|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cytological analysis and genetic control of rice anther development显示文摘Microsporogenesis and male gametogenesis are essential for the alternating life cycle of flowering plants between diploid sporophyte and haploid gametophyte generations.Rice(Oryza saliva) is the world's major staple food,and manipulation of pollen fertility is particularly important for the demands to increase rice grain yield.Towards a better understanding of the mechanisms controlling rice male reproductive development,we describe here the cytological changes of anther development through 14 stages,including cell division,differentiation and degeneration of somatic tissues consisting of four concentric cell layers surrounding and supporting reproductive cells as they form mature pollen grains through meiosis and mitosis.Furthermore,we compare the morphological difference of anthers and pollen grains in both monocot rice and eudicot Arabidopsis thaliana.Additionally,we describe the key genes identified to date critical for rice anther development and pollen formation. | Dabing Zhang Xue Luo Lu Zhu | 2011 | Journal of Genetics and Genomics2011，38，9： | 56 |
| 2 | 水稻光温敏雄性核不育系的系谱分析显示文摘近年来,我国两系杂交水稻快速发展,截止2010年底,共有427个两系组合通过审定。两系杂交水稻已经奠定了在我国水稻生产中的重要地位,而光温敏核不育系是两系杂交水稻的基础。本文从实用性角度研究了1994年以来通过审定和获得新品种保护权的两系组合所涉及的130个光温敏核不育系,分析了光温敏核不育系的基本来源和其中126个不育系的系谱。以原始光温敏核不育系为起点演绎了不育系之间的衍生关系,介绍了大面积应用及获得新品种保护权的73个不育系的系谱,归纳了新光温敏核不育系的育成途径。讨论了不育系育性转换的光温反应类型与其核不育基因来源的关系。提出利用光温敏核不育系开展分子生物学研究过程中,有必要通过系谱分析不育系之间的衍生关系,对不育系材料进行有针对性的选择。强调了促进光温敏核不育资源的开放与共享,对加快实用性光温敏核不育系的选育,积极应对两系杂交稻制种环境的变化具有重要意义。 | 斯华敏 付亚萍 刘文真 孙宗修 胡国成 | 2012 | 作物学报2012，38，3： | 36 |
| 3 | A Genetic Pathway for Tapetum Development and Function in Arabidopsis显示文摘In anther development, tapetal cells take part in complex processes, including endomitosis and apoptosis (programmed cell death). The tapetum provides many of the proteins, lipids, polysaccharides and other molecules necessary for pollen development. Several transcription factors, including DYT1, TDF1, AMS, MS188 and MS1, have been reported to be essential for tapetum development and function in Arabidopsis thaliana. Here, we present a detailed cytological analysis of knockout mutants for these genes, along with an in situ RNA hybridization experiment and double mutant analysis showing that these transcription factors form a genetic pathway in tapetum development. DYT1, TDF1 and AMS function in early tapetum development, while MS188 and MS1 are important for late tapetum development. The genetic pathway revealed in this work facilitates further investigation of the function and molecular mechanisms of tapetum development in Arabidopsis. | Jun Zhu Yue Lou Xiaofeng Xu Zhong-Nan Yang | 2011 | Journal of Integrative Plant Biology2011，53，11： | 32 |
| 4 | Maize Genic Male-Sterility Genes and Their Applications in Hybrid Breeding: Progress and Perspectives显示文摘As one of the most important crops, maize not only has been a source of the food, feed, and industrial feedstock for biofuel and bioproducts, but also became a model plant system for addressing fundamental questions in genetics. Male sterility is a very useful trait for hybrid vigor utilization and hybrid seed production. The identification and characterization of genic male-sterility (GMS) genes in maize and other plants have deepened our understanding of the molecular mechanisms controlling anther and pollen development, and enabled the development and efficient use of many biotechnology-based male-sterility (BMS) systems for crop hybrid breeding. In this review, we summarize main advances on the identification and characterization of GMS genes in maize, and con struct a putative regulatory network controlling maize anther and pollen development by comparative genomic analysis of GMS genes in maize, Arabidopsis, and rice. Furthermore, we discuss and appraise the features of more than a dozen BMS systems for propagating male-sterile lines and producing hybrid seeds in maize and other plants. Finally, we provide our perspectives on the studies of GMS genes and the development of novel BMS systems in maize and other plants. The continuous exploration of GMS genes and BMS systems will enhance our understanding of molecular regulatory networks controlling male fertility and greatly facilitate hybrid vigor utilization in breeding and field production of maize and other crops. | Xiangyuan Wan Suowei Wu Ziwen Li Zhenying Dong Xueli An Biao Ma Youhui Tian Jinping Li | 2019 | Molecular Plant2019，12，3： | 24 |
| 5 | Molecular Control of Male Reproductive Development and Pollen Fertility in Rice显示文摘Anther development and male fertility are essential biological processes for flowering plants and are important for crop seed production.Genetic manipulation of male fertility/sterility is critical for crop hybrid breeding.Rice (Oryza sativa L.) male sterility phenotypes,including genic male sterility,hybrid male sterility,and cytoplasmic male sterility,are generally caused by mutations of fertility-related genes,by incompatible interactions between divergent allelic or non-allelic genes,or by genetic incompatibilities between cytoplasmic and nuclear genomes.Here,we review the recent advances in the molecular basis of anther development and male fertility-sterility conversion in specific genetic backgrounds,and the interactions with certain environmental factors.The highlighted findings in this review have significant implications in both basic studies and rice genetic improvement. | Jing-Xin Guo Yao-Guang Liu | 2012 | Journal of Integrative Plant Biology2012，54，12： | 18 |
| 6 | The Genetic Architecture of Flowering Time and Photoperiod Sensitivity in Maize as Revealed by QTL Review and Meta Analysis显示文摘The control of flowering is not only important for reproduction,but also plays a key role in the processes of domestication and adaptation.To reveal the genetic architecture for flowering time and photoperiod sensitivity,a comprehensive evaluation of the relevant literature was performed and followed by meta analysis.A total of 25 synthetic consensus quantitative trait loci(QTL)and four hot-spot genomic regions were identified for photoperiod sensitivity including 11 genes related to photoperiod response or flower morphogenesis and development.Besides,a comparative analysis of the QTL for flowering time and photoperiod sensitivity highlighted the regions containing shared and unique QTL for the two traits.Candidate genes associated with maize flowering were identified through integrated analysis of the homologous genes for flowering time in plants and the consensus QTL regions for photoperiod sensitivity in maize(Zea mays L.).Our results suggest that the combination of literature review,meta-analysis and homologous blast is an efficient approach to identify new candidate genes and create a global view of the genetic architecture for maize photoperiodic flowering.Sequences of candidate genes can be used to develop molecular markers for various models of marker-assisted selection,such as marker-assisted recurrent selection and genomic selection that can contribute significantly to crop environmental adaptation. | Jie Xu Yaxi Liu Jian Liu Moju Cao Jing Wang Hai Lan Yunbi Xu Yanli Lu Guangtang Pan Tingzhao Rong | 2012 | Journal of Integrative Plant Biology2012，54，6： | 14 |
| 7 | 水稻隐性核雄性不育基因研究进展及育种应用探讨显示文摘水稻隐性核雄性不育材料在杂交育种和水稻生产上都具有十分重要的意义。但是,由于缺乏有效的保持和繁殖技术体系,该类不育材料一直未能在生产上获得充分利用。而现代分子与生物技术的快速发展为这些隐性核雄性不育基因的有效利用提供了机会。综述了目前已克隆的水稻隐性核雄性不育基因的研究进展,并总结了玉米、番茄和拟南芥等材料中存在的隐性核雄性不育基因。我们期望利用这些基因信息和新的生物技术手段(例如定点突变技术),创制更多的水稻不育基因,同时利用新的基因工程手段——种子生产技术(SPT)体系,并结合传统的育种方法,有效地解决水稻隐性核雄性不育系的保持和繁殖难题,开创新的杂交育种途径,拓宽水稻杂种优势的利用空间。 | 马西青 方才臣 邓联武 万向元 | 2012 | 中国水稻科学2012，26，5： | 12 |
| 8 | 植物MYB转录因子在花药发育中的调控作用显示文摘MYB转录因子作为最大的转录因子家族之一,参与植物的生长发育、胁迫反应、产物代谢等过程,在植物花的发育特别是花药发育过程中发挥着重要的调控作用。花药的发育在植物繁殖后代中起关键作用,文中就MYB转录因子在花药绒毡层发育、花药开裂、花粉发育、糖类物质和激素途径等方面对花药发育过程中的调控作用进行总结,以期为植物花药发育调控机制及调控网络的深入研究提供可行的参考。 | 胡若琳 袁超 牛义 汤青林 魏大勇 王志敏 | 2020 | 生物工程学报2020，36，11： | 11 |
| 9 | 苹果赤霉素信号转导因子MdGAMYB的克隆和表达分析显示文摘以‘长富2号’苹果为试验材料,从其短枝顶芽中克隆得到1个赤霉素信号转导因子MdGAMYB,对其进行生物信息学和表达分析。结果表明,MdGAMYB的开放阅读框(ORF)长度为1 656bp,编码551个氨基酸,蛋白质分子量为59.741 kD。生物信息学分析表明MdGAMYB编码的蛋白存在多个糖基化位点和磷酸化位点;序列分析表明,Md GAYMB和其他物种的GAMYB蛋白有很高的相似性,均含有保守的R2R3 DNA结合域和GAMYB家族所特有的Box1,Box2和Box3保守区域;系统进化分析表明,Md GAYMB与梨、梅花、草莓、枣和葡萄等的GAMYB蛋白具有较高的同源性。实时荧光定量PCR分析表明,Md GAYMB具有组织表达特异性,在叶片、花和芽中的表达量较高。外源GA3处理抑制了花芽孕育和翌年成花,抑制MdGAMYB的表达。在易成花品种‘烟富6号’中的表达量高于难成花品种‘长富2号’。 | 樊胜 雷超 辛明志 邵红霞 Muhammad Mobeen Tahir 张东 韩明玉 | 2017 | 园艺学报2017，44，5： | 11 |
| 10 | 水稻雄性不育突变体gamyb5的鉴定与基因定位显示文摘在60 Co-γ射线辐射诱变籼稻中恢8015的突变体库内发现了一个无花粉型雄性不育突变体gamyb5。gamyb5的株型、株高、分蘖数等农艺性状与野生型中恢8015无差异,而其花药细长且呈白色半透明状,花药中无花粉粒。花药半薄切片观察结果表明,gamyb5的小孢子母细胞减数分裂异常,没有形成正常的四分体和小孢子,并且绒毡层异常伸长,细胞程序性死亡延迟。对以gamyb5为母本,与野生型中恢8015和广亲和粳稻品种02428分别配制的杂交组合遗传分析表明,gamyb5突变性状受一个隐性核基因控制。利用gamyb5和02428杂交的F2定位群体,最终将突变基因精细定位于第1染色体长臂的ZF-29和ZF-31两个标记之间,物理距离约16.9kb。对该区域内2个完整的开放阅读框测序分析发现,编码受赤霉素诱导的MYB转录因子基因LOC_Os01g0812000的第2外显子存在8个碱基的缺失,导致翻译提前终止。qRT-PCR检测到影响花药发育的调控因子UDT1、TDR、CYP703A3和CYP704B2的表达量在突变体中比野生型中极显著降低。进一步证明GAMYB在花药减数分裂和绒毡层细胞程序性死亡过程中起关键作用。 | 杨正福 张迎信 孙廉平 张沛沛 轩丹丹 刘嶺 胡霞 李紫荷 占小登 吴玮勋 曹立勇 程式华 | 2016 | 中国水稻科学2016，30，2： | 6 |
| 11 | 水稻雄性发育功能基因的发掘及应用显示文摘水稻雄配子体发育包含花药细胞分化、绒毡层细胞程序性死亡、花粉壁形态建成和受精等连续的发育过程,是生殖发育基础理论研究和水稻育种特别是杂交育种的关键过程。现重点介绍近年来国内外水稻雄性发育分子调控机制的最新研究进展,并概述雄性不育系在杂种优势利用、杂交不亲和等重要农艺性状控制方面的理论基础。 | 王多祥 祝万万 袁政 张大兵 | 2016 | 生命科学2016，28，10： | 6 |
| 12 | 优质水稻黄华占雄性不育突变体的筛选及初步分析显示文摘利用EMS(甲磺酸乙酯)处理优质水稻品种黄华占种子,经过田间筛选和对M1、M2代连续的遗传分析得到317份独立的、能够稳定遗传的、由单隐性核基因控制的雄性不育突变材料。通过对花器官形态观察及花粉染色分析,初步将这些突变体材料分为6类,其中花粉发育突变体在不同的花粉发育时期出现异常。这些突变材料为深入研究水稻雄性不育分子机制及开展分子设计育种提供充足的材料。98个无粉型雄性不育突变体用于雄性不育基因TDR的测序分析,其中3个突变体在3个不同的位置上出现单碱基突变。 | 陈竹锋 卢嘉威 卢启清 王娜 王成旭 谢刚 周向阳 唐晓艳 | 2014 | 广东农业科学2014，41，19： | 6 |
| 13 | A novel CCCH-type zinc finger protein SAW1 activates OsGA20ox3 to regulate gibberellin homeostasis and anther development in rice显示文摘Male sterility is a prerequisite for hybrid seed production.The phytohormone gibberellin(GA)is in-volved in regulating male reproductive development,but the mechanism underlying GA homeostasis in anther development remains less understood.Here,we report the isolation and characterization of a new positive regulator of GA homeostasis,swollen anther wall 1(SAW1),for anther development in rice(Oryza sativa L.).Rice plants carrying the recessive mutant allele saw1 produces abnormal anthers with swollen anther wall and aborted pollen.Clustered regularly interspaced short palindromic repeats(CRISPR)/CRIPSR-associated protein 9-mediated knockout of SAW1 in rice generated similar male sterile plants.SAW1 encodes a novel nucleus-localizing CCCH-tandem zinc finger protein,and this protein could directly bind to the promoter region of the GA synthesis gene OsGA20ox3 to induce its anther-specific expression.In the saw1 anther,the significantly decreased OsGA20ox3 expression resulted in lower bio-active GA content,which in turn caused the lower expression of the GA-inducible anther-regulator gene OsGAMYB.Thus,our results disclose the mechanism of the SAW1-GA20ox3-GAMYB pathway in controlling rice anther development,and provide a new target gene for the rapid generation of male sterile lines by genome editing for hybrid breeding. | Bin Wang Ruiqiu Fang Faming Chen Jingluan Han Yao-Guang Liu Letian Chen Qinlong Zhu | 2020 | Journal of Integrative Plant Biology2020，62，10： | 5 |
| 14 | 水稻“三明”显性核不育基因的定位显示文摘'三明显性核不育水稻'突变体是由福建省三明市农业科学研究所于2001年在杂交组合'SE21S/Basmati370'的F2代群体中发现的。其不育性受1个显性基因控制(将该基因命名为SMS)。经过多代回交,该显性不育基因已导入籼稻品种佳福占的遗传背景中(将该不育材料称为佳不育)。为了定位SMS,文章将佳不育与粳稻品种日本晴杂交,并将F1与佳福占测交,构建了一个作图群体。利用SSR和INDEL标记,通过混合分离分析和连锁分析,将SMS定位于第8号染色体上两个INDEL标记ZM30和ZM9之间,约99 kb的区间内。该结果为克隆SMS奠定了基础。 | 杨泽茂 谢小芳 黄显波 王丰青 童治军 段远霖 兰涛 吴为人 | 2012 | 遗传2012，34，5： | 5 |
| 15 | 玉米中绒毡层发育调控基因ZmUdt1克隆的生物信息学分析显示文摘为了研究玉米中绒毡层发育调控基因Udt1的功能与作用,进行生物信息学分析,通过利用Udt1基因培育雄性不育的玉米新品种,来提高杂交种纯度和种质质量并缩短育种年限。该试验采用已知的水稻OsUdt1基因(AY953870.1)为参考序列,使用同源克隆的方式在玉米基因组中得到同源性最高的基因BT068494.1(GenBank),并利用生物信息学方法预测该基因的各级结构以及生物学功能等,同时使用MEGA 7.0程序软件构建分子系统进化树。ZmUdt1基因位于玉米的第2条染色体上,ZmUdt1蛋白由219个氨基酸构成,蛋白的相对分子量为24.48 ku,等电点为5.35,属于亲水蛋白;主要由α-螺旋与无规则卷曲构成二级结构,其三级结构与水稻OsUdt1蛋白的三级结构基本一致,属于HLH超级家族,没有信号肽以及跨膜结构;亚细胞定位预测该蛋白位于细胞核中的可能性较大;通过预测,该蛋白具有翻译、复制与转录、转录调控信号转导等功能,具有17个磷酸化修饰位点,而是否具有其他功能尚没有确定。系统进化树表明,玉米与水稻、高粱等物种遗传距离最近,且与拟南芥的DYT1蛋白也有一定的遗传相关性。通过预测和比较,ZmUdt1和OsUdt1蛋白在结构和功能上具有极高的相似性。研究结果可为进一步研究ZmUdt1蛋白的功能与作用,以及创新玉米的雄性不育系提供理论依据。 | 高嵩 吕庆雪 何欢 张建新 张志军 宋广树 刘伟 | 2018 | 华北农学报2018，33，1： | 5 |
| 16 | 禾本科植物雄性不育遗传机制与利用显示文摘雄性不育作为生殖生物学的重要性状,在植物界普遍存在,对于作物杂种优势的利用具有重要意义,在作物育种中具有重要地位。禾本科(Gramineae)植物是人类粮食和牲畜饲料的主要来源,是具有重要经济价值的大科。文章综述了禾本科植物雄性不育的类型、遗传机制、雄性不育的相关基因及其应用,总结了这方面的研究进展,重点归纳了雄性不育的相关基因,展现了雄性不育理论研究及应用的广阔前景。 | 高金玉 韩冰 杨晓虹 白音德力格尔 李天亮 杨才 | 2017 | 北方农业学报2017，45，1： | 5 |
| 17 | The GAMYB-like gene SlMYB33 mediates flowering and pollen development in tomato显示文摘GAMYBs are positive GA signaling factors that exhibit essential functions in reproductive development,particularly in anther and pollen development.However,there is no direct evidence of the regulation of any GAMYB in these biological processes in tomato(Solanum lycopersicum).Here,we identified a tomato GAMYB-like gene,SlMYB33,and characterized its specific roles.SlMYB33 is predominately expressed in the stamens and pistils.During flower development,high mRNA abundance of SlMYB33 is detected in both male and female organs,such as microspore mother cells,anthers,pollen grains,and ovules.Silencing of SlMYB33 leads to delayed flowering,aberrant pollen viability,and poor fertility in tomato.Histological analyses indicate that SlMYB33 exerts its function in pollen development in the mature stage.Further transcriptomic analyses imply that the knockdown of SlMYB33 significantly inhibits the expression of genes related to flowering in shoot apices,and alters the transcription of genes controlling sugar metabolism in anthers.Taken together,our study suggests that SlMYB33 regulates tomato flowering and pollen maturity,probably by modulating the expression of genes responsible for flowering and sugar metabolism,respectively. | Yan Zhang Bo Zhang Tongwen Yang Jie Zhang Bin Liu Xiangqiang Zhan Yan Liang | 2020 | Horticulture Research2020，7，1： | 5 |
| 18 | 水稻花粉育性相关基因研究进展显示文摘花粉发育为水稻生殖发育不可或缺的过程之一,其育性高低对水稻育种以及经济产量具有重要意义。大量研究发现,水稻花粉发育是严格受基因表达调控的生物学过程。基于此,本文综述与水稻花粉育性相关的蛋白编码基因和调控性miRNA的研究现状,总结有关基因在调控水稻花粉育性方面的生物学功能及作用机制,并对该领域未来的发展趋势作出分析与展望,从而为水稻分子设计育种提供理论参考。 | 马龙 徐薇 窦玲玲 柯笑楠 刘明月 耿艳飞 黄霞 贾玉芳 刘庆坡 | 2019 | 江苏农业科学2019，47，10： | 4 |
| 19 | 水稻无花粉型核雄性不育突变体whf41的鉴定与基因定位显示文摘【目的】水稻花药角质层和蜡质层是花粉囊发育重要的结构支撑和安全屏障。对花粉囊发育相关基因进行表型分析和遗传定位,为进一步克隆相关基因和分子机制研究提供基因资源和理论基础。【方法】从籼稻中恢8015辐射诱变(~60Co-γ)突变体库中分离鉴定出了一个无花粉型雄性不育突变体whf41。对野生型和突变体不同发育时期的花药进行半薄切片观察;并对其成熟期的花药进行扫描电镜观察。将突变体分别与中恢8015和02428杂交,构建BC_1F_1、F_1株系和BC_1F_2、F_2群体,对该表型进行遗传分析,采用图位克隆的方法精细定位目的基因。【结果】表型分析结果显示,whf41突变体的花药瘦小且呈透明乳白色,花药中不包含花粉粒细胞;半薄切片结果显示,突变体的小孢子无法形成正常的花粉外壁,绒毡层细胞异常膨大而不进行程序性死亡,最终膨胀的绒毡层和花粉细胞碎片逐渐融合并充满药室;扫描电镜结果进一步发现突变体花药内外壁均呈平滑状而缺乏脂类物质,花粉细胞逐渐破碎并降解。遗传分析表明,whf41突变体的无花粉型雄性不育性状受一对隐性核基因控制,我们将该基因精细定位于第3染色体短臂XD-5和XD-11两个标记之间,物理距离45.6 kb,其中包含9个开放阅读框。序列分析显示该区间内细胞色素P450基因LOC_Os03g07250的第4个外显子处存在1个单碱基替换和3个碱基的缺失,导致其翻译序列发生一个氨基酸的替换(天冬氨酸→甲硫氨酸)和一个氨基酸(缬氨酸)的缺失,致使其功能改变进而出现该表型。q RT-PCR检测结果表明,whf41突变体中CYP704B2和一系列花药脂质合成与转运相关基因的表达水平均发生了显著下调。【结论】根据本研究结果可推断,Os WHF41是CYP704B2的新等位基因,相关结果进一步明确CYP704B2在水稻花药脂质合成与花粉壁形成过程中的重要作用。 | 轩丹丹 孙廉平 张沛沛 张迎信 吴玮勋 杨正福 占小登 沈希宏 曹立勇 程式华 | 2017 | 中国水稻科学2017，31，3： | 4 |
| 20 | Selection and Characterization of a Novel Photoperiod-Sensitive Male Sterile Line in Upland Cotton显示文摘高地棉花(Gossypium hirsutum L.) 显示出强壮的杂种优势。然而,杂种优势广泛地没由于混合种子生产的高费用被利用。一根光周期敏感的基因男无菌的线的创造能实质地在高地棉花减少混合种子生产的费用。有带绿色的标记的如此的异种被空间变化在接近地球的轨道发现,它的特点在 4 年在 Anyang 和 Sanya 的选择以后是稳定的,中国。当温度比 21.5 敶高时,这异种与一个 1112.5h 光周期是肥沃的吗? | Jianhui Ma Hengling Wei Ji Liu Meizhen Song Chaoyou Pang Long Wang Wenxiang Zhang Shuli Fan Shuxun Yu | 2013 | Journal of Integrative Plant Biology2013，55，7： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员