Obstet Gyneco2006，45，1： | 1 |
| 19 | Flow behavior of powders during die filling 显示文摘 | Wu CY Cocks ACF | 2004 | Powder Metall2004，47，2： | 1 |
| 20 | Amyloidosis cutis dyschromica: four cases from two families显示文摘 | Huang WH Wu CY Yu CP | 2009 | Int J Dermatol2009，48，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员