维普期刊资源整合服务平台（V6.5）

维普中文期刊产品整合服务

共被期刊论文引用了1次 您的检索式：您选中1篇文献正在查看引证文献汇总

选中清除导出查看参考文献查看引证文献引用追踪共1页首页上一页第1页下一页末页 /1 跳转

		题名	作者	年代	出处	被引量
	1	Use of lectin microarray to differentiate gastric cancer from gastric ulcer显示文摘AIM:To investigate the feasibility of lectin microarray for differentiating gastric cancer from gastric ulcer.METHODS:Twenty cases of human gastric cancer tissue and 20 cases of human gastric ulcer tissue were collected and processed.Protein was extracted from the frozen tissues and stored.The lectins were dissolved in buffer,and the sugar-binding specificities of lectins and the layout of the lectin microarray were summarized.The median of the effective data points for each lectin was globally normalized to the sum of medians of all effective data points for each lectin in one block.Formalin-fixed paraffin-embedded gastric cancer tissues and their corresponding gastric ulcer tissues were subjected to Ag retrieval.Biotinylated lectin was used as the primary antibody and HRP-streptavidin as the secondary antibody.The glycopatterns of glycoprotein in gastric cancer and gastric ulcer specimens were determined by lectin microarray,and then validated by lectin histochemistry.Data are presented as mean±SD for the indicated number of independent experiments.RESULTS:The glycosylation level of gastric cancer was significantly higher than that in ulcer.In gastric cancer,most of the lectin binders showed positive signals and the intensity of the signals was stronger,whereas the opposite was the case for ulcers.Significant differences in the pathological score of the two lectins were apparent between ulcer and gastric cancer tissues using the same lectin.For MPL and VVA,all types of gastric cancer detected showed stronger staining and a higher positive rate in comparison with ulcer,especially in the case of signet ring cell carcinoma and intra-mucosal carcinoma.GalNAc bound to MPL showed a significant increase.A statistically significant association between MPL and gastric cancer was observed.As with MPL,there were significant differences in VVA staining between gastric cancer and ulcer.CONCLUSION:Lectin microarray can differentiate the different glycopatterns in gastric cancer and gastric ulcer,and the lectins MPL and VVA can be used as biomarkers.	Wei-Li Huang Yang-Guang Li Yong-Chen Lv Xiao-Hui Guan Hui-Fan Ji Bao-Rong Chi	2014	World Journal of Gastroenterology2014，20，18：	2

返回顶部每页显示：

共1页首页上一页第1页下一页末页 /1 跳转

goolge学术搜索-维普

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费