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| 1 | bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis显示文摘Iron(Fe)deficiency is prevalent in plants grown in neutral or alkaline soil.Plants have evolved sophisticated mechanisms that regulate Fe homeostasis,ensuring survival.In Arabidopsis,FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(FIT)is a crucial regulator of Fe-deficiency response.FIT is activated indirectly by basic helix-loop-helix(bHLH)IVc transcription factors(TFs)under Fed eficiency;how ever,it remains unclear which protein(s)act as the linker to mediate the activation of FIT by bHLH IVc TFs.In this study,we characterize the functions of bHLH121 and demonstrate that it directly associates with the FIT promoter.We found that loss-of-function mutations of bHLH121 cause severe Fedeficiency symptoms,reduced Feaccum ulation,and disrupted expression of genes associated with Fehomeostasis.Genetic analysis showed that FIT is epistatic to bHLH121 and FIT overexpression partially rescues the bhlh121 mutant.Further investigations revealed that bHLH IVc TFs interact with and promote nuclear accumulation of bHLH121.We demonstrated that bHLH121 has DNA-binding activity and can bind the prom oters of the FIT and bHLHlb genes,but we did not find that it has either direct transcriptional activation or repression activity tow ard these genes.Meanw hile,we found that bHLH121 functions downstream of and is a direct target of bHLH IVc TFs,and its expression is induced by Fe deficiency in a bHLH IV c-dependent manner.Taken together,these results establish that bHLH121 functions together with bHLH IVc TFs to positively regulate the expression of FIT and thus plays a pivotal role in maintaining Fe homeostasis in Arabidopsis. | Rihua Lei Yang Li Yuerong Cai Chenyang Li Mengna Pu Chengkai Lu Yujie Yang Gang Liang | 2020 | Molecular Plant2020，13，4： | 14 |
| 2 | 我国作物养分高效研究的现状与未来发展趋势显示文摘养分是作物生长发育的基础,探明作物高效吸收、利用土壤养分的生理和分子机理是培育高产高效作物新品种的基础,对保护环境和实现我国农业的可持续性发展具有重要的理论意义和现实意义。本文就过去10年中我国在主要农作物养分高效吸收、利用的生理及分子机理研究方面取得的主要研究进展进行了综述,并对该研究领域的未来发展趋势进行了探讨。 | 凌宏清 袁力行 | 2016 | 中国基础科学2016，18，2： | 10 |
| 3 | Two soybean bHLH factors regulate response to iron deficiency显示文摘Iron is an indispensable micronutrient for plant growth and development. Limited bioavailability of Fe in the soil leads to iron deficiency chlorosis in plants and yield loss. In this study, two soybean basic helix-loophelix transcription factors, Gmb HLH57 and Gmb HLH300,were identified in response to Fe-deficiency. Both transcription factors are expressed in roots and nodules,and are induced by Fe deficiency; these patterns were confirmed in transgenic hairy roots expressing constructs of the endogenous promoters fused to a GUS reporter gene. Bimolecular fluorescence complementation, yeast two-hybrid and coimmunoprecipitation(co-IP) assays indicated a physical interaction between Gmb HLH57 and Gmb HLH300. Studies on transgenic soybeans Reseoverexpressing Gmb HLH57 and Gmb HLH300 revealed that overexpression of each transcription factor, alone,results in no change of the responses to Fe deficiency,whereas overexpression of both transcription factors upregulated the downstream Fe uptake genes and increased the Fe content in these transgenic plants.Compared to wild type, these double overexpression transgenic plants were more tolerant to Fe deficiency.Taken together, our findings establish that Gmb HLH57 and Gmb HLH300 are important transcription factors involved in Fe homeostasis in soybean. | Lin Li Wenwen Gao Qi Peng Bin Zhou Qihui Kong Yinghui Ying Huixia Shou | 2018 | Journal of Integrative Plant Biology2018，60，7： | 10 |
| 4 | 机理Ⅰ植物铁营养的吸收转运及信号调控机制研究进展显示文摘铁是植物正常生长发育必需的微量元素之一。在通气良好的碱性或石灰性土壤中,常常因铁有效性低而难以满足植物生长发育所需,缺铁已成为继缺氮和缺磷之后农业生产所面临的又一重要的营养障碍因子。与机理Ⅱ植物相比,机理Ⅰ植物更易缺铁,因此全面了解机理Ⅰ植物的铁吸收及利用机制是分子育种改良此类植物铁营养的重要基础。基于国内外的相关研究进展,从机理Ⅰ植物的根际铁活化、根系铁吸收、木质部和韧皮部中的铁运输以及胞外和胞内铁的再利用等几方面进行综述;在此基础上,从bHLH和MYB转录因子调控网络、蛋白的泛素化修饰以及小分子化学信号调控途径等几方面,重点阐述机理Ⅰ植物铁营养吸收、转运及稳态平衡过程的调控机制;同时,对研究中存在的部分问题及未来研究方向进行简要的讨论分析。 | 张林琳 刘星星 祝亚昕 金崇伟 | 2021 | 植物营养与肥料学报2021，27，7： | 9 |
| 5 | The central circadian clock proteins CCA_1 and LHY regulate iron homeostasis in Arabidopsis显示文摘Circadian clock is the endogenous timekeeping machinery that synchronizes an organism's metabolism, behavior, and physiology to the daily lightdark circles, thereby contributing to organismal fitness.Iron(Fe) is an essential micronutrient for all organisms and it plays important roles in diverse processes of plant growth and development. Here, we show that, in Arabidopsis thaliana, loss of the central clock genes,CIRCADIAN CLOCK ASSOCIATED 1(CCA_1) and LATE ELONGATED HYPOCOTYL(LHY), results in both reduced Fe uptake and photosynthetic efficiency, whereas CCA_1 overexpression confers the opposite effects. We show that root Fe(III) reduction activity, and expression of FERRIC REDUCTION OXIDASE 2(FRO_2) and IRON-REGULATED TRANSPORTER 1(IRT_1) exhibit circadian oscillations, which are disrupted in the cca_1 lhy double mutant. Furthermore,CCA_1 directly binds to the specific regulatory regions of multiple Fe homeostasis genes and activates their expression. Thus, this study established that, in plants,CCA_1 and LHY function as master regulators that maintain cyclic Fe homeostasis. | Gang Xu Zhimin Jiang Haiyang Wang Rongcheng Lin | 2019 | Journal of Integrative Plant Biology2019，61，2： | 6 |
| 6 | 策略Ⅰ植物铁吸收稳态调控研究进展显示文摘铁是植物生长发育所必需的微量元素。作为辅酶因子和电子传递链组分,铁参与了光合作用、呼吸作用等多种重要的生理代谢过程。铁在地壳中的含量虽然丰富,但在中性和碱性土壤中大多以Fe^(3+)的形式存在,溶解度极低,限制了土壤中铁的生物有效性,导致植物生长发育易受缺铁影响,致使植物缺铁失绿已成为全世界普遍关注的问题。但在低pH和长期淹水条件下,植物会吸收累积过量的铁,产生活性氧,导致植物伤害甚至死亡。因此精确调控铁的吸收转运,保持体内铁稳态是植物生长发育的基础。本文就策略Ⅰ植物铁稳态调控方面的最新研究进展做一阶段性总结,并对存在的问题和未来的发展动态提出了作者的观点。 | 李文凤 朱海焰 兰平 | 2021 | 土壤2021，53，6： | 5 |
| 7 | bHLH104 confers tolerance to cadmium stress in Arabidopsis thaliana显示文摘Cd is a non-essential heavy metal that is toxic to both plants and animals. Here, we reveal that the transcription factor bHLH104 positively regulates Cd tolerance in Arabidopsis thaliana. We show that Fe deficiency-responsive genes were induced by Cd treatment, and that their upregulation was suppressed in bhlh104 loss-of-function mutants, but enhanced upon overexpression of bHLH104. Correspondingly, the bhlh104 mutants displayed sensitivity to Cd stress, whereas plants overexpressing bHLH104 exhibited enhanced Cd tolerance.Further analysis suggested that bHLH104 positively regulates four heavy metal detoxification-associated genes, IREG2, MTP3, HMA3 and NAS4, which play roles in Cd sequestration and tolerance. The bHLH104 overexpression plants accumulated high levels of Cd in the root but low levels of Cd in the shoot, which might contribute to the Cd tolerance in those lines. The present study thus points to bHLH104 as a potentially useful tool for genetic engineering of plants with enhanced Cd tolerance. | Xiani Yao Yuerong Cai Diqiu Yu Gang Liang | 2018 | Journal of Integrative Plant Biology2018，60，8： | 4 |
| 8 | Oryza sativa FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(OsFIT/OsbHLH156)interacts with OsIRO2 to regulate iron homeostasis显示文摘Iron(Fe)is indispensable for the growth and development of plants.It is well known that FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(FIT)is a key regulator of Fe uptake in Arabidopsis.Here,we identify the Oryza sativa FIT(also known as Osb HLH156)as the interacting partner of IRON-RELATED BHLH TRANSCRIPTION FACTOR 2(OsIRO2)that is critical for regulating Fe uptake.The OsIRO2 protein is localized in the cytoplasm and nucleus,but OsFIT facilitates the accumulation of OsIRO2 in the nucleus.Loss-of-function mutations of OsFIT result in decreased Fe accumulation,severe Fe-deficiency symptoms,and disrupted expression of Feuptake genes.In contrast,OsFIT overexpression promotes Fe accumulation and the expression of Fe-uptake genes.Genetic analyses indicate that OsFIT and OsIRO2 function in the same genetic node.Further analyses suggest that OsFIT and OsIRO2 form a functional transcription activation complex to initiate the expression of Fe-uptake genes.Our findings provide a mechanism understanding of how rice maintains Fe homeostasis. | Gang Liang Huimin Zhang Yang Li Mengna Pu Yujie Yang Chenyang Li Chengkai Lu Peng Xu Diqiu Yu | 2020 | Journal of Integrative Plant Biology2020，62，5： | 4 |
| 9 | Restriction of iron loading into developing seeds by a YABBY transcription factor safeguards successful reproduction in Arabidopsis显示文摘Iron(Fe)storage in plant seeds is not only necessary for seedling establishment following germination but is also a major source of dietary Fe for humans and other animals.Accumulation of Fe in seeds is known to be low during early seed development.However,the underlying mechanism and biological significance remain elusive.Here,we show that reduced expression of Arabidopsis YABBY transcription factor INNER NO OUTER(INO)increases embryonic Fe accumulation,while transgenic overexpression of INO results in the opposite effect.INO is highly expressed during early seed development,and decreased INO expression increases the expression of NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 1(NRAMP1),which encodes a transporter that contributes to seed Fe loading.The relatively high embryonic Fe accumulation conferred by decreased INO expression is rescued by the nramp1 loss-of-function mutation.We further demonstrated that INO represses NRAMP1 expression by binding to NRAMP1-specific promoter region.Interestingly,we found that excessive Fe loading into developing seeds of ino mutants results in greater oxidative damage,leading to increased cell death and seed abortion,a phenotype that can be rescued by the nramp1 mutation.Taken together,these results indicate that INO plays an important role in safeguarding reproduction by reducing Fe loading into developing seeds by repressing NRAMP1 expression. | Li Sun Yun Qi Wei Kang Hao Wu Jing Ying Yan Jie Na Xu Yun Rong Wu Gui Xin Li Ji Ming Xu Nicholas P.Harberd Zhong Jie Ding Shao Jian Zheng | 2021 | Molecular Plant2021，14，10： | 3 |
| 10 | Iron uptake, signaling, and sensing in plants显示文摘Iron (Fe) is an essential micronutrient that affects the growth and development of plants because it participates as a cofactor in numerous physiological and biochemical reactions. As a transition metal, Fe is redoxactive. Fe often exists in soil in the form of insoluble ferric hydroxides that are not bioavailable to plants.Plants have developed sophisticated mechanisms to ensure an adequate supply of Fe in a fluctuating environment. Plants can sense Fe status and modulate the transcription of Fe uptake-associated genes, finallycontrolling Fe uptake from soil to root. There is a critical need to understand the molecular mechanisms bywhich plants maintain Fe homeostasis in response to Fe fluctuations. This review focuses on recentadvances in elucidating the functions of Fe signaling components. Taking Arabidopsis thaliana and Oryzasativa as examples, this review begins by discussing the Fe acquisition systems that control Fe uptake fromsoil, the major components that regulate Fe uptake systems, and the perception of Fe status. Future explorations of Fe signal transduction will pave the way for understanding the regulatory mechanisms that underlie the maintenance of plant Fe homeostasis. | Gang Liang | 2022 | Plant Communications2022，3，5： | 3 |
| 11 | 梨叶片黄化复绿过程中bHLH转录因子的表达分析显示文摘为探究bHLH转录因子在梨叶缺铁黄化复绿过程中的表达特性,筛选响应该过程的关键bHLH基因,为改良梨品种抗缺铁能力提供理论依据。该试验以‘砀山酥梨’(Pyrus bretschneideri Rehd.)正常植株和黄化植株为试材,于生长期对黄化植株叶面喷施0.2%FeSO_(4)(2 g/L FeSO_(4))溶液,以清水处理正常植株(N)和黄化植株(C)为对照,取N、C的叶片和幼嫩根毛,以及处理3、6、9和12 d的黄化植株叶片进行转录组学分析,筛选其内差异表达的bHLH基因;采用荧光定量PCR技术,分析叶片和根系中bHLH基因的表达情况、生物学特性以及与叶内Fe^(2+)含量的相关性。结果表明:(1)转录组数据显示,于N、C和FeSO_(4)处理后不同时期黄化叶内共计获得21个表达差异显著的bHLH基因,涉及6个亚族,其motif数1~10不等。(2)qRT-PCR结果显示,C叶内10个基因(PbrbHLH7/29/41/104/119/122/128/155/183/191)的表达量均显著高于N叶内相应基因的表达量,FeSO_(4)处理后其各时期表达量均较C显著下调;而C叶内9个基因(PbrbHLH15/46/53/69/78/89/115/137/144)的表达量均显著低于N叶内相应基因的表达量,FeSO_(4)处理后其各时期表达量均较C显著上调。(3)根与地上部叶的表达水平差异相一致,C根内PbrbHLH29/41/119/128/155/183/191这7个基因的表达量显著高于N根内相应基因的表达量,而C根内PbrbHLH53/89的表达量显著低于N根内相应基因的表达量。(4)相关分析显示,FeSO_(4)溶液处理后各时期内叶片中Fe^(2+)含量与PbrbHLH41表达量呈显著负相关关系,而与PbrbHLH78呈显著正相关关系。研究认为,外源FeSO_(4)处理所调控的梨缺铁黄化叶复绿可能与其所导致的梨叶片样中bHLHs表达量协同变化密切相关;PbrbHLH41/78可能在该过程中发挥着至关重要而拮抗的调控作用,可作为研究梨缺铁黄化复绿机理的候选基因。 | 张舒琴 张海燕 于淼 胡小妹 邢杨天 刘伦 | 2022 | 西北植物学报2022，42，9： | 2 |
| 12 | AtHAP5A regulates iron translocation in iron-deficient Arabidopsis thaliana显示文摘Iron(Fe)deficient plants employ multiple strategies to increase root uptake and root-to-shoot translocation of Fe.The identification of genes that are responsible for these processes,and a comprehensive understanding of the regulatory effects of transcriptional networks on their expression,including transcription factors(TFs),is underway in Arabidopsis thaliana.Here,we show that a Histone-or heme-associated proteins(HAP)transcription factor(TF),HAP5A,is necessary for the response to Fe deficiency in Arabidopsis.Its ex-pression was induced under Fe deficiency,and the lack of HAP5A significantly decreased Fe translocation from the root to the shoot,resulting in substantial chlorosis of the newly expanded leaves,compared with the wild-type(WT,Col-0).Further analysis found that the expression of a gene encoding nicotianamine(NA)synthase(NAS1)was dramatically decreased in the hap5a mutant,regardless of the Fe status.Yeast-one-hybrid and ChIP analyses suggested that HAP5A directly binds to the promoter region of NAS1.Moreover,overexpression of NAS1 could rescue the chlorosis phenotype of hap5a in Fe deficient conditions.In summary,a novel pathway was elucidated,showing that NAS1-dependent translocation of Fe from the root to the shoot is controlled by HAP5A in Fe-deficient Arabidopsis thaliana. | Xiao Fang Zhu Qi Wu Yu Ting Meng Ye Tao Ren Fang Shen | 2020 | Journal of Integrative Plant Biology2020，62，12： | 2 |
| 13 | Glutamate synthase 1 is involved in iron-deficiency response and long-distance transportation in Arabidopsis显示文摘Iron is an essential microelement for plant growth.After uptake from the soil,iron is chelated by ligands and translocated from roots to shoots for sub-sequent utilization.However,the number of ligands in-volved in iron chelation is unclear.In this study,we identified and demonstrated that GLU1,which encodes a ferredoxin-dependent glutamate synthase,was involved in iron homeostasis.First,the expression of GLU1 was strongly induced by iron deficiency condition.Second,lesion of GLU1 results in reduced transcription of many iron-deficiency-responsive genes in roots and shoots.The mutant plants revealed a decreased iron concentration in the shoots,and displayed severe leaf chlorosis under the condition of Fe limitation,compared to wild-type.Third,the product of GLU1,glutamate,could chelate iron in vivo and promote iron transportation.Last,we also found that supplementation of glutamate in the medium can alleviate cadmium toxicity in plants.Overall,our results provide evidence that GLU1 is involved in iron homeo-stasis through affecting glutamate synthesis under iron deficiency conditions in Arabidopsis. | Man Cui Mengjun Gu Yaru Lu Yue Zhang Chunlin Chen Hong‐Qing Ling Huilan Wu | 2020 | Journal of Integrative Plant Biology2020，62，12： | 2 |
| 14 | Sequence Diversity and Enzyme Activity of Ferric-Chelate Reductase LeFRO1 in Tomato显示文摘Ferric-chelate reductase which functions in the reduction of ferric to ferrous iron on root surface is a critical protein for iron homeostasis in strategy I plants.LeFRO1 is a major ferric-chelate reductase involved in iron uptake in tomato.To identify the natural variations of LeFRO1 and to assess their effect on the ferric-chelate reductase activity,we cloned the coding sequences of LeFRO1 from 16 tomato varieties collected from different regions,and detected three types of LeFRO1(LeFR01^(MM),LeFRO1^(Ailsa) and LeFRO1^(Monita)) with five amino acid variations at the positions 21,24,112,195 and 582.Enzyme activity assay revealed that the three types of LeFRO1 possessed different ferric-chelate reductase activity(LeFRO1^(Ailsa)>LeFRO1^(MM)>LeFRO1^(Monita)).The 112th amino acid residue Ala of LeFRO1 is critical for maintaining the high activity of ferric-chelate reductase,because modification of this amino acid resulted in a significant reduction of enzyme activity.Further,we showed that the combination of the amino acid residue Ile at the site 24 with Lys at the site 582 played a positive role in the enzyme activity of LeFRO1.In conclusion,the findings are helpful to understand the natural adaptation mechanisms of plants to iron-limiting stress,and may provide new knowledge to select and manipulate LeFRO1 for improving the iron deficiency tolerance in tomato. | Danyu Kong Chunlin Chen Huilan Wu Ye Li Junming Li Hong-Qing Ling | 2013 | Journal of Genetics and Genomics2013，40，11： | 2 |
| 15 | bHLH转录因子在植物缺铁调控网络中的作用机制显示文摘铁是植物生长发育所需最重要的微量元素之一。其作为酶辅助因子或电子传递链的组成部分,在植物光合作用、呼吸作用和氨基酸生物合成等多种重要代谢过程中发挥作用。为应对铁缺乏的情况,植物进化出复杂的转录调控网络来维持铁的动态平衡,严格控制铁的吸收、运输、同化和储存。植物调控网络由多种转录因子参与构成,碱性螺旋-环螺旋(basic helix-loop helix, bHLH)家族是其中最关键的转录因子家族之一。本综述对植物响应铁缺乏的2种策略进行了简要概述,介绍了bHLH转录因子的结构、分类与作用形式,并重点讨论了类缺铁所诱导转录因子(ferritin-like iron deficiency-induced transcription factor, FIT)、PYE (POPEYE)、铁转运蛋白1上游调控因子(upsteam regulator of iron-regulated transporter 1,URI)等bHLH转录因子以及缺铁调控蛋白E3泛素连接酶BTS (BRUTUS)在铁稳态调控级联调控中作用的最新进展,以期为bHLH转录因子在植物缺铁应答调控网络中的作用机制研究提供理论基础。 | 赵安娜 罗光明 罗扬婧 宋丹丹 夏鸿东 任洪曼 张攀 | 2021 | 农业生物技术学报2021，29，12： | 2 |
| 16 | 一个响应土壤缺铁拟南芥突变体的分离及鉴定显示文摘铁是植物生长发育必需的一种微量元素,但土壤中植物可直接吸收利用的铁非常有限。缺铁使作物生长受限,进而影响人类膳食健康。植物体能通过调节一系列基因表达的变化来响应缺铁,但目前对该调控系统的研究仍不完善。CYP82C4(At4g31940)是拟南芥中一个强烈响应缺铁的基因,本研究将该基因启动子连接荧光素酶报告基因LUC2并转化拟南芥,进一步通过T-DNA的随机插入得到一个响应缺铁信号的突变体库。通过筛选该突变体库,我们得到一个强烈响应缺铁信号的突变体L22-8。和野生型相比,正常情况下L22-8地上部和地下部内源CYP82C4的表达量均显著增高,缺铁处理时其地上部表达量仍高于野生型,而地下部则不明显。定量结果显示FIT,b HLH38和b HLH39等植物铁代谢关键调控因子的表达发生了显著变化,但植株总铁、磷、锌的含量较野生型并没有显著区别,表明该T-DNA的插入虽影响了植株对缺铁胁迫的响应,但并不直接作用于植株对铁的吸收、转运上。反向PCR分析发现L22-8的T-DNA插入位点位于At3g51950和At3g51960之间,且这两个基因的转录表达在正常生长条件下均略低于Col-0。基因互补实验发现仅有At3g51960能部分互补L22-8的荧光信号,表明At3g51960基因的表达影响了CYP82C4对缺铁胁迫的响应。本研究进一步扩展了植物吸收利用铁的分子调控网络,为分子育种工作提供了指导。 | 王立赛 闫明科 王晗 沈仁芳 兰平 | 2018 | 土壤2018，50，3： | 2 |
| 17 | 草莓FaFIT在拟南芥中异源表达促进根系铁吸收显示文摘【目的】植物根系吸收铁受到碱性螺旋-环-螺旋(basic helix-loop-helix, bHLH)转录因子的调控,bHLH转录因子FaFIT在草莓中的功能未知,试验初步分析FaFIT基因在草莓根系铁吸收过程中调控作用。【方法】以红颜草莓为试材,基于根系缺铁胁迫转录组数据生物信息学分析结果,克隆草莓根系铁吸收运转调控基因FaFIT;结合实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)分析该基因在不同组织中的表达差异,通过转基因拟南芥进一步验证该基因的功能。【结果】从红颜草莓根系中克隆获得FaFIT基因序列,该基因mRNA编码区序列全长1020 bp,编码339个氨基酸。蛋白结构预测显示,FaFIT蛋白具有保守的HLH区域,属于bHLH转录因子家族。FaFIT基因启动子序列具有bHLH转录因子DNA结合元件、脱落酸(abscisic acid,ABA)等激素调控元件及干旱胁迫诱导元件,推测FaFIT基因可能受到上游b HLH转录因子、ABA和干旱等因素的调控和诱导。qRT-PCR分析显示,FaFIT基因在根系中特异性表达;FaFIT基因在根组织中受到缺铁、高pH值诱导后表达上调,但在铁过量胁迫时表达显著下调。通过FaFIT基因转化拟南芥,结果发现阳性株根系中与铁吸收相关的3个基因AtAHA2、AtFRO2和AtIRT1的表达水平均上调,且与3个基因相对应蛋白酶H~+-ATPase(P-type)、铁还原酶(ferric-chelate reductase, FCR)、铁转运体(iron-regulated transporter,IRT)的酶活性均提高。同时,在pH=5.8和pH=8.0的1/2 MS培养基中转FaFIT基因拟南芥阳性株系根部Fe2+积累显著增加,叶片叶绿素含量提高。【结论】FaFIT基因通过调控AtAHA、AtFRO2和AtIRT1基因表达以及增强H+-ATPase(ptype)、FCR和IRT酶活性促进根系铁的吸收和积累,推测是草莓根系铁吸收和转运过程中的关键调控因子。 | 陈亚铎 宋艳红 李刚 赵霞 刘丽锋 周厚成 | 2022 | 果树学报2022，39，9： | 2 |
| 18 | 植物铁还原酶基因FRO的研究进展显示文摘铁(Fe)是植物生长必需的微量元素,适宜的铁含量有利于植物的正常生长和发育。在吸收铁这一生理过程中,禾本科植物和非禾本科植物吸收铁的价态不同,并且铁在植物体内的运输过程也存在着二价与三价铁的相互转化。铁还原酶基因(Fe^3+chelate reductase,FRO)具有将三价铁还原成二价铁的功能。因此,在分子水平上研究FRO的具体功能具有非常重要的意义。综述了拟南芥、番茄、大豆、水稻、花生及蒺藜苜蓿等FRO基因在亚细胞定位、还原对象、诱导条件及调控或影响因素等方面的研究进展,以期为后续研究铁的吸收机制奠定理论基础。 | 乔孟欣 李素贞 陈景堂 | 2019 | 生物技术通报2019，35，7： | 2 |
| 19 | 植物bHLH转录因子调控铁稳态的研究进展显示文摘铁是植物生长发育所必需的微量营养元素,生长在中性或碱性土壤中的植物普遍存在缺铁现象。而植物的正常生长发育需要保持体内铁的平衡,这种铁稳态在转录和转录后水平上都受到严格的调控。植物中铁稳态的调控网络由许多转录因子参与,其中碱性螺旋-环-螺旋(basic helix-loop helix,bHLH)家族的成员不可或缺。本文拟对植物中调控铁平衡的关键bHLH转录因子进行梳理汇总,对这些转录因子在植物生长发育中调节铁稳态的机制进行综述,以期为揭示植物铁稳态调节的研究提供理论基础。 | 李宇 李素贞 陈茹梅 卢海强 | 2023 | 生物技术通报2023，39，7： | 1 |
| 20 | 柑橘主要砧木品种耐缺铁特性及FRO基因启动子特性分析显示文摘分析了目前柑橘果园中常用砧木以及具砧木价值的17个柑橘砧木品种的耐缺铁特性,筛选出缺铁响应较强的铁螯合还原酶基因(FRO),分析了不同品种中受缺铁诱导铁螯合还原酶基因的上游调控区序列特征和缺铁诱导特性.结果表明:17个柑橘砧木品种中枳柚、资阳香橙和枳雀为耐缺铁类型,莽山野柑、红橘、朱橘、酸橘、扁平橘、土橘、枳、兴义大红袍和皱皮橘属于缺铁敏感型,枳橙、汕头酸橘、兰卜莱檬、枸头橙和黄木黎檬属于中间型.在克里曼丁橘(Citrus clementina)基因组中CcFRO1,CcFRO2和CcFRO3基因表达受缺铁诱导明显,在柑橘砧木品种中,CcFRO1和CcFRO3基因的上游调控序列存在较大差异.缺铁条件下,耐受型启动子在植株中表达特性发生明显改变,而敏感型启动子表达特性则不受缺铁影响.研究结果表明,柑橘砧木耐缺铁特性可能与铁螯合还原酶基因上游调控区有关,为选育耐缺铁柑橘砧木品种提供了理论基础. | 张梅 王金娟 高美美 王莹 罗添鸣 冯邻 罗小英 李德谋 | 2022 | 西南大学学报(自然科学版)2022，44，10： | 0 |
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