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| 1 | RR-1 cuticular protein TcCPR69 is required for growth and metamorphosis in Tribolium castaneum显示文摘Cuticle is not only critical for protecting insects from noxious stimuli but is also involved in a variety of metabolic activities.Cuticular proteins(CPs)affect cuticle structure and mechanical properties during insect growth,reproduction,and environmental adaptation.Here,we describe the identification and characterization of a member of the RR-1 subfamily of CPs with an R&R consensus(CPR)in Tribolium castaneum(TcCPR69).Although it was previously reported to be highly expressed in the wings,we found that knocking down TcCPR69 by RNA interference(RNAi)did not cause obvious wing abnormalities but markedly disrupted the growth and metamorphosis of beetles with 100%cumulative mortality;additionally,the chitin content of the pharate adult was decreased and the new abdominal cuticle was significantly thinner before molting.TcCPR69 showed chitin-binding ability and the expression levels of key genes involved in chitin metabolism(trehalase[TcTRE],chitin synthase[TcCHSA and TcCHSB],and chitinase[TcCHT5 and TcCHT10])were also decreased by TcCPR69 knockdown.TcCPR69 gene expression peaked shortly after molting and was increased 2.61 fold at 12 h after 20-hydroxyecdysone(20E)injection.This was reversed by RNAi of the ecdysone-related genes ecdysone receptor(TcECR)and fushi tarazu transcription factor 1(TcFTZ-F1).These results indicate that TcCPR69 is positively regulated by 20E signaling to contribute to cuticle formation and maintain chitin accumulation during the growth and metamorphosis of beetles. | Jia Xie Guifang Peng Miao Wang Qisheng Zhong Xiaowen Song Jingxiu Bi Jing Tang Fan Feng Han Gao Bin Li | 2022 | Insect Science2022，29，6： | 0 |
| 2 | 飞蝗dpp基因在翅发育中的功能分析显示文摘Dpp(Decapentaplegic)是脊椎动物和无脊椎动物中非常重要且高度保守的一个信号通路,在细胞的生长和分化等方面发挥调控作用。在完全变态昆虫中,Dpp调控翅的生长发育,但对渐变态昆虫翅发育的作用机制尚不清楚。文章以飞蝗(Locusta migratoria)为研究对象,根据同源性搜索识别飞蝗dpp基因(Lmdpp),并对其编码的氨基酸序列与不同昆虫Dpp进行多序列比对。然后,利用RT-q PCR方法对Lmdpp在五龄飞蝗不同组织及不同发育天数的翅芽进行表达特性分析,结果显示Lmdpp在五龄每天的翅芽组织中均有表达,且在多个组织中高表达。利用RNA干扰技术探讨Lmdpp在飞蝗翅发育中的作用,结果显示,与对照组相比,注射Lmdpp基因的dsRNA(doublestranded RNA,dsRNA)的处理组约有31%的飞蝗在蜕皮后出现翅紊乱表型。苏木精-伊红(Hematoxylin-eosin,H&E)染色结果表明,沉默Lmdpp后的翅新表皮形成异常;RT-q PCR结果显示,沉默Lmdpp后导致飞蝗翅特异表皮蛋白基因LmAcp7、LmAcp8和LmAcp19(Adult cuticle protein,Acp)的表达量显著降低。结果表明,Lmdpp可能通过调控飞蝗翅特异表皮蛋白基因的表达参与翅的发育与形成,研究结果可为dpp在渐变态昆虫翅发育中的作用机制研究提供基础。 | 武兆辰 张福斌 徐子龙 张晶 张建珍 赵小明 | 2022 | 山西大学学报(自然科学版)2022，45，6： | 0 |
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