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| 1 | 短链脂肪酸介导的肠上皮和抗炎调节研究进展显示文摘哺乳动物体中的肠道菌群是细菌生态系统的组成部分,从动物出生时起,这些微生物就对免疫系统的发育、功能和调节起着非常重要的作用。当前,越来越多的研究集中在微生物对宿主细胞代谢的影响上。短链脂肪酸(SCFA)作为肠道菌群的一种代谢产物,对肠道稳态的维持具有重要作用。SCFA是肠道上皮细胞的重要燃料,能增强肠屏障功能。作为信号分子,SCFA可以通过细胞表面G蛋白偶联受体(GPCR)发出信号,从而激活控制免疫功能的信号级联反应;还可以通过底物转运蛋白进入细胞,抑制组蛋白脱乙酰化酶(HDAC),最终达到降低肠道炎症反应。本文综述了微生物SCFA的产生及其对肠道黏膜的影响,并通过激活细胞表面GPCR以及抑制组蛋白去乙酰化酶(HDACs)来调节免疫反应的作用。 | 张晨 敖日格乐 王纯洁 斯木吉德 | 2021 | 中国畜牧杂志2021，57，1： | 9 |
| 2 | 前列腺癌诊断标志物研究进展显示文摘前列腺癌是严重危害男性健康的肿瘤,有较高的恶性倾向,在我国发病率逐年上升。前列腺癌患者生存和预后的关键在于早期诊断与有效的治疗。目前临床以前列腺特异性抗原(PSA)及穿刺活检为主要诊断方法,但存在特异度不高、过度穿刺活检等问题。寻找高特异度、高敏感度的前列腺癌诊断标志物是目前研究热点。近年研究发现了长链非编码RNA(lncRNA)、TMPRSS2:ERG融合基因等许多新的肿瘤诊断标志物,对PSA及前列腺癌基因3(PCA3)等诊断标志物的研究也有新的进展,本文针对前列腺癌诊断标志物研究进展作一综述。 | 洪西 刘利杰 俞建军 | 2018 | 临床泌尿外科杂志2018，33，12： | 7 |
| 3 | 前列腺癌中巴豆酰化修饰的测定及与肿瘤分期和分级的相关性显示文摘目的·初步探讨巴豆酰化修饰是否可以作为前列腺癌诊断的肿瘤标志物及其与前列腺癌分期和分级的相关性。方法·含有不同临床分期、分级的前列腺癌组织芯片73例,正常前列腺组织7例,利用免疫组织化学技术分别检测各样本中巴豆酰化修饰和组蛋白H4乙酰化修饰水平(H-Score评分),并进行组间比较和相关性分析。结果·巴豆酰化修饰水平在前列腺癌组织中较低,与正常前列腺组织比较,差异有统计学意义(P=0.001);而组蛋白H4乙酰化修饰水平组间比较差异无统计学意义(P=0.704)。不同临床分期、分级的前列腺癌组间比较,巴豆酰化修饰和组蛋白H4乙酰化修饰水平差异均无统计学意义(P>0.05)。H4乙酰化修饰与前列腺癌的分期、分级无相关性(P>0.05);巴豆酰化修饰与前列腺癌的分期无相关性(P=0.887),与前列腺癌分级呈正相关(r=0.493,P=0.000)。结论·相比较组蛋白H4乙酰化修饰,巴豆酰化修饰可以作为一种更好的诊断前列腺癌的肿瘤标志物。 | 刘利杰 洪西 黄先玉 罗静 俞建军 | 2018 | 上海交通大学学报(医学版)2018，38，7： | 5 |
| 4 | 从HBV cccDNA角度谈乙型肝炎的功能性治愈显示文摘HBV共价闭合环状DNA(cccDNA)是HBV转录和复制的模板,以微染色体的形式长期稳定存在于肝细胞内。笔者围绕cccDNA失活或清除机制及药物研发,介绍了对cccDNA形成、转录和降解等功能性事件的认识,并综述了cccDNA靶向药物及生物技术相关的研究进展。 | 陈娟 黄爱龙 | 2022 | 临床肝胆病杂志2022，38，8： | 4 |
| 5 | 蛋白质巴豆酰化修饰研究进展显示文摘赖氨酸巴豆酰化修饰作为蛋白翻译后修饰的一种,在染色质重塑、细胞代谢、细胞周期以及细胞重组中发挥着重要的作用。近年来,关于赖氨酸巴豆酰化修饰的研究越来越受科研工作者青睐。为进一步了解巴豆酰化修饰的生物学意义,就蛋白质巴豆酰化修饰在微生物、动物、人类和植物方面的研究现状进行综述,以期为植物病害的表观遗传学研究提供新的思路。 | 曹喜兵 赵振利 曹亚兵 翟晓巧 | 2020 | 河南林业科技2020，40，3： | 2 |
| 6 | Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence显示文摘Lysine 2-hydroxyisobutyrylation(Khib) is a newly identified post-translational modification(PTM) that plays important roles in transcription and cell proliferation in eukaryotes. However, its function remains unknown in phytopathogenic fungi. Here,we performed a comprehensive assessment of Khibin the rice false smut fungus Ustilaginoidea virens, using Tandem Mass Tag(TMT)-based quantitative proteomics approach. A total of 3 426 Khibsites were identified in 977 proteins, sugg esting that Khibis a common and complex PTM in U. virens. Our data demonstrated that the2-hydroxyisobutyrylated proteins are involved in diverse biological processes. Network analysis of the modified proteins revealed a highly interconnected protein network that included many well-studied virulence factors. We confirmed that the Zn-binding reduced potassium dependency3-type histone deacetylase(UvRpd3) is a major enzyme that removes 2-hydroxyisobutyrylation and acetylation in U. virens. Notably, mutations of Khib sites in the mitogen-activated protein kinase(MAPK)UvSlt2 significantly reduced fungal virulence and decreased the enzymatic activity of UvSlt2. Molecular dynamics simulations demonstrated that 2-hydroxyisobutyrylation in UvSlt2 increased the hydrophobic solvent-accessible surface area and thereby affected binding between the UvSlt2 enzyme and its substrates. Our findings thus establish Khibas a major post-translational modification in U. virens and point to an important role for Khibin the virulence of this phytopathogenic fungus. | Xiaoyang Chen Xiabing Li Pingping Li Xiaolin Chen Hao Liu Junbin Huang Chaoxi Luo Tom Hsiang Lu Zheng | 2021 | Journal of Integrative Plant Biology2021，63，2： | 2 |
| 7 | HDAC-dependent decrease in histone crotonylation during DNA damage显示文摘Dear Editor,The DNA damage response(DDR)ensures repair of DNA lesions caused by endogenous and exogenous mutagens that constantly threaten genomic integrity.Defective DDR results in accumulation of DNA lesions that could potentially lead to genomic instability and tumorigenesis.Posttranslational modifications(PTMs)including phosphorylation,methylation,acetylation,ubiquitination,and sumoylation play a central role in sensing,signaling,and repairing damaged DNA. | Enas R.A bu-Zhayia Feras E.Machour Nabieh Ayoub | 2019 | Journal of Molecular Cell Biology2019，11，9： | 1 |
| 8 | 赖氨酸巴豆酰化修饰与疾病的研究进展显示文摘近年来巴豆酰化修饰在生殖、发育和疾病等方面取得突破性进展而受到广泛关注。虽然巴豆酰化与乙酰化修饰部分相似,但其在生物学功能上仍有别于乙酰化修饰,提示巴豆酰化修饰对疾病可能发挥特异性调控作用。本文就组蛋白及非组蛋白巴豆酰化修饰与疾病的关系进行综述,以期为相关疾病的治疗和干预提供新思路。 | 肖星星 曾智 王莉 卢燕玲 周传传 曾海涛 孙晓红 | 2019 | 医学理论与实践2019，32，8： | 1 |
| 9 | Ustilaginoidea virens modulates lysine 2-hydroxyisobutyrylation in rice flowers during infection显示文摘The post-translational modification lysine 2-hydroxyisobutyrylation(K_(hib))plays an important role in gene transcription,metabolism,and enzymatic activity.K_(hib)sites have been identified in rice(Oryza sativa).However,the K_(hib)status of proteins in rice flowers during pathogen infection remains unclear.Here,we report a comprehensive identification of K_(hib)-modified proteins in rice flowers,and the changes in these proteins during infection with the fungal pathogen Ustilaginoidea virens.By using a tandem mass tag-based quantitative proteomics approach,we identified 2,891 K_(hib)sites on 964 proteins in rice flowers.Our data demonstrated that 2-hydroxyisobutyrylated proteins are involved in diverse biological processes.K_(hib)levels were substantially reduced upon infection with U.virens.Chromatin immunoprecipitation polymerase chain reaction(PCR)and reverse transcription quantitative PCR analyses revealed that histone K_(hib)is involved in the expression of disease-resistance genes.More importantly,most quantified sites on core histones H3 were downregulated upon U.virens infection.In addition,the histone deacetylases HDA705,HDA716,SRT1,and SRT2 are involved in the removal of K_(hib)marks in rice.HDA705 was further confirmed to negatively regulate rice disease resistance to pathogens U.virens,Magnaporthe oryzae,and Xanthomonas oryzae pv.oryzae(Xoo).Our data suggest that U.virens could modulate K_(hib)in rice flowers during infection. | Xiaoyang Chen Qiutao Xu Yuhang Duan Hao Liu Xiaolin Chen Junbin Huang Chaoxi Luo Dao-Xiu Zhou Lu Zheng | 2021 | Journal of Integrative Plant Biology2021，63，10： | 1 |
| 10 | 组蛋白赖氨酸巴豆酰化修饰的研究进展显示文摘组蛋白赖氨酸巴豆酰化修饰是2011年新发现的一种酰化修饰,对基因表达、细胞发育以及疾病治疗等生物过程具有重要的生物学意义。近两年内,巴豆酰化修饰生化相关研究取得突破性进展,尤其是在蛋白巴豆酰化修饰的生理功能以及与发育、遗传、疾病相关性等领域逐渐受到关注。现对蛋白巴豆酰化做一简要回顾,总结组蛋白巴豆酰化修饰鉴定、相关酶系统、识别结构域及生物学功能等,这些信息为将来进一步探索蛋白巴豆酰化的功能提供理论基础。 | 杨强震 李培飞 李新红 | 2017 | 生命科学2017，29，11： | 1 |
| 11 | 溴结构域蛋白4抑制剂对前列腺癌细胞组蛋白巴豆酰化修饰以及增殖、迁移的影响显示文摘目的·探讨溴结构域蛋白4(bromodomain-containing protein4,BRD4)抑制剂I-BET762对前列腺癌细胞组蛋白巴豆酰化修饰以及前列腺癌细胞增殖、迁移的影响。方法·选取3种人前列腺癌细胞系LNCaP、C4-2B和PC-3,分别以各自的半数抑制浓度的I-BET762处理,Western blotting检测组蛋白上巴豆酰化修饰以及乙酰化酶的表达。CCK-8、Transwell迁移实验和划痕实验分别测定I-BET762对于LNCaP、C4-2B和PC-3细胞的增殖、迁移的影响。结果·I-BET762可以抑制组蛋白乙酰化酶P300和GCN5的表达,降低组蛋白巴豆酰化修饰表达。Transwell迁移实验和划痕实验结果表明,I-BET762可以抑制前列腺癌细胞系LNCaP、C4-2B和PC-3的迁移能力(均P<0.01);CCK-8实验结果表明,I-BET762处理后,3种前列腺癌细胞系的增殖能力被抑制。结论·前列腺癌细胞中I-BET762能够降低组蛋白巴豆酰化修饰水平,抑制细胞增殖和迁移。 | 洪西 刘利杰 黄先玉 罗静 俞建军 | 2019 | 上海交通大学学报(医学版)2019，39，7： | 1 |
| 12 | HBV cccDNA转录调控机制与抗HBV治疗前景显示文摘以微染色体形式存在的HBV共价闭合环状DNA(cccDNA)在肝细胞核内持续稳定的存在,被认为是HBV感染慢性化、抗病毒治疗无法清除病毒以及停药后肝炎复发的最主要原因。然而,现有各种抗病毒治疗方案中,缺少以cccDNA本身或形成、转录等环节为靶点的药物,因此,靶向cccDNA环节的治疗策略是急需填补的空白。随着对cccDNA微染色体上组蛋白表观遗传修饰的逐步解析,表观遗传治疗也有望成为HBV新的潜在治疗方向。主要关注了HBV DNA甲基化和cccDNA组蛋白修饰的研究现状和发展方向,试图为HBV表观遗传治疗提供思路。 | 陈娟 黄爱龙 | 2019 | 临床肝胆病杂志2019，35，6： | 1 |
| 13 | The role of histone modifications:from neurodevelopment to neurodiseases显示文摘Epigenetic regulatory mechanisms,including DNA methylation,histone modification,chromatin remodeling,and microRNA expression,play critical roles in cell differentiation and organ development through spatial and temporal gene regulation.Neurogenesis is a sophisticated and complex process by which neural stem cells differentiate into specialized brain cell types at specific times and regions of the brain.A growing body of evidence suggests that epigenetic mechanisms,such as histone modifications,allow the fine-tuning and coordination of spatiotemporal gene expressions during neurogenesis.Aberrant histone modifications contribute to the development of neurodegenerative and neuropsychiatric diseases.Herein,recent progress in understanding histone modifications in regulating embryonic and adult neurogenesis is comprehensively reviewed.The histone modifications implicated in neurodegenerative and neuropsychiatric diseases are also covered,and future directions in this area are provided. | Jisu Park Kyubin Lee Kyunghwan Kim Sun-Ju Yi | 2022 | Signal Transduction and Targeted Therapy2022，7，8： | 1 |
| 14 | 炎症环境下组蛋白去乙酰化对牙周膜干细胞影响的研究现状显示文摘牙周炎是口腔常见疾病,目前无有效根治办法,牙周组织中牙周膜干细胞(PDLSCs)具有较高的增值能力和多分化潜能,但是在炎性微环境下PDLSCs再生能力减弱,而组蛋组蛋白去乙酰化酶(HDACs)可以调控基因表达,维持细胞正常功能,所以,检测炎性微环境下PDLSCs中HDACs的表达和HDACs对PDLSCs免疫调节特性的影响,并利用组蛋白去乙酰化抑制剂(HDACIs),检测其在炎症微环境下骨形成能力、PDLSCs成骨分化的变化。 | 李菊 范剑妩 | 2018 | 全科口腔医学电子杂志2018，5，31： | 0 |
| 15 | Insights into the post-translational modification and its emerging role in shaping the tumor microenvironment显示文摘More and more in-depth studies have revealed that the occurrente and development of tumors depend on gene mutation and tumor heterogeneity.The most important manifestation of tumor heterogeneity is the dynamic change of tumor microenvironment(TME)heterogeneity.This depends not only on the tumor cells themselves in the microenvironment where the infiltrating immune cells and matrix together forming an antitumor and/or pro-tumor network.TME has resulted in novel therapeutic in terve ntions as a place beyond tumor beds.The malig nant can cer cells,tumor in filtrate immune cells,angiogenic vascular cells,lymphatic endothelial cells,cancer-associated fibroblastic cells,and the released factors including intracellular metabolites,hormonal signals and inflammatory mediators all contribute actively to cancer progression.Protein post-translational modification(PTM)is often regarded as a degradative mechanism in protein destruction or turnover to maintain physiological homeostasis.Advances in quantitative transcriptomics,proteomics,and nuclease-based gene editing are now paving the global ways for exploring PTMs.In this review,we focus on recent developments in the PTM area and speculate on their importanee as a critical functional readout for the regulation of TME.A wealth of information has been emerging to prove useful in the search for conventional therapies and the development of global therapeutic strategies. | Wen Li Feifei Li Xia Zhang Huikuan Lin Chuan Xu | 2022 | Signal Transduction and Targeted Therapy2022，7，1： | 0 |
| 16 | 巴豆酰化效应蛋白在前列腺癌中的表达及其与肿瘤分期和分级的相关性显示文摘目的 探讨巴豆酰化效应蛋白ENL、AF9在前列腺癌中的表达水平以及与肿瘤分期分级和Gleason评分之间的相关性。方法 在GEPIA和Oncomine数据库中分析巴豆酰化效应蛋白ENL、AF9在前列腺癌中的表达情况;对包含不同分期分级的前列腺癌标本130例和正常前列腺组织31例的组织芯片进行免疫组织化学染色,检测ENL、AF9在各样本中的表达水平,并探究ENL、AF9的蛋白表达水平与前列腺癌分期分级和Gleason评分的关系。结果 与正常组织相比,ENL的mRNA表达水平和蛋白表达水平在前列腺癌中明显升高,且差异具有统计学意义。与正常组织相比,前列腺癌中AF9的mRNA表达水平和蛋白表达水平变化不明显,差异无统计学意义;ENL的蛋白表达水平与前列腺癌的分期和分级无明显相关性,与前列腺癌的Gleason评分呈正相关,且ENL的表达水平更高的前列腺癌患者的无疾病进展期生存期(Disease free survival, DFS)更短,差异具有统计学意义。结论 ENL在前列腺癌组织中显著高表达,与前列腺癌的恶性进展(Gleason评分)相关,可作为前列腺癌的诊断标志物之一,将来有可能作为前列腺癌的治疗靶点。 | 李帅 汪继洪 俞建军 | 2023 | 中国男科学杂志2023，37，6： | 0 |
| 17 | 新型组蛋白赖氨酸酰基化修饰调控的基因转录及疾病显示文摘染色质是人类遗传信息的载体,位于染色质上的基因在不同的时空条件下的精准表达调控与DNA的可接触性和染色质相关复合物的密切关联。组蛋白是染色质的重要组成成份,组蛋白上的多种化学修饰,例如乙酰化、甲基化和磷酸化等构成组蛋白密码,实时调控染色质的开放程度及转录调节复合物与染色质的结合,导致基因转录的激活或抑制。随着高分辨率质谱和专一性化学修饰抗体制备技术的提高,一系列新型组蛋白赖氨酸酰基化修饰,例如巴豆酰化、乳酸酰化和琥珀酰化等被发现,进一步扩展了组蛋白密码的多样性,显著增加了组蛋白密码调控基因转录的复杂性。本文着重概述了新近发现的赖氨酸巴豆酰化、乳酸酰化、琥珀酰化、异丁酰化、甲基丙烯酰化和异烟酰化等新型组蛋白赖氨酸酰基化修饰的书写、阅读及擦除的动态调控分子机制,总结了这些组蛋白酰基化修饰在基因表达中的功能及调控机制,阐述了新型组蛋白酰基化修饰与人类疾病的关联,提出新型组蛋白酰基化修饰研究面临的挑战和未来研究的方向。 | 齐吉红 张强 | 2023 | 中国生物化学与分子生物学报2023，39，10： | 0 |
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