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| 1 | 青藏高原土壤中一株低温原油降解菌的分离鉴定及其原油降解特性显示文摘从青藏高原班戈桥地区土壤中分离到一株能利用原油为碳源生长的细菌(BGQ-6).通过16S rRNA基因序列比对及Biolog GENⅢ鉴定板确定该菌株为Rhodococcus qingshengii.将生长至对数期的菌株接入MM培养基,10℃、150 rpm条件下培养15 d后,通过GC法检测到该菌对原油的总降解率为74.14%,且对直链烷烃、支链烷烃、环烷烃和芳香烃等60种烃类有较高的降解率.通过特异性基因扩增检测到该菌株基因组中具有4个alk B和1个alm A两种烷烃羟化酶基因. | 王艺霖 艾雪 李师翁 龙昊知 章高森 张威 刘光琇 陈拓 | 2015 | 冰川冻土2015,37,2: | 5 |
| 2 | Soil particle and moisture-related factors determine landward distribution of bacterial communities in a lateral riverside continuum of the Xilin River Basin显示文摘Continuous landscape components along the lateral riverside are affected by both hydrologic connectivity and disconnectivity.In recent years,anthropogenic activities and climate changes have caused wetland shrinkage and land degradation along the lateral riverside of many arid and semiarid regions.Since microorganisms are major drivers of soil biochemical cycling,it is essential to examine soil microbial communities along the lateral landscape continuum to understand their ecosystem functioning and predict future land changes.Here,we collected samples along a lateral riverbed center-riverbed edge-oxbow lake-floodplain-terrace continuum(i.e.,landward distribution)in the Xilin River Basin,Inner Mongolia,China.The floodplain had the highest microbial diversity and heterogeneity,with Bacteroidetes,β-andγ-Proteobacteria being the most abundant taxa.In contrast,the terrace had the lowest microbial diversity and heterogeneity,with Acidobacteria,Actinobacteria,Verrucomicrobia,Gemmatimonadetes,andα-Proteobacteria as the most abundant taxa.Silt particle,salinity,and moisture were the most influential factors for landward variation of bacterial communities along the riverside continuum.Altogether,we demonstrate that dominant bacterial lineages,soil particles,and moisture-related factors are valuable indicators of this continuum,which can be leveraged for the early prediction of drought-induced wetland shrinkage and grassland desertification. | Jingli Yu Jingjing Xia Qiaoli Ma Chi Zhang Ji Zhao Xininigen Tanggood Yunfeng Yang | 2021 | Soil Ecology Letters2021,3,4: | 3 |
| 3 | 青藏高原东北部湖泊细菌群落结构特征季节性差异及驱动机制显示文摘微生物是湖泊生态系统重要的有机组分。了解不同时期青藏高原湖泊细菌群落特征、环境驱动因子及其群落构建过程的差异,对高原湖泊水生态管理具有重要的指导意义。分别于2020年6和12月采集青藏高原东北部5个湖泊水样,基于高通量测序技术、统计分析和模型分析,解析高原湖泊群在非冰封期和冰封期细菌群落结构、环境驱动因子和中性过程、确定性过程以及随机性过程在细菌群落构建过程的贡献。结果表明:(1)非冰封期湖泊细菌群落的Chao1丰度指数、Simpson和Shannon多样性指数低于冰封期,Spearman相关性分析结果表明非冰封期细菌群落的Chao1丰度指数和多样性指数与水温和海拔显著相关,而冰封期细菌群落的Chao1指数和多样性指数与总氮显著相关;(2)水体细菌群落的优势门均为变形菌门(Proteobacteria),非冰封期细菌中拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)和厚壁菌门(Firmicutes)的平均相对丰度明显高于冰封期。另外,主坐标分析和相似性分析结果表明非冰封期和冰封期的细菌群落组成差异极显著;(3)冗余分析结果表明不同时期湖泊群细菌群落结构的驱动因子不同,非冰封期环境因子影响的大小顺序为:总氮>纬度>总溶解固体>经度>溶解有机碳,冰封期为:海拔>溶解氧>溶解有机碳>pH;(4)Sloan中性模型计算结果表明冰封期高原湖泊细菌群落比非冰封期更易受到中性过程的驱动,并且冰封期细菌群落的迁移率也大于非冰封期(冰封期:0.289;非冰封期:0.130),反映冰封期细菌菌群扩散能力较强。随机性的扩散限制过程是非冰封期(贡献率=54.74%)和冰封期(贡献率=55.56%)湖泊细菌群落的主要构建过程。本文解析了不同时期高原湖泊细菌群落特征及其驱动机制,为高原湖泊水资源保护和管理以及深入理解细菌群落构建过程提供了基础。 | 王丹丹 黄跃飞 杨海娇 | 2023 | 湖泊科学2023,35,1: | 2 |
| 4 | 青藏高原唐古拉哈日钦冰芯表层和深层可培养细菌特征显示文摘对唐古拉哈日钦冰芯表层0.04~3.04m及深层127.44~130.36m中可培养细菌进行了对比研究。发现表层和深层可培养细菌数没有表现出显著差异性,表层冰芯中可培养细菌数为0~9.8×10^3CFU·mL^-1,略高于深层冰芯的0~8.4×10^3CFU·mL^-1。表层冰芯中微生物总数为10^3~10^6cells·mL^-1,深层冰芯中微生物总数为10^2~10^3cells·mL^-1。冰芯中可培养细菌属于Firmicutes(厚壁菌门)、Proteobacteria(变形菌门)、Actinobacteria(放线菌门)、Bacteroidetes(拟杆菌门)和Deinococcus-Thermus(异常球菌门)5大类。但表层和深层可培养细菌属于不同的优势门,表层为Proteobacteria(38%),深层为Firmicutes(42%)。表层和深层优势属均为Bacillus(芽孢杆菌属),占比分别为18%和29%。已有研究表明哈日钦冰芯98.8m达到了公元1000年的历史记录,因此对比千年尺度上可培养细菌数量及多样性的差异,能够为进一步发掘新基因,丰富微生物多样性,为了解可培养细菌的进化历史奠定基础。 | 王凤 王宁练 徐柏青 沈亮 顾政权 刘晓波 | 2019 | 冰川冻土2019,41,4: | 2 |
| 5 | Review on microorganisms of glacial snow and ice on the Tibetan Plateau显示文摘Over the past 15 years there has been much effort invested in microorganisms of glacial snow and ice on the Tibetan Plateau.These studies include:phenotypic characteristics of recovered isolates;factors(dust,temperature,altitude)influencing microbial abundance,diversity and community in one glacier;distribution of bacterial number,diversity,community along ice core depth;similarities and differences of regionally distributed ice core isolates;seasonal variation of bacterial abundance and diversity.The following need further study.Better methods for more information about the diversity,survival mechanism of glacial microorganism;more research about archaea and fungi;microbial resources;relation of glacial microorganisms with biogeochemical cycle and mass balance;research on altitude distribution. | ZHANG Shuhong HOU Shugui QIN Xiang YAN Peiying ZHAO Longfei LIANG Feng | 2013 | 商丘师范学院学报2013,29,12: | 0 |
| 6 | 西风和季风影响区冰川雪中细菌数量和群落组成与气候环境关系显示文摘青藏高原冰川是全球变化研究的热点地区,但对高原冰雪细菌与气候环境之间的关系还缺乏研究。选取西风影响下的新疆慕士塔格冰川、木吉冰川和印度季风影响下的尼泊尔雅拉冰川进行冰雪细菌研究,以揭示西风和季风影响区冰川雪中细菌丰度和群落组成特征以及细菌与区域气候环境的关系。研究发现:受西风影响的慕士塔格冰川和木吉冰川的主要细菌类群为Bacteroidetes和Betaproteobacteria;受印度季风影响的雅拉冰川的细菌优势类群为Betaproteobacteria和Cyanobacteria。表明西风和季风影响区冰川雪中细菌具有不同的群落组成。此外,通过比对16S rRNA,发现三个冰川分离的细菌与分离自海洋、湖泊、土壤、沙漠等寒冷环境的细菌具有很高的相似度。位于西风带的慕士塔格和木吉冰川雪中细菌Shannon指数高于位于印度季风区的雅拉冰川。印度季风带的雅拉冰川细菌群落组成受季节的影响明显而西风带的慕士塔格冰川则受季节影响比较小。 | 刘晓波 邢婷婷 刘勇勤 徐柏青 赵华标 王宁练 沈亮 | 2019 | 冰川冻土2019,41,1: | 0 |
| 7 | Biogeochemistry and geomicrobiology in extreme environments:Preface显示文摘1.Introduction Geomicrobiology as an interdisciplinary area has achieved great progress during the past two decades(Dong and Yu,2007). Geomicrobiology involves the study of microbes in a number of fundamental geological processes,both in the past and | Hongchen Jiang hongchen.jiang@gmail.com (Guest Editor) | 2012 | Geoscience Frontiers2012,3,3: | 0 |