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| 1 | Successful Production of an All-Female Common Carp (Cyprinus carpio L.) Population Using cyp17a1-Deficient Neomale Carp显示文摘Due to sexual dimorphism in the growth of certain cultured fish species,the production of monosex fishes is desirable for the aquaculture industry.Nowadays,the most widely practiced technique available for the mass production of monosex fish populations is sex steroid-induced sex reversal.Here,a novel strategy for the successful production of all-female(AF)common carp(Cyprinus carpio L.),to take advantage of the sexual dimorphism in growth documented in this species,has been developed using genetic engineering via single gene-targeting manipulation without any exogenous hormone treatments.Male and female heterozygous cyp17a1-deficient common carp were first obtained using the clustered regularly interspaced short palindromic repeats/CRISPR-associated endonuclease 9(CRISPR/Cas9)technique.An all-male phenotype for homozygous cyp17a1-deficient carp,regardless of the individuals’sexdetermination genotypes(XY or XX),has been observed.A male-specific DNA marker newly identified in our laboratory was used to screen the neomale carp population with the XX genotype from the cyp17a1-deficient carp.These neomale carp develop a normal testis structure with normal spermatogenesis and sperm capacity.The neomale common carp were then mated with wild-type(WT)females(cyp17a1^(+/+)XX genotype)using artificial fertilization.All the AF offspring sample fish from the neomale-WT female mating were confirmed as having the cyp17a^(1+/-)XX genotype,and normal development of gonads to ovaries was observed in 100.00%of this group at eight months post-fertilization(mpf).A total of 1000 carp fingerlings,500 from the WT male and female and 500 from the neomale and WT female mating,were mixed and reared in the same pond.The average body weight of cyp17a1^(+/-)XX females was higher by 6.60%(8 mpf)and 32.66%(12 mpf)than that of the control common carp.Our study demonstrates the first successful production of a monosex teleost population with the advantages of sexual dimorphism in growth using genetic manipulation targeting a single locus. | Gang Zhai Tingting Shu Kuangxin Chen Qiyong Lou Jingyi Jia Jianfei Huang Chuang Shi Xia Jin Jiangyan He Donghuo Jiang Xueqiao Qian Wei Hu Zhan Yin | 2022 | Engineering2022，8，1： | 7 |
| 2 | Designing future farmed fishes using genome editing显示文摘Aquaculture,the most rapidly growing food production sector,has exhibited tremendous expansion over the past several decades and it currently accounts for more than half of the global fish production for human consumption.To ensure sustainable aquaculture,it is necessary to breed farmed fish species(strains)with valuable economic traits,such as high production,high quality,disease resistance,and stress tolerance.Modem fish genetic breeding techniques largely rely on key processes including understanding the genetic basis of economically important traits to the generation and selection of favorable genotypes. | Yonghua Sun Zuoyan Zhu | 2019 | Science China(Life Sciences)2019，62，3： | 6 |
| 3 | Genome editing opens a new era for physiological study and directional breeding of fishes显示文摘SPECIAL TOPIC:Fish Genome Editing In recent years,the whole life science community has witnessed the advance and breakthrough in the genome editing technologies,including zinc-finger nucleases(ZFNs),transcription activator-like effector(TALE)nucleases(TALENs)and clustered,regularly interspaced,short palindromic repeats(CRISPR)–CRISPR-associated(Cas)systems(CRISPR/Cas9)[1–3].With these tools。 | Sun, Yong-Hua | 2017 | Science Bulletin2017，62，3： | 5 |
| 4 | Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity显示文摘The endocrine regulatory roles of the hypothalamic-pituitary-adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa.However,the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone(ACTH),a pro-opiomelanocortin(POMC)-derived peptide,on animal energy expenditure and somatic growth remain unknown.POMC has also been identified as one of the candidate loci for polycystic ovary syndrome,which features hyperandrogenism and some prevalence of obesity in patients.Here we show that zebrafish lacking functional POMCa exhibit similar phenotypes of stress response and body weight gain but not obesity as observed in mammalian models.In contrast with the impaired anorexigenic signaling cascade of melanocyte-stimulating hormones and leptin,which are responsible for their obesity-prone weight gain observed in various pome mutant mammals,analyses with our pomca mutant series indicate that ACTH is the key regulator for the phenotype with enhanced somatic growth without obesity in pomca-deficient zebrafish.Hypocortisolism associated with hyperandrogenism has been observed in the pomca-deficient zebrafish,with enhanced activation of mammalian target of rapamycin complex 1;reutilization of amino acids and fatty acid^-oxidation are observed in the muscle tissue of the pomca-deficient fish.After reducing hyperandrogenism by crossing our pomca mutant fish with a cy p l 7a 1-deficient background,the phenotype of enhanced somatic growth in pomca-deficient fish was no longer observed.Thus,our work also demonstrated that the role of POMCa in stress response seems to be conserved in vertebrates,whereas its effect on adipostasis is unique to teleosts. | Chuang Shi Yao Lu Gang Zhai Jianfei Huang Guohui Shang Qiyong Lou Dongliang Li Xia Jin Jiangyan He Zhenyu Du Jianfang Gui Zhan Yin | 2020 | Journal of Molecular Cell Biology2020，12，4： | 1 |
| 5 | 鱼类雄性性别分化与精巢发育调控基因研究进展显示文摘鱼类性别受环境与基因共同调控,两者均可影响鱼类性腺分化、发育,但环境如何影响性腺发育基因来调控鱼类性别尚不明确。现有研究认为,上游基因sox9、amh高表达可诱导鱼类原始性腺分化为精巢,sox9促进精母细胞增殖,amh抑制精原细胞增殖,下游基因ar、er表达水平升高可诱导鱼类精原细胞分裂、分化、精母细胞增殖以及精子发生过程;外源雌激素抑制sox9、amh表达,对ar、er则表现为低浓度诱导,高浓度抑制。综上,鱼类雄性性别分化受sox9、amh、ar、er共同调控,环境因子的改变可影响这一分化过程。因此,本文对鱼类性别分化上游主要调控基因sox9和amh,下游主要调控基因ar和er以及它们与精巢发育的关系、环境因子胁迫对这些基因和精巢组织结构的影响展开综述,以探讨鱼类精巢发育以及环境因子对这一发育过程的基因调控作用。 | 杨超超 屈蓉 杜妙柔 王琪 雷忻 | 2023 | 中国实验动物学报2023，31，9： | 0 |
| 6 | 1,3-二苯胍对斑马鱼早期生命阶段的影响显示文摘1,3-二苯胍(1,3-diphenyl guanidine,DPG)是橡胶生产过程中添加的硫化促进剂,在水环境中广泛存在。然而作为水环境中的一种污染物,目前DPG对水生生物的毒性及其机制的研究极为匮乏。为了研究DPG对水生生物的生殖发育毒性及潜在机制,选择水生模式生物斑马鱼(Danio rerio)作为受试生物。将斑马鱼胚胎分别暴露于浓度为30、100和300μg·L^(-1)的DPG溶液中120 h,通过转录组测序、基因表达水平定量分析及性激素水平测定(雌二醇和睾酮)来探究其对斑马鱼早期生命阶段的生殖发育毒性及机制。转录组测序结果表明,DPG暴露可以显著影响与精卵识别及生殖细胞发育相关的生物学过程。DPG暴露使类固醇合成通路相关基因(cyp11a1、cyp17a1、cyp19a1a、hsd17b1、ar)的转录表达显著上调。激素结果表明DPG暴露导致斑马鱼仔鱼体内雌二醇水平显著升高。以上研究结果表明,在分子水平上,DPG能够影响斑马鱼胚胎及仔鱼的发育,但是在个体水平上仍然缺乏直接的证据揭示DPG暴露对斑马鱼生殖和胚胎发育的不良结局。本研究为DPG对水生生物的毒性研究奠定了一定的基础,并为轮胎磨损颗粒的毒性研究提供了新的线索。 | 赵娟 常静 郝伟玉 马政 宋哲媛 万斌 王会利 | 2023 | 生态毒理学报2023，18，4： | 0 |
| 7 | The application of genome editing technology in fish显示文摘The advent and development of genome editing technology has opened up the possibility of directly targeting and modifying genomic sequences in the field of life sciences with rapid developments occurring in the last decade.As a powerful tool to decipher genome data at the molecular biology level,genome editing technology has made important contributions to elucidating many biological problems.Currently,the three most widely used genome editing technologies include:zinc finger nucleases(ZFN),transcription activator like effector nucleases(TALEN),and clustered regularly interspaced short palindromic repeats(CRISPR).Researchers are still striving to create simpler,more efficient,and accurate techniques,such as engineered base editors and new CRISPR/Cas systems,to improve editing efficiency and reduce off-target rate,as well as a near-PAMless SpCas9 variants to expand the scope of genome editing.As one of the important animal protein sources,fish has significant economic value in aquaculture.In addition,fish is indispensable for research as it serves as the evolutionary link between invertebrates and higher vertebrates.Consequently,genome editing technologies were applied extensively in various fish species for basic functional studies as well as applied research in aquaculture.In this review,we focus on the application of genome editing technologies in fish species detailing growth,gender,and pigmentation traits.In addition,we have focused on the construction of a zebrafish(Danio rerio)disease model and high-throughput screening of functional genes.Finally,we provide some of the future perspectives of this technology. | Jianguo Lu Wenyu Fang Junrou Huang Shizhu Li | 2021 | Marine Life Science & Technology2021，3，3： | 0 |
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