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| 1 | 苏丹红Ⅰ、Ⅲ和Ⅳ对HepG-2细胞和SGC-7901细胞增殖的影响显示文摘研究苏丹红Ⅰ、Ⅲ和Ⅳ对人肝癌细胞HepG-2,胃癌细胞SGC-7901增殖的影响.利用激光共聚焦显微镜及三维图像分析HepG-2细胞受到苏丹红Ⅰ、Ⅲ和Ⅳ不同剂量作用后DNA和RNA含量的比值,流式细胞仪测定SGC-7901细胞DNA含量的变化,分析细胞周期的变化.结果表明,对照组细胞DNA/RNA比值为1.223 2±0.084 4,苏丹红Ⅰ、Ⅲ和Ⅳ处理后的HepG-2细胞DNA荧光染色强度明显高于RNA,以低剂量组的作用显著(p<0.01),其DNA/RNA比值分别为1.609 6±0.199 0,1.445 5±0.163 3,1.708 1±0.109 0.三维图表明,HepG-2细胞DNA荧光多聚集于细胞核区域,较RNA浓集、荧光强度大;可促进SGC-7901细胞由G1期进入S期和G2期,表现为G1期细胞平均DNA含量下降,S期DNA含量上升,G2期DNA含量上升,细胞增殖指数(PI)增加,S期细胞(SPF)指数增加.苏丹红Ⅰ、Ⅲ、Ⅳ可以通过影响细胞周期,从而调节细胞的增殖活动,促进细胞分化增殖. | 季宇彬 汲晨锋 高世勇 郎朗 于蕾 | 2006 | 环境科学2006，27，6： | 20 |
| 2 | Gene expression profiles of hepatoma cell line HLE显示文摘AIM: To investigate the global gene expression of cancer related genes in hepatoma cell line HLE using Atlas Human Cancer Array membranes with 588 well-characterized human genes related with cancer and tumor biology.METHODS: Hybridization of cDNA blotting membrane was performed with 32P-labeled cDNA probes synthesized from RNA isolated from Human hepatoma cell line HLE and noncirrhotic normal liver which was liver transplantation donor.AtlasImage, a software specific to array, was used to analyze the result. The expression pattern of some genes identified by Atlas arrays hybridization was confirmed by reverse transcription polymerase chain reaction (RT-PCR)in 24 pairs of specimens and Northern blot of 4 pairs of specimens.RESULTS: The differential expression of cell cycle/growth regulator in hepatocellular carcinoma (HCC) showed a stronger tendency toward cell proliferation with more than 1.5-fold up-regulation of Cyclin C, ERK5, ERK6, E2F-3, TFDP2 and CK4. The anti-apoptotic factors such as Akt-1 were up-regulated, whereas the promotive genes of apoptosis such as ABL2 were down-regulated. Among oncogene/tumors suppressors, SKY was down-regulated. Some genes such as Integrin beta 8, Integrin beta 7, DNA-PK, CSPCP,byglycan, Tenacin and DNA Topo were up-regulated. A number of genes, including LAR, MEK1, eps15, TDGF1,ARHGDIA were down-regulated. In general, expression of the cancer progression genes was up-regulated, while expression of anti-cancer progression genes was downregulated. These differentially expressed genes tested with RT-PCR were in consistent with cDNA array findings.CONCLUSION: Investigation of these genes in HCC is helpful in disclosing molecular mechanism of pathogenesis and progression of HCC. For the first time few genes were discovered in HCC. Further study is required for the precise relationship between the altered genes and their correlation with the pathogenesis of HCC. | Lian-XinLiu Zhi-HuaLiu Hong-ChiJiang Wei-HuiZhang Shu-YiQi JieHu Xiu-QinWang MinWu | 2003 | World Journal of Gastroenterology2003，9，4： | 10 |
| 3 | 激光共聚焦显微镜在肿瘤研究中的应用显示文摘激光共聚焦显微镜(LSCM)是当今最为先进的光学显微镜,是形态学、分子生物学、神经科学、药理学、遗传学等领域中新一代强有力的研究工具。目前在肿瘤研究领域中的荧光定性、定量分析、细胞内离子分析、活细胞动态监测、三维结构观察等也得到较广泛应用。 | 高晓林 王和审 | 2004 | 国外医学(肿瘤学分册)2004，31，10： | 2 |
| 4 | 激光共聚焦显微重建鲜红斑痣三维结构的初步研究显示文摘目的:探讨利用激光共聚焦显微重建鲜红斑痣三维结构可行性,初步建立利用激光共聚焦显微重建鲜红斑痣三维结构的方法。方法:鲜红斑痣石蜡标本切取30μm厚的切片,用鼠抗人anti-cd34、羊抗鼠FITC标记二抗免疫荧光染色,再用激光共聚焦显微镜观察,重建鲜红斑痣三维立体结构图像。结果:利用激光共聚焦显微完成了对10μm及30μm厚鲜红斑痣组织的三维结构重建,获得了鲜红斑痣三维结构的高清图形。结论:利用激光共聚焦显微镜重建鲜红斑痣三维结构组织图像是可行的,对进一步了解鲜红斑痣的结构及治疗有一定的意义。 | 田克斌 韩丽林 江银华 周国瑜 | 2011 | 温州医学院学报2011，41，5： | 2 |
| 5 | A study of the effect of Sudan I, III, and IV on the DNA/RNA ratio and 3D structure of HepG-2 using LCM显示文摘To explore the effect of sudan I, III, and IV on the DNA/RNA ratio and changes in the 3D structure of HepG-2. LCM and 3D images are used to detect the DNA/RNA ratio and changes in the 3D structure of HepG-2 when treated with different dosages of sudan I, III, and IV. The DNA/RNA ratio of the control group is 1.223 2±0.084 4, while the fluorescence intensity of DNA in HepG-2 treated with sudan I, III, or IV is markedly greater than that of RNA, with the low-dosage group showing significant effect (P<0.01), yielding DNA/RNA ratios of 1.609 6±0.199 0, 1.445 5±0.163 3, 1.708 1±0.109 0 respectively; 3D images show that DNA fluorescence in HepG-2 is mostly concentrated in the nuclear region, and is denser and stronger than RNA fluorescence. The DNA/RNA ratio of a treated group increases after being treated with different dosages of sudan, but it declines with increasing dosage, and within a certain dosage range, sudan I, III, and IV are shown to promote the growth of HepG-2. | 季宇彬 汲晨锋 高世勇 朗郎 | 2006 | Journal of Harbin Institute of Technology(New Series)2006，13，2： | 0 |
| 6 | 利用整体染色与透明技术观察棉花胚囊方法显示文摘目的:探索一种观察棉花胚囊的方法。方法:用整体染色与透明技术对棉花胚囊进行处理,利用激光扫描共聚焦显微技术进行观察。首先在解剖镜下将内外珠被剥掉,取出胚囊;然后水解过程需要反复实验得到合适的时间,否则荧光染料很难进入细胞;最后材料在激光扫描共聚焦显微镜上用488nm激光激发观察并摄像。结果:可以清晰地观察到棉花胚囊组织的各组成部分及其细胞结构。结论:利用激光扫描共聚焦显微镜的重组功能构建胚囊的立体结构,有助于了解胚囊各组成细胞的空间结构,为观察棉花及其他较大子房或胚珠提供了一种简单快捷的方法。 | 李建欣 代西梅 张佳佳 张永山 | 2014 | 实验技术与管理2014，31，11： | 0 |
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