|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | HAP纳米粒子进入癌细胞的体外实验研究显示文摘目的验证羟基磷灰石(HAP)纳米粒子与癌细胞共同孵育后,细胞质内发现的大量颗粒物质,是HAP纳米粒子,以证实HAP纳米粒子能以纳米粒子形式进入癌细胞。为进一步深入探讨HAP纳米粒子在癌细胞内的代谢过程提供实验依据。方法将BEL7402肝癌细胞与HAP纳米粒子共同孵育8h后,应用透射电子显微镜观察,并进行了能谱检测和电子衍射图谱分析。结果透射电镜观察到癌细胞内外均有片状的颗粒物质;能谱显示这些颗粒状物质为含Ca、P的粒子;电子衍射图谱测试表明其d值符合HAP晶体的特征。结论进入到肝癌细胞内的颗粒物质即为HAP纳米粒子,说明HAP纳米粒子能以纳米颗粒的形式进入肝癌细胞。 | 扈盛 闫玉华 王友法 曹献英 李世普 | 2005 | 中国生物医学工程学报2005，24，4： | 11 |
| 2 | 纳米活性炭,纳米二氧化硅和纳米二氧化钛对人胃肿瘤BGC-823细胞的毒性作用显示文摘目的探讨不同化学组成的纳米颗粒对人胃癌BGC-823细胞的毒性作用及其机制。方法分别以纳米活性炭(ACNP)、纳米二氧化硅(SiO2)和纳米二氧化钛(TiO2)100,200,400,800和1600mg·L-1悬液作用BGC-823细胞24,48和72h,MTT法检测细胞增殖,比色法检测乳酸脱氢酶(LDH)漏出量。ACNP100mg·L-1,纳米SiO2200mg·L-1,纳米TiO2200mg·L-1作用BGC-823细胞24h,透射电镜观察细胞形态及超微结构的影响。纳米SiO2和纳米TiO2100,200,400mg·L-1作用细胞24h后,AnnexinⅤ-FITC/PI双染法检测细胞凋亡。ACNP、纳米SiO2和纳米TiO2100,200mg·L-1作用细胞48h后,用PI染色法检测细胞周期。结果 ACNP,纳米SiO2和纳米TiO2均能明显抑制BGC-823细胞的增殖,作用72h后的IC50分别为874.2,676.2和883.5mg·L-1。与正常对照组相比,纳米SiO2100~800mg·L-1组LDH漏出量均显著升高,并呈浓度依赖性(r=0.9751,P<0.01),而纳米TiO2100mg·L-1作用细胞24h,LDH漏出量与对照组相比没有显著差异,但随着作用浓度增加和作用时间延长,各组LDH漏出量明显高于对照组(P<0.05)。ACNP100mg·L-1作用24h后,细胞出现细胞质浓缩、细胞核固缩和裂解。纳米SiO2200mg·L-1和纳米TiO2200mg·L-1作用24h后均出现细胞坏死。纳米颗粒ACNP,SiO2和TiO2作用组均可见纳米颗粒进入细胞及线粒体损伤。纳米SiO2100mg·L-1和纳米TiO2100mg·L-1作用24h,细胞坏死率与正常对照组(4.59±1.20)%相比显著升高(P<0.01),分别为(39.40±1.72)%和(14.12±0.90)%(P<0.05);细胞凋亡率与对照组相比没有显著差异。ACNP,纳米SiO2和纳米TiO2100和200mg·L-1作用细胞48h后,S期细胞增多,G0/G1期细胞减少,细胞碎片增多;ACNP组亚二倍体细胞增多。结论 ACNP、纳米SiO2和纳米TiO2能够抑制BGC-823细胞的增殖。ACNP可诱导细胞凋亡。纳米SiO2和纳米TiO2能损伤细胞膜,造成以细胞坏死为主的毒性损伤。 | 曲秋莲 张英鸽 | 2010 | 中国药理学与毒理学杂志2010，24，6： | 5 |
| 3 | 羟基磷灰石纳米粒子负载阿霉素的体外抗肿瘤活性研究显示文摘探讨羟基磷灰石(HAP)纳米粒子负载阿霉素后的体外抗肿瘤活性。通过纳米粒子与人肝癌Bel-7402细胞和人正常肝细胞L-02的体外培养,研究阿霉素负载HAP纳米粒子的心肌毒性,以及对肿瘤细胞凋亡的影响。MTT(四甲基偶氮唑蓝盐)法检测表明,阿霉素负载HAP纳米粒子对Bel-7402细胞的抑制率为95.22%,明显高于HAP纳米粒子(16.26%)和阿霉素(83.36%)(P<0.05)。倒置相差显微镜观察显示,阿霉素负载HAP纳米粒子比单用阿霉素有更大的肿瘤抑制效应,并降低心肌毒性。 | 刘静霆 韩颖超 李世普 马雄华 | 2008 | 中国生物医学工程学报2008，27，4： | 4 |
| 4 | 无机纳米粒子进入后肝癌细胞超微结构的变化显示文摘将生物性状较为稳定的二氧化钛(TiO2)和生物相容性较好的羟基磷灰石(HAP)纳米粒子,与肝癌细胞共同孵育1 h和8 h后,收集细胞并制样,用透射电镜观察肝癌细胞超微结构的变化。结果发现肝癌细胞质内有纳米粒子的团块,周围的细胞质有自行溶解现象,内质网过度肿胀,线粒体肿胀崩解、嵴结构紊乱,核膜周间隙扩大、核固缩等死亡现象。 | 扈盛 李世普 闫玉华 王友法 曹献英 | 2005 | 武汉理工大学学报2005，27，11： | 3 |
| 5 | Apoptosis of Cancer Cells Induced by HAP Nanoparticles显示文摘To confirm apoptosis is one of the hepatoma cells death pathways after HAP nanoparticles absorption, hepatoma cells were collected for ultrathin sections preparation and examined under a transmission electron microscope (TEM) after 1 h incubation with HAP nanoparticle. Apoptosis was detected by TUNEL technique. After absorption, some vacuoles with membrane containing HAP nanoparticles were found in cytoplasma. The nuclear envelope shrinked, and some area pullulated from nucleus. The karyotin became pycnosis and assembled at the edge. An apoptosis body was found. And the data of IOD and numbers of the positive apoptosic signals in nuclear area of slides could illustrate much more apoptosis in the HAP group than those in the control group ( P< 0.001). The experimental results indicate that the HAP nanoparticles can induce cancer cells apoptosis. | 扈盛 | 2005 | Journal of Wuhan University of Technology(Materials Science)2005，20，4： | 2 |
| 6 | 羟基磷灰石纳米粒子抑制结肠癌SW-480细胞生长的体外实验显示文摘目的:本研究旨在明确羟基磷灰石纳米粒子(HAP)在体外能否诱导人结肠癌SW-480细胞凋亡并从凋亡角度探讨其抑癌机制。方法:用HAP以不同浓度作用于人结肠癌SW-480细胞,以诱导其凋亡。用MTT比色法观察其细胞毒性,荧光显微镜、透射电镜、琼脂糖凝胶电泳法及流式细胞术(FCM)等方法来检测凋亡,观察其形态学和生化方面的变化。结果:HAP以剂量和时间依赖的方式抑制人结肠癌SW-480细胞的生长。25 mg/L,50mg/L HAP作用72 h细胞的生长抑制率分别是49.71%和61.03%。12.5-100 mg/L的HAP处理48 h后,结肠癌细胞表现为细胞皱缩、核质浓缩、核碎裂、细胞起泡以及凋亡小体形式等凋亡特征的形态学改变。流式细胞仪均能检测到凋亡峰,12.5,25,50,100 mg/L HAP作用48 h,凋亡率分别是3.57%,21.45%,37.10%和49.45%。结论:HAP在体外能抑制人结肠癌SW-480细胞增殖并诱导细胞凋亡。 | 王雄飞 刘志苏 钱群 唐胜利 | 2006 | 武汉大学学报(医学版)2006，27，3： | 2 |
| 7 | 羟基磷灰石纳米粒子对肝癌H22小鼠肿瘤细胞凋亡的研究显示文摘目的通过不同浓度的羟基磷灰石(HAP)纳米粒子作用于肝癌H22小鼠肿瘤细胞,研究其对肿瘤细胞凋亡的影响。方法100只肝癌H22小鼠随即分组并连续服用不同剂量的HAP纳米粒子,11d后处死小鼠,计算抑瘤率和细胞凋亡率,评价不同剂量的HAP纳米粒子与H22小鼠肿瘤细胞生长的关系。结果肝癌H22小鼠肿瘤细胞对纳米羟基磷灰石(HAP)较敏感,能明显延长小鼠生存期,高剂量组与丝裂霉素结果相近(P>0.05)。实验后小鼠体重没有明显下降,抑瘤率和细胞的凋亡率随浓度升高而增大,呈明显的剂量依赖性(P<0.01)。结论不同浓度的羟基磷灰石(HAP)纳米粒子能够抑制H22肿瘤细胞的增殖,诱导肿瘤细胞的凋亡,并呈剂量依赖性。 | 王培军 罗佳滨 吕冬霞 | 2007 | 解剖学研究2007，29，3： | 2 |
| 8 | Investigation of HAP Nanoparticles Absorbed by Hepatoma Cells in vitro显示文摘Many particles are found in the cytoplasm area after the mixture of hydroxyapatite(HAP) nanoparticles and cultured cancer cells.The purpose of this study was to confirm whether these particles in cytoplasm are HAP nanoparticles exactly.BEL7402 cells were incubated in HAP sol for 8 hours.Then,the cells were collected for specimen preparation.Transmission electron microscope(TEM),energy dispersing spectrum (EDS)and electronic diffraction(ED)attached to TEM were used to detect the properties of the particles.It is found that many particles similar to HAP in shape are in the cytoplasm under TEM.By EDS analysis,they are the particles containing calcium(Ca)and phosphorus(P).The classic rings of HAP crystal appear in the ED pictures of these particles.So the particles are confirmed as HAP nanoparticles.Thus,it is concluded that HAP nanoparticles as the crystal particles can be absorbed by hepatoma cells. | 李世普 | 2007 | Journal of Wuhan University of Technology(Materials Science)2007，22，2： | 0 |
| 9 | Comparative Study on Lattice Parameters of HAP Nanoparticles with Those of HAP Whiskers显示文摘Hydroxyapatite whiskers are used as reinforcement for biomaterials because of their needle- like morphology and strong strength of single crystal.HAP nanoparitcles are used in drug delivery system, protein separation and anticancer drug besides their implant applications.Many new properties arise from nanoeffects while nanoeffects come from specialty of nanostructure.The Ca(NO 3 ) 2 ·4H 2 O and(NH 4 ) 2 HPO 4 were used as starting reagents for preparation of HAP whiskers and nanoparticles.The whiskers are 100-200μm long while nanoparticles are 30-80 nm in size.XRD is applied to characterize the lattice parameter difference of whisker and nanoparticle.Compared with those of HAP whiskers,the a-axis and b-axis of HAP nanocrystals are shortened while the c-axis is elongated.That makes nanoparticles capable of their non-needle-like morphology, higher reaction activity and special | 王友法 | 2008 | Journal of Wuhan University of Technology(Materials Science)2008，23，3： | 0 |
| 10 | Change in Shape and Crystal Structure of HAP Nanoparticles during Absorption into Cell显示文摘The change of hydroxyapatite (HAP) nanoparticles in shape and crystal structure after endocytosis into cancer cells was studied. BEL7402 cells were incubated with HAP nanoparticles for 2 hour,8 hours,20 hours,respectively. Then,the cells were collected and viewed under a transmission electronic microscope (TEM). Electronic diffraction (ED) attached to TEM was used to detect the properties of the particles. The results show that HAP particles in the cytoplasm can be degraded in cytoplasm. The degradation process is prolonged by more than 20 hours. Thus,it is concluded that HAP nanoparticles would be degraded after kill cells or delivery gene. | 扈盛 李世普 | 2007 | Journal of Wuhan University of Technology(Materials Science)2007，22，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员