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| 1 | 慢性病毒性肝炎发病机制的分子生物学研究显示文摘由乙型肝炎病毒(HBV)和丙型肝炎病毒(HCV)的感染引起的急性和慢性肝病目前还没有满意的治疗方法.新型治疗方法和治疗药物的研究开发,依赖于肝炎病毒致病的分子生物学机制的研究进展.关于HBV感染个体准种概念的引入,使我们对于HBV基因变异的研究,从单一病毒的基因变异上升到群体病毒的基因变异?从静态的基因突变上升到动态的基因变异,从而对HBV存在状态的看法发生了根本的改变.HBV感染肝细胞的相关受体蛋白虽然进行了多年的研究,但还没有最终确定.利用新型技术对于HBV表面抗原蛋白的结合蛋白进行筛选是一个重要的方向.HBV和HCV感染与肝细胞癌之间的关系已经得到确定,但是具体的分子生物学机制还有许多工作要做.研究这2种肝炎病毒反式激活作用的把基因是阐明其引起肝细胞癌分子生物学机制的重要途径.在肝细胞中表达的肝炎病毒蛋白不是孤立存在的,或者与其自身结合形成同二聚体,或者与病毒的其他蛋白?肝细胞蛋白结合形成异二聚体,从而对于肝细胞的生长?代谢?甚至是恶性转化产生重要影响. | 成军 | 2002 | 世界华人消化杂志2002，10，2： | 141 |
| 2 | 丙型肝炎病毒NS2基因酵母双杂交“饵”载体构建及表达显示文摘 | 李克 王琳 成军 陆荫英 洪源 刘妍 张玲霞 | 2002 | 世界华人消化杂志2002，10，2： | 55 |
| 3 | Methodologic research on TIMP-1,TIMP-2 detection as a new diagnostic index for hepatic fibrosis and its significance显示文摘AIM: To set up a new method to detect tissue inhibitors ofmetalloproteinase1 and -2(TIMP-1 and TIMP-2) in sero ofpatients with hepatic cirrhosis, and to investigate theexpression and location of TIMP-1 and TIMP-2 in liver tissueof patients with hepatic cirrhosis, and the correlationbetween TIMPs in liver and those in sera so as to discusswhether TIMPs can be used ss a diagnosis index of hepaticfibrosisMETHODS: The monoclonal antibodies (McAbs) of TIMP-1and TIMP-2 were used to sensitize erythrocytes, and solid-phase absorption to sensitized erythrocytes (SPASE) wasused to detect TIMP-1 and TIMP-2 in the sera of patients withhepatic cirrhosis. Meanwhile, with the method of in situhybridization and immunohistochemistry, we studied themRNA expression and antigen location of TIMP-1 and TIMP-2in the livers of 40 hepatic cirrhosis patients with pathologicdiagnosis.RESULTS: With SPASE, they were 16.4 % higher in theacute hepatitis group, 33.3 % higher in the chronic hepatitisgroup, and the positive rates were 73.6 % and 61. 2 %respectively in sero of hepatic cirrhosis patients, which wereremarkably higher than those in chronic hepatitis and acutehepatitis group ( P < 0. 001 ). In 40 samples of hepaticcirrhosis tissues, all of them showed positive expression ofTIMP-1 and TIMP-2 mRNA detected withimmunohistochemistry or in situ hybridization (positive ratewas 100 % ). Expression of TIMPs in different degrees couldbe found in liver tissue with cirrhosis. TIMPs were located incytoplasm of liver cells of patients with hepatic cirrhosis.There was a significant correlation between serum TIMPslevel and liver TIMPs level.CONCLUSION: SPASE is a useful method to detect the TIMP-1 and TIMP-2 in sera of patients with hepatic cirrhosis, andTIMP-1 and TIMP-2 can be considered as a useful diagnosticindex of hepatic fibrosis, especially TIMP-1. | Oing-He Nie Yong-Oian Cheng Yu-Mei Xie Yong-Xing Zhou Bai-Xian Guang Yi-Zhan Cao,The Centre of Diagnosis and Treatment for Infectious Disease of Chinese PLA,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,Shanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 51 |
| 4 | Expression of p53 and C-myc genes and its clinical relevance in the hepatocellular carcinomatous and pericarcinomatous tissues显示文摘AIM: To investigate the possible roles of p53 and C-mycgenes in the primary hepatocellular carcinogenesis and therelationship between the liver hyperplastic nodule(LHN) andhepatocellular carcinoma(HCC).METHODS: The expression of p53 and C-myc genes wasdetected immunohist-ochemically in 73 and 60 cases of HCCand pericarcinomatous tissues, respectively .RESULTS: The positive expression of p53 in HCC wassignificantly higher than that in pericarcinomatous tissues(P<0.05). In pericarcinomatous tissues, the p53 expressionwas observed only in LHN, but not in liver cirrhosis (LC) andnormal liver tissues. The positive expression rate of C-mycin HCC or LHN was significantly higher than that in LC ornormal liver tissues (P<0.05 and P<0.01), however, nosignificant difference was found between HCC and LHN(P>0.05). The positive expression rate of p53 and C-myc inHCC was correlated with the histological differentiation, thatin the poorly differentiated was significantly higher than thatin well differentiated samples (P<0.05).CONCLUSION: The overexpression of p53 and C-myc genesmight play a role in the carcinogenesis of HCC; And LHNseems a preneoplastic lesion related to hepatocarcinogenesis;No evidence supports that LC contribute directly to thehepatocarcinogenesis. | Zhao-Shan Niu Bo-Kian Li Department of Pathology,Medical College of Qingdao University,Qingdao 266021,Shandong Province,China Mei Wang Department of Foreign languages,Qingdao institute of Architecture and Engineering,Qingdao 266033,Shandong Province,China | 2002 | World Journal of Gastroenterology2002，8，5： | 30 |
| 5 | 丙型肝炎病毒核心蛋白结合蛋白6基因转染肝癌细胞的基因表达谱芯片分析显示文摘目的:筛选并克隆丙型肝炎病毒(HCV)核心蛋白的肝细胞结合蛋白基因,并对新基因转染肝癌细胞的基因表达谱进行分析,探索该基因表达对肝细胞基因表达的调节机制。方法:应用酵母双杂交技术,以HCV的核心蛋白作为“诱饵(bait)”,筛选鉴定与其结合的肝细胞中蛋白的编码基因。应用生物信息学(bioinformatics)技术,分析其中筛选得到的人HCV核心蛋白结合蛋白6(HCBP6)基因的全长编码序列,并构建HCBP6基因的真核表达载体pcDNA3.1(-)-HCBP6。应用基因表达谱芯片技术对重组表达质粒pcDNA3.1(-)-HCBP6转染的HepG2细胞和空载体处理的相同细胞差异表达的mRNA进行检测。结果:通过酵母双杂交技术的筛选和鉴定,结合生物信息学分析,确定人的HCBP6基因由456 nt组成,编码152 aa的蛋白。基因表达谱芯片所检测的1 152条目的基因均为GenBank中登录的基因,HCBP6表达质粒转染的细胞有20条差异表达基因,其中13条基因表达增强,7条基因表达降低。这些差异表达的基因与细胞信号转导、增生、分化及生长调节密切相关。结论:酵母双杂交技术结合生物信息学技术,是克隆蛋白结合蛋白的有效方法,基因表达谱芯片技术对于初步全面探索新基因的功能提供重要的资料。本实验结果为进一步阐明HCV核心蛋白与Hcbp6相互作用后的肝细胞生物大分子变化提供了理论依据。 | 刘妍 成军 李克 杨倩 陆荫英 王琳 王建军 | 2003 | 世界华人消化杂志2003，11，4： | 29 |
| 6 | mIL-2R,T cell subsets & hepatitis C显示文摘AIM: To study the levels of membrane interleukin-2 receptor(mIL-2R ) and T cell subsets in peripheral bloodmononuclear cells (PBMC) from patients with hepatitis Cand their role in the pathogenesis of hepatitis C.METHODS: The levels of mlL-2R and T cells subsets in PBMCWere detected by biotin- streptstividin (BSA) technique beforeand after stimulation with PHA in 203 patients with hepatitis Cwith HCV-RNA( + ), anti-HCV( + ), anti-HCV(-).RESULTS: The total expressive levels of mlL-2R before andafter stimulation with PHA(0.03 ± 0.01, 0.03 ± 0.02, 0.04 ± 0.02, 0.36±0.03), and Tcell subsets in PBMC (0.62±0.06,0.37 ± 0.05, 0.35 ± 0.07) were all lower in patients withhepatitis C than those in normal controls (0.66 ± 0.07, 0.41± 0.06, 0.31 ± 0.05, P < 0.01 ). Among the patients, thelevels of mlL-2R were lower in silence than those in situationof PHA inducting (P< 0.01). However, the levels of mlL-2Rwere similar in acute hepatitis C to that in chronic hepatitis C(P>0.05). The levels of CD3+, CD4+, CD4 +/CD8+ Were lov erand CD8 + was higher in patients with acute and chronichepatitis C with anti-HCV( + ) than those in normal controls (0.62±0.06, 0.37±0.05, 0.35±0.07, 1.18±0.30, 0.61±0.07, 0.37±0.05, 1.39±0.33, 0.31±0.05, P<0.05-P<0.01).CONCLUSION: The cellular immunity is obviously changed inpatients with hepatitis C. The levels of mlL-2R end activationof T cells am closely associated with chronicity of hepatitis C. | Chao-Pin Li Ke-Xia Wang Jian Wang,Department of Aetiology and Immunology,School of Medicine,Huainan University of Technology,Huainan 232001,Anhui Province,China Bo-Rong Pan,the Fourth Military Medical University | 2002 | World Journal of Gastroenterology2002，8，2： | 24 |
| 7 | Effect of 5-Aza-2'-deoxycytidine on the P16 tumor suppressor gene in hepatocellular carcinoma cell line HepG2显示文摘INTRODUCTIONHepatocellular carcinoma (HCC) is one of the mostcommon human malignancies worldwide[1,2], and isclosely associated with infection of HBV and HCVand contamination of aflatoxin B1[3-6]. Althoughthe molecular mechanisms of hepatocarcinogenesisremain poorly understood, an increasing number ofgenetic abnormalities have been recognized[7-10],for example, the p16 gene[11,12] the p53gene[13-18], the E-cadherin gene[19], and the c-mycgene[20]. | Li Hua Liu1 Wen Hua Xiao2 Wei Wen Liu3 1Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China (now working in Department of Gastroenterology, General Hospital of PLA, Lanzhou 730050, Gansu Province, China)2Department of Oncology3Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China | 2001 | World Journal of Gastroenterology2001，7，1： | 21 |
| 8 | Preparation of human single chain Fv antibody against hepatitis C virus E2 protein and its identification in immunohistochemistry显示文摘AIM: To screen human single chain Fv antibody (scFv)against hepatitis C virus E2 antigen and identify its applicationin immunohistochemistry.METHODS: The phage antibody library was panned by HCVE2 antigen, which was coated in microtiter plate. After fiverounds of biopanning,56 phage clones were identified specificto HCV E2 antigen. The selected scFv clones were digestedby SfiI/NotI and DNA was sequenced. Then it was subclonedinto the vector pCANTABSE for expression as E-taggedsoluble scFv. The liver tissue sections from normal personand patients with chronic hepatitis B and chronic hepatitis Cwere immunostained with HCV E2 scFv antibody.RESULTS: The data of scFv-E2 DNA digestion and DNAsequencing showed that the scFv gene is composed of 750bp. ELISA and immunohistochemistry demonstrated that thehuman single chain Fy antibody against hepatitis C E2 antigenhas a specific binding character with hepatitis virus E2 antigenand paraffin-embedded tissue, but did not react with liver tissuesfrom healthy persons or patients with chronic hepatitis B.CONCLUSION: We have successfully screened andidentified HCV E2 scFv and the scFv could be used in theimmunostaining of liver tissue sections from patients withchronic hepatitis C. | Yan-Wei Zhong Jun Cheng Gang Wang Shuang-Shuang Shi Li Li Ling-Xia Zhang Ju-Mei Chen Gene Therapy Research Center,Institute of Infectious Diseases,302 Hospital of PLA,26 Fengtai Road,Beijing 100039,China | 2002 | World Journal of Gastroenterology2002，8，5： | 21 |
| 9 | Tumor suppressor gene p16 and Rb expression in gastric cardia precancerouslesions from subjects at a high incidence area in northern China显示文摘AIM: To further understand the molecular basis for gastriccardia carcinogenesis and to provide etiological clues.METHODS: Endoscopic mucosa biopsy and histopathologicalexaminations were made on 37 subjects from a high incidencearea for both esophageal and gastric cardia carcinomas innorthem China. All the biopsy samples were fixed in 850 mi. -1 Lalcohol and embedded in paraffin. Each block contained onepiece of tissue and was serially section at 5 μm.Immunohistochemistry (ABC) was carried out on these gastriccardia samples to determine the alterations of p16 and Rb.RESULTS: Based on the histopathlogical examinationtherewere 11 cases of chronic superficial gastritis, 12 cases ofchronic atrophic gastritis and 14 cases of dysplasia. Theimmunostaining demonstrated different levels of unclearimmunostaining of p16 and Rb in normal gastric cardiatissue and the tissues with different severity of lesions. Withthe lesions progressing, the positive immunostaining ratesfor pi6 protein had a decreasing tendency. In contrast, thepositive immunostaining rate for Rb protein had anincreasing tendency. There was a significant negativerelationship between the two parameters. Changes of p16wasCSG 11(100 % ), CAG 7(58 % ), DYS 4(29 % ) andchanges of Rb was CSG 2(18 %), CAG 8(67 %) and DYS 12(86 %), (p<0.05).CONCLUSION: The alterations of p16 and Rb protein may playa role in the early stages of gastric cardia carcinogenesis. | ZhouY GaoSS 等 | 2002 | World Journal of Gastroenterology2002，8，3： | 18 |
| 10 | 丙型肝炎病毒包膜蛋白E2抗独特型人源单链可变区抗体的筛选与鉴定显示文摘目的:制备抗丙型肝炎病毒(HCV)包膜蛋白E2(E2)的抗独特型单链可变区抗体scFv(抗-IdscFv),为研制HCVE2的抗-IdscFv疫苗奠定基础.方法:采用噬菌体表面展示技术,将抗HCVE2单克隆抗体固相包被于Nunc板,从噬菌体单链可变区抗体库中经过5轮“黏附-洗脱-扩增”筛选过程,随机挑选出53个克隆,利用酶联免疫黏附法、交叉反应和竞争抑制实验,对其进行免疫学检测,获得与HCVE2单克隆抗体结合活性较强的抗独特型抗体单链可变区片段(抗-IdscFv)的阳性克隆,并对HCVE2特异性抗-IdscFv的编码序列进行序列测定分析.结果:对噬菌体单链可变区抗体库经过5轮“黏附-洗脱-扩增”的筛选后,结合到包被平皿的噬菌体与第一轮相比,富集了12倍.用酶联免疫黏附实验(ELISA)方法测定第五轮筛选后上清液中含有的抗-IdscFv与HCVE2单克隆抗体结合活性.其中有18株克隆ELISA的吸光度(A450nm)值较高(E11A450nm0.928,E14A450nm1.152,E17A450nm1.136,E28A450nm1.163,E53A450nm0.965).对这些噬菌体抗体进行与牛血清白蛋白(BSA)的交叉反应后,确定其中有5株交叉反应较弱(E11A450nm0.044,E14A450nm0.062,E17A450nm0.166,E28A450nm0.012,E53A450nm0.069),结合2次ELISA重复实验的A值及竞争抑制实验结果,最后确定1株(E28)阳性克隆.提取质粒,进行DNA序列测定,DNA大小为768bp.结论:用噬菌体抗体库技术能够成功地获得单抗HCVE2的抗-IdscFv,本实验结果为开展用抗-IdscFv防治丙型肝炎的研究创造了条件. | 钟彦伟 成军 蔡炯 王刚 洪源 王琳 李莉 张玲霞 陈菊梅 | 2002 | 世界华人消化杂志2002，10，8： | 12 |
| 11 | 西安酒精性肝病流行病学显示文摘目的:酒精性肝病近年呈增长之势,世界各国对其越来越重视,本研究采用流行病学方法调查西安地区人群的饮酒情况,初步明确酒精性肝病的患病率及与饮酒的关系。方法:将西安城乡9种代表性职业人群作为本次调查对象,整群随机抽样,采取问卷调查方法,由专人统一询问检测结果:2000-04/06共调查9种职业人群3613例,其中饮酒人数1270例,占35.2%,90.1%为男性,是男性群体的52.2%,女性仅126例,检出酒精性肝病患者82例,占6.5%,为调查人数的2.3%,只1例女性,酒精性肝硬化4例,分别占0.3%和0.1%,78例酒精性脂肪肝患者日均饮酒31.3±24.2g,平均饮酒14.0±7.4a,且随饮酒量和饮酒时限增加,ALT和AST亦渐升高,发病高峰年龄在40岁左右,营养不良可能加重肝功能损伤。结论:西安地区饮酒现象较普遍,男性为主要饮酒人群,酒精性脂肪肝为酒精性肝病最常见类型,患病率2.2%,日均饮酒30g,持续14a即可能患此病,且与ALT、AST水平有正向关系。 | 鲁晓岚 陶明 罗金燕 耿燕 赵平 赵红莉 | 2003 | 世界华人消化杂志2003，11，6： | 12 |
| 12 | Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction显示文摘AIM To study persistence and replication ofheltitis C virus (HCV) in patients' peripheralblood mononuclear cells (PBMC) cultured invitro.METHODS Epstein-Barr virus (EBV) was usedto transform the hepatitis C virus from a HCVpositive patient to permanent lymphoblastoidcell lines (LCL). Positive and negative HCV RNAstrands of the cultured cells and growth mediawere detected by reverse transcriptase-polymerase chain reaction ( RT-PCR ) eachmonth. Core and NS5 proteins of HCV werefurther tested using immunohistochemical SPmethod and in situ RT-PCR.RESULTS HCV RNA positive strands wereconsistently detected the cultured cells for oneyear. The negative-strand RNA in LCL cells andthe positive-strand RNA in supernatants wereobserved intermittently. Immunohistochemicalresults medicated expression of HCV NS3 and Cproteins in LCL cytoplasm mostly. The positivesignal of PCR product was dark blue and mainlylocalized to the LCL cytoplasm. The RT-PCRsignal was eliminated by overnight RNasedigestion but not DNase digestion.CONCLUSION HCV may exist and remainfunctional in a cultured cell line for a longperiod. | Ji Lin Cheng Bao Ling Liu Yi Zhang Wen Bin Tong Zheng Yan Bai Fang Feng Institute of Hepatology,Peoples Hospital,Medical Center of Beijing University,Beijing 10(X)44,China | 2001 | World Journal of Gastroenterology2001，7，3： | 11 |
| 13 | Prevalence of hepatitis B and C markers among refugees in Athens显示文摘AIM: To assess the prevalence of hepatitis B and C serological markers in a population of refugees living in Athens.METHODS: One hundred and thirty refugees (81 males and 49 females, mean age ±SD: 31.7±8 years) were included in the study. The hepatitis B virus surface antigen (HBsAg),the hepatitis B virus core antibody (anti-HBc) and the hepatitis C virus antibody (anti-HCV) were detected using a third-generation immunoassay.RESULTS: Twenty individuals (15.4%) were HBsAg positive and 69 (53.1%) were anti-HBc positive. The prevalence of HBsAg and anti-HBc was higher among refugees from Albania and Asia (statistical significant difference, P<0.008 and P<0.001 respectively). The prevalence of these markers was found irrelevant to age or sex. Anti-HCV was detected in the serum of 3 individuals (2.3 %). No differences among age, sex or ethnicity regarding anti-HCV prevalence were found.CONCLUSION: It can be concluded that refugees living in Athens are an immigrant population characterized by a high incidence of HBV infection. The prevalence of HBV markers is higher among refugees from Albania and Asia. It is therefore believed that the adherence to general precautions and the initiation of HBV vaccination programs will be necessary in the future, especially in these communities.Although the prevalence of HCV infection seems to be relatively low, extended epidemiological surveys are needed to provide valid results. | Anastasios Roussos Constantin Goritsas Thomas Pappas Maria Spanaki Panagiota Papadaki Angeliki Ferti | 2003 | World Journal of Gastroenterology2003，9，5： | 11 |
| 14 | Construction of HCV-core gene vector and its expression in cholangiocarcinoma显示文摘AIM: To establish an experimental model for exploring therole of hepatitis C vivus (HCV) in the development ofcholangiocarcinoma.METHODS: Recombinant plasmid of HCV-core gene wesconstructed with molecular cloning technique andtransfected into QBC939 cells with lipofection. After it wesselected with G418, resistant colonies were obtained. Thecolonies were analysed by immunocytochemistry andWestern blotting. The morphology was observed undertransmission electron microscope (TEM) and microscope.RESULTS: The recombinant plasmid wes proved to carry thetarget gene by PCR and restriction enzymed mapping.Moreover, it could express HCV-C protein efficiently inQBC039 cells. The HCV-like particles were found in thecytoplasm by EM, which were spherical with a diameter of50nr-80nm possessing outer membrane. The transfectedcells had lower differentiation and higher malignant degreeunder microscope.CONCLUSION: Because HCV-core gene could expresssteadily in cholangiocarcinoma cells, the transfected tumorcells(QBC939-HCVC) could be used to study the effect ofHCV in the development of cholangiocarcinoma. | Xiao-Fang Liu Sheng-Quan Zou Fa-Zu Qiu Department of General Surgery of Tongji Hospital,Wuhan 430030,Hubei Province,China | 2002 | World Journal of Gastroenterology2002，8，1： | 10 |
| 15 | Hepatocyte transformation and tumor development induced by hepatitis C virus NS3 c-terminal deleted protein显示文摘AIM: To study the effect of hepatitis C virus nonstructural protein 3 c-terminal deleted protein (HCV NS3-5') on hepatocyte transformation and tumor development.METHODS: QSG7701 cells were transfected with plasmid pRcHCNS3-5' (expressing HCV NS3 c-terminal deleted protein) by lipofectamine and selected in G418. The expression of HCV NS3 gene and protein was determined by PCR and immunohistochemistry respectively. Biological behavior of transfected cells was observed through cell proliferation assay, anchorage-independent growth and tumor development in nude mice. The expression of HCV NS3 and c-mycproteins in the induced tumor was evaluated by immunohistochemistry.RESULTS: HCV NS3 was strongly expressed in QSG7701 cells transfected with plasmid pRcHCNS3-5' and the positive signal was located in cytoplasm. Cell proliferation assay showed that the population doubling time in pRcHCNS3-5' transfected cells was much shorter than that in pRcCMV and nontransfected cells (24 h, 26 h, 28 h respectively). The cloning ratio of cells transfected with pRcHCNS3-5; pRcCMV and nontransfected cells was 33 %, 1.46 %, 1.11%, respectively,the former one was higher than that in the rest two groups (P<0.01). Tumor development was seen in nude mice inoculated with pRcHCNS3-5' transfected cells after 15 days.HE staining showed its feature of hepatocarcinoma, and immunohistochemistry confirmed the expressions of HCV NS3and c-mycproteins in tumor tissue. The positive control group inoculated with HepG2 also showed tumor development, while no tumor developed in the nude mice injected with pRcCMV and non-transfected cells after 40 days.CONCLUSION: 1.HCV NS3 c-terminal deleted protein has transforming and oncogenic potential. 2. Human liver cell line QSG7701 may be used as a good model to study HCV NS3 pathogenesis. | Qiong-QiongHe Rui-XueCheng YiSun De-YunFeng Zhu-ChuChen HuiZheng | 2003 | World Journal of Gastroenterology2003，9，3： | 10 |
| 16 | 干扰素联合胸腺肽治疗慢性乙型肝炎显示文摘目的评价IFN-α联合胸腺肽治疗慢性乙型肝炎(CHB)的疗效和安全性,探讨两者联合治疗的协同作用。方法 HBV DNA及HBeAg阳性的CHB患者215例,随机分为A,B两组。A组采用IFN-α和胸腺肽联合治疗,IFN-α3~5MU,1次·d^1im,15d后改为隔日一次im;胸腺肽100mg,1次·d^1静滴,1mo后改为20mg,1次·d^1im,疗程6mo,B组单用IEN-α,疗程6mo治疗结束后随访6mo,观察两组肝功能和乙肝病毒标志(HBVM及HBV DNA)的变化情况和不良反应。结果治疗结束时,两组临床症状均明显改善,A组中81例(63.3%)ALT恢复正常,而B组仅43例(49.4%)正常,两组有显著性差异(P<0.05);A组有70%HBV DNA阴转,59.4%HBeAg阴转,56.3%为近期完全应答(ALT复常,HBV DNA及HBeAg均阴转),B组分别为63.2%,48.3%和46.0%,两组无显著性差异(P>0.05)治疗结束后6mo,A组有65.6%ALT复常,68.8%HBV DNA阴转,66.4%HBeAg阴转,62.5%为持续完全应答;而B组则分别为31.0%,40.2%,37.9%和27.6%,两组有非常显著性差异(P<0.01)。治疗结束时已产生生化学和(或)病毒学应答的病例,随访期间A组有22.2%ALT又异常,23.3%HBV DNA再转阳,13.2%HBeAg再转阳,B组则分别为44.2%,41.8%和31.0%,B组生化学和病毒学复发率显著高于A组,具有显著性差异(P<0.05),治疗结束时无应答的病例中,随访期间A组有44.7%ALT复常,50% HBV DNA阴转,36.5% HBeAg阴转,而B组分别为6.8%,9.4%和8.9%,A组的生化学和病毒学滞后应答率显著高于B组,两组具有非常显著性差异(P均<0.01),治疗过程中,A组除出现与IFN-α治疗相关的初期副反应外,未发现其他副作用。结论 IFN-α与胸腺肽联合治疗CHB具有明显的协同作用,可明显改善肝功能,抗病毒效果好,明显优于单用IFN-α组,大大提高了IFN-α的远期疗效,使复发率明显降低,是CHB患者安全、有效的治疗方法,当然,62.5%的持续应答率远非理想效果,需要继续探索新的联合治疗方案。 | 游晶 庄林 唐宝璋 扬惠 扬微波 李武 张宏丽 张艳梅 张禄 严绍明 | 2001 | 世界华人消化杂志2001，9，4： | 9 |
| 17 | 丙型肝炎病毒核心蛋白对细胞凋亡的影响显示文摘目的考察丙型肝炎病毒核心蛋白对细胞凋亡的影响,以期从另一个角度研究丙型肝炎病毒可能的致癌机理.方法应用流式细胞仪检测 HepG2-C 细胞和 HepG2-CMV 细胞对氨甲喋呤(MTX 10mg/L)和去血清的耐受.并进一步检测凋亡相关基因及产物的表达.结果应用流式细胞仪检测 HepG2-C 细胞和 HepG2-CMV 细胞对氨甲喋呤(MTX 10mg/L)的耐受,结果 HepG2-C 细胞24小时和48小时的凋亡率分别为5.1%和9.6%,而 HepG2-CMV 细胞为6.8%和16%.而去血清2,4,6天的 HepG2-C 细胞凋亡率分别为6.6%,9.4%,11.5%,而 HepG2-CMV 细胞分别为11.4%、13.5%、16.1%.表明表达丙肝病毒核心蛋白的细胞 HepG2-C 较转染空载体的细胞 HepG2-CMV 耐受凋亡.在进一步分子机理的探讨中发现 HepG2-C 细胞 P53,C-myc,Bcl-2表达增加,而 Fas 表达减低,转录水平也发生同样的变化.结论丙肝病毒核心蛋白可能通过调节基因转录与表达,抑制某些因素引起的细胞凋亡. | 刘重阳 刘为纹 陈东风 杨建民 房殿春 | 2001 | 世界华人消化杂志2001，9，10： | 9 |
| 18 | KHI1正反义基因对MHCC97-H肝癌细胞KAI1蛋白表达的影响显示文摘目的:构建KAI1基因正、反义真核表达质粒,了解其对高转移潜能的MHCC97—H肝癌细胞KAI1蛋白表达的影响.方法:利用亚克隆技术构建KAI1基因正、反义真核表达质粒,并用脂质体法将其分别转入高转移潜能的MHCC97-H肝癌细胞系,通过免疫细胞化学SP法检测KAI1蛋白表达情况。结果:限制性内切酶分析证明两个重组子的结构均与KAI1正、反义基因表达质粒的预期结构一致。免疫细胞化学SP法检测显示,转入正义KAI1基因后的肝癌细胞KAI1蛋白染色加深,(细胞积分光密度 integra oculus dehter,IOD20.127 ± 5.099 vs 12.675±1.921,P<0.01);而转入反义KAI1基因的肝癌细胞则KAI1蛋白染色变浅,(IOD 8.681±2.472 vs 12.675 ± 1.921,P<0.01).结论:成功构建了KAIl基因正、反义真核表达质粒.KAI1正义基因能上调肝癌细胞KAI1蛋白的表达,相反,KAI1反义基因则能下调肝癌细胞KAI1蛋白的表达. | 司遂海 杨建民 罗元辉 房殿春 周平 | 2003 | 世界华人消化杂志2003，11，9： | 8 |
| 19 | 肝硬化患者的血清瘦素水平显示文摘目的:研究肝硬化患者血清瘦素水平的变化,探讨其与肝功能及胰岛素水平的关系.方法:肝硬化组50例(男性40例,女性10例),对照组30例(男20例,女10例),用放射免疫分析法测定血清瘦素和胰岛素水平,同时测量反映营养状态的人体测量学指标(身高和体重),体重指数(BMI)=体重(kg)/身高2(m2).分析各组间瘦素的变化及其与各指标间的关系.结果:肝硬化组及对照组中女性的血清瘦素水平均显著高于男性(P<0.01).肝硬化组的血清瘦素、胰岛素水平显著高于对照组(P<0.01).随着肝功能的恶化,男性肝硬化患者的血清瘦素水平逐渐增高,A和B级、B和C级之间比较无显著性差异(P>0.05),A和C级之间差异有显著性(P<0.05).ChildA级的BMI与对照组比较差异显著(P<0.05),但ChildB、C级的BMI与对照组无显著性差异(P>0.05).随着肝功能的恶化,肝硬化组空腹血糖和胰岛素水平逐渐升高.在对照组,瘦素与体重、BMI、空腹血糖和胰岛素显著相关.在肝硬化组,瘦素与体重、BMI和胰岛素水平显著相关,而与空腹血糖无相关性.结论:肝硬化患者的血清瘦素升高,女性的瘦素水平高于男性,并随着肝功能的逐渐恶化而升高,与体重、BMI和胰岛素明显相关. | 王学清 张卫卫 李岩 | 2002 | 世界华人消化杂志2002，10，10： | 6 |
| 20 | 肝炎病毒感染在非霍奇金淋巴瘤发病中的作用显示文摘目的:探讨丙型肝炎病毒和乙型肝炎病毒感染在非霍奇金淋巴瘤发病中的作用。 方法:采用ELISA和反转录-聚合酶链反应的方法检测了74例非霍奇金淋巴瘤患者外周血的抗-HCV抗体和HCVRNA;同时检测了110名健康献血员中抗-HCV抗体和HCVRNA。用ELISA方法检测了74例非霍奇金氏淋巴瘤患者和110名献血员。同时采用聚合酶链反应对于非霍奇金淋巴瘤患者外周血淋巴细胞中Bcl-2/J_H基因重排情况进行了检测。 结果:在74例非霍奇金氏淋巴瘤中的抗-HCV抗体阳性的为4例,阳性率为5.3%,HCVRNA阳性者为6例,阳性率为8.1%;110名健康献血员的抗-HCV抗体的阳性率为0.9%;HCVRNA阳性率为1.8%。非霍奇金淋巴瘤中的抗-HCV抗体阳性率较健康献血员显著增高,而HCVRNA的阳性率也较健康献血员有增高。经x^2检验P>0.05,无显著性差异。在74例非霍奇金淋巴瘤中HBsAg阳性者有3例(4%),而在献血员中为2名(1.7%)。在74例非霍奇金淋巴瘤中有18例外周血检测出Pcl-2/J_H基因重排,且转位均发生在Bcl-2的主要断裂点区域(MBR)。经x^2 test P<0.01,在非霍奇金淋巴瘤中Bcl-2/J_H基因重排和HCV感染有明显的相关性。 结论:HCV和HBV的感染与非霍奇金氏淋巴瘤的发生可能不存在一定的相关关系。 | 李如琳 徐德忠 聂青和 闫永平 张景霞 | 2002 | 世界华人消化杂志2002，10，4： | 6 |
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