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| 1 | Expression of p53 and C-myc genes and its clinical relevance in the hepatocellular carcinomatous and pericarcinomatous tissues显示文摘AIM: To investigate the possible roles of p53 and C-mycgenes in the primary hepatocellular carcinogenesis and therelationship between the liver hyperplastic nodule(LHN) andhepatocellular carcinoma(HCC).METHODS: The expression of p53 and C-myc genes wasdetected immunohist-ochemically in 73 and 60 cases of HCCand pericarcinomatous tissues, respectively .RESULTS: The positive expression of p53 in HCC wassignificantly higher than that in pericarcinomatous tissues(P<0.05). In pericarcinomatous tissues, the p53 expressionwas observed only in LHN, but not in liver cirrhosis (LC) andnormal liver tissues. The positive expression rate of C-mycin HCC or LHN was significantly higher than that in LC ornormal liver tissues (P<0.05 and P<0.01), however, nosignificant difference was found between HCC and LHN(P>0.05). The positive expression rate of p53 and C-myc inHCC was correlated with the histological differentiation, thatin the poorly differentiated was significantly higher than thatin well differentiated samples (P<0.05).CONCLUSION: The overexpression of p53 and C-myc genesmight play a role in the carcinogenesis of HCC; And LHNseems a preneoplastic lesion related to hepatocarcinogenesis;No evidence supports that LC contribute directly to thehepatocarcinogenesis. | Zhao-Shan Niu Bo-Kian Li Department of Pathology,Medical College of Qingdao University,Qingdao 266021,Shandong Province,China Mei Wang Department of Foreign languages,Qingdao institute of Architecture and Engineering,Qingdao 266033,Shandong Province,China | 2002 | World Journal of Gastroenterology2002，8，5： | 30 |
| 2 | 二乙基亚硝胺所诱导大鼠肝癌表达上调的基因显示文摘目的:首次大范围地观察二乙基亚硝胺(DEN)诱导的大鼠肝癌组织基因表达的差异,探索DEN诱发肝癌的分子机制.方法:DEN诱导大鼠肝癌,常规抽提和纯化RNA,采用AffymetrixRat230AGeneChip及技术比较肝癌组织与正常大鼠肝组织基因表达的差异.结果:在芯片的15710个基因中,肝癌有84.54%的基因阳性表达,肝癌基因表达在正常肝脏5倍以上的有509个,其中325个为EST片段,已知基因184个,其中的168个基因可以检索到有关文献的报道.在这168个基因中,有100个基因被发现与肿瘤有关,其中有36个与肝癌有关;有4个基因与肝脏有关;另有64个基因与肿瘤和肝脏无关.结论:?DEN诱发大鼠肝癌的后基因组变化中168个基因值得优先关注. | 阚卫兵 方肇勤 管冬元 赵晓珍 吴中华 盛学仕 高必峰 | 2005 | 世界华人消化杂志2005，13，20： | 22 |
| 3 | P53 immunohistochemical scoring:an independent prognostic marker for patients after hepatocellular carcinoma resection显示文摘AIM: To confirm if p53 mutation could be a routinepredictive marker for the prognosis of hepatocellularcarcinoma (HCC) patients.METHODS: Two hundreds and forty-four formalin-fixedparaffin-embedded tumor samples of the patients with HCCreceiving liver resection were detected for nuclearaccumulation of p53. The percent of P53 immunoreactivetumor cells was scored as 0 to 3 + in P53 positive region ( <10% -, 10-30 % +, 31-50 % + +, > 50 % + + + ).Proliferating cell nuclear antigen (PCNA) and someclinicopathological characteristics, including patients' sex,preoperative serum AFP level, tumor size, capsule,vascular invasion (both visual and microscopic ), andEdmondson grade were also evaluated.RESULTS: In univariate COX harzard regression modelanalysis, tumor size, capsule status, vascular invasion,and p53 expression were independent factors that wereclosely related to the overall survival (OS) rates of HCCpatients. The survival rates of patients with 3 + for P53expression were much lower than those with 2 + or + forP53 expression. Only vascular invasion ( P < 0.05 ) andcapsule ( P < 0.01 ) were closely related to the disease-freesurvival (DFS) of HCC patients. In multivariate analysis, p53overexpression (RI 0. 5456, P < 0. 01 ) was the mostsignificant factor associated with the OS rates of patientsafter HCC resection, while tumor size (RI 0.5209, P< 0.01),vascular invasion (RI 0.5271, P < 0.01 ) and capsule (RI-0.8691, P<0.01) were also related to the OS. However, onlytumor capsular status was an independent predictive factor( P< 0.05) for the DFS. No significant prognostic value wasfound in PCNA-LI, Edmondson's grade, patients' sex andpreoperative serum AFP level.CONCLUSION: Accumulation of p53 expression, as well estumor size, capsule and vascular invasion, could bevaluable markers for predicting the prognosis of HCCpatients after resection. The quantitativeimmunohistochemical scoring for P53 nuclear accumulationmight be more valuable for predicting prognosis of patientsafter HCC resection than the common qualitative analysis. | Lun-Xiu Qin Zhao-You Tang Zeng-Chen Ma Zhi-Quan Wu Xin-Da Zhou Qing-Hai Ye Yuan Ji Li-Wen Huang Hu-Liang Jia Hui-Chuan Sun Lu Wang,Liver Cancer Institute and Zhongshan Hospital,Fudan University,Shanghai,China | 2002 | World Journal of Gastroenterology2002，8，3： | 18 |
| 4 | Potent inhibition of angiogenesis and liver tumor growth by administration of an aerosol containing a transferrin-liposome-endostatin complex显示文摘AIM: To obtain an efficient delivery system for transportingendostatin gene to mouse liver tumor xenografts byadministration of aerosol.METHODS: Recombinant plasmid pcDNA3.0/endostatincontaining human endostatin gene together with signalpeptide from alkaline phosphatase were transferred intohuman umbilical vein endothelial cell (HUVEC) by transfenin(TF)-liposome-endostatin complex. Western blot was usedto detect the expression of human endostatin in transfectedHUVEC cells and its medium. After the tumor-bearing micewere administrated with TF-liposome-endostatin complex,the lung tissue was analyzed by immunohistochemicalmethod for expression of endostatin and the tumors weretreated with CD-31 antibody to detect the density ofmicrovesseles in tumor tissues. The inhibition of tumorgrowth was estimated by the weight of tumors from groupstreated with different dos es of TF-liposome-endostatincomplex. DNA fragmentation assay was used to detect theapoptosis of the cells from primary liver tumor.RESULTS: Western blot analysis and immunohistochemicalmethod confirmed the expression of endostatin proteininvitro and in vivo. After the tumor sections were treated withCD-31 antibody, the positive reaction cells appeared brownwhile the negative cells were colorless. The positively stainedarea of the TF-liposome-endostatin treated group wassignificantly smaller (P<0.01, 645.8+55.2 μm2) than that ofthe control group (1325.4+198.5 μm2). The data showed asignificant inhibition of angiogenesis. After administrationof TF-liposome-endostatin, comparing with the control groupadministrated with TF-liposome-pcDNA3.0, liver tumorgrowth in the mice treated with 50, 250 and 500 mg DNA/kg was inhibited by 36.6 %, 40.8 %, and 72.8 %, respectively(P<0.01). And a typical DNA fragmentation of apoptosis wasfound in the cells from tumor tissues of the mice treatedwith TF-liposome-endostatin but none in the control group.CONCLUSION: Endostatin gene could be efficientlytransported into the mice with TF-liposome-DNA deliverysystem by administration of aerosol. TF-liposome-mediatedendostatin gene therapy strongly inhibited angiogenesis andthe growth of mouse xenograft liver tumors. It also couldpromote the development of apoptosis of tumors withoutdirect influence on tumor cells. | XiLi Geng-FengFu Yan-RongFan Chan-FuShi Xin-JuanLiu Gen-XingXu Jian-JunWang | 2003 | World Journal of Gastroenterology2003，9，2： | 14 |
| 5 | Preparation of monoclonal antibody against apoptosis-associated antigens of hepatoma cells by subtractive immunization显示文摘AIM: To elucidate the expression of the apoptosis-associatedmolecules in human primary hepatocellular carcinoma (HCC)cells, and prepare the monoclonal antibodies (mAb) againstthe apoptosis-associated antigens of HCC cells.METHODS: Human HCC cell line HCC-9204 cells wereinduced apoptosis with 60 mL.L-1 ethanol for 6 h and theirmorphological changes were observed by transmissionelectron microscope. The cell DNA fragmentations weredetected by Terminal Deoxynucleotidyl transferase-mediateddUTP nick end labeling (TUNEL) assay, and the cell DNAcontents by flow cytometry. Ten mice were immunized withethanol-induced apoptotic HCC-9204 cells with the methodof subtractive immunization, while the other 10 mice usedas the control were immunized by the routine procedures.The tail blood of all the mice were prepared after the lastimmunization, and the produced antibodies were determinedby the immunocytochemical ABC staining. The splenic cellsof the mice whose tail blood sera-HCC-9204 cells serumreactions were most different between the apoptotic andthe non-apoptotic were prepared and fused with the mousemyeloma cell line SP2/0 cells. The positive antibodies wereselected by ELISA assay. The fusion rates of hybridoma cellsand the producing rates of antibodies were calculated. Thefused cells that secreted candidate objective antibody werecloned continually with the of limited dilution method, andthen selected and analyzed further by theimmunocytochemical ABC staining. The chromosomes of thecloned hybridoma cells that secreted objective mAb and themAb immunoglobulin (Ig) subtype of the prepared mAb werealso determined. The molecular mass of the mAb associatedantigen was analyzed by Western blot assay.RESULTS: HCC-9204 cells treated with 60 mL.L-1 ethanolfor 6 h, manifested obvious apoptotic morphological changes,the majority of the cells were TUNEL-positive, and the sub-G1 apoptotic peak was evident. There were 2 mice in theexperimental group whose tail blood serum reacted stronglywith the apoptotic HCC-9204 cells, but weakly with theirnon-apoptotic counterparts. In the fusion rates of hybridomacells as well as the producing rates of the antibody deseribedabove, there did not show significant difference betweenthe experimental and the control group, but weakly withnon-apoptotic HCC-9204. However, the total producing rateof antibodies in the experimental group was significantlylower compared with the control (P<0.01), and so was theproducing rate of the antibodies which reacted strongly withboth apoptotic and non-apoptotic HCC-9204 cells(P<0.01).After cloned continually for several times the cell that producemAb which reacted strongly with the nuclei of ethanol-induced apoptotic HCC-9204 cells, but very weakly with thatof non-apoptotic cells was selected out. Chromosome analysisrevealed that the selected cell was with the universalcharacteristics of the monoclonal hybridoma cells whichsecreted mAb, and the Ig subtype of the prepared mAb wasIgG1. The molecular mass of this mAb associated antigenof was about 75 ku.CONCLUSION: Subtractive immunization is a useful methodto prepare the mAb against the apoptosis-associated antigensof cells. The expression of some molecules increases to someextent in HCC-9204 cells in the process of apoptosis inducedby Iow-concentration ethanol. The mAb that may be againstethanol-induced apoptosis-associated antigens of HCC cellswas successfully prepared and primarily identified. | Lian-Jun Yang Wen-Liang Wang Department of Pathology,Institute of Cancer Research,The Fourth Military Medical University (FMMU),Xian 710032,Shaanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，5： | 13 |
| 6 | IGF-Ⅰ、IGF-Ⅱ及IGF-ⅠR在前列腺癌中的表达和意义显示文摘目的研究Ⅰ型胰岛素样生长因子(IGF-Ⅰ)、Ⅱ型胰岛素样生长因子(IGF-Ⅱ)和胰岛素样生长因子Ⅰ受体(IGF-ⅠR)在中国患者前列腺癌组织中的表达情况及其与前列腺癌生物学行为的关系。方法应用免疫组织化学法检测IGF-Ⅰ、IGF-Ⅱ和IGF-ⅠR在良性前列腺增生及前列腺癌组织中的表达情况,实验所得数据用SPSS 12.0统计软件处理。计数资料采用直接概率法和四格表x^2检验,当n>40但有1≤T<5时,用校正的卡方检验,P<0.05为差异有显著性的标准。Spearman等级相关方法分析免疫组化结果与临床病理因素的关系。结果IGF-Ⅰ在良性前列腺增生和前列腺癌组织中的阳性表达率分别为26.67%(8/30)、65.12%(28/ 43)(P<0.05);IGF-Ⅱ在良性前列腺增生和前列腺癌组织中的表达率分别为36.67%(11/30)、72.09%(31/ 43)(P<0.05);IGF-ⅠR在良性前列腺增生和前列腺癌组织中的表达率分别为:43.33%(13/30)、79.07% (34/43)(P<0.05)。结论IGF-Ⅰ、IGF-Ⅱ和IGF-ⅠR与前列腺癌的发生相关,IGF-Ⅰ、IGF-Ⅱ与前列腺癌的发展呈正相关,IGF-ⅠR与前列腺癌的发展不相关。 | 罗玉华 翁志梁 蔡健 李湘斌 | 2009 | 中国男科学杂志2009，23，1： | 11 |
| 7 | 牛磺酸对大鼠肝星状细胞的影响显示文摘目的探讨天然牛磺酸对大鼠肝星状细胞的影响。方法采用HSC-T6细胞株为模式细胞,传代培养,MTT法观察天然牛磺酸对HSC-T6细胞增殖的影响,胶原晶格收缩法观察其对HSC-T6细胞收缩的影响,ELISA法观察其对HSC-T6细胞分泌I型胶原(CoI)、纤维连接蛋白(FN)、转化生长因子-β1(TGF-β1)的影响。结果天然牛磺酸中、高剂量组对HSC-T6细胞抑制率分别为29·72%,33·04%,与对照组相比差异具有统计学意义(P<0·01);各剂量组较对照组均能显著抑制HSC-T6收缩(P<0·05或P<0·01),抑制作用与剂量呈依赖关系;中、高剂量组较对照组相比能显著抑制HSC-T6分泌CoI、FN、TGF-β1(P<0·05或P<0·01)。结论天然牛磺酸可抑制HSC-T6增殖、收缩、分泌CoI、FN、TGF-β1。 | 梁健 邓鑫 吴金玉 杨光业 黄仁彬 | 2006 | 广西医学2006，28，1： | 9 |
| 8 | Expression of liver cancer associated gene HCCA3显示文摘AIM: To study and clone a novel liver cancer reisted gene,and to explore the molecular basis of liver cancer genesis.METHODS: Using mRNA differential display polymerasechain reaction (DDPCR), we investigated the difference of mRNA in human hepatocellular carcinoma (HCC) and paired surrounding liver tissues, and got a gene probe. By screening a human placenta cDNA library and genomic homologous extend, we obtained a full-length cDNA named HCCA3. We analyzed the expression of this novel gene in 42pairs of HCC and the surrounding liver tissues, and distribution in human normal tissues by means of Northern blot assay.RESULTS: A full-length cDNA of liver cancer associated gene HCCA3 has been submitted to the GeneBank nucleotide sequence databases ( Accession No. AF276707 ). The positive expression rate of this gene was 78.6% (33/42) in HCC tissues, and the clinical pathological data showed that the HCCA3 was closely associated with the invasion of tumor capsule ( P = 0.023) and adjacant small metastasis satellite nodules lesions ( P= 0.041). The HCCA3 was widely distributed in the human normal tissues, which was intensively expressed in lungs, brain and colon tissues,while lowly expressed in the liver tissues.CONCLUSION: A novel full-length cDNA was cloned and differentiated, which was highly expressed in liver cancer tissues. The high expression was closely related to the tumor invasiveness and metastasis, that may be the late heredited change in HCC genesis. | Zheng-Xu Wang~1 Gui-Fang Hu~1 Hong-Yang Wang~2 Meng-Chao Wu~2 1 Department of General Surgery,Chinese PEA General Hospital of Lanzhou Military Command,Lanzhou 730050,Gansu Province,China2 Eastern Hepatobilliary Surgical Hospital,Second Military Medical University,Shanghai 200438,China | 2001 | World Journal of Gastroenterology2001，7，6： | 9 |
| 9 | Clinical significance of serum IGF-I,IGF-II and IGFBP-3 in liver cirrhosis显示文摘AIM: To investigate the relationship between insulin-like growth factor-Ⅰ, -Ⅱ (IGF-Ⅰ and IGF-Ⅱ), IGF-binding protein 3(IGFBP-3) and Child-Pugh score in patients with liver cirrhosis,and to search for potential clinical markers of liver function.METHODS: Forty-four patients with advanced liver cirrhosis of viral origin were divided into 3 groups according to severity of cirrhosis (Child-Pugh score) and 38 healthy subjects served as controls. Serum levels of IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 were measured by immunoradiometric assay.RESULTS: Serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 levels were significantly lower in patients with cirrhosis than in controls,and serum concentrations of IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 were associated with the severity of liver dysfunction, and dropped sharply during the progression of liver failure. Among these 3 parameters, serum IGF-Ⅱ was the most sensitive and effective indicator for liver dysfunction. Concentrations of IGF-Ⅰ <30 ng/mL, IGF-Ⅱ <200 ng/mL and IGFBP-3 <6 ng/mL implied a negative prognosis for patients with liver cirrhosis.CONCLUSION: Serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 may provide a new dimension in the assessment of liver dysfunction.Combined detection of serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 with Child-Pugh score is more effective in predicting prognosis than Child-Pugh score alone. | Yun-LinWu JingYe ShuZhang JieZhong Rong-PingXi | 2004 | World Journal of Gastroenterology2004，10，18： | 7 |
| 10 | 肝硬化大鼠门静脉高压症不同手术方式对其肝脏的影响显示文摘目的:评价门奇断流术(PAD)、肠腔分流术(MCS)、远端脾腔分流术(DSCS)对门体分流率(PSS)、肝脏功能、线粒体功能和抗氧化能力的影响,为合理选择手术方式提供理论依据. 方法:用CCl4/乙醇诱导大鼠肝硬化门脉高压症模型,观察了不同术式(MCS、DSCS、PAD)的死亡率及手术前后肝功能、门体分流率(PSS)、肝细胞线粒体功能和超微结构及肝组织SOD活性、巯基水平、LPO含量的变化,并探讨了线粒体功能与抗氧化能力的内在关系. 结果:肝硬化门脉高压时,PSS远高于正常,肝细胞线粒体功能、抗氧化能力均下降;肠腔分流(MCS)组术后肝细胞线粒体功能、抗氧化能力进一步下降且恢复慢;选择性远端脾腔分流术(DSCS)组和门奇断流术(PAD)组的上述指际变化小且恢复较快,其中DSCS组恢复更快且死亡率低. 结论:选择性分流术(DSCS) | 徐新保 蔡景修 董家鸿 何振平 韩本立 冷希圣 | 2004 | 世界华人消化杂志2004，12，3： | 5 |
| 11 | 血管紧张素Ⅱ与肝纤维化研究进展显示文摘 | 谢红东 刘成海 | 2008 | 临床肝胆病杂志2008，24，3： | 4 |
| 12 | 肝素结合生长因子Midkine在早期大肠肿瘤的表达显示文摘 | 吴晓冬 杨绍娟 朱辉 王维忠 | 2006 | 中国肿瘤临床2006，33，7： | 3 |
| 13 | 免疫缺陷小鼠肿瘤模型的建立及其肿瘤相关免疫机制的探讨显示文摘目的:观察不同免疫缺陷小鼠中的人肝癌细胞生长情况以及T,B淋巴细胞的免疫作用,探讨免疫缺陷小鼠肿瘤模型制作的意义。 方法:将体外培养的人肝癌细胞接种到四种免疫缺陷小鼠,分别为:B细胞缺陷的CBA/N,T细胞缺陷的Balb/c-nu,T,B细胞缺陷的SCID及免疫重建的SCID小鼠,观察其生长特点;作鼠脾细胞毒试验,测定外周血CD4^+,CD8^+数分数和荷瘤鼠血清Ig含量的变化;作肝癌细胞凝集试验。 结果:CBA/N和用BALB/c鼠外周血淋巴细胞重建的SCID(B-PBL-SCID)鼠不成瘤,nude、SCID和用CBA/N鼠外周血淋巴细重建的SCID(C-PBL-SCID)小鼠全部成瘤;SCID鼠的瘤体比裸鼠瘤体长的更快,肝内接种转移率更高、转移范围更大。接种瘤细胞的BALB/c和CBA/N鼠脾细胞对癌细胞杀伤力较强,免疫重建的SCID鼠脾细胞毒杀伤较小。接种瘤细胞的鼠CD4^+数分数都下降,CD8^+变化不大,CD4^+/CD8^+比值下降。具有B细胞的实验鼠均测得Ig在mg·L^(-1)水平,并能使癌细胞产生凝集反应。 结论:SCID鼠是建立肿瘤模型和免疫重建研究的理想小鼠;小鼠成瘤率与T细胞相关,T细胞在异种瘤移植排斥中起主要作用;B淋巴细胞及其产生的抗体在抗肿瘤中起着不可忽视的作用。 | 张志培 赵佐庆 张盈华 黄立军 施新猷 | 2002 | 世界华人消化杂志2002，10，4： | 3 |
| 14 | 以肝星状细胞为靶标探讨牛磺酸对门脉高压的影响显示文摘目的以肝星状细胞(HSC)为靶标探讨牛磺酸对门脉高压的影响。方法胶原晶格收缩法观察其对HSC-T6细胞收缩的影响,硝酸还原酶法测定一氧化氮(NO),化学比色法测定一氧化氮合酶(NOS)活性。结果牛磺酸能显著抑制HSC-T6收缩(P<0·05或P<0·01),抑制作用与剂量呈依赖关系;牛磺酸能增加HSC分泌NO和增强NOS的活性。结论牛磺酸具有一定的降低门脉高压的作用。 | 梁健 邓鑫 吴金玉 杨光业 黄仁彬 | 2006 | 广西医学2006，28，2： | 3 |
| 15 | AgNORs计数DNA含量及PCNA与肝硬化增生结节和肝癌的关系显示文摘目的:探讨肝硬化(LC)、增生结节与肝细胞癌(HCC)之间的关系. 方法:分别应用银染色技术、图像分析技术及免疫组织化学技术检测LC、增生结节及HCC中AgNORs计数、DNA 含量及增生细胞核抗原(PCNA)的表达. 结果:增生结节中,其AgNORs计数、DNA含量及.PCNA 的表达均与正常肝组织和LC组织有明显差异(P分别<0.01, 0.05,0.05);其中AgNORs计数与I级HCC相近(P>0.05), DNA含量与HCC相近(P>0.05).LC组织和正常肝组织间的AgNORs计数、DNA含量及PCNA的表达差异均无显著性(P均>0.05). 结论:增生结节与LC是两种不同性质的细胞群体,前者属于活跃增生性病变,是HCC的癌前期病变,后者仍为成熟的细胞,与HCC的发生没有直接关系. | 牛兆山 张昭成 | 2004 | 世界华人消化杂志2004，12，3： | 2 |
| 16 | 缬沙坦治疗肝硬化门静脉高压症患者的临床观察显示文摘目的探讨缬沙坦对肝硬化患者血流动力学及降钙素基因相关肽(CGRP)的影响。方法肝硬化患者分为对照组和治疗组,各18例,对照组给予常规治疗,治疗组在此基础上加用缬沙坦80mg/d口服,疗程1个月。治疗前后应用彩色多普勒超声仪检测门、脾静脉的内径与流速并计算血流量,同时采用放免法检测血浆CGRP的水平。结果治疗组患者门、脾静脉的内径减小,流速增快,门静脉血流量增加,血CGRP水平明显下降,而对照组上述指标的变化均无统计学意义。结论缬沙坦能够通过降低血CGRP水平的途径降低门静脉压力。 | 刘莹 霍丽娟 张锁娟 | 2009 | 山西医科大学学报2009，40，2： | 2 |
| 17 | 实验性肝癌形成过程C-myc IGF-Ⅱ基因与CyclinD1的表达显示文摘目的观察DEN诱发大鼠肝癌过程中C-myc、胰岛素样生长因子Ⅱ(IGF-Ⅱ)基因与细胞周期蛋白cyclinD1的表达变化及其相互关系。方法用原位杂交技术及免疫组化方法检测了大鼠肝癌形成过程中C-myc、IGF-Ⅱ mRNA及cyclinD1的表达变化,计算机图象分析检测结果,并进行统计学相关性分析。结果 C-myc、IGF-Ⅱ基因及cyclinD1在诱癌早期即可检出较多阳性信号,并随着大鼠肝癌诱发的进程逐渐增加,三者表达变化相关性分析具有显著意义(c-myc与IGF-Ⅱ基因阳性信号呈正相关(r=0.8125,P<0.001)。两基因与cyclinD1表达变化呈正相关(r=0.6607,P<0.001、r=0.6071,P<0.001)。结论 C-myc、IGF-Ⅱ基因过表达是肝癌发生的早期事件,并对癌细胞转化表型的维持具有一定意义。两基因的激活可能导致cyclinD1的过表达,三者的协同作用对肝细胞过度增殖以至癌变具有重要意义。 | 凌昌全 钱妍 赵江安 金岩 | 2001 | 世界华人消化杂志2001，9，12： | 2 |
| 18 | IGF2基因印迹丢失与消化道肿瘤的关系显示文摘 | 覃江 | 2007 | 广西医学2007，29，6： | 1 |
| 19 | SNRPN基因在HepG2细胞中的印迹状态研究显示文摘目的:研究小核核糖核蛋白多肽N基因(SNRPN)在肝癌肿瘤HepG2细胞株的表达及基因印迹状态.方法:采用RT-PCR方法检测出SNRPN基因在肝癌肿瘤HepG2细胞株中的表达状况,对HepG2细胞株基因组DNA和cDNA中的SNRPN基因外显子4nt1654312位点用RT-PCR为基础的RFLP方法进行基因分型.结果:HepG2细胞稳定表达SNRPN,SNRPN外显子4nt1654312(数字依据NT_026446,SNPrs705)为杂合子(C/T);RT-PCR为基础的RFLP分析表明,SNRPN的双等位基因中只有T等位基因产生mRNA转录本.结论:SNRPN基因在HepG2肝癌细胞株中有表达,其基因印迹状态未丢失. | 晏泽辉 邓国宏 王宇明 | 2005 | 世界华人消化杂志2005，13，21： | 1 |
| 20 | 依贝沙坦治疗肝硬化大鼠门脉高压症实验研究显示文摘目的:探讨依贝沙坦对大鼠门脉高压症的治疗作用及副作用。方法:成年雄性Wistar大鼠门脉高压形成后,随机分为4组,即模型组(n=7)、依贝沙坦组(n=6)、普萘洛尔组(n=6),另取8只正常大鼠作为正常对照组。治疗14 d,分别从肝静脉、颈动脉插管测量肝静脉压力梯度(HVPG)、平均动脉压(MAP)。用放免法测定血清透明质酸(HA)、型前胶原(PC)含量。结果:与模型组相比,依贝沙坦组和普萘洛尔组的HVPG显著下降(P<0.05),两组间差别无统计学意义,依贝沙坦组的MAP显著下降(P<0.05)。与模型组相比,依贝沙坦组血清HA、PC显著下降(P<0.05)。结论:依贝沙坦具有一定降低大鼠肝硬化门脉压力的作用,但是该药物对全身血流动力学有较大的影响,可引起全身动脉压力的显著下降。 | 王青山 韩晓栋 | 2008 | 临床医药实践2008，17，9： | 0 |
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