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| 1 | Survival of transplanted neurotrophin-3 expressing human neural stem cells and motor function in a rat model of spinal cord injury显示文摘<正>BACKGROUND:Many methods have been attempted to repair nerves following spinal cord injury, including peripheral nerve transplantation,Schwann cell transplantation,olfactory ensheathing cell transplantation,and embryonic neural tissue transplantation.However,there is a need for improved outcomes. OBJECTIVE:To investigate the repair feasibility for rat spinal cord injury using human neural stem cells(hNSCs) genetically modified by lentivirus to express neurotrophin-3. DESIGN,TIME AND SETTING:In vitro cell biological experiment and in vivo randomized,controlled, genetic engineering experiment were performed at the Third Military Medical University of Chinese PLA and First People's Hospital of Yibin,China from March 2006 to December 2007. MATERIALS:A total of 64 adult,female,Wistar rats were used for the in vivo study.Of them,48 rats were used to establish models of spinal cord hemisection,and were subsequently equally and randomly assigned to model,genetically modified hNSC,and normal hNSC groups.The remaining 16 rats served as normal controls. METHODS:hNSCs were in vitro genetically modified by lentivirus to secrete both green fluorescence protein and neurotrophin-3.Neurotrophin-3 expression was measured by Western blot. Genetically modified hNSC or normal hNSC suspension(5×10~5) was injected into the rat spinal cord following T_(10) spinal cord hemisection.A total of 5μL Dulbecco's-modified Eagle's medium was infused into the rat spinal cord in the model group.Transgene expression and survival of transplanted hNSCs were determined by immunohistochemistry.Motor function was evaluated using the Basso,Beattie,and Bresnahan(BBB) scale. MAIN OUTCOME MEASURES:The following parameters were measured:expression of neurotrophin-3 produced by genetically modified hNSCs,transgene expression and survival of hNSCs in rats,motor function in rats. RESULTS:hNSCs were successfully genetically modified by lentivirus to stably express neurotrophin-3.The transplanted hNSCs primarily gathered at,or around,the injection site two weeks following transplantation,and gradually migrated towards the surrounding tissue. Transplanted hNSCs were observed 7.0-8.0 mm away from the injection site.In addition,hNSCs were observed 10 weeks after transplantation.At week 4,BBB locomotor scores were significantly greater in the genetically modified hNSC and normal hNSC groups,compared with the model group (P<0.05),and scores were significantly greater in the genetically modified hNSC group compared with the normal hNSC group(P<0.05). CONCLUSION:hNSCs were genetically modified with lentivirus to stably secrete neurotrophin-3. hNSCs improved motor function recovery in rats following spinal cord injury. | Peiqiang Cai Guangyun Sun Peishu Cai Martin Oudega Rui Xiao Xuewen Wang Wei Li Yunbing Shu Cheng Cai Haihao Yang Xuebing Shan Wuhua Luo | 2009 | Neural Regeneration Research2009，4，7： | 18 |
| 2 | Expert Recommendations for Tracheal Intubation in Critically Ill Patients with Noval Coronavirus Disease 2019显示文摘Coronavirus Disease 2019(COVID-19),caused by a novel coronavirus(SARS-Co V-2),is a highly contagious disease.It firstly appeared in Wuhan,Hubei province of China in December 2019.During the next two months,it moved rapidly throughout China.Most of the infected patients have mild symptoms including fever,fatigue and cough,but in severe cases,patients can progress rapidly and develop into acute respiratory distress syndrome,septic shock,metabolic acidosis and coagulopathy.The new coronavirus was reported to spread via droplets,contact and natural aerosols from human to human.Therefore,aerosol-producing procedures such as endotracheal intubation may put the anesthesiologists at high risk of nosocomial infections.In fact,SARSCo V-2 infection of anesthesiologists after endotracheal intubation for confirmed COVID-19 patients have been reported in hospitals in Wuhan.The expert panel of airway management in Chinese Society of Anaesthesiology has deliberated and drafted this recommendation,by which we hope to guide the performance of endotracheal intubation by frontline anesthesiologists and critical care physicians.During the airway management,enhanced droplet/airborne personal protective equitment(PPE)should be applied to the health care providers.A good airway assessment before airway intervention is of vital importance.For patients with normal airway,awake intubation should be avoided,and modified rapid sequence induction is strongly recommended.Sufficient muscle relaxant should be assured before intubation.For patients with difficult airway,good preparation of airway devices and detailed intubation plans should be made. | Mingzhang Zuo Yuguang Huang Wuhua Ma Zhanggang Xue Jiaqiang Zhang Yahong Gong Lu Che 无 | 2020 | Chinese Medical Sciences Journal2020，35，2： | 7 |
| 3 | AN EXPERIMENTAL STUDY OF SHEET EROSION IN GRANITIC REGION OF WUHUA COUNTY,GUANGDONG PROVINCE显示文摘The relationships between rainfall, runoff and erosion in South China granitic weathering red soil region were studied by using data collected from 7 experimental plots in Wuhua County, Guangdong Province. Results showed that when rainfall is less than 50 mm, small volumes of runoff were produced. Rainfall intensity might be the main factor in controlling runoff. When rainfall exceeds 50 mm, because of its large volume and high intensity, runoff and the produced amount of erosion increased with raimfall quickly. Analysis of the relationship bebeen runoff and erosion suggsted that runoff of 30 mm (corresponding to about 50 mm rainfall might be taken as a threshold at which wter on land surface was sufficient to start the larg amount of soil particles. Vegtation cover is very effective in protection soil from being eroded. When vegetation coverag increases from 0 to 80 percent,runoff and soil loss decrease from 179.34mm/a to 126.36mm/a and 6262.15T/a km2 to 368.02 T/a km2, respectively. | Jin Changxing(Institute of Geography, CAS, Bejing 100101 people’s Republic of China)Zang Guohua(Soil and Water Conservation Experimental Station of Wuhua, Guangdong 514471People’s Republic of China) | 1994 | Journal of Geographical Sciences1994，4，Z2： | 2 |
| 4 | BOUNDARY ELEMENT METHOD FOR CALCULATING ACOUSTIC RADIATION FROM CLOSED SURFACE WITH PRESCRIBED VELOCITY DISTRIBUTION显示文摘The application of BEM to calculate acoustic radiation from closed surface in an infinite acoustic me-dium has the advantages of less memory space,higher accuracy and faster calculating speed.However,the singular integrals and the nonuniqueness problem of surface helmholtz equation at characteristic frequen-cies should be dealt with.It is suggested in this paper that combination of the interior Helmholtz equationand its derivatives with respect to the coordinate components of an interior point forms an extra equation,in order to solve the problems of acoustic radiation at various frequencies with surface Helmholtz equation;on the area which includes singular point,the singular integrals are converted to ordinary ones by polarcoordinate transformation.As an example.the acoustic radiation field of closed axisymmetric surfaces withprescribed surface velocity distribution is calculated in this paper. | Zhao, Jian Wang, Hongzhen Zhu, Wuhua | 1989 | Chinese Journal of Acoustics1989，8，2： | 2 |
| 5 | ANALYSIS OF FREEMIUM BUSINESS MODEL CONSIDERING NETWORK EXTERNALITIES AND CONSUMER UNCERTAINTY显示文摘公司逐渐地在联机软件市场使用的一个新兴的企业模型将两个都为为顾客的一种服务或一个产品提供一个免费基本版本和一个付的高级版本。如此的一个背景经常被称为 freemium 模型。免费版本的存在能关于商业软件的评估减少顾客无常并且使用网络效果改进公司利润。然而, freemium 模型可以也让 cannibalization 完成哪个罐头伤害了利润。因此,公司需要为免费版本和最佳的价格决定最佳的内容让高级版本最大化它的利润。在这份报纸,首先,我们获得 freemium 模型和他们的性质的最佳的决定。第二,我们把 freemium 模型与产品满意的所有需要以利润,顾客福利,和社会福利被收的传统的 charge-for-everything 模型作比较。结果证明当顾客显著地低估软件的值,软件的真值足够高时, freemium 模型能产生更高的利润,更高级的顾客福利,和更高社会的福利。否则, freemium 模型不能交付需要的结果。 | Wuhua Chen Zhongsheng Hua Zhe George Zhang Wenjie Bi | 2018 | Journal of Systems Science and Systems Engineering2018，27，1： | 2 |
| 6 | The Partial Purification of Angiogenesis Factor of Human Osteosarcoma显示文摘Objective To partially purify the angiogenesis factor of human osteosarcoma(HuOs) and study its biological features. Methods The active peptide with a molecular weight of 8000-10000 Da in the conditioned medium obtained from the cultivation of Hu-Os cells(osteoblastic osteosarcoma) was partially purified by ultrafiltration, chromatography and dialysis.The angiogenic effects of the fractions were assessed by proliferation assay of human umbilical vein and pig thoracic aorta endothelial cells. Results The chromatography fractions 4-6 could significantly promote the proliferation of the endothelial cells.Conclusion The HuOs cells could synthesize and secrete angiogenesis factor with a molecular weight of 8000-10000 Da. | WUHua DENGZhongduan 等 | 2002 | The Chinese-German Journal of Clinical Oncology2002，1，2： | 2 |
| 7 | The interaction o{ waxes with pour point de pressants显示文摘 | Chen Wuhua | 2010 | Fuel2010，89，5： | 1 |
| 8 | Generalderivation law of nonisolated high-step-up interleavedconverters with built-in transformer显示文摘 | Li Wuhua Li Weichen He Xiangning | 2012 | IEEETransactions on Industrial Electronics2012，59，3： | 1 |
| 9 | Design and Analysis of a Grid-Connected Photovoltaic Power System显示文摘 | Bo Y Wuhua L Yi Z | 2010 | IEEE Transactions on Power Electronics2010，25，4： | 1 |
| 10 | Review of nonisolated high-step-up DC/DC converters in photovohaic grid-connected applications显示文摘 | LI Wuhua HE Xiangning | 2011 | IEEE Transactions on Power Electronics2011，58，4： | 1 |
| 11 | Reliability analysis of shearing machine feedingsystem 显示文摘 | YANGHongyue(杨宏悦) WUHua(吴华) OUYANGLihua(欧阳立华) etal | 2010 | 核标准计最与质摄2010，，1： | 1 |
| 12 | Development of a 420 mm annular centrifugal contactor for the hot test of the total TRPO process显示文摘 | DUAN Wuhua CHENG Qin | 2009 | Progress in Nuclear Energy2009，51，： | 1 |
| 13 | Influence of human mutant p27 gene on the biological behaviors of colon cancer cells显示文摘目的将在结肠癌细胞的生长和 apoptosis 上观察人的变异的 p27 基因(p27mt ) 的影响以便为结肠癌在基因治疗调查 p27mt 的功能机制。方法结肠癌房间线 SW480 感染 recombinant 复制有缺陷者侵入人体气管粘膜的病菌 Ad-p27mt,并且 p27mt 蛋白质的表示被西方的污点检测;SW480 房间上的 p27mt 的抑制效果与血细胞计数被检测。房间周期与流动血细胞计数器被决定,并且 DNA 碎裂分析过程识别了 apoptosis 的出现。在有 Ad-p27mt 的 transfected SW480 房间以后的结果, p27 蛋白质的高表示与免疫弄污被识别试金。染色的 PI 和流动血细胞计数器试金证明当在 Ad-LacZ 组和空白控制组时, 77.96% 结肠癌房间在阶段 G 0/G1, 被堵住, 27.57% 和 25.29% 房间分别地在一样的阶段被堵住。生长曲线证明 Ad-p27mt 在 SW480 房间的生长上有明显的抑制效果, DNA 碎片试金证明 p27mt 能导致结肠癌房间的 apoptosis。结论 p27mt 在结肠癌房间周期,和大多数房间上有明显的堵住效果在阶段 G 0/G1 被堵住。这阻塞与生长抑制和 p27mt 的 apoptosis 正式就职效果被联系。 | Jun Chen Guangxin Lu Bin Wang Wuhua Ding | 2009 | The Chinese-German Journal of Clinical Oncology2009，8，1： | 1 |
| 14 | Review of nonisolated high-step-up DC/DC converters in photovoltaic grid-connected applications显示文摘 | Li Wuhua He Xiangning | 2011 | IEEE Transactions on Power Electronics2011，58，4： | 1 |
| 15 | The interaction of waxes with pour point depressants显示文摘 | CHEN Wuhua ZHAO Zongchang YIN Caoyong | 2010 | Fuel2010，89，5： | 1 |
| 16 | H∞ control of uncertain linear impulsive stochastic systems显示文摘 | CHEN Wuhua WANG Junge TANG Youjian LU Xiaomei | 2008 | International Journal of Robust and Nonlinear Control2008，18，13： | 1 |
| 17 | General derivation law of nonisolated high step-up interleaved converters with builtin transformer显示文摘 | Wuhua Li Weichen Li Xiangning He | 2012 | IEEE Transactions on Industrial Electronics2012，59，3： | 1 |
| 18 | 显示文摘 | XUYong-qiang PENGWei-cheng WUHua(许永强 彭伟成 武华) | | 物理学报0，，： | 1 |
| 19 | A new method for complete stability analysis of of cellular neural networks with time delay 显示文摘 | Chen Wuhua Zheng Weixing | 2010 | IEEE Transactions on Neural Networks2010，21，7： | 1 |
| 20 | The interaction of waxes with pour point depressant显示文摘 | Wuhua Chen Zongchang Zhao Caoyong Yin | 2010 | Fuel2010，，89： | 1 |
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