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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Phase formation in YAG powder synthesized by chemical methods 显示文摘 | SIM S M KELLER K A | 2000 | J Mater Sci2000，35，： | 2 |
| 3 | The spherulitic and lamellar morphology of melt-crystallized isotactic polypropylene 显示文摘 | D R Norton A Keller | 1985 | Polymer1985，26，： | 2 |
| 4 | Solidstate low-loss intracavity saturable absorber for Nd:YLF lasers:An A-FPSA显示文摘 | KELLER U MILLER D A B BOYD G D | 1992 | Opt Lett1992，17，7： | 2 |
| 5 | 空化现象及其模型试验预测的可靠性显示文摘预测水力机械和装置中绕流构件的空化特性,是在空化试验设备中进行模型试验的目的,然而尽管采用了谨慎的测试方法和半经验的比尺关系,试验结果的分散性仍然较大。近年来,在对空化初生的比尺效应进行大规模试验研究的过程中,试验液体的抗拉强度受到特别重视。试验清楚地表明,在未考虑水质的情况下,空化试验结果,尤其是初生空化试验的结果分散性较大,据此既不可能确定绕流物体的特征值,也不可能推导出初生空化的比尺关系。通过精确控制空化试验中试验用水的抗拉强度,可以清楚地得出普遍适用的速度、尺寸和粘性比尺影响的简单关系。 | Keller,A 胡苏萍 | 1994 | 水电站设计1994，10，4： | 2 |
| 6 | EPR evidence of Jahn-Teller polaron formation in La1-xCaxMnO3+y 显示文摘 | Shengelaya A ZHAO Guo-meng Keller H | 1996 | Phys Rev Lett1996，77，： | 2 |
| 7 | The Problem of Gaseous Emissions of Mercmy in Cement Plants显示文摘 | Weisweiler W Keller A | 1992 | Zement-Kalk-Gips1992，45，10： | 1 |
| 8 | Consumer evaluation of brand extension显示文摘 | Aaker D A Keller K L | 1990 | Journal of Marketing1990，23，6： | 1 |
| 9 | Diluteacid hydrolysis of softwoods显示文摘 | Ngyuen Q A Tucker M P Keller F | 1999 | Applied Biochemistry and Biotechnology1999，77，13： | 1 |
| 10 | Inhibition of endothelin-1-mediated up-regulation of iNOS by bosentan ameliorates endotoxin-induced liver injury in cirrhosis 显示文摘 | Keller S Karaa A Paxian M | 2006 | Shock2006，25，3： | 1 |
| 11 | Long-term performance summary for the boot wetland treatment system 显示文摘 | Martin R J Keller H C Clarke A R | 2001 | Water Science and Technology2001，44，1112： | 1 |
| 12 | Antisense inhibition of the GDP-mannose pyrophosphorylase reduces the ascorbate content in transgenic plants leading to developmental changes during senescens显示文摘 | KELLER R SPRINGER F RENZ A | 1999 | Plant J1999，19，： | 1 |
| 13 | The national depressive and manic-depressive association consensus statement on the undertreatment of depression显示文摘 | HIRSCHFELD R M A KELLER M PANICO S | 1997 | JAMA1997，277，： | 1 |
| 14 | The importance of environmental heterogeneity for species diversity and assemblage structure in Bornean stream frogs显示文摘 | Keller A Rodel M O Linsenmair K E | 2009 | Journal of Animal Ecology2009，78，2： | 1 |
| 15 | Pressure ulcers in intensive care patients:Areview of risks and prevention显示文摘 | Paul JB Keller A | 2002 | Intensive Care Med2002，28，： | 1 |
| 16 | Randomized phase 1I trial of capecitabine plus irinotecan(Caplri) vscapecitabine plus oxaliplatin (CapOx) as first-line therapy of advanced colorectal cancer(ACRC) 显示文摘 | Grothey A Jordan K Keller O | 2003 | Proc Am Soc Clin Oncol2003，22，： | 1 |
| 17 | Permanent breast seed implant dosimetry quality assurance显示文摘 | Keller BM Ravi A Sankreacha R | 2012 | Int J Radiat Oncol Biol Phys2012，83，1： | 1 |
| 18 | Identification of novel SNPs in glioblastoma using targeted resequencing 显示文摘 | KELLER A HARZ C MATZAS M | 2011 | PLoS One2011，6，18： | 1 |
| 19 | Rate minimum in solution crystallization of long paraffins 显示文摘 | ORGAN S J UNGAR G KELLER A | 1989 | Macromolecules1989，，22： | 1 |
| 20 | Sensor: A tool for the simulation of hyperspectral remote sensing systems显示文摘 | Borner A Wiest L Keller P | 2001 | ISPRS Journal of Pho- togrammetry and Remote Sensing2001，55，56： | 1 |
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