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| 1 | Present and future prospects for wheat improvement through genome editing and advanced technologies显示文摘Wheat(Triticum aestivum,2n=6x=42,AABBDD)is one of the most important staple food crops in the world.Despite the fact that wheat production has significantly increased over the past decades,future wheat production will face unprecedented challenges from global climate change,increasing world population,and water shortages in arid and semi-arid lands.Furthermore,excessive applications of diverse fertilizers and pesticides are exacerbating environmental pollution and ecological deterioration.To ensure global food and ecosystem security,it is essential to enhance the resilience of wheat production while minimizing environmental pollution through the use of cutting-edge technologies.However,the hexaploid genome and gene redundancy complicate advances in genetic research and precision gene modifications for wheat improvement,thus impeding the breeding of elite wheat cultivars.In this review,we first introduce state-of-the-art genome-editing technologies in crop plants,especially wheat,for both functional genomics and genetic improvement.We then outline applications of other technologies,such as GWAS,high-throughput genotyping and phenotyping,speed breeding,and synthetic biology,in wheat.Finally,we discuss existing challenges in wheat genome editing and future prospects for precision gene modifications using advanced genome-editing technologies.We conclude that the combination of genome editing and other molecular breeding strategies will greatly facilitate genetic improvement ofwheat for sustainable global production. | Shaoya Li Chen Zhang Jingying Li Lei Yan Ning Wang Lanqin Xia | 2021 | Plant Communications2021，2，4： | 4 |
| 2 | Recent advances in CRISPR/Cas9 and applications for wheat functional genomics and breeding显示文摘Common wheat(Triticum aestivum L.)is one of the three major food crops in the world;thus,wheat breeding programs are important for world food security.Characterizing the genes that control important agronomic traits and finding new ways to alter them are necessary to improve wheat breeding.Functional genomics and breeding in polyploid wheat has been greatly accelerated by the advent of several powerful tools,especially CRISPR/Cas9 genome editing technology,which allows multiplex genome engineering.Here,we describe the development of CRISPR/Cas9,which has revo-lutionized the field of genome editing.In addition,we emphasize technological breakthroughs(e.g.base editing and prime editing)based on CRISPR/Cas9.We also summarize recent applications and advances in the functional annotation and breeding of wheat,and we introduce the production of CRISPR-edited DNA-free wheat.Combined with other achievements,CRISPR and CRISPR-based genome editing will speed progress in wheat biology and promote sustainable agriculture. | Jun Li Yan Li Ligeng Ma | 2021 | aBIOTECH2021，2，4： | 3 |
| 3 | Gene editing applications to modulate crop flowering time and seed dormancy显示文摘Gene editing technologies such as CRISPR/Cas9 have been used to improve many agricultural traits,from disease resistance to grain quality.Now,emerging research has used CRISPR/Cas9 and other gene editing technologies to target plant reproduction,including major areas such as flowering time and seed dormancy.Traits related to these areas have important implications for agriculture,as manipulation of flowering time has multiple applications,including tailoring crops for regional adaptation and improving yield.Moreover,understanding seed dormancy will enable approaches to improve germination upon planting and prevent pre-harvest sprouting.Here,we summarize trends and recent advances in using gene editing to gain a better understanding of plant reproduction and apply the resulting information for crop improvement. | Olena Kishchenko Yuzhen Zhou Satyvaldy Jatayev Yuri Shavrukov Nikolai Borisjuk | 2020 | aBIOTECH2020，1，4： | 0 |
| 4 | 基因编辑技术及其在农作物中的应用进展显示文摘全球气候变化、人口增长、耕地减少和极端天气频发等为粮食安全和农业可持续发展带来诸多挑战。以CRISPR/Cas为代表的基因组编辑技术能够快速定向创制农作物新种质,提高育种效率,为保障粮食安全和生态安全提供有力的技术和材料支撑。综述了CRISPR/Cas系统介导的基因敲除、单碱基编辑、精准替换和引导编辑技术的研发及其在农作物遗传改良中的应用进展,并对我国农作物基因编辑研究进行了展望和建议,以期为今后农作物基因编辑研究的发展和应用提供参考。 | 闫磊 张金山 朱健康 夏兰琴 | 2022 | 中国农业科技导报2022，24，12： | 0 |
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