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| 1 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 2 | Effects of Heat Treatment on the Transformation Temperature and the Microstructure of Ni-Ti-Nb Shape Memory Alloy显示文摘The effects of heat treatment on the phase transformation temperature and the microstructure of Ni-Ti-Nb alloy have been investigated by means of R-T method, TEM and EPMA. With the increase of annealing temperature, cooling rate and ageing temperature, the Ms temperature increases. TEM observations show no new precipitated phase was found in the B2 parent phase after heat treatment, but EPMA shows the content of Ni (or Ni/Ti ratio) in the B2 matrix changed. The main reason for the change of transformation temperature lies in the change of the matrix composition (or Ni/Ti ratio) with the variation of heat treatment procedure. | Zheng, YF Cai, W Wang, YQ Luo, YC Zhao, LC | 1998 | Journal of Materials Science & Technology1998，14，1： | 4 |
| 3 | Apigenin induced apoptosis through p53-dependent pathway in human cervical carcinoma cells显示文摘 | Zheng PW Chiang LC Lin CC | 2005 | Life Sci2005，76，12： | 1 |
| 4 | Apigenin induced apoptosis through p53-dependent pathway in human cervical carci-noma cells显示文摘 | Zheng PW Chiang LC Lin CC | 2005 | Life Sci2005，76，12： | 1 |
| 5 | Fnl4-Fe Fusion protein regulates atherosclerosis in ApoE -/- mice and inhibits macro- phage lipid uptake in vitro 显示文摘 | Schapira K Burkly LC Zheng TS | 2009 | Arterioscler Thromb Vasc Biol2009，29，12： | 1 |
| 6 | Optimal cooperative jamming to enhance physical layer security using relays显示文摘 | Zheng G Choo LC | 2011 | IEEE Trans SP2011，59，3： | 1 |
| 7 | Apigenin induced apoptosis through p53-dependent pathway in human cervical carcinoma cells显示文摘 | Zheng PW Chiang LC Lin CC | 2005 | Life Sci2005，76，12： | 1 |
| 8 | TWEAK/Fnl4 pathway: an immuno logical switch for shaping tissue responses 显示文摘 | Burkly LC Michaelson JS Zheng TS | 2011 | ImmunolRev2011，244，： | 1 |
| 9 | Apigenin induced apoptosis through p53-dependent pathway in human cervical carcinoma cells显示文摘 | ZHENG PW CHIANG LC LIN CC | 2005 | Life Sei2005，76，12： | 1 |
| 10 | A new method for modulating traumatic brain injury with mechanical tissue resuscitation 显示文摘 | Argenta LC Zheng Z Bryant A | 2012 | Neurosurgery2012，70，5： | 1 |
| 11 | Fnl4-Fc fusion protein regulates atherosclerosis in ApoE-/- mice and inhibits macrophage lipid uptake in vitro显示文摘 | Schapira K Burkly LC Zheng TS | 2009 | Arterioscler Thromb Vasc Biol2009，29，： | 1 |
| 12 | Notch-4 contributes to the metastasis ot salivary adenoid cystic carcinoma 显示文摘 | Ding LC She L Zheng DL | 2010 | Oncol Rep2010，24，2： | 1 |
| 13 | Notch-4 contributes to the metastasis of salivary adenoid cystic carcinoma显示文摘 | Ding LC She L Zheng DL | 2010 | Oncol Rep2010，24，： | 1 |
| 14 | Apigenin indueed apoptosis through p53-dependent pathway in human eervieal carcinoma cells 显示文摘 | Zheng PW Chiang LC Lin CC | 2005 | Life Sei2005，76，12： | 1 |
| 15 | Regulation of epithelial tight junction assemblyand disassembly by AMP-activated protein kinase显示文摘 | Zheng B Cantley LC | 2007 | Proc Natl AcadSci U S A2007，104，3： | 1 |
| 16 | Apigenin induced apoptosis through p53 -dependent pathway in human cervical carcinoma cells显示文摘 | Zheng PW Chiang LC Lin CC | 2005 | Life Sci2005，76，12： | 1 |
| 17 | A role for Fli-1 in B cellproliferation:implicationsfor SLE pathogenesis显示文摘 | Bradshaw S Zheng WJ Tsoi LC | 2008 | Clin Immunol2008，129，1： | 1 |
| 18 | Apigenin induced apoptosis through p53-dependent pathway in human hepatoma cells显示文摘 | Zheng PW Chiang LC Lin CC | 2005 | Life Sciences2005，76，12： | 1 |
| 19 | Notch-4 contributes to the metastasis of salivary adenoid cystic carcinoma显示文摘 | Ding LC She L Zheng DL | 2010 | Oncol Rep2010，24，2： | 1 |
| 20 | Fn14-Fc fusion protein regulates atherosclerosis in ApoE-/-mice and inhibits macrophage lipid uptake in vitro显示文摘 | Schapira K Burkly LC Zheng TS | | Arteriosclerosis0，，12： | 1 |
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