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| 1 | Effect of HCV NS_3 protein on P53 protein expression in hepatocarcinogenesis显示文摘NTRODUCTIONHepatocelularcarcinoma(HCC)isoneofthemostcommonhumancancersintheworld.Recently,theHCVinfectionwasfoundtobeanetiolo... | FENG De Yun, CHEN Rui Xue, PENG Yong, ZHENG Hui and YAN Ya Hui | 1999 | World Journal of Gastroenterology1999，5，1： | 25 |
| 2 | Cellular automata pedestrian movement model considering human behavior显示文摘The pedestrian movement is more complex than vehicular flow for the reason that people are more flexible and intelligent than car. Without the limit of 搇anes?pedestrian movement is loose and free. Furthermore, they are easily affected by other walkers as well as the environ-ment around. In this paper some special technique is intro-duced considering human behavior to make the rules more reasonable. By simulating the two-dimension pedestrian movement, the phase transition phenomena of pedestrian movement, including the up walkers moving from the bottom to the upper boundary and the right walkers moving from the left to the right boundary, are presented. Studying on the effect of the system size on the critical density shows that the critical density is independent of the system size in the scope studied in this paper. | YANG Lizhong, FANG Weifeng, LI Jian, HUANG Rui & FAN Weicheng State Key Laboratory of Fire Science, University of Science and Tech-nology of China, Hefei 230026, China Correspondence should be addressed to Yang Lizhong (e-mail:zxd@ustc. edu.cn) | 2003 | Chinese Science Bulletin2003，48，16： | 16 |
| 3 | Effect of endotoxin on fibronectin synthesis of rat primary cultured hepatocytes显示文摘EfectofendotoxinonfibronectinsynthesisofratprimaryculturedhepatocytesJIAJinBin,HANDeWu,XURuiLing,GAOFei,ZHAOLongFeng,ZHA... | JIA JinBin, HAN DeWu, XU RuiLing, GAO Fei, ZHAO LongFeng, ZHAO YuanChang, YAN JinPin and MA XueHuiKeywords liver cirrhosis endotoxins fibronectin/analysis liver/cytology cells, cultured lipopolysaccharide | 1998 | World Journal of Gastroenterology1998，4，4： | 14 |
| 4 | Effective solutions to a global 3D visual system in networking environments显示文摘The development of Web-based global 3D visual system has made progresses. However, there exist few mature and effective ways for managing, transmitting and visualizing massive spatial data. Based on the related work, the paper illustrates the ellipsoidal quadtree technique for rapid access multi-scale and multiple level geographical data, integrates the streaming with level-of-detail rendering method for transmitting the data on the network, and im-plements large-scale terrain surface simplification using M-band wavelet transforms and mul-tiresolution triangulations. We fulfil a web-based global terrain visual system using COM com-ponents on the basis of the above techniques. The system has a good prospect in the military simulation and city plans. | ZHANG Liqiang1,2, ZHANG Yan3, YANG Chongjun2, LIU Suhong1, REN Yingchao2, RUI Xiaoping2 & LIU Donglin2 1. State Key Laboratory of Remote Sensing Sciences, School of Geography and Remote Sensing, Beijing Normal University, Beijing 100875, China 2. State Key Laboratory of Remote Sensing Sciences, Institute of Remote Sensing Applications, Chinese Academy of Sciences, Beijing 101001, China 3. Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 101001, China | 2005 | Science China Earth Sciences2005，48，11： | 13 |
| 5 | DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments显示文摘The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors,and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses.Through a detailed analysis of the Arabidopsis thaliana genome,82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS),PI-phosphate kinases (PIPK),phospholipases (PL),inositol polyphosphate phosphatases (IPPase),inositol polyphosphate kinases (IPK),PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK,PLC,PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly,DNA chip technology was employed to study the expression patterns of various isoforms.In total,79 mRNA clones were amplified and used for DNA chip generation. Expression profile analysis was performed using samples that represented multiple tissues or cellular responses. Tested samples included normal leaf,stem and flower tissues,and leaves from plants treated with various hormones (auxin,cytokinin,gibberellin,abscisic acid and brassinosteroid) or environmental factors (temperature,calcium,sodium,drought,salicylic acid and jasmonic acid).Results showed that many PI pathway-related genes were differentially expressed under these experimental conditions.In particular,the different isoforms of each family were specifically expressed in many cases,suggesting their involvement in tissue specificity and cellular responses to environmental conditions. This work provides a starting point for functional studies of the relevant PI-related proteins and may help shed light onto the role of PI pathways in development and cellular responses. | Wen Hui LIN, Rui YE, Hui MA, Zhi Hong XU, Hong Wei XUE National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on 'Plant Molecular Physiology and Signal Transduction', 300 Fenglin Road, 200032 Shanghai, China | 2004 | Cell Research2004，14，1： | 12 |
| 6 | Sequencing of p53 mutation in established human hepatocellular carcinoma cell line of HHC4 and HHC15 in nude mice显示文摘INTRODUCTIONAlthoughtheetiopathologyofhumanhepatocelularcarcinoma(hHCC)isstilunknown,alotofevidencestronglysuggestedthatinfec... | YANG Shan Min, ZHOU Hong, CHEN Rui Chuan, WANG Yu Fang, CHEN Fu, ZHANG Chang Gong, ZHEN Yun, YAN Jiang Hua and SU Jin Hua | 1998 | World Journal of Gastroenterology1998，4，6： | 11 |
| 7 | Catalytic performance of Fe_3O_4-CoO/Al_2O_3 catalyst in ozonation of 2-(2,4-dichlorophenoxy) propionic acid,nitrobenzene and oxalic acid in water显示文摘Fe3O4-CoO/Al2O3 catalyst was prepared by incipient wetness impregnation using Fe(NO3) 3·9H2O and Co(NO3) 2·6H2O as the precursors,and its catalytic performance was investigated in ozonation of 2-(2,4-dichlorophenoxy) propionic acid(2,4-DP) ,nitrobenzene and oxalic acid.The experimental results indicated that Fe3O4-CoO/Al2O3 catalyst enabled an interesting improvement of ozonation eciency during the degradation of each organic pollutant,and the Fe3O4-CoO/Al2O3 catalytic ozonation system followed a radical-type mechanism.The kinetics of ozonation alone and Fe3O4-CoO/Al2O3 catalytic ozonation of three organic pollutants in aqueous solution were discussed under the mere consideration of direct ozone reaction and OH radical reaction to well investigate its performance.In the catalytic ozonation of 2,4-DP,the apparent reaction rate constants(k) were determined to be 1.456×10-2 min-1 for ozonation alone and 4.740×10-2 min-1 for O3/Fe3O4-CoO/Al2O3.And O3/Fe3O4-CoO/Al2O3 had a larger Rct(6.614×10-9) calculated by the relative method than O3 did(1.800×10-9) ,showing O3/Fe3O4-CoO/Al2O3 generated more hydroxyl radical.Similar results were also obtained in the catalytic ozonation of nitrobenzene and oxalic acid.The above results demonstrated that the catalytic performance of Fe3O4-CoO/Al2O3 in ozonation of studied organic substance was universal to a certain degree. | Shaoping Tong,Rui Shi,Hua Zhang,Chunan Ma College of Chemical Engineering and Materials Science,State Key Laboratory Breeding Base of Green Chemistry-Synthesis Technology,Zhejiang University of Technology,Hangzhou 310032,China | 2010 | Journal of Environmental Sciences2010，22，10： | 10 |
| 8 | Estimation of net primary productivity in China using remote sensing data显示文摘It is significant to estimate terrestrial net primary productivity (NPP) accurately not only for global change research, but also for natural resources management to achieve sustainable development. Remote sensing data can describe spatial distribution of plant resources better. So, in this paper an NPP model based on remote sensing data and climate data is developed. And 1km resolution AVHRR NDVI data are used to estimate the spatial distribution and seasonal change of NPP in China. The results show that NPP estimated using remote sensing data are more close to truth. Total annual NPP in China is 2.645109 tC. The spatial distribution of NPP in China is mainly affected by precipitation and has the trend of decreasing from southeast to northwest. | SUN Rui, ZHU Qi-jiang (Dept. of Resources and Environment Sciences, Beijing Normal University, Beijing 100875, China) | 2001 | Journal of Geographical Sciences2001，11，1： | 10 |
| 9 | Reduction of VOC emissions by a membrane-based gas absorption process显示文摘A membrane-based gas absorption (MGA) process was evaluated for the removal of volatile organic compounds (VOCs) based on C6H6/N2 mixture. The absorption of C6H6 from a C6H6/N2 mixture was investigated using a hydrophobic polypropylene hollow fiber membrane contactor and the aqueous solution of N-formyl morpholine (NFM) as absorbent. The effects of various factors on the overall mass transfer coefficient was investigated. The experimental results showed that the removal efficiency of C6H6 could reach 99.5% in present studied system. A mathematical model based on resistance-in-series concept was presented to predict the value of overall mass transfer coefficient. The average error between the predicted and experimental values is 7.9%. In addition, conventional packed columns for VOCs removal was also evaluated for comparison. | LI Rui, XU Jun, WANG Lianjun, LI Jiansheng, SUN Xiuyun School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. | 2009 | Journal of Environmental Sciences2009，21，8： | 10 |
| 10 | Screening and evaluation of human single-chain fragment variable antibody against hepatitis B virus surface antigen显示文摘BACKGROUND: Phage display technology has become a vital tool in studies aimed at identifying molecules binding to a specific target. It enables the rapid generation and selection of high affinity, fully human antibody product candidates to essentially any disease target appropriate for antibody therapy. In this study, we prepared the recombinant single-chain fragment variable ( ScFv) antibody to hepatitis B virus surface antigen (HBsAg) by the phage display technology for obtaining a virus-targeting mediator. METHODS: mRNA was isolated from B-lymphocytes from a healthy volunteer and converted into cDNA. The fragment variables of heavy and light chain were amplified separately and assembled into ScFv DNA with a specially constructed DNA linker by polymerase chain reaction. The ScFv DNA was ligated into the phagmid vector pCANT-AB5E and the ligated sample was transformed into competent E. coli TG1. The transformed cells were infected with M13K07 helper phage to form a human recombinant phage antibody library. The volume and recombinant rate of the library were evaluated by bacterial colony count and restriction analysis. After two rounds of panning with HBsAg. the phage clones displaying ScFv of the antibody were selected by enzyme-linked immunosorbant assay ( ELISA) from the enriched phage clones. The antigen binding affinity of the positive clone was detected by competition ELISA. HB2151 E. coli was transfected with the positive phage clone demonstrated by competition ELISA for production of a soluble form of the anti-HBsAg ScFv. ELISA assay was used to detect the antigen binding affinity of the soluble anti-HBsAg ScFv. Finally, the relative molecular mass of soluble anti-HBsAg ScFv was measured by SDS-PAGE. RESULTS: The variable heavy ( VH ) and variable light (VL) and ScFv DNAs were about 340bp, 320bp and 750bp, respectively. The volume of the library was up to 2 × 106 and 8 of 10 random clones were recombinants. Two phage clones could strongly compete with the original HBsAb for binding to HBsAg. Within 2 strong positive phage clones, the soluble anti-HBsAg ScFv from one clone was found to have the binding activity with HBsAg. SDS-PAGE showed that the relative molecular weight of soluble anti-HBsAg ScFv was 32 kDa. CONCLUSION: The anti-HBsAg ScFv successfully produced by phage antibody technology may be useful for broadening the scope of application of the antibody. | Jian-Lin Zhang, Jian-Jin Guo, Zi-Yan Zhang, Yi-Xin Jing, Lin Zhang, Rui Guo, Ping Yan, Niu-Liang Cheng, Bo Niu and Jun Xie Department of Biochemistry and Molecular Biology, Shanxi Medical University ,Taiyuan 030001,China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，2： | 8 |
| 11 | The non-destructive analysis of ancient jade artifacts unearthed from the Liangzhu sites at Yuhang,Zhejiang显示文摘Samples of the jade artifacts unearthed from the Liangzhu sites at Yuhang (Fanshan,Yaoshan, Huiguanshan and Tangshan) of Zhejiang province were described. The chemical composition, phase and jade structure of a hundred and more intact samples were analyzed by the non-destructive techniques of PIXE, XRD and laser Raman spectroscopy. Results showed that the minerals of the jade artifacts mostly belonged to tremolite and tremolite-actinolite. Relationship of the civilization development in the Neolithic age and the application of tremolite type mineral in jade artifacts was discussed. The contents of rare and trace elements in tremolite jade artfacts and in mineral samples were measured for the first time. The possible mineral sources of these tremolite type jade artifacts were discussed. | GAN FuXi1,3, CAO JingYan2, CHENG HuanSheng3, Gu DongHong1, RUI GuoYao2, FANG XiangMing2, DONG JunQing1 & ZHAO HongXia1 1Scientific Archeology Center, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China 2 Research Institute of Cultural Relics and Archeology of Zhejiang, Hangzhou 310014, China 3 Fudan University, Shanghai 200433, China | 2010 | Science China(Technological Sciences)2010，53，12： | 8 |
| 12 | Clinicopathological analysis of 14 patients with combined hepatocellular carcinoma and cholangiocarcinoma显示文摘BACKGROUND:Combined hepatocellular carcinoma and cholangiocarcinoma (cHCC-CC) is an uncommon subtype of primary hepatic carcinoma,and its prognosis is poor.This study was undertaken to investigate the prognosis and the clinicopathological characteristics of cHCC-CC,including their possible cellular origin.METHODS:Among 852 patients with a primary hepatic carcinoma who underwent hepatectomy from January 1998 to April 2008 at our hospital,cHCC-CC was identified in 14 patients The clinicopathological characteristics of the 14 patients were analyzed retrospectively.The expression of the liver stem cell markers (c-kit,CD90,CD133 and CK19) in the tumor tissue was detected by immunohistochemistry,and the Kaplan-Meier method was used to evaluate survival.RESULTS:Among the 14 patients,9 presented with abdominal pain,3 with anorexia and debilitation,and the remaining two patients were asymptomatic.The mean age was 53.6±3.0 (range 38-74) years.Among the included patients,11 had an elevated serum alpha-fetoprotein level,13 were infected with hepatitis B virus,9 had vascular invasion and 1 had lymph node metastasis The average diameter of the tumors was 9.9±1.1 (range 5.0-16.0) cm.The median overall survival time was 7.9±1.0 months In addition,the presence of the liver stem cell markers,c-kit CD90,CD133 and CK19 was 71.4%,85.7%,92.9% and 78.6% respectively.All four markers were simultaneously expressed in eight cases.CONCLUSIONS:cHCC-CC has aggressive characteristics and the prognosis is extremely dismal.The high expression of liver stem cell markers in the tumor tissue suggests that these tumors may derive from liver stem cells. | Xian-Huan Yu,Lei-Bo Xu,Hong Zeng,Rui Zhang,Jie Wang and Chao LiuAuthor Affiliations:Department of Hepato-Pancreato-Biliary Surgery and Department of Pathology,Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou 510120,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，6： | 7 |
| 13 | Diversity and evolution of MicroRNA gene clusters显示文摘microRNA(miRNA) gene clusters are a group of miRNA genes clustered within a proximal distance on a chromosome.Although a large number of miRNA clusters have been uncovered in animal and plant genomes,the functional consequences of this arrangement are still poorly understood.Located in a polycistron,the coexpressed miRNA clusters are pivotal in coordinately regulating multiple processes,including embryonic development,cell cycles and cell differentiation.In this review,based on recent progress,we discuss the genomic diversity of miRNA gene clusters,the coordination of expression and function of the clustered miRNAs,and the evolutionarily adaptive processes with gain and loss of the clustering miRNA genes mediated by duplication and transposition events. | ZHANG YanFeng,ZHANG Rui & SU Bing State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650223,China | 2009 | Science China(Life Sciences)2009，52，3： | 7 |
| 14 | 医学模型分类及世界研发商显示文摘医学模型架起医学理论通往临床实践的桥梁。基础解剖示教模型、局部功能性模型、计算机互动模型、虚拟培训系统、生理驱动模拟系统,这五部分各自具有不同功能,在医学生培养中缺一不可。生理驱动模拟系统再造“真实临床环境”,帮助医学生最终实现临床梦想。 | Academy and marketing Department of Rizheng Huarui Enterprise LuSun Wyatt(Ri Zheng Hua Rui) China Co.,Ltd., No.9, Fangzhuang Nanlu, Fengtai District, Beijing, 100079, PRC | 2007 | 中国医院2007，11，4： | 7 |
| 15 | In situ hybridization assay of androgen receptor gene in hepatocarcinogenesis显示文摘NTRODUCTIONItiswelknownthatsexhormoneplaysanimportantroleinregulationofcelgrowth,organdevelopmentandcarcinogenesis.Butthemost... | ZHAO Guo Qiang, XUE Ling, XU Hong Yu, TANG Xi Ming, HU Rui De and DONG Jun | 1998 | World Journal of Gastroenterology1998，4，6： | 7 |
| 16 | Dynamic fracture toughness of TA15ELI alloy studied by instrumented impact test显示文摘The dynamic fracture toughness of TA15ELI alloy with two types of microstructures was studied by instrumented impact test.Charpy specimens with both the 0.2 mm U-notch and the a/W = 0.2 pre-crack were adopted to compare notch sensitivity in the two microstructures.The result shows that the specimen with Widmanst?tten microstructure exhibits a better dynamic fracture toughness and lower notch sensitivity than that with lath-like microstructure.Fracture surfaces in the case of the two microstructures are analyzed to have a ductile and brittle mixed feature under dynamic loading.The fracture surface of lath-like microstructure is composed of dimples and tear ridges,while that of Widmanst?tten microstructure is covered with rough block-like facets and dimples and tear ridges.The α phase boundaries and α/β interfaces act as locations for void nucleation and crack arrest and deviation.The decrease in width of α phase lamellae leads to the increase in the amount of boundaries and interfaces,which causes the increase in the consumption of impact energy and results in the improvement in dynamic fracture toughness. | LIU Rui,HUI Songxiao,YE Wenjun,XIONG Baiqing,YU Yang,and FU Yanyan State Key Laboratory for Fabrication & Processing of Nonferrous Metals,General Research Institute for Nonferrous Metals,Beijing 100088,China | 2010 | Rare Metals2010，29，6： | 6 |
| 17 | Coupled BEM-FEM Analysis of the Large-Diameter Cylinder Structure System显示文摘A method of coupled BEM-FEM analysis for the elastic spatial structure system is presented. It can be applied to the calculation of the stress and deformation of the large-diamater cylinder structure system and it is suitable for symmetric or non-symmetric structures under the distributed or concentrated load. Numerical examples show that the proposed method and computer program BEFEM are quite efficient in the analysis of the large-diameter cylinder structure problems in ocean engineering. | Zhou Xireng, Duan Xudong, Sang Xuezhu Zhang Rui and Dai Jialu 1 Professor, Tianjin University, Tianjin2 Engineer, Xiamen Port Office, Fujian3 Lecturer, Tianjin University, Tianjin4 Senior Engineer, Tianjin Port Office, Tianjin | 1992 | China Ocean Engineering1992，7，2： | 6 |
| 18 | Analysis of Phthalate Esters in Air, Soil and Plants in Plastic Film Greenhouse显示文摘The phthalate esters such as DMP, DEP, DBP and DEHP in air, soil and plant samples in plastic film greenhouse were clean up with fine silica gel column and determined with HPLC. It was found that the concentrations of PEs in air and soil samples in plastic film greenhouse are much higher than those of contrast samples. But concentrations of PEs in plants in plastic film greenhouse are not remarkably affected by the pollution of air and soil. | Xi Kui WANG*, Wei Lin GUO, Ping Rui MENG, Jia An GAN College of Chemistry and Environmental Science, University of Jinan, Jinan 250022 | 2002 | Chinese Chemical Letters2002，13，6： | 6 |
| 19 | High Expression of NKG2A/CD94 and Low Expression of Granzyme B Are Associated with Reduced Cord Blood NK Cell Activity显示文摘Human umbilical cord blood (CB) has recently been used as a source of stem cells in transplantation. NK cells derived from CB are the key effector cells involved in graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL). It was reported that the activity of CB NK cells was lower than that of adult peripheral blood (PB) NK cells. In this study,we analyzed the expression of some NK cell receptors and cytotoxicity-related molecules in CB and PB NK cells. The expressions of activating NK receptors,CD16,NKG2D and NKp46,did not show significant difference between CB and PB NK cells. But the expression of inhibitory receptor NKG2A/CD94 was significantly higher on CB NK cells. As to the effector function molecules,granzyme B was expressed significantly lower in CB NK cells,but the expressions of intracellular perforin,IFN-γ,TNF-α and cell surface FasL and TRAIL did not show difference between CB and PB NK cells. The results indicated that the high expression of NKG2A/CD94 and low expression of granzyme B may be related with the reduced activity of CB NK cells. | Yanyan Wang1,2,Han Xu1,2,Xiaodong Zheng1,Haiming Wei1,Rui Sun1 and Zhigang Tian1,31Institute of Immunology,Hefei National Laboratory for Physical Materials at Microscale and School of Life Sciences,University of Science and Technology of China,Hefei 230027,China 2Yanyan Wang and Han Xu equally contributed to the present study | 2007 | Cellular & Molecular Immunology2007，4，5： | 5 |
| 20 | Microstructure and oxygen storage capacity of Sr-modified Pt/CeO_2-ZrO_2 catalysts | Rui Ran a,Jun Fan b,Duan Weng a,a State Key Lab of New Ceramic and Fine Processing,Department of Materials Science and Engineering,Tsinghua University,Beijing 100084,China b The Administrative Centre for China’s Agenda 21,Beijing 100038,China | 2012 | Progress in Natural Science:Materials International2012，22，1： | 5 |
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