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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | FOUR NEW GLYCOSIDES, LIGUROBUSTOSIDES A, B, C AND D FROM LIGUSTRUM ROBUSTUM显示文摘FOURNEWGLYCOSIDES,LIGUROBUSTOSIDESA,B,CANDDFROMLIGUSTRUMROBUSTUM￥JunTIAN;HongJieZHANGandHanDongSUN.(Laboratoryofphytochemistr... | Tian, J Zhang, HJ Sun, HD Pan, LT Yao, P Chen, DY | 1996 | Chinese Chemical Letters1996，7，4： | 3 |
| 3 | Phylogeo- graphic differentiation of mitochondrial DNA in Han Chinese显示文摘 | Yao YG Kong QP Bandelt HJ et ol | 2002 | Am J Hum Genet2002，70，3： | 1 |
| 4 | Phylogeographic differentiation of mitoehondrial DNA in Han Chinese 显示文摘 | Yao YG Kong OP Bandelt HJ | 2002 | Am J Hum Genet2002，70，3： | 1 |
| 5 | Effects of a biocontrol agent and methyljasmonate on postharvest disease of peach fruit and possiblemechanisms involved 显示文摘 | Yao HJ Tian SP | 2005 | Journal of Applied Microbiology2005，98，： | 1 |
| 6 | Effect of weathering treatment on the fractionation and leaching behavior of copper in municipal solid waste in- cinerator bottom ash 显示文摘 | Yao J Li WB Tang ML Fang CR Feng HJ Shen DS | 2010 | Chemosphere2010，81，： | 1 |
| 7 | Effect of heat flux on ablation behavior and mechanism of C/C-Zr B2-Si C composite under oxyacetylene torch flame显示文摘 | LI HJ YAO XY ZHANG YL | 2013 | Corrosion Science2013，74，： | 1 |
| 8 | Effects of all - trans retinoic acid on angiotensin Ⅱ- induced myocyte hypertrophy显示文摘 | Wang HJ Zhu Y C Yao T | 2002 | J Appl Physiol2002，92，5： | 1 |
| 9 | Downregulation of miR-199a may play a role in3-nitropropionic acid induced ischemic tolerance in rat brain显示文摘 | Xu WH Yao XY Yu HJ | 2012 | Brain Res2012，1429，： | 1 |
| 10 | Phylogeographic differentiation of mitochondrial DNA in Han Chinese显示文摘 | Yao YG Kong QP Bandelt HJ | 2002 | Am J Hum Genet2002，70，3： | 1 |
| 11 | Phylogeographic differentiation of mitochondrial DNA in Han Chinese显示文摘 | Yao YG Kong QP Bandelt HJ | 2002 | Am J Hum Genet2002，70，3： | 1 |
| 12 | Interaction between lung cancer cell and myofibroblast influenced by cyclic tensile strain显示文摘 | HuangJ W Pan HJ Yao W y | 2013 | Lab Chip2013，13，6： | 1 |
| 13 | integrin inhibition dramatical-ly enhances radiotherapy efficacy in human breast cancer xenografts显示文摘 | Park CC Zhang HJ Yao ES | 2008 | Cancer Res2008，68，11： | 1 |
| 14 | Effect of total flavonoids of epimedium on liver microsomal CYPIA2, CYP3A4 and CYP2E1 activities in rats显示文摘 | Hu DD Yao HJ Gu L | 2008 | J Chin Pharm Sci2008，17，2： | 1 |
| 15 | Downregulation of miR- 199a may play a role in 3-nitropropionic acid induced ische- mic tolerance in rat brain 显示文摘 | Xu WH Yao XY Yu HJ | 2012 | Brain Res2012，1429，： | 1 |
| 16 | β1 integrin inhibition dramatically enhances radiotherapy efficacy in human breast cancer xenografts显示文摘 | Park CC Zhang HJ Yao ES | | 0，，11： | 1 |
| 17 | Simultaneous determination of four major constituents of Semen Vaecariae Using HPLC- DAD显示文摘 | Zhang HJ Yao W Chen YY | 2012 | Natural Product Communications2012，9，7： | 1 |
| 18 | A combined approach of physicochemical and biological methods for the characterization of petroleum hydrocarbon-contaminated soil 显示文摘 | Masakorala K Yao J Chandankere R Liu HJ Liu WJ Cai MM Choi MMF | 2014 | Environ Sci Pollut R2014，21，： | 1 |
| 19 | Five New Glycosides, Ligurobustosides E, F, I, J and K from Ligustrum Robustum显示文摘- Five new monoterpenoid glycosides named ligurobustosides E (1), F (2), I (3), J (4) and K (5) were isolated from the leaves of Ligustrum robustum. Their structures were established by spectroscopic and chemical methods. | Tian, J Zhang, HJ Sun, HD Pan, LT Yao, P Chen, DY | 1997 | Chinese Chemical Letters1997，8，2： | 1 |
| 20 | Short-term effect of aniline on soil microbial activity: a combined study by isothermal microcalorimetry,glucose analysis,and enzyme assay techniques 显示文摘 | Chen HL Zhuang RS Yao J Wang F Qian YG Masakorala K Cai MM Liu HJ | 2014 | Environ Sci Pollut R2014，21，： | 1 |
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