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| 1 | Inuence of oxytetracycline on the structure and activity of microbial community in wheat rhizosphere soil显示文摘The microbial community composition in wheat rhizosphere was analyzed by detecting colony forming units (CFUs) in agar plates. The total CFUs in rhizosphere were 1.04 × 109/g soil with 9.0 × 108/g bacteria, 1.37 × 108/g actinomyces and 3.6 × 106/g fungi. The 10 dominant bacteria were isolated from wheat rhizosphere and were grouped into genus Bacillus according to their full length 16S rRNA gene sequences. Although belonging to the same genus, the isolated strains exhibited dierent sensitivities to oxytetracycline. When a series of the rhizosphere soil was exposed under various concentrations of oxytetracycline, the microbial community structure was highly aected with significant decline of CFUs of bacteria and actinomyces (22.2% and 31.7% at 10 mg/kg antibiotic, respectively). This inhibition was clearly enhanced with the increase exposure dosage of antibiotic and could not be eliminated during 30 d incubation. There was no obvious inuence of this treatment on fungi population. Among the four soil enzymes (alkaline phosphatase, acidic phosphatase, dehydrogenase and urease), only alkaline phosphatase was sensitive to oxytetracycline exposure with 41.3% decline of the enzyme activity at 10 mg/kg antibiotic and further decrease of 64.3%-80.8% when the dosage over 30 mg/kg. | YANG Qingxiang, ZHANG Jing, ZHU Kongfang, ZHANG Hao College of Life Sciences, Henan Normal University, Xinxiang 453007, China. | 2009 | Journal of Environmental Sciences2009，21，7： | 36 |
| 2 | Targeting Gene-Virotherapy of Cancer and its prosperity显示文摘为癌症的基因和病毒的治疗显示出一些治疗学的效果,但是有真实突破的缺乏。为了达到肿瘤的完全的消除的目标,在动物的异种皮移植当模特儿,我们开发了叫的新策略指向癌症的 Gene-Virotherapy,它试图联合基因治疗和 virotherapy 的优点。这新策略独自比基因或病毒的治疗生产了更强壮的反肿瘤效果。肿瘤特定的 replicative 侵入人体气管粘膜的病菌向量,指定了为 ZD55,被侵入人体气管粘膜的病菌的 55kDa E1B 区域的删除构造。结果病毒的构造不仅由明确地指向 p53 保留类似的功能到 ONYX-015 否定肿瘤,而且允许各种各样的治疗学的基因到的插入由于最新介绍的克隆的地点形成适当 ZD55 衍生物,与原来的 ONYX-015 病毒不可行的一项任务。我们证明反肿瘤一如此的衍生物完成, ZD55-IL-24,比 ZD55 virotherapy 或 Ad-IL-24 基因治疗的是乘更多的有势力的至少 100。不过,由 ZD55-IL-24 的使用的肿瘤质量的完全的消除是仅仅在一些然而并非所有老鼠观察了,显示那一治疗学的基因不对“痊愈”足够这些老鼠。有互补或合作的效果的基因是什么时候,独立克隆进 ZD55 向量并且在联合使用了(指定了为双基因治疗策略) ,好一些的结果被获得;并且如果二合适的基因被选择,在所有老鼠完成所有异种皮移植肿瘤群众的完全的消除是可能的。更全面的研究将多半为临床的试用基于这新策略导致一个协议。最后,为癌症治疗的两倍控制指向的病毒双的基因治疗的概念,和在癌症干细胞的最近的进步的含意也被讨论。 | Xin Yuan Liu~(1,2) ~1Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,320 Yue Yang Road,Shanghai 200031,China ~2Xinyuan Institute of Medicine and Biotechnology,School of Life Science,Zhejiang Sci-Tech University,Hangzhou 310018,China | 2006 | Cell Research2006，16，11： | 32 |
| 3 | Neuroprotection of Glycyrrhizin against Ischemic Vascular Dementia in Vivo and Glutamate-induced Damage in Vitro显示文摘Objective To evaluate the neuroprotective effects of glycyrrhizin(GL) on vascular dementia(VaD) in vivo and Glutamate-induced damage in vitro.Methods Male Wistar rats were subjected to permanent occlusion of the bilateral common carotid arteries.On d 15 postsurgery,rat cognition was assessed using the Morris water maze.The activity of superoxide dismutase and the content of malondialdehyde of brains were also measured.Brain damage was evaluated histologically using HE staining.In vitro cell viability was examined in PC12 cells exposed to Glutamate,and mRNA levels of Bcl-2 and Bax were assessed.Results GL(20 mg/kg for 12 d) improved the performance of learning and memory of VaD rats,decreased the level of lipid peroxidation,and attenuated the pathological alterations in the hippocampal CA1 and CA3 areas.Moreover,GL(0.6 mmol/L) could protect PC12 cell lines from injury induced by Glutamate(10 mmol/L) and inhibit apoptosis of neuronal cells.Conclusion The present findings suggest that GL may have therapeutic potential in treating VaD. | HOU Yuan-yuan1,YANG Yang1,2,YAO Yang2,BAI Gang1,2 1.College of Pharmacy,Nankai University,Tianjin 300071,China 2.College of Life Sciences,Nankai University,Tianjin 300071,China | 2010 | Chinese Herbal Medicines2010，2，2： | 24 |
| 4 | Construction, expression and characterization of the engineered antibody against tumor surface antigen, p185^(c-erbB-2)显示文摘The c-erbB-2 proto-oncogene encodes a 185kDa protein p185, which belongs to epidermal growth factorreceptor family. Amplification of this gene has been shown to correlate with poor clinical prognosis forcertain cancer patients. The monoclonal antibody A21 which directed against p185 specifically inhibitsproliferation of tumor cells overexpressing p185, hence allows it to be a candidate for targeted therapy. Inorder to overcome several drawbacks of murine MAb, we cloned its VH and VL genes and constructed thesingle-chain Fv (scFv) through a peptide linker. The recombinant scFvA21 was expressed in Escherichiacoli and purified by the affinity column. Subsequently it was characterized by ELISA, Western blot, cellimmunohistochemistry and FACS. All these assays showed the binding activity to extracellular domain(ECD) of p185. Based on those properties of scFvA21, we further constructed the scFv-Fc fusion moleculewith a homodimer form and the recombinant product was expressed in mammalian cells. In a series ofsubsequent analysis this fusion protein showed identical antigen binding site and activity with the parentantibody. These anti-p185 engineered antibodies have promised to be further modified as a tumor targetingdrugs, with a view of application in the diagnosis and treatment of human breast cancer. | LIAN SHENG CHENG, AI PING LIU, JIA HONG YANG, YAN QIU DONG, LIANG WEI LI, JING WANG, CHAO CHEN WANG, JING LIUSchool of Life Science, University of Science and Technology of China, Hefei 230027, China | 2003 | Cell Research2003，13，1： | 24 |
| 5 | Effects of silica nanoparticles on growth and photosynthetic pigment contents of Scenedesmus obliquus显示文摘To assess the aquatic ecosystem safety for silica (SiO2) nanoparticles (NPs), the growth inhibition and photosynthetic pigment contents of Scenedesmus obliquus in logarithm growth phase exposed to SiO2 NPs and SiO2 bulk particles (BPs) suspensions were measured. SiO2 NPs with 10-20 nm diameters were found to be toxic. The 20% effective concentration (EC20) values for 72 and 96 hr were 388.1 and 216.5 mg/L, respectively. The contents of chlorophyll decreased significantly under moderate and high concentration (50, 100, and 200 mg/L) of SiO2 NPs after 96-hr exposure, but the carotenoids did not. SiO2 BPs were found to be nontoxic up to 200 mg/L. The toxicity of SiO2 NPs probablely due to their sorption to algal cells surface. The results imply that there is potential harm to aquatic environment by using SiO2 NPs, and it should deserve special concern. | Chenxi Wei, Yanbo Zhang, Jing Guo, Bing Han, Xu Yang, Junlin Yuan Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, China. | 2010 | Journal of Environmental Sciences2010，22，1： | 14 |
| 6 | AtMYB103 is a crucial regulator of several pathways affecting Arabidopsis anther development显示文摘Previous reports indicated that AtMYB103 has an important role in tapetum development,callose dissolution,and exine formation in A.thaliana anthers.Here,we further characterized its function in anther development by expression pattern analysis,transmission electron microscopy observation of the knockout mutant,and microarray analysis of downstream genes.A total of 818 genes differentially expressed between ms188 and the wild-type were identified by global expression profiling analysis.Functional classification showed that loss-of-function of AtMYB103 impairs cell wall modification,lipid metabolic pathways,and signal transduction throughout anther development.RNA in situ hybridization confirmed that transcription factors acting downstream of AtMYB103 (At1g06280 and At1g02040) were expressed in the tapetum and microspores at later stages,suggesting that they might have important roles in microsporogenesis.These results indicated that AtMYB103 is a crucial regulator of Arabidopsis anther development. | ZHU Jun1,ZHANG GuoQiang2,CHANG YuHua2,LI XiaoChuan2,YANG Jun2,HUANG XueYong2,YU QingBo2,CHEN Hui3,WU TianLong1* & YANG ZhongNan2* 1College of Agriculture and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China 2College of Life and Environmental Sciences,Shanghai Normal University,Shanghai 200234,China 3Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China | 2010 | Science China(Life Sciences)2010，53，9： | 10 |
| 7 | Genetic and physical fine- mapping of the Sc locus conferring indica-japonica hybrid sterility in rice (Oryza sativa L.)显示文摘Hybrid sterility is a major hindrance to utiliz-ing the heterosis in indica-japonica hybrids. To isolate a gene Sc conferring the hybrid sterility, the locus was mapped us-ing molecular markers and an F2 population derived from a cross between near isogenic lines. A primary linkage analysis showed that Sc was linked closely with 4 markers on chro-mosome 3, on which the genetic distance between a marker RG227 and Sc was 0.07 cM. Chromosome walking with a rice TAC genomic library was carried out using RG227 as a starting probe, and a contig of ca. 320 kb covering the Sc locus was constructed. Two TAC clones, M45E14 and M90J01 that might cover the Sc locus, were partially se-quenced. By searching the rice sequence databases with se-quences of the TACs and RG227 a japonica rice BAC se-quence, OSJNBb0078P24 was identified. By comparing the TAC and BAC sequences, six new PCR-based markers were developed. With these markers the Sc locus was further mapped to a region of 46 kb. The results suggest that the BAC OSJNBb0078P24 and TAC M45E14 contain the Sc gene. Six ORFs were predicted in the focused 46-kb region. | YANG Cunyi1,2 *, CHEN Zhongzheng1*, ZHUANG Chuxiong1, MEI Mantong1 & LIU Yaoguang1 1. College of Life Science, South China Agricultural University, Guang-zhou 510642, China 2. Root Biology Center, South China Agricultural University, Guangzhou 510642, China | 2004 | Chinese Science Bulletin2004，49，16： | 8 |
| 8 | Promoter polymorphism of MRP1 associated with reduced survival in hepatocellular carcinoma显示文摘AIM:To investigate the effect of the G-1666A polymorphism in the multidrug resistance related protein-1 (MRP1) on outcome of hepatocellular carcinoma (HCC). METHODS:A cohort of 162 patients with surgically resected HCC who received no postsurgical treatment until relapse was studied. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism analysis. Electrophoretic mobility shift assay (EMSA) was used to evaluate the influence of the G-1666A polymorphism on the binding affinity of the MRP1 promoter with its putative transcription factors. RESULTS:Kaplan-Meier analysis showed that patients with GG homologues had a reduced 4-year disease-free survival compared with those carrying at least one A allele (P = 0.011). Multivariate Cox regression analysis indicated that the-1666GG genotype represented an independent predictor of poorer disease-free survival [hazard ratio (HR) = 3.067,95% confidence interval (CI):1.587-5.952,P = 0.001],and this trend became worse in men (HR = 3.154,95% CI:1.604-6.201,P = 0.001). A similar association was also observed between 4-year overall survival and the polymorphism in men (HR = 3.342,95% CI:1.474-7.576,P = 0.004). Moreover,EMSA suggested that the G allele had a stronger binding affinity to nuclear proteins. CONCLUSION:The MRP1-1666GG genotype predicted a worse outcome and was an independent predictor of poor survival in patients with HCC from Southeast China. | Jing Zhao,Bing-Yun Yu,Jin-E Yang,Key Laboratory of Gene Engineering of the Ministry of Education,School of Life Sciences,Sun Yat-sen (Zhongshan) University,Guangzhou 510275,Guangdong Province,China Dao-Yuan Wang,Department of Medical Oncology,The First Affiliated Hospital of Guangzhou Medical College,Guangzhou 510120,Guangdong Province,China | 2010 | World Journal of Gastroenterology2010，16，48： | 8 |
| 9 | The molecular characterization of maize B chromosome specific AFLPs显示文摘The origin and evolution of B chromosomes could be explained by the specific DNA sequence on them. But the specific sequences known were quite limited. To investigate maize B chromosome sqicific DNA sequeces, maize genomes with and without B chromosomes were analyzed by AFLP. Only 5 markers were found specific to genomes with B chromosomes among about 2000 AFLP markers. Southern hybridization and sequence analysis revealed that only the sequence of M8-2D was a B chromosome specific sequence. This sequence contained the telomeric repeat unit AGGGTTT conserved in plant chromosome telomeres. In addition, the sequence of M8-2D shared low homology to clones from maize chromosome 4 centromere as well. M8-2D were localized to B chromosome centrorneric and telomeric regions. | ZHONG XIA QI, Hui ZENG, Xiu LAN LI, CHENG BIN CHEN, WEN QIN SONG, RUI YANG CHEN The College of Life Sciences, Nankai University, Tianjin 300071, China | 2002 | Cell Research2002，12，1： | 7 |
| 10 | Maize ZmFDR3 localized in chloroplasts is involved in iron transport显示文摘Iron is an essential nutrient for plant metabolism such that Fe-limited plants display chlorosis and suffer from reduced photosynthetic efficiency. Differential display previously identified genes whose expression was elevated in Fe-deficient maize roots. Here,we describe the functional characterization of one of the genes identified in the screen,ZmFDR3 (Zea maize Fe-deficiency-related). Heterologous functional complementation assays using a yeast iron uptake mutant showed that ZmFDR3 functions in iron transport. ZmFDR3 contains a domain found in FliN-proteins of the type III secretion system and is predicted to localize to the thylakoid of plastids. Fluorescence immunocytochemistry showed that ZmFDR3 is localized in the plastids of roots,stems and leaves,with high expression found in guard cell chloroplasts. Transgenic tobacco expressing a 35S-ZmFDR3 construct contains elevated iron content,displays well arranged thylakoid membranes and has photosynthetic indices that are higher than those of the wild type. Together,these results suggest that ZmFDR3 functions in chloroplast iron transport. | HAN JianHui1,SONG XiuFang1,LI Peng1,YANG HuiJun2 & YIN LiPing1 1 College of Life Science,Capital Normal University,Beijing 100048,China 2 Department of Biological Sciences,University of South Carolina,Columbia,SC 29208,USA | 2009 | Science China(Life Sciences)2009，52，9： | 7 |
| 11 | A Preliminary Study of Crassulacean Acid Metabolism (CAM) in the Endangered Aquatic Quillwort Isoetes sinensis Palmer in China显示文摘Isoetes sinensis Palmer (Isoetaceae) is an aquatic or amphibious plant that is critically endangered in China. Previous studies have revealed the crassulacean acid metabolism (CAM)-like photosynthetic pathway occurs com-monly in submerged leaves in genus Isoetes. Water chemistry parameters and the titratable acidity content of the plant extract were measured from samples obtained in the early morning (7:00) and late afternoon (15:00) from two I.sinensis populations in China. One population occurs in the eulittoral zone of a freshwater tidal river at low elevation (134 m) and another occurs in a densely vegetated, high elevation (1 100 m) alpine shallow pool. Significant differences in pH and titratable acidity of the plant extract were detected between the morning and afternoon samples. These changes are associated with diurnalchanges in water chemistry. Our results provide the first evidence for the exist-ence of the CAM pathwa in the East Asian endemic Isoetes sinensis Palmer.The magnitude of fluctuations in the titratable acidity of the plant extract mayb e correlated with the severe carbon limitation imposed on the plants by its aquatic habitat. | Pang Xin-an, Wang Qing-feng, Gituru W. Robert, Liu Hong, Yang Xiao-lin, Liu XingCollege of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China | 2003 | Wuhan University Journal of Natural Sciences2003，8，02A： | 6 |
| 12 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 13 | Structural and functional analysis of denitrification genes in Pseudomonas stutzeri A1501显示文摘Four gene clusters associated with denitrification were identified in the genome of A1501 strain, nar, nir, nor and nos, including 40 genes totally, which encode proteins for sub-stance transportation, gene regulation and reductases. The three gene clusters, nir, nor and nos are adjacent on chromosome and are far from nar gene cluster. Compared with other denitrifying bacteria, the 40 denitrification genes in A1501 strain compose a complete denitrification catalysis system. In A1501 strain, this system has the following characteristics: (i) only one copy of narK gene is found in nar gene cluster; (ii) a narM gene is present between narK and narG; (iii) two genes, dnarE and orfl are identified at downstream of narX and narL genes, of which dnrE per-haps is a transcriptional factor belonging to FNR family; (iv) there are 16 nir genes in A1501, the most in the known denitrifying bacteria; (v) it is for the first time that norR gene has been found in A1501 and also in Pseudomonas; (vi) nos gene cluster is relatively conservative, with a com-pletely identical composition and arrangement of genome to the reference bacteria strain. | YAN Yongliang1,2, YANG Jian2, CHEN Lihong2, YANG Fan2, DONG Jie2, XUE Ying2, XU Xingye2, ZHU Yafang2, YAO Zhijian3, LIN Min4, WANG Yiping5 & JIN Qi1,2 1. College of Biological Sciences, China Agricultural University, Beijing 100094, China 2. State Key Laboratory for Moleclular Virology and Genetic Engineering, Beijing 100176, China 3. National Center for Human Genome Research, Beijing 100176, China 4. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China 5. College of Life Sciences, Peking University, Beijing 100871, China | 2005 | Science China(Life Sciences)2005，48，6： | 6 |
| 14 | Complex positive selection pressures drive the evolution of HIV-1 with different co-receptor tropisms显示文摘HIV-1 co-receptor tropism is central for understanding the transmission and pathogenesis of HIV-1 infection. We performed a genome-wide comparison between the adaptive evolution of R5 and X4 variants from HIV-1 subtypes B and C. The results showed that R5 and X4 variants experienced differential evolutionary patterns and different HIV-1 genes encountered various positive selection pressures, suggesting that complex selection pressures are driving HIV-1 evolution. Compared with other hypervariable regions of Gp120, significantly more positively selected sites were detected in the V3 region of subtype B X4 variants, V2 region of subtype B R5 variants, and V1 and V4 regions of subtype C X4 variants, indicating an association of positive selection with co-receptor recognition/binding. Intriguingly, a significantly higher proportion (33.3% and 55.6%, P<0.05) of positively selected sites were identified in the C3 region than other conserved regions of Gp120 in all the analyzed HIV-1 variants, indicating that the C3 region might be more important to HIV-1 adaptation than previously thought. Approximately half of the positively selected sites identified in the env gene were identical between R5 and X4 variants. There were three common positively selected sites (96, 113 and 281) identified in Gp41 of all X4 and R5 variants from subtypes B and C. These sites might not only suggest a functional importance in viral survival and adaptation, but also imply a potential cross-immunogenicity between HIV-1 R5 and X4 variants, which has important implications for AIDS vaccine development. | ZHANG ChiYu1*, DING Na1, CHEN KePing1 & YANG RongGe2* 1Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China 2HIV Molecular Epidemiology and Virology Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China | 2010 | Science China(Life Sciences)2010，53，10： | 5 |
| 15 | The role of the DLPFC in inductive reasoning of MCI patients and normal agings:An fMRI study显示文摘Previous studies of young people have revealed that the left dorsolateral prefrontal cortex(DLPFC) plays an important role in inductive reasoning.An fMRI experiment was performed in this study to examine whether the left DLPFC was involved in inductive reasoning of MCI patients and normal agings,and whether the activation pattern of this region was different between MCI patients and normal agings.The fMRI results indicated that MCI patients had no difference from normal agings in behavior performance(reaction time and accuracy) and the activation pattern of DLPFC.However,the BOLD response of the DLPFC region for MCI patients was weaker than that for normal agings,and the functional connectivity between the bilateral DLPFC regions for MCI patients was significantly higher than for normal agings.Taken together,these results indicated that DLPFC plays an important role in inductive reasoning of agings,and the functional abnormity of DLPFC may be an earlier marker of MCI before structural alterations. | YANG YanHui1,3,LIANG PeiPeng2,3,LU ShengFu2,3,LI KunCheng1,3 & ZHONG Ning2,3,4 1 Xuanwu Hospital,Capital Medical University,Beijing 100053,China 2 The International WIC Institute,Beijing University of Technology,Beijing 100124,China 3 Beijing Municipal Lab of Brain Informatics,Beijing 100124,China 4 Department of Life Science and Informatics,Maebashi Institute of Technology,Maebashi 371-0816,Japan | 2009 | Science China(Life Sciences)2009，52，8： | 5 |
| 16 | Analysis of expressed sequence tags of a marine red alga,Gracilaria lemaneiformis显示文摘The expressed sequence tags (EST) has been proved to be a useful tool for discovering and identifying functional genes, especially in some species whose genetic information is unavailable. A total of 180 ESTs have been generated from a cDNA library of gametophytic Gracilaria lemaneiformis in this study. These clones are clustered into 151 groups, among which 8 groups are highly homologous to chloroplast genes and are abundant in the library. After searching for matches in the EST database of red alga, 22 groups are found to match with the registered ESTs of Rhodophyta and 6 with Gracilaria. Searching in the protein database reveal that 73 non-redundant clones have significant similarity to some known sequences, the majority of which are involved in photosynthesis, DNA transcription or translation, and 6, 4 and 3 clones are associated with growth or development, signal transduction and stress or defense response, respectively. | SUN Xue, YANG Guanpin, MAO Yunxiang, ZHANG Xuecheng, SUI Zhenghong and QIN Song(1. College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003, China 2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China) | 2002 | Progress in Natural Science:Materials International2002，12，7： | 4 |
| 17 | Template synthesis of N——F-codoped TiO_2 nanotubes with high visible light activity显示文摘A series of highly ordered N-F-codoped TiO2 nanotubes were synthesized with a simple template technique and the obtained samples were detected by FE-SEM and XPS. The UV-Vis absorption spectrum showed that after N-F-codoping,TiO2 nanotubes had a new absorption band at 425 nm,leading to a photoresponse in the visible region. These modified nanotubes showed a significant visibleinduced photocatalytic activity for the degradation of methylene blue in aqueous solution. | PAN SuJuan1,LIANG HaiChao2,WANG ChangQing1,YANG FuWei1,LI XiaoDong1 & YANG Sheng1 1 Department of Life Science and Chemistry,Tianshui Normal University,Tianshui 741000,China 2 Department of Civil and Structural Engineering,The Hong Kong Polytechnic University,Hong Kong,China | 2009 | Science China Chemistry2009，52，12： | 4 |
| 18 | Epstein-Barr virus-encoded latent membrane protein 1 modulates cyclin D1 by c-Jun/Jun B heterodimers显示文摘In our recent studies, we found that LMP1 encoded by Epstein-Barr virus could accelerate the formation of active c-Jun/Jun B heterodimer. We studied the regulation of cyclinD1 by c-Jun/Jun B heterodimers by laser scanning confocal influorescence microscopy, Western blot, luciferase activity assay, super-EMSA and flow cytometry in the Tet-on-LMP1 HNE2 cell line, in which LMP1 expression was regulated by Tet-on system. c-Jun/Jun B heterodimers induced by LMP1 could up regulate cyclin D1 promoter activity and expression. Overexpression of cy-clinD1 accelerated the progression of cell cycle. | SONG Xin1, TAO Yongguang1, ZENG Liang1, YANG Jing1, TANG Faqing1, Leo M. Lee2, GONG Jianping3, WU Qiao4 & CAO Ya1 1. Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China 2. Laboratory of Molecular Technology SAIC-Frederick, National Cancer Institute P.O. Box B, Frederick, MD 21702, USA 3. Molecular Medical Center, Tongji Hospital, Tongji Medical University, Wuhan 430030, China 4. Key Laboratory of the Ministry of Education for Cell Biology, and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China | 2005 | Science China(Life Sciences)2005，48，4： | 4 |
| 19 | Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter显示文摘As the main structural protein of oil body,OLEOSIN is highly expressed only during seed development. OLEOSIN promoter is a very useful tool for seed-specific gene engineering and seed bioreactor designing. The B3 domain transcription factor leafy cotyledon2 (LEC2) plays an important role in regulating seed development and seed-specific gene expression. Here,we first report how seed-specific B3 domain transcription factor leafy cotyledon2 (LEC2) efficiently activates OLEOSIN expression. The central promoter region of OLEOSIN,responsible for seed specificity and LEC2 activation,was determined by 5'-deletion analysis. Binding experiments in yeast cells and electrophoretic mobility shift assays showed that LEC2 specifically bound to two conserved RY elements in this region. In transient expression assays,mutation in either RY element dramatically reduced LEC2 activation of OLEOSIN promoter activity,while double mutation abolished it. Analysis of the distribution of RY elements in seed-specific genes activated by LEC2 also supported the idea that genes containing neighboring RY elements responded strongly to LEC2 activation. Therefore,we conclude that two neighboring RY elements are essential for efficient LEC2 activation of OLEOSIN expression. These findings will help us better utilize seed-specific promoter activity. | CHE NanYing,YANG Yang,LI YanDong,WANG LiLi,HUANG Ping,GAO Yin & An ChengCai The National Laboratory of Protein Engineering and Plant Genetic Engineering,School of Life Sciences,Peking University,Beijing 100871,China | 2009 | Science China(Life Sciences)2009，52，9： | 4 |
| 20 | Immunohistochemical localization of Ca^(2+)/calmodulin-dependent kinase in tobacco显示文摘The existence of Ca2+/calmodulin-dependent kinase (CaM kinase, CaMK) in tobacco is verified immuno-logically and its distribution in different tissues of tobacco is studied. It has been demonstrated that CaMK is mainly distributed in early developing anthers, developing ovules and embryos, lateral root primordium, apical meristem and leaf primordium of buds and mesophyll cells and developing vascular bundles of leaves. There is enormous CaM kinase distributed in leaf epidermis fair cells and guard cells of stomas too. Little kinase is found in mature stem or root cells. The distribution properties of CaM kinase in tobacco are consistent with those of CaM, suggesting that there exists the Ca2+ signal transduction pathway mediated by CaM kinase in tobacco and it plays an important role in the plant growth and development. | YANG Wannian, LIANG Shuping & LU YingtangCenter of Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China | 2001 | Chinese Science Bulletin2001，46，3： | 4 |
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