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| 1 | Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system显示文摘允许的技术内长的基因的特定的规定为基因功能的学习是珍贵的并且在治疗学有大潜力。我们创造了在 CRISPR 上系统,由核酸酶死者 Cas9 (dCas9 ) 蛋白质组成的二部件的 transcriptional 使活跃之物与互补顺序与 transcriptional 激活域和单个指南 RNA (sgRNAs ) 熔化了到基因倡导者。我们证明在 CRISPR 上能高效地以一种悦耳的方式在人和老鼠房间激活外长的记者基因。另外,我们证明在 vivo 的柔韧的记者基因激活能被把系统部件注入老鼠接合子完成。而且,我们证明在 CRISPR 上能激活内长的 IL1RN, SOX2,和 OCT4 基因。最有效的基因激活被对近似倡导者有约束力的 3-4 sgRNAs 的簇完成,建议他们在基因正式就职的 synergistic 行动。显著地,当指向多重基因的 sgRNAs 同时被介绍进房间时,柔韧的多路的内长的基因激活被完成。染色体宽的表示介绍表明了系统的高特性。 | Albert W Cheng Haoyi Wang Hui Yang Linyu Shi Yarden Katz Thorold W Theunissen Sudharshan Rangarajan Chikdu S Shivalila Daniel B Dadon Rudolf Jaenisch | 2013 | Cell Research2013，23，10： | 65 |
| 2 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 3 | Digital spiral object identification using random light显示文摘Photons that are entangled or correlated in orbital angular momentum have been extensively used for remote sensing,object identification and imaging.It has recently been demonstrated that intensity fluctuations give rise to the formation of correlations in the orbital angular momentum components and angular positions of random light.Here we demonstrate that the spatial signatures and phase information of an object with rotational symmetries can be identified using classical orbital angular momentum correlations in random light.The Fourier components imprinted in the digital spiral spectrum of the object,as measured through intensity correlations,unveil its spatial and phase information.Sharing similarities with conventional compressive sensing protocols that exploit sparsity to reduce the number of measurements required to reconstruct a signal,our technique allows sensing of an object with fewer measurements than other schemes that use pixel-by-pixel imaging.One remarkable advantage of our technique is that it does not require the preparation of fragile quantum states of light and operates at both low-and high-light levels.In addition,our technique is robust against environmental noise,a fundamental feature of any realistic scheme for remote sensing. | Zhe Yang Omar S Magaña-Loaiza Mohammad Mirhosseini Yiyu Zhou Boshen Gao Lu Gao Seyed Mohammad Hashemi Rafsanjani Gui-Lu Long Robert W Boyd | 2017 | Light(Science & Applications)2017，6，1： | 2 |
| 4 | Role and relevance of polarity and hindrance of vinyl monomers in graft copolymerization onto potato starch显示文摘 | Fu Z S Liang W D Yang A M | 2002 | J Appl Polym Sci2002，85，4： | 2 |
| 5 | Mitochondrial Control Region Variability of Baiji and the Yangtze Finless Porpoises,two Sympatric Small Cetaceans in the Yangtze River显示文摘 | Yang G Liu S Ren W H | 2003 | Acta Theriologica2003，，48： | 1 |
| 6 | Bel-2 overexpresoion sensitizes MCF-7 cells to gcnintein by multiple mechanisms显示文摘 | Tophkhane C Yang S Bales W | 2007 | International Journal of Ontology2007，31，4： | 1 |
| 7 | Y-W Superplastie behavior in a two-phase TiAl alloy显示文摘 | Yang H S Kim | 1993 | Scripta Metallurgica et Materialia1993，，29： | 1 |
| 8 | Molecularly imprinted sol-gel nanotubes membrane for biochemical separations显示文摘 | Yang H H Zhang S Q Yang W | 2004 | Am Chem Soc2004，126，13： | 1 |
| 9 | Electrocatalytic properties of platinum on hard carbon spherules derived from deoiled asphalt for methanol oxidation显示文摘 | Xu B S Guo J J Jia H S Yang X W Liu X G | 2007 | Catalysis Today2007，125，34： | 1 |
| 10 | Extended vertical lower trapezius island myocutaneous flap for repairing extensive oropharyngeal defects显示文摘 | CHEN W L LI J S YANG Z H | 2009 | Oal Maxillofac Surg2009，67，： | 1 |
| 11 | Effect of surface composi-tion of Pt-Au alloy cathode catalyst on the performance of directmethanol fuel cells显示文摘 | XU J B ZHAO T S YANG W W | 2010 | International Journal of Hydrogen Energy2010，16，35： | 1 |
| 12 | An assessment of numerical parameters in- fluencing springback in explicit finite element analysis of sheet metal forming process 显示文摘 | Lee S W Yang D Y | 1998 | journal of materials processing tech- nolowj1998，，8081： | 1 |
| 13 | Silicon rutile from podiform chromite of the Imohusa ophiolite, Tibel显示文摘 | Yang J S Bai W J Fang Q S | 2003 | Progress in Natural Sciences2003，，23： | 1 |
| 14 | Minimizing the total machine workload for the wafer probing scheduling problem显示文摘 | Pearn W L Chung S H Yang M H | 2002 | IIE Transactions2002，34，2： | 1 |
| 15 | Storm wave reactivation of a submarine landslide显示文摘 | Prior D B Suhayda J N Lu N J Bomhold B D Keller G H Wiseman W J Wright L D Yang Z S | 1989 | Nature1989，341，： | 1 |
| 16 | Motion-compensated residue preprocessing in video coding based on just-noticeable-distortion profile显示文摘 | YANG X K LIN W S LU Z K | 2005 | IEEE Transactions on Circuits and Systems for Video Technology2005，15，6： | 1 |
| 17 | Allele-specific diferential expression of a common adiponectin gene polymorphism related to obesity显示文摘 | Yang W S Tsou P L Lee W J | 2003 | J Mol Med2003，81，： | 1 |
| 18 | Temperature-Dependent Photoluminescence of ZnO Nanorods Prepared by a Simple Solution Route显示文摘 | Lv Y Z Zhang Y H Li C P Ren L R Guo L Xu H B Ding L Yang C L Ge W K and Yang S H | 2007 | Luminescence2007，，： | 1 |
| 19 | The Cournot competition in the spatial equilibrium model显示文摘 | Yang C W Hwang M J Sohng S N | 2002 | Energy Economics2002，24，2： | 1 |
| 20 | Epidemiological serosurvey of hep-atitis 6 in China-declining HBV prevalence due to hepatitis Bvaccination 显示文摘 | Liang X Bi S Yang W | 2009 | Vaccine2009，27，47： | 1 |
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