|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hydrogen sulfide induced by nitric oxide mediates ethylene-induced stomatal closure of Arabidopsis thaliana显示文摘Pharmacological, laser scanning confocal microscopic (LSCM), real-time PCR and spectrophotographic approaches are used to study the roles of hydrogen sulfide (H2S) and nitric oxide (NO) in signaling transduction of stomatal movement response to ethylene in Arabidopsis thaliana. In the present study, inhibitors of H2S synthesis were found to block ethylene-induced stomatal closure of Arabidopsis. Treatment with ethylene induced H2S generation and increased L-/D-cysteine desulfhydrase (pyridoxal-phosphate-dependent enzyme) activity in leaves. Quantitative PCR analysis showed AtL-CDes and AtD-CDes transcripts were induced by ethylene. It is suggested that ethylene-induced H2S levels and L-/D-cysteine desulfhydrase activity decreased when NO was compromised. The data clearly show that ethylene was able to induce H2S generation and stomatal closure in Atnoa1 plants, but failed in the Atnia1,nia2 mutant. Inhibitors of H2S synthesis had no effect on ethylene-induced NO accumulation and nitrate reductase (NR) activity in guard cells or leaves of Arabidopsis, whereas ethylene was able to induce NO synthesis. Therefore, we conclude that H2S and NO are involved in the signal transduction pathway of ethylene-induced stomatal closure. In Arabidopsis, H2S may represent a novel downstream indicator of NO during ethylene-induced stomatal movement. | LIU Jing HOU LiXia LIU GuoHua LIU Xin WANG XueChen | 2011 | Chinese Science Bulletin2011，56，33： | 23 |
| 2 | Hydrogen Sulfide Regulates Ethylene-induced Stomatal Closure in Arabidopsis thaliana显示文摘Hydrogen sulfide (H2S) is a newly-discovered signaling molecule in plants and has caused increasing attention in recent years, but its function in stomatal movement is unclear. In plants, H2S is synthesized via cysteine degradation catalyzed by D-/L-cysteine desulfhydrase (D-/L-CDes). AtD-/L-CDes::GUS transgenic Arabidopsis thaliana (L.) Heynh. plants were generated and used to investigate gene expression patterns, and results showed that AtD-/L-CDes can be expressed in guard cells. We also determined the subcellular localization of AtD-/L-CDes using transgenic plants of AtD-/L-CDes::GFP, and the results showed that AtD-CDes and AtL-CDes are located in the chloroplast and in the cytoplasm, respectively. The transcript levels of AtD-CDes and AtL-CDes were affected by the chemicals that cause stomatal closure. Among these factors, ACC, a precursor of ethylene, has the most significant effect, which indicates that the H2S generated from D-/L-CDes may play an important role in ethylene-induced stomatal closure. Meanwhile, H2S synthetic inhibitors significantly inhibited ethylene-induced stomatal closure in Arabidopsis. Ethylene treatment caused an increase of H2S production and of AtD-/L-CDes activity in Arabidopsis leaves. AtD-/L-CDes over-expressing plants exhibited enhanced induction of stomatal closure compared to the wild-type after ethylene treatment; however, the effect was not observed in the Atd-cdes and Atl-cdes mutants. In conclusion, our results suggest that the D-/L-CDes-generated H2S is involved in the regulation of ethylene-induced stomatal closure in Arabidopsis thaliana. | Zhihui Hou Lanxiang Wang Jing Liu Lixia Hou Xin Liu | 2013 | Journal of Integrative Plant Biology2013，55，3： | 13 |
| 3 | Hydrogen Sulfide May Function Downstream of Nitric Oxide in Ethylene-Induced Stomatal Closure in Vicia faba L.显示文摘Pharmacological, laser scanning confocal microscopic (LSCM), and spectrophotographic approaches were used to study the roles of hydrogen sulfide (H2S) and nitric oxide (NO) in signaling transduction of stomatal movement in response to ethylene in Vicia faba L. Ethylene treatment resulted in the dose-dependent stomatal closure under light, and this effect was blocked by the inhibitors of H2S biosynthesis in V. faba L. Additionally, ethylene induces H2S generation and increases L-/D-cysteine desulfhydrase (pyridoxalphosphate-dependent enzyme) activity in leaves of V. faba L. Inhibitors of H2S biosynthesis have no effect on the ethylene-induced stomatal closure, NO accumulation, and nitrate reductase (NR) activity in guard cells or leaves of V. faba L. Moreover, the ethylene-induced increase of H2S levels and L-/D- cysteine desulfhydrase activity declined when NO generation was inhibited. Therefore, we conclude that H2S and NO probably are involved in the signal transduction pathway of ethylene-induced stomatal closure. H2S may represent a novel component downstream of NO in the ethylene-induced stomatal movement in V. faba L. | LIU Jing HOU Zhi-hui LIU Guo-hua HOU Li-xia LIU Xin | 2012 | Journal of Integrative Agriculture2012，11，10： | 12 |
| 4 | H_2S位于H_2O_2下游参与乙烯诱导拟南芥气孔关闭过程显示文摘H2O2和H2S是植物体内重要的信号分子,二者均参与乙烯诱导的拟南芥气孔关闭过程。以拟南芥野生型及其突变体为材料研究了H2O2和H2S在乙烯诱导拟南芥气孔关闭过程中的相互关系。结果表明,乙烯能够诱导野生型拟南芥叶片H2S含量及L-/D-半胱氨酸脱巯基酶(L-/D-CDes)活性显著增加,促进气孔关闭,但对H2O2合成突变体AtrbohD、AtrbohF、Atpao2和Atpao4植株叶片无显著作用;乙烯亦可引起H2S合成突变体Atl-cdes和Atd-cdes气孔保卫细胞H2O2水平的显著增加,但对其气孔运动没有显著作用。此外,H2O2清除剂和合成抑制剂均能抑制乙烯诱导的拟南芥叶片H2S含量和L-/D-CDes活性的增加及气孔开度的减小;而H2S清除剂和合成抑制剂虽能抑制乙烯诱导的气孔关闭,却不能改变乙烯对拟南芥叶片气孔保卫细胞H2O2的作用效应。由此表明H2S位于H2O2下游介导乙烯诱导拟南芥气孔关闭过程。 | 侯智慧 车永梅 王兰香 侯丽霞 刘新 | 2012 | 植物生理学报2012，48，12： | 10 |
| 5 | Regulatory Function of Polyamine Oxidase-Generated Hydrogen Peroxide in Ethylene-Induced Stomatal Closure in Arabidopsis thaliana显示文摘Hydrogen peroxide (H2O2 ) is an important signaling molecule in ethylene-induced stomatal closure in Arabidopsis thaliana. Early studies on the sources of H2O2 mainly focused on NADPH oxidases and cell-wall peroxidases. Here, we report the involvement of polyamine oxidases (PAOs) in ethylene-induced H2O2 production in guard cells. In Arabidopsis epidermal peels, application of PAO inhibitors caused the failure of ethylene to induce H2O2 production and stomatal closure. Results of quantitative RT-PCR analysis and pharmacological experiments showed that AtPAO2 and AtPAO4 transcripts and activities of PAOs were both induced by ethylene. In transgenic Arabidopsis plants over-expressing AtPAO2 and AtPAO4, stomatal movement was more sensitive to ethylene treatment and H2O2 production was also significantly induced. The increased H2O2 production in the transgenic lines compared to the wild-type plants suggests that AtPAO2 and AtPAO4 probably are involved in ethylene-induced H2O2 production. Several factors which induce stomatal closure such as dehydration and high salinity all enhanced the expression of AtPAO2 and AtPAO4 to different degrees. Moreover, GFP-AtPAOs fusion protein localized in the nucleus, cytoplasm, and cell wall of the guard cells. Therefore, our results strongly indicated that PAO is a source of H2O2 generation in Arabidopsis guard cells and plays crucial roles in stomatal movement. | HOU Zhi-hui LIU Guo-hua HOU Li-xia WANG Lan-xiang LIU Xin | 2013 | Journal of Integrative Agriculture2013，12，2： | 7 |
| 6 | 外源乙烯对长春花生理水平和生物碱积累的影响显示文摘药用植物长春花中含有100多种萜类吲哚生物碱(TIAs),其中具有抗肿瘤功效的长春碱和长春新碱受到关注。为了研究外源乙烯处理对长春花生长情况、生理状态和萜类吲哚生物碱合成的整体影响,本文以长春花幼苗为实验材料,使用外源乙烯处理后对比了不同生长条件下长春花的生物量积累、根茎伸长、光合参数以及生物碱含量等指标,分析了生物碱合成与其他指标之间的相关性。结果表明,外源乙烯处理使长春花乙稀释放量上升,乙烯信号响应因子erf基因表达量提高。乙烯利抑制长春花幼苗生物量积累、根纵向生长,促进茎秆横向加粗生长,由非气孔因素导致净光合速率(P_n)和气孔导度(G_s)下降。外源乙烯促进异胡豆苷(STR)、长春质碱(CAT)、文多灵(VIN)和长春碱(VINB)积累,并且促进长春碱合成途径中关键酶基因str和CrPRX上调表达。相关性分析结果表明,次生代谢产物的积累、生长指标、光合参数之间存在明显的相关性;长春质碱、文多灵、长春碱与茎秆直径(SD)显著正相关(P<0.05),与生物量(B)、株高(H)、根长(RL)、净光合速率(P_n)呈显著负相关(P<0.05)。本文为研究外源乙烯调控长春花生物碱积累的机制提供理论基础。 | 常博文 刘杰 钟鹏 郭晓瑞 | 2018 | 植物研究2018，38，2： | 4 |
| 7 | 乙烯对UV-B辐射诱导蚕豆气孔关闭的调控效应(英文)显示文摘UV-B辐射作为一种重要的环境信号影响着植物的生长与发育,它能够调控气孔运动和诱导乙烯产生。该试验利用乙烯生物合成抑制剂和乙烯受体抑制剂处理蚕豆叶片表皮条,结合气孔开度分析和乙烯释放量测定,研究乙烯在UV-B辐射调控表皮条气孔运动中的作用。结果发现,将蚕豆叶片表皮条置于0.8 W.m-2的UV-B辐射下1~4 h,乙烯生成和气孔关闭均被显著诱导,且乙烯释放峰先于气孔关闭的起始;乙烯生物合成抑制剂和乙烯受体抑制剂处理均能显著逆转UV-B辐射诱导的气孔关闭;外源乙烯处理也能模拟UV-B辐射的效应诱导可见光下蚕豆表皮条的气孔关闭。可见,乙烯介导了UV-B辐射诱导的蚕豆气孔关闭。 | 高晶晶 王瑞斌 贺军民 | 2011 | 西北植物学报2011，31，4： | 3 |
| 8 | eATP位于H_2S下游参与乙烯诱导的拟南芥气孔关闭过程显示文摘以拟南芥(Arabidopsis thaliana)野生型、H2S合成突变体(Atl-cdes和Atd-cdes)和ABC转运体突变体(Atmrp4、Atmrp5和Atmrp4/5)为材料,探讨乙烯诱导气孔关闭过程中e ATP与H2S之间的关系。结果显示,ABC转运体阻断剂格列本脲(Gli)、P2受体抑制剂磷酸吡哆醛-6-偶氮苯基-2',4'-二硫酸(PPADS)和三磷酸腺苷双磷酸酶(Apyrase)可抑制乙烯诱导的气孔关闭;乙烯可提高拟南芥幼苗叶片e ATP含量及At MRP4和At MRP5相对表达量,但对Atmrp4、Atmrp5和Atmrp4/5突变体幼苗叶片e ATP含量和气孔运动没有显著作用。实验结果表明,e ATP是乙烯诱导拟南芥气孔关闭过程的重要信号分子,At MRP4和At MRP5参与胞内ATP的分泌;H2S清除剂次牛磺酸(HT)能阻遏乙烯诱导的拟南芥幼苗叶片e ATP含量的升高;乙烯对Atl-cdes、Atd-cdes幼苗叶片e ATP含量及At MRP4和At MRP5相对表达量无显著影响。据此推测e ATP位于H2S下游参与乙烯诱导的拟南芥气孔关闭过程。 | 车永梅 张丹丹 侯丽霞 王兰香 刘新 | 2015 | 植物学报2015，50，1： | 1 |
| 9 | 磷脂酰肌醇3-磷酸在乙烯诱导蚕豆保卫细胞H_2O_2和NO产生以及气孔关闭中的作用显示文摘以蚕豆(Vicia faba)叶片下表皮为材料,结合药理学试验、气孔开度分析和激光扫描共聚焦显微镜技术,探讨磷脂酰肌醇3-磷酸(PI3P)在乙烯诱导蚕豆保卫细胞中H2O2和NO产生以及气孔关闭中的作用。结果显示,磷脂酰肌醇3-激酶抑制剂沃曼青霉素(WM)和LY294002(LY)显著抑制乙烯释放剂乙烯利诱导的蚕豆保卫细胞H2O2和NO产生以及气孔关闭,外源H2O2和NO处理能完全逆转WM和LY对乙烯利诱导的气孔关闭的抑制效应。结果暗示,PI3P通过诱导信号分子H2O2和NO的产生参与了乙烯诱导蚕豆气孔关闭的细胞信号转导过程。 | 高媛 樊彩明 胡洁 贺军民 | 2015 | 植物生理学报2015，51，8： | 0 |
| 10 | Ethylene-induced stomatal closure is mediated via MKK1/3–MPK3/6 cascade to EIN2 and EIN3显示文摘Mitogen-activated protein kinases(MPKs)play essential roles in guard cell signaling,but whether MPK cascades participate in guard cell ethylene signaling and interact with hydrogen peroxide(H2O2),nitric oxide(NO),and ethylene-signaling components remain unclear.Here,we report that ethylene activated MPK3 and MPK6 in the leaves of wild-type Arabidopsis thaliana as well as ethylene insensitive2(ein2),ein3,nitrate reductase1(nia1),and nia2 mutants,but this effect was impaired in ethylene response1(etr1),nicotinamide adenine dinucleotide phosphate oxidase AtrbohF,mpk kinase1(mkk1),and mkk3 mutants.By contrast,the constitutive triple response1(ctr1)mutant had constitutively active MPK3 and MPK6.Yeast two-hybrid,bimolecular fluorescence complementation,and pull-down assays indicated that MPK3 and MPK6 physically interacted with MKK1,MKK3,and the C-terminal region of EIN2(EIN2 CEND).mkk1,mkk3,mpk3,and mpk6 mutants had typical levels of ethylene-induced H2O2 generation but impaired ethylene-induced EIN2 CEND cleavage and nuclear translocation,EIN3 protein accumulation,NO production in guard cells,and stomatal closure.These results show that the MKK1/3–MPK3/6 cascade mediates ethylene-induced stomatal closure by functioning downstream of ETR1,CTR1,and H2O2 to interact with EIN2,thereby promoting EIN3 accumulation and EIN3-dependent NO production in guard cells. | Teng-Yue Zhang Zhong-Qi Li Yu-Dong Zhao Wen-Jie Shen Meng-Shu Chen Hai-Quan Gao Xiao-Min Ge Hui-Qin Wang Xue Li Jun-Min He | 2021 | Journal of Integrative Plant Biology2021，63，7： | 0 |
| 11 | PI3P和NO在UV-B诱导蚕豆气孔关闭中的关系显示文摘以蚕豆(Vicia faba L.)叶片下表皮为材料,结合气孔开度分析和保卫细胞内源一氧化氮(NO)水平的测定,研究了NO和磷脂酰肌醇3-激酶(PI3K)的催化产物磷脂酰肌醇3-磷酸(PI3P)在紫外线B(UV-B)诱导气孔关闭中的关系。结果显示:UV-B辐射诱导蚕豆保卫细胞NO产生和气孔关闭的效应能被PI3K抑制剂沃曼青霉素(WM)和LY294002(LY)显著抑制。同时,外源NO释放剂硝普钠(SNP)处理能完全逆转WM和LY对UV-B诱导气孔关闭的抑制效应,而WM和LY却不能抑制外源SNP诱导蚕豆气孔关闭的效应。结果说明,在UV-B诱导蚕豆气孔关闭的信号转导途径中PI3P的作用在NO上游。 | 樊彩明 王静 胡洁 贺军民 | 2015 | 陕西师范大学学报(自然科学版)2015，43，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员