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| 1 | Interleukin-1α, 6 regulate the secretion of vascular endothelial growth factor A, C in pancreatic cancer显示文摘Vascular endothelial growth factor (VEGF, namely VEGF-A) is an angiogenic polypeptide and VEGF-C is a lymphangiogenic polypeptide that has been implicated in cancer growth, invasion and metastasis. Several cytokines and growth factors play an important part in cancer progression. These cytokines and growth factors are the principal mediators of cancer cells-stromal cell interaction , which is critical for invasion of cancer cells to the surrounding tissues and metastatic dissemination to distant organs. In this study, we studied VEGF-A, C expression in cultured human pancreatic cancer cell lines and whether the presence of VEGF-A, C in the cell lines is regulated by cytokines interleukin-lct (EL-1α), and interleukin-6 (IL-6). METHODS: We used Northern blot and Western blot methods to analyze expression of the gene and protein of VEGF-A, C in all 6 tested cell lines (ASPC-1, CAPAN-1, MIA-PaCa-2, PANC-1, COLO-357 and T3M4) respectively. To analyze what is the regulator for this VEGF-A, C expression in pancreatic cancer,we used the reverse transcription -polymerase chain reaction (RT-PCR) method to analyze VEGF-A, C expression in cultured human pancreatic cancer cell lines (CAPAN-1 and COLO-357) under the stimulation with IL-1α (10μg/L) or IL-6 (100 μg/L). RESULTS:Northern blot analysis revealed the presence of the 4.1-kb VEGF-A mRNA transcript and 2.4-kb VEGF-C mRNA transcript in all 6 tested cell lines. Immunoblotting with highly specific anti-VEGF-A, anti-VEGF-C antibody revealed the presence of a molecular weight of 43-kDa VEGF-A protein and 55-kDa VEGF-C protein in all the cell lines. RT-PCR analysis revealed the levels of the VEGF-A and VEGF-C gene were 1-2 fold and a 1-fold increase in the COLO-357 cell line by stimulation with IL-la, however, no effect was found in the CAPAN-1 cell line. The levels of the VEGF-A and VEGF-C gene were 2-5 fold and a 1-fold increase in the CAPAN-1 cell line by stimulation with IL-6, but, no effect was found in the COLO-357 cell line. CONCLUSION:These findings suggested that the expression of VEGF-A, C and their regulation by IL-1α, IL-6 in pancreatic cancer contributes to the lymphatic and distant metastasis and the disease progression. | Department of Hepatobiliary Surgery (Tang RF, Zhang FR, Peng L, Wang SX, Xiao Y and Zhang M) and Department of Dermatology (Wang SX), 4th Hospital, Hebei Medical University, Shijiazhuang 050011, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 6 |
| 2 | Brain changes in diabetes mellitus patients withgastrointestinal symptoms显示文摘Diabetes mellitus is a common disease and its prevalence is increasing worldwide. In various studies up to 30%-70% of patients present dysfunction and complications related to the gut. To date several clinical studies have demonstrated that autonomic nervous system neuropathy and generalized neuropathy of the central nervous system(CNS) may play a major role. This systematic review provides an overview of the neurodegenerative changes that occur as a consequence of diabetes with a focus on the CNS changes and gastrointestinal(GI) dysfunction. Animal models where diabetes was induced experimentally support that the disease induces changes in CNS. Recent investigations with electroencephalography and functional brain imaging in patients with diabetes confirm these structural and functional brain changes. Encephalographic studies demonstrated that altered insular processing of sensory stimuli seems to be a key player in symptom generation. In fact one study indicated that the more GI symptoms the patients experienced, the deeper the insular electrical source was located. The electroencephalography was often used in combination with quantitative sensory testingmainly showing hyposensitivity to stimulation of GI organs. Imaging studies on patients with diabetes and GI symptoms mainly showed microstructural changes,especially in brain areas involved in visceral sensory processing. As the electrophysiological and imaging changes were associated with GI and autonomic symptoms they may represent a future therapeutic target for treating diabetics either pharmacologically or with neuromodulation. | Anne M Drewes Eirik Søfteland Georg Dimcevski Adam D Farmer Christina Brock Jens B Frøkjær Klaus Krogh Asbjørn M Drewes | 2016 | World Journal of Diabetes2016，7，2： | 4 |
| 3 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014，20，12： | 3 |
| 4 | Multiband emission from single β-NaYF_(4)(Yb,Er) nanoparticles at high excitation power densities and comparison to ensemble studies显示文摘Ensemble and single particle studies of the excitation power density (P)-dependent upconversion luminescence (UCL) of core and core-shell β-NaYF_(4):Yb,Er upconversion nanoparticles (UCNPs) doped with 20% Yb^(3+) and 1% or 3% Er^(^(3+)) performed over a P regime of 6 orders of magnitude reveal an increasing contribution of the emission from high energy Er^(3+) levels at P > 1 kW/cm^(2). This changes the overall emission color from initially green over yellow to white. While initially the green and with increasing P the red emission dominate in ensemble measurements at P < 1 kW/cm^(2), the increasing population of higher Er^(^(3+)) energy levels by multiphotonic processes at higher P in single particle studies results in a multitude of emission bands in the ultraviolet/visible/near infrared (UV/vis/NIR) accompanied by a decreased contribution of the red luminescence. Based upon a thorough analysis of the P-dependence of UCL, the emission bands activated at high P were grouped and assigned to 2–3, 3–4, and 4 photonic processes involving energy transfer (ET), excited-state absorption (ESA), cross-relaxation (CR), back energy transfer (BET), and non-radiative relaxation processes (nRP). This underlines the P-tunability of UCNP brightness and color and highlights the potential of P-dependent measurements for mechanistic studies required to manifest the population pathways of the different Er^(3+) levels. | Florian Frenzel Christian Würth Oleksii Dukhno Frédéric Przybilla Lisa MWiesholler Verena Muhr Thomas Hirsch Yves Mély Ute Resch-Genger | 2021 | Nano Research2021，14，11： | 3 |
| 5 | Poly ADP-ribosylation:a DNA break signal mechanism显示文摘 | Althaus FR Kleczkowska HE Malanga M | | 0，，： | 2 |
| 6 | 钾通道J亚家族成员5基因嵌合导致双侧肾上腺皮质增生进而引起的早期原发性醛固酮增多症显示文摘钾通道J亚家族成员5(potassium voltage-gated channel subfamily J member 5,KCNJ5)的体细胞变异最易导致原发性醛固酮增多症(primary aldosteronism,PA),有部分患者因KCNJ5钾通道基因的生殖细胞突变而患该病[家族性醛固酮增多症Ⅲ型(familial hyperaldosteronismⅢ,FH-Ⅲ)]。方法:1998年,美国国立卫生研究院临床中心发现一名11岁的白人男孩,患有早期严重高血压。 | Maria AG Suzuki M Berthon A Kamilaris C Demidowich A Lack J Zilbermint M Hannah-Shmouni F Faucz FR Stratakis CA 周卫(译) 叶鹏(审校) | 2020 | 中华高血压杂志2020，28，12： | 2 |
| 7 | Interaction of the major inflammatory bowel disease susceptibility alleles in Crohn’s disease patients显示文摘AIM:To investigate the interaction of interleukin-23 receptor(IL23R)(rs1004819 and rs2201841),autophagy-related 16-like 1(ATG16L1)(rs2241880), caspase recruitment domain-containing protein 15 (CARD15)genes,and IBD5 locus in Crohn's disease(CD) patients. METHODS:A total of 315 unrelated subjects with CD and 314 healthy controls were genotyped.Interactions and specific genotype combinations of a total of eight variants were tested.The variants of IBD5locus(IGR2198a_1 rs11739135 and IGR2096a_1 rs12521868),CARD15(R702W rs2066845 and L1007fs rs2066847),ATG16L1(rs2241880)and IL23R (rs1004819,rs2201841)genes were genotyped by PCR-RFLP,the G908R(rs2066844)in CARD15 was determined by direct sequencing. RESULTS:The association of ATG16L1 T300A with CD was confirmed[P=0.004,odds ratio(OR)=1.69, 95%CI:1.19-2.41],and both IL23R variants were found to represent significant risk for the disease(P= 0.008,OR=2.05,95%CI:1.20-3.50 for rs1004819 AA;P<0.001,OR=2.97,95%CI:1.65-5.33 for rs2201841 CC).Logistic regression analysis of pairwise interaction of the inflammatory bowel disease (IBD)loci indicated that IL23R,ATG16L1,CARD15 and IBD5(IGR2198a_1)contribute independently to disease risk.We also analysed the specific combina- tions by pair of individual ATG16L1,IL23R rs1004819, rs2201841,IGR2198a_1,IGR2096a_1 and CARD15 genotypes for disease risk influence.In almost all cases,the combined risk of susceptibility pairs was higher in patients carrying two different risk-associated gene variants together than individuals with just one polymorphism.The highest OR was found for IL23R rs2201841 homozygous genotype with combination of positive CARD15 status(P<0.001,OR=9.15,95% CI:2.05-40.74). CONCLUSION:The present study suggests a cumulative effect of individual IBD susceptibility loci. | Veronika Csngei Luca Járomi EnikSáfrány Csilla Sipeky Lili Magyari Bernadett Faragó Judit Bene Noémi Polgár Lilla Lakner Patrícia Sarlós Márta Varga Béla Melegh | 2010 | World Journal of Gastroenterology2010，16，2： | 2 |
| 8 | The role of the hemostatic system in tumor growth, metastasis, and angiogenesis: tissue factor is a bifunctional molecule capable of inducing both fibrin deposition and angiogenesis in cancer显示文摘 | Rickles FR Shoji M Abe K | 2001 | Inter J Hematol2001，73，2： | 1 |
| 9 | A population of very small embryonic-like (VSEL) CXCR4 ( + ) SSEA-1 (-) Oct-4 stem ceils identified in adult bone marrow 显示文摘 | Kucia M Reca R Campbell FR | 2006 | Leukemia2006，20，： | 1 |
| 10 | Corticosteroid effects on blood gene expression in duchenne muscular dystrophy显示文摘 | Lit L Sharp FR Apperson M | 2009 | Pharmacogenomics J2009，9，6： | 1 |
| 11 | RANTES-induced chemokines induce chemokines cascade in dendritic cells显示文摘 | Fischer FR Luo Y Luo M | 2001 | J Immunol2001，167，6： | 1 |
| 12 | NF-κB in cancer:from innocent bystander to major culprit显示文摘 | Karin M Cao Y Greten FR | 2002 | Nature Reviews2002，2，： | 1 |
| 13 | IL-2,its receptors,and bcl-2 and bax genes in normal,hyperplastic and carcinomatous human prostates:immunohistochemical comparative analysis显示文摘 | Royuela M De Miguel MP Bethencourt FR | 2000 | Growth Factors2000，18，： | 1 |
| 14 | Preparation ofporous polymer monoliths featuring enhanced surface cov-erage with gold nanoparticles显示文摘 | Lv Y Alejandro F M Fr^chet J M | 2012 | J Chromatogr A2012，1261，： | 1 |
| 15 | A National Strategic Change in Treatment Policy for Rectal Cancer—Implementation of Total Mesorectal Excision as Routine Treatment in Norway. A National Audit显示文摘 | Arne Wibe M.D. Bj?rn M?ller M.Sc. Jarle Norstein M.D. Erik Carlsen M.D. Ph.D. Johan N. Wiig M.D. Ph.D. Richard J. Heald F.R.C.S. Fr?ydis Langmark M.D. Helge E. Myrvold M.D. Ph.D. Odd S?reide F.R.C.S | 2002 | Diseases of the Colon & Rectum2002，，7： | 1 |
| 16 | Funding systems for higher education and their impacts on institutional strategies and academia:A comparative perspective显示文摘 | Frlich N Schmidt E K Rosa M J | 2010 | International Journal of Educational Management2010，24，1： | 1 |
| 17 | Proteomics of a new esophageal cancer cell line established from Persian patient 显示文摘 | Moghanibashi M Jazii FR Soheili ZS | 2012 | Gene2012，500，1： | 1 |
| 18 | Immunity,inflammation,and cancer显示文摘 | Grivennikov SI Greten FR Karin M | | 0，，06： | 1 |
| 19 | Health risk assessment of dioxin emissions from municipal waste incinerators: the Neerlandquarter (Wilrijk, Belgium)显示文摘 | J Nouwen C Cornelis R De Fré M Wevers P Viaene C Mensink J Patyn L Verschaeve R Hooghe A Maes M Collier G Schoeters R Van Cleuvenbergen P Geuzens | 2001 | Chemosphere2001，，4： | 1 |
| 20 | Is the development of myocardial tolerance to repeated ischemia in humans due to preconditioning or to collateral recruitment显示文摘 | Fleisch M Eberli FR | 1999 | J Am Coll Cardiol1999，33，： | 1 |
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