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| 1 | Nucleoporin 88 expression in hepatitis B and C virus-related liver diseases显示文摘AIM: To investigate the expression of nucleoporin 88 (Nup88) in hepatitis B virus (HBV) and C virus (HCV)-related liver diseases. METHODS: We generated a new monoclonal Nup88 antibody to investigate the Nup88 protein expression by immunohistochemistry (IHC) in 294 paraffin-embedded liver specimens comprising all stages of hepatocellular carcinogenesis. In addition, in cell culture experiments HBV-positive (HepG2.2.15 and HB611) and HBV-negative (HepG2) hepatoma cell lines were tested for the Nup88 expression by Western-immunoblotting to test data obtained by IHC.RESULTS: Specific Nup88 expression was found in chronic HCV hepatitis and unspecific chronic hepatitis, whereas no or very weak Nup88 expression was detected in normal liver. The Nup88 expression was markedly reduced or missing in mild chronic HBV infection and inversely correlated with HBcAg expression. Irrespective of the HBV- or HCV-status, increasing Nup88 expression was observed in cirrhosis and dysplastic nodules, and Nup88 was highly expressed in hepatocellular carcinomas. The intensity of Nup88 expression significantly increased during carcinogenesis (P < 0.0001) and correlated with dedifferentiation (P < 0.0001). Interestingly, Nup88 protein expression was significantly downregulated in HBV-positive HepG2.2.15 (P < 0.002) and HB611 (P < 0.001) cell lines as compared to HBV-negative HepG2 cells. CONCLUSION: Based on our immunohistochemical data, HBV and HCV are unlikely to influence the expression of Nup88 in cirrhotic and neoplastic liver tissue, but point to an interaction of HBV with the nuclear pore in chronic hepatitis. The expression of Nup88 in nonneoplastic liver tissue might reflect enhanced metabolic activity of the liver tissue. Our data strongly indicate a dichotomous role for Nup88 in non-neoplastic and neoplastic conditions of the liver. | Martina Knoess Anna Kordelia Kurz Olga Goreva Nuran Bektas Kai Breuhahn Magarethe Odenthal Peter Schirmacher Hans Peter Dienes C Thomas Bock Hanswalter Zentgraf Axel zur Hausen | 2006 | World Journal of Gastroenterology2006，12，36： | 4 |
| 2 | Comparison of two methods of adding jitter to artificial neural network training显示文摘 | JIANG Y METZ C E | 2004 | International Congress Series2004，1268，： | 1 |
| 3 | Novel M(II)-Hg(II) coordination polymers generated from metal-containing building blocks M (2-pyrazineearboxyliate)2 '(H2O)2 (M=Cu,Ni,Co)and HgCl2显示文摘 | Dong Y B Smith M D zur Loye H C | 2000 | Solid State Sci2000，，2： | 1 |
| 4 | Mag- netic resonance imaging contrast agent targeted toward activated platelets allows in vivo detection of thrombosis and monitoring of thrornbolysis显示文摘 | yon Zur Muhlen C yon Elverfeldt D Moeller J A | 2008 | Circulation2008，118，3： | 1 |
| 5 | A new mixed-metal AgCocoordinationpolymerassembledfromcobalt-containing molecular building blocks and AgNO3显示文摘 | Ciurtin D M Smith M D zur Loye H C | 2002 | Solid State Sci2002，，4： | 1 |
| 6 | Structural diversity in the Cu(pyrazineearboxylate)2/CdCl2 system:new one-, two-and three-dimensional mixed metal coordination polymers显示文摘 | Ciurtin D M Smith M D zur Loye H C | 2003 | Dalton2003，，： | 1 |
| 7 | Transoral trans-stomal microdebrider excision of tracheal papillomatosis显示文摘 | THORNE M C ZUR K B | 2009 | Laryngoscope2009，119，： | 1 |
| 8 | Solid lipid nanoparticles (SLN)for controlled drug delivery-Drug release and release mechanism显示文摘 | zur Mühlen A Schwarz C Mehnert W | 1998 | Eur J Pharm B iopharm1998，45，2： | 1 |
| 9 | 显示文摘 | Tilford R W Gemmill W R zur Loye H C | 2006 | Chemistry of materials2006，18，22： | 1 |
| 10 | In- creased apoptosis in gastric mucosa adjacent to intestinal metaplasia显示文摘 | Van Grieken N C T Meijer G A Zur Hausen A | 2003 | J of clinical pathol2003，56，5： | 1 |
| 11 | Solid lipid nanoparticlar (SLN) for controlled drug delivery-drug release and release mechanism 显示文摘 | Zur Muhlen A Schwarz C Mehnert W | 2006 | Eur J pharm Biopharm2006，47，3： | 1 |
| 12 | Serum insulin-like growth factor 1 reference values for an automated chemiluminescence immunoassay system:results from a multicenter study显示文摘 | Brabant G von zur Mühlen A Wüster C | 2003 | Horm Res2003，60，： | 1 |
| 13 | Functional character- ization of HLA-G^+ regulatory T cells in HIV-1 infection显示文摘 | Li C Toth I Schulze Zur Wiesch J | 2013 | PLoS Pathog2013，9，10： | 1 |
| 14 | Evaluation of urine proteome pattern analysis for its potential to reflect coro-nary artery atherosclerosis in symptomatic patients显示文摘 | Von Zur Muhlen C Schiffer E Zuerbig P | | 0，，01： | 1 |
| 15 | Solid lipid nanoparticles(SLN) for controlled drug delivery-drug release and release mechanism显示文摘 | Zur Muhlen A Schwarz C Mehnert W | 1994 | Eur J Pharm Biopharm1994，45，： | 1 |
| 16 | Solid lipid nanoparticles (SLN) for controlled drug delivery: drug release and release mechanism显示文摘 | zur Mtihlen A Schwarz C Mehnert W | 1998 | Eur J Pharm Biopharm1998，45，2： | 1 |
| 17 | Increased expression and immunogenicity of sequence-modified human immunodeficiency virus type 1 gag gene显示文摘 | zur Megede J Chen M C Doe B | 2000 | J Virol2000，74，6： | 1 |
| 18 | Inhibition of tumorigenicity of cervical cancer cells in nude mice by HPV E6E7 antisense RNA显示文摘 | Von Knebel Doeberitz M Rittmiiller C zur Hausen H | 1992 | Int J Cancer1992，51，： | 1 |
| 19 | In vivo magnetic resonance imaging of acute brain inflammation using microparticles of iron oxide显示文摘 | MCATEER M A SIBSON N R von ZUR M C | 2007 | Nature Medicine2007，13，10： | 1 |
| 20 | Two Different One - Dimensional Structural Motifs in the Same Coordination Polymer: a Novel Interpenetration Infinite Ladders by Bundles of Infinite Chains 显示文摘 | Zaman M B Smith M D zur Loye H C | 2001 | Chem Commun2001，，： | 1 |
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