|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effect of human umbilical cord-derived mesenchymal stem cells on lung damage in severe COVID-19 patients:a randomized,double-blind,placebo-controlled phase 2 trial显示文摘Treatment of severe Coronavirus Disease 2019(COVID-19)is challenging.We performed a phase 2 trial to assess the efficacy andsafety of human umbilical cord-mesenchymal stem cells(UC-MScs)to treat severe coViD-19 patients with lung damage,based onour phase 1 data.In this randomized,double-blind,and placebo-controlled trial,we recruited 101 severe coVID-19 patients withlung damage.They were randomly assigned at a 2:1 ratio to receive either UC-MSCs(4×10^(7)cells per infusion)or placebo on day 0,3,and 6.The primary endpoint was an altered proportion of whole lung lesion volumes from baseline to day 28.Other imagingoutcomes,6-minute walk test(6-MWT),maximum vital capacity,diffusing capacity,and adverse events were recorded and analyzed.In all,100 COVID-19 patients were finally received either UC-MSCs in=65)or placebo(n=35).UC-MSCs administrationexerted numerical improvement in whole lung lesion volume from baseline to day 28 compared with the placebo(the mediandifference was-13.31%,95%Cl-29.14%,2.13%,P=0.08).UC-MSCs significanty reduced the proportions of solid componentlesion volume compared with the placebo(median difference:-15.45%;95%CI-30.82%,-0.39%;P=0.043).The 6-MWT showedan increased distance in patients treated with UC-MSCs(difference:27.00 m;95%CI 0.00,57.00;P=0.057).The incidence of adverseevents was similar in the two groups.These results suggest that UC-MSCs treatment is a safe and potentially effective therapeuticapproach for COVID-19 patients with lung damage.A phase 3 trial is required to evaluate effects on reducing mortality andpreventing long-term pulmonary disability. | Lei Shi Hai Huang Xuechun Lu Xiaoyan Yan Xiaojing Jiang Ruonan Xu Siyu Wang Chao Zhang Xin Yuan Zhe Xu Lei Huang Jun-Liang Fu Yuanyuan Li Yu Zhang Wei-Qi Yao Tianyi Liu Jinwen Song Liangliang Sun Fan Yang Xin Zhang Bo Zhang Ming Shi Fanping Meng Yanning Song Yongpei Yu Jiqiu Wen Qi Li Qing Mao Markus Maeurer Alimuddin Zumla Chen Yao Wei-Fen Xie Fu-Sheng Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 13 |
| 2 | 新型冠状病毒肺炎患者早期外周血淋巴细胞亚群及细胞因子特征显示文摘目的分析新型冠状病毒肺炎(COVID-19)早期(起病14天内)外周血免疫学特征及其与疾病严重程度的关系。方法收集2020年1月至3月广州医科大学附属市八医院收治的98例确诊COVID-19患者住院资料,分析COVID-19早期外周血淋巴细胞亚群[CD4^(+)T、CD8^(+)T细胞、B细胞、自然杀伤(NK)细胞、NKT细胞与调节性T细胞]和细胞因子[白细胞介素(IL)-2、IL-4、IL-6、IL-10、肿瘤坏死因子-α和干扰素-γ]变化特征,以及上述指标与患者病情严重程度的关系。年龄、起病时间、白细胞、中性粒细胞、淋巴细胞、淋巴细胞亚群和细胞因子水平为非正态分布的计量资料,3组患者间比较采用Kruskal-Wallis检验;性别、基础疾病、吸烟、武汉流行病学史、家庭成员被感染、症状、实验室指标异常比例为计数资料,采用Pearson卡方检验或Fisher确切概率检验;以Logistic回归分析影响疾病严重性的高危因素。结果根据病情严重程度将入组患者分为轻型组(17例)、普通型组(73例)和重型组(8例)。早期55.1%(54/98)患者CD4^(+)T细胞、40.8%(40/98)患者CD8^(+)T细胞和11.8%(10/85)患者B细胞计数下降。3组患者间CD4^(+)T细胞(P=0.010)、CD8^(+)T细胞(P=0.003)和B细胞(P=0.022)计数存在显著差异,以重型组水平最低。3组患者IL-6表达差异有统计学意义(P=0.001),以重型组水平最高,而其他细胞亚群及细胞因子水平则无显著差异(P均> 0.05)。Logistic回归分析显示,IL-6升高(OR=12.277、P=0.024)和年龄> 50岁(OR=21.823、P=0.030)分别为发生肺炎和重型化的独立预测因素。结论 COVID-19早期即明显破坏患者机体细胞和体液免疫应答,与疾病进展密切相关。 | 廖宝林 施海燕 刘艳霞 赵涵 焦倩 李丽雅 王信 曹意 何浩岚 | 2021 | 中华实验和临床感染病杂志(电子版)2021，15，3： | 8 |
| 3 | The signal pathways and treatment of cytokine storm in COVID-19显示文摘The Coronavirus Disease 2019(COVID-19)pandemic has become a global crisis and is more devastating than any other previous infectious disease.It has affected a significant proportion of the global population both physically and mentally,and destroyed businesses and societies.Current evidence suggested that immunopathology may be responsible for COVID-19 pathogenesis,including lymphopenia,neutrophilia,dysregulation of monocytes and macrophages,reduced or delayed type I interferon(IFN-I)response,antibody-dependent enhancement,and especially,cytokine storm(CS).The CS is characterized by hyperproduction of an array of pro-inflammatory cytokines and is closely associated with poor prognosis.These excessively secreted pro-inflammatory cytokines initiate different inflammatory signaling pathways via their receptors on immune and tissue cells,resulting in complicated medical symptoms including fever,capillary leak syndrome,disseminated intravascular coagulation,acute respiratory distress syndrome,and multiorgan failure,ultimately leading to death in the most severe cases.Therefore,it is clinically important to understand the initiation and signaling pathways of CS to develop more effective treatment strategies for COVID-19.Herein,we discuss the latest developments in the immunopathological characteristics of COVID-19 and focus on CS including the current research status of the different cytokines involved.We also discuss the induction,function,downstream signaling,and existing and potential interventions for targeting these cytokines or related signal pathways.We believe that a comprehensive understanding of CS in COVID-19 will help to develop better strategies to effectively control immunopathology in this disease and other infectious and inflammatory diseases. | Lan Yang Xueru Xie Zikun Tu Jinrong Fu Damo Xu Yufeng Zhou | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 7 |
| 4 | Pathological and molecular examinations of postmortem testis biopsies reveal SARS-CoV-2 infection in the testis and spermatogenesis damage in COVID-19 patients显示文摘In late December 2019,the novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which causes coronavirus disease 2019(COVID-19),was identified in Wuhan,China,and the ensuing pandemic has led to more than 50 million infected individuals and more than one million deaths by November 10,2020(https://covid19.who.int/).Pathologic investigations of autopsy tissue have focused primarily on the lung,heart,and kidney,whereas morphologic data on testis injury and the effects of SARS-CoV-2 infection on spermatogenesis are limited.Although two groups did not detect SARS-CoV-2 in the semen or testes of recovered COVID-19 patients,1,2 another group confirmed SARS-CoV-2 in the semen of patients.3 Therefore,it is currently unknown whether SARS-CoV-2 infection impacts spermatogenesis and male fertility.In the present study,we evaluated the effects of SARS-CoV-2 infection on spermatogenesis by examining the pathophysiology and molecular features of testes obtained from five male COVID-19 patients at autopsy. | Xixiang Ma Chuhuai Guan Rong Chen Yunyun Wang Shenglei Feng Rongshuai Wang Guoqiang Qu Sijia Zhao Fengli Wang Xiaoli Wang Dingyu Zhang Liang Liu Aihua Liao Shuiqiao Yuan | 2021 | Cellular & Molecular Immunology2021，18，2： | 6 |
| 5 | 新型冠状病毒肺炎的细胞因子风暴及其药物治疗显示文摘细胞因子风暴被认为是重型和危重型新型冠状病毒肺炎(COVID-19)患者死亡的重要原因,表现为过激的炎症反应:免疫细胞过度激活和细胞因子过度释放,造成肺毛细血管内皮细胞和肺泡上皮细胞弥漫性损伤,引发呼吸衰竭,在循环系统推动下细胞因子到达多个器官造成多器官衰竭。白细胞介素6拮抗剂、糖皮质激素和恢复者血清等是目前的免疫治疗药物。本综述旨在综述COVID-19疾病过程中细胞因子风暴发生的可能机制及抑制细胞因子风暴的潜在治疗药物。 | 杨艳 龚慧 赵翊畅 徐萍 张毕奎 颜苗 | 2020 | 中国医院用药评价与分析2020，20，5： | 5 |
| 6 | 新型冠状病毒肺炎患者实验室检查临床特点分析显示文摘目的:观察新型冠状病毒肺炎(coronavirus disease 2019,COVID-19)实验室检查指标与疾病严重程度的关系,并找出其发展成重型肺炎的危险因素。方法:纳入湖北省鄂州市第三人民医院综合楼隔离病房收治的COVID-19患者81例,其中普通型49例、重型32例,收集2组患者年龄、性别、慢性基础疾病、临床实验室检查指标进行统计对比分析,并使用受试者工作特征(receiver operating characteristic,ROC)曲线评估检查指标的预测价值。结果:81例COVID-19中,非重症组年龄(49.86±13.79)岁,重症组年龄(62.84±13.26)岁,重症组的年龄大于非重症组(P<0.05),合并有慢性基础疾病及白细胞计数(white blood cell count,WBC)、中性粒细胞计数(neutrophil count,NEU)、NEU百分比、C反应蛋白(C-reactive protein,CRP)、总胆红素(total bilirubin,TBIL)、肌酸激酶(creatine kinase,CK)、乳酸脱氢酶(lactate dehydrogenase,LDH)值越高的COVID-19患者越容易发展成重症肺炎(P<0.05);同时淋巴细胞计数(lymphocyte count,LYM)、LYM百分比、白蛋白值越低的COVID-19患者也容易发展成重症患者(P<0.05);对COVID-19患者重症风险的预测,患者年龄、WBC、NEU、NEU百分比、LYM、LYM百分比、ALB、CRP和LDH的ROC曲线下面积(area under the receiver-operating characteristic curve,AUC)分别为0.757、0.711、0.783、0.904、0.850、0.921、0.754、0.848和0.807。重复测量方差分析显示,NEU百分比、LYM百分比、CRP和LDH在不同病情组和治疗时间之间存在显著交互效应。结论:年龄大及合并慢性基础疾病的COVID-19患者更容易发展成重症,临床上可通过WBC、NEU、NEU百分比、LYM、LYM百分比、ALB、CRP和LDH评估COVID-19的严重程度,尤其是NEU百分比、LYM百分比结合患者年龄及肺部CT表现可预测其非重症患者进展成重症患者的危险性,以及时调整治疗方案。 | 汪晓凤 卢刚 | 2020 | 重庆医科大学学报2020，45，7： | 4 |
| 7 | 血管紧张素转化酶2在新型冠状病毒肺炎中的作用研究进展显示文摘新型冠状病毒肺炎(COVID-19)病原体被命名为SARS-CoV-2,与严重急性呼吸综合征冠状病毒(SARS-CoV)有较高的序列相似性。血管紧张素转化酶2(ACE2)被确认为SARS-CoV-2的受体,与SARS-CoV-2的感染和致病密切相关。靶向ACE2已成为目前最有希望攻克COVID-19的方向之一,研究ACE2在COVID-19中的作用,对治疗药物和疫苗的研发、遏制疫情扩散、减少并发症及降低病死率有重大意义。文章就ACE2的生理功能、ACE2在新型冠状病毒肺炎中的作用及靶向ACE2的防治策略进行综述,以期对COVID-19的再认识与药物研发有所帮助。 | 蔡金原(综述) 朱传龙(审校) | 2021 | 医学研究生学报2021，34，1： | 4 |
| 8 | COVID-19:immunopathogenesis and Immunotherapeutics显示文摘The recent novel coronavirus disease(COVID-19)outbreak,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is seeing a rapid increase in infected patients worldwide.The host immune response to SARS-CoV-2 appears to play a critical role in disease pathogenesis and clinical manifestations.SARS-CoV-2 not only activates antiviral immune responses,but can also cause uncontrolled inflammatory responses characterized by marked pro-inflammatory cytokine release in patients with severe COVID-19,leading to lymphopenia,lymphocyte dysfunction,and granulocyte and monocyte abnormalities.These SARS-CoV-2-induced immune abnormalities may lead to infections by microorganisms,septic shock,and severe multiple organ dysfunction.Therefore,mechanisms underlying immune abnormalities in patients with COVID-19 must be elucidated to guide clinical management of the disease.Moreover,rational management of the immune responses to SARSCoV-2,which includes enhancing anti-viral immunity while inhibiting systemic inflammation,may be key to successful treatment.In this review,we discuss the immunopathology of COVID-19,its potential mechanisms,and clinical implications to aid the development of new therapeutic strategies against COVID-19. | Li Yang Shasha Liu Jinyan Liu Zhixin Zhang Xiaochun Wan Bo Huang Youhai Chen Yi Zhang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 4 |
| 9 | 新型冠状病毒肺炎患者Mg^2+水平对CD^8+T淋巴细胞和NK细胞功能的影响显示文摘目的:探讨新型冠状病毒肺炎(COVID-19)患者血清及外周血单个核细胞(PBMCs)中Mg^2+水平变化及其对CD8^+T淋巴细胞和NK细胞功能的影响。方法:选取2020年1月20日~2月20日期间鄂州市中心医院收治的COVID-19确诊患者165例,其中轻型/普通型组98例,重型/危重型组67例,另选34例健康体检者作为对照组。采集外周血,分离PBMCs,检测各组血清及PBMCs中Mg^2+水平;流式细胞术检测各组外周血CD8^+T淋巴细胞和NK细胞亚群以及其表面抑制分子程序性死亡受体1(PD-1)和激活分子自然杀伤细胞活化受体(NKG2D)的表达水平;并分析Mg^2+水平与PD-1和NKG2D表达水平的相关性。结果:与对照组比较,轻型/普通型组和重型/危重型组患者血清及PBMCs中Mg^2+水平、CD8^+T淋巴细胞和NK细胞计数均明显降低(P<0.05),同时CD8^+T淋巴细胞和NK细胞表面抑制分子PD-1表达水平明显升高(P<0.05),而活化分子NKG2D表达水平均明显降低(P<0.05)。重型/危重型组患者以上各指标改变幅度又大于轻型/普通型组(P<0.05),且COVID-19患者Mg^2+水平与CD8^+T淋巴细胞和NK细胞表面分子PD-1表达水平呈负相关性(P<0.05),与NKG2D表达水平呈正相关性(P<0.05)。结论:COVID-19患者血清及PBMCs中Mg^2+水平会明显降低,其可能会导致CD8^+T淋巴细胞和NK细胞功能被抑制。 | 谢玲 程丰 龚国富 | 2021 | 海南医学院学报2021，27，1： | 3 |
| 10 | 新型冠状病毒感染消化系统的研究进展显示文摘由SARS-CoV-2引起的新型冠状病毒肺炎(coronavirus disease 2019,COVID-19)已导致全球大流行。截至目前,确诊感染人数已超过2945万,给全球多个国家人民的生活、工作和经济带来了沉重负担。随着病例数量的快速增长,大量证据表明,SARS-CoV-2不仅感染呼吸系统,还会影响消化系统,并且存在粪-口途径传播的可能性。本文通过回顾疫情爆发以来发表的文献并结合我们临床救治实践,将从消化系统表现、病理学与发病机制、粪便分析及消化内镜检查管理等方面来介绍新冠病毒肺炎与消化系统的关系。其目的是提高人们对新型冠状病毒肺炎的认识,并为该病的预防和治疗提供帮助。 | 林鹭 朱慧瑾 赵旺 赵思琪 李啸峰 | 2020 | 现代消化及介入诊疗2020，25，10： | 3 |
| 11 | NKG2A and COVID-19: another brick in the wall显示文摘Coronavirus disease 2019(COVID-19)is a viral infection caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2;formerly designated as 2019-nCoV),a novel betacoronavirus firstly identified during a burst of respiratory illness cases in Wuhan City,Hubei Province,China.1 Unfortunately,within a few weeks,the SARS-COV2 virus started to spread globally,producing a pandemic of an extremely spreadable and potentially fatal disease,becoming a cause of great concern for global public health.1 Despite the current estimates of COVID-19 case fatality rate suggest that this coronavirus is less deadly than other pathogens driving other large-scale outbreaks,such as SARS,Middle East respiratory syndrome,or Ebola,the main concern is that this infection is able to spread more easily than other diseases,including seasonal influenza. | Luca Antonioli Matteo Fornai Carolina Pellegrini Corrado Blandizzi | 2020 | Cellular & Molecular Immunology2020，17，6： | 3 |
| 12 | NK细胞免疫治疗防治COVID-19应用潜力的研究进展显示文摘新型冠状病毒肺炎(corona virus disease 2019,COVID-19)作为一种新发型急性呼吸道传染病,目前已对全球公共卫生安全构成严重威胁。严重急性呼吸综合征冠状病毒2(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)感染导致COVID-19重症患者淋巴细胞减少、淋巴细胞功能障碍并引起不可控的炎症反应。由于自然杀伤细胞(natural killer cell,NK细胞)在抗病毒和免疫调节中具有重要作用,以NK细胞为基础的免疫治疗可能是有效抗击COVID-19的治疗策略之一。现就已发表的SARS-CoV-2临床研究报告,整理介绍了该病毒的基本特征及COVID-19患者的主要免疫病理学状态,阐述NK细胞在抗病毒领域的潜在作用机制与研究进展,并对NK细胞防治COVID-19的免疫治疗策略及所面临的问题与挑战作一综述。 | 侯晓军(综述) 杨敏(审校) | 2021 | 微生物学免疫学进展2021，49，1： | 3 |
| 13 | 心肌损伤对新型冠状病毒肺炎危重型患者预后的影响及其原因分析显示文摘目的:探讨心肌损伤对新型冠状病毒肺炎(COVID-19)危重型患者预后的影响并分析其原因。方法:回顾性分析2787例COVID-19重症、危重症患者的临床资料。结果:2787例患者中,危重型患者392例,其中死亡236例(8.47%)。死亡患者中,78.81%的患者在入院时心肌损伤标志物血清心肌高敏肌钙蛋白(hs-cTnI)水平升高。根据入院时血清hs-cTnI水平分为hs-cTnI升高组(>34.2 pg/mL)210例,hs-cTnI正常组(≤34.2 pg/mL)182例。血清hs-cTnI升高组患者机械通气治疗的构成比更高(92.86%vs 64.29%,P<0.01)。血清hs-cTnI升高组14 d和30 d院内病死率明显高于正常组(65.71%vs 26.37%;84.28%vs 32.42%,均P<0.01)。患者血清hs-cTnI升高与14 d和30 d院内病死率相关(OR=2.34、3.15)。与hs-cTnI正常组比较,血清hs-cTnI升高组外周血淋巴细胞计数更低,而血清降钙素原、C-反应蛋白、白介素-2R、白介素-6、白介素-8、白介素-10及肿瘤坏死因子-α水平更高,提示病毒引发的炎症因子风暴可能是导致心肌损伤的原因。结论:心肌损伤是COVID-19危重型患者院内死亡的重要危险因素,其潜在机制可能是新型冠状病毒感染诱发的细胞因子风暴介导的炎症损伤。 | 汪璐芸 陈鹏 余波 李陈泽 周宁 汪道文 | 2020 | 内科急危重症杂志2020，26，6： | 2 |
| 14 | 从免疫系统相关发病机制探讨COVID-19防治策略显示文摘新型冠状病毒肺炎(Corona Virus Disease 2019,COVID-19)自暴发以来,已造成全球2.1%的人口感染和数百万人死亡,成为当前全世界重要的公共卫生问题。免疫系统对控制和清除新型冠状病毒(Severe Acute Respiratory Syndrome Coronavirus 2,SARS-CoV-2)至关重要,免疫系统反应失调可加重肺组织炎性损伤及其他器官损害。本文将从免疫机制的角度,就SARS-CoV-2的感染过程、宿主针对其产生的固有免疫及适应性免疫应答、SARS-CoV-2疫苗的分类和制备及免疫治疗药物进行详细阐述,为COVID-19的诊断分型、病情监控及治疗预后提供科学依据。 | 孙之冰 许雅婷 施冬艳 陈云 | 2021 | 生物医学转化2021，2，2： | 2 |
| 15 | 间充质干细胞治疗新冠肺炎细胞因子风暴的临床研究进展显示文摘新冠病毒感染后,免疫系统被过度激活时会生成大量的炎症因子,导致严重的细胞因子风暴。细胞因子风暴可导致肺及多器官损伤,继而出现肺水肿、换气功能障碍、急性肺损伤、急性呼吸窘迫综合征、急性心脏损伤和继发感染,最终导致患者死亡。间充质干细胞(MSCs)是一类多潜能组织干细胞,在抗炎、再生、免疫调节等方面有着巨大潜力,能够通过直接接触或旁分泌多种细胞因子的方式调节免疫,适时抑制过激免疫反应,抑制细胞因子风暴,同时具有促进损伤组织恢复的能力。MSCs静脉输注在治疗急性呼吸窘迫综合征、急性肺损伤、重症肺炎上已被证实具有良好的安全性和有效性,在治疗新型冠状肺炎的临床试验也已经广泛开展。本文就MSCs用于控制新冠肺炎细胞因子风暴的情况作出综述,提供进一步临床研究参考信息。 | 陈娜 陈文燕 崔恩海 | 2022 | 临床肺科杂志2022，27，3： | 2 |
| 16 | 新型冠状病毒肺炎常见实验室指标与病情严重程度相关性的回顾性分析显示文摘目的分析新型冠状病毒肺炎(COVID-19)常见实验室指标与病情严重程度的相关关系。方法选择2020年1-2月在该院住院治疗的30例COVID-19患者,分为轻型组(14例,轻型和普通型)、重型组(16例,重型和危重型),同期收住院非COVID-19患者为普通肺炎组(48例)。分别检测各组天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、清蛋白(Alb)、总胆红素(TBiL)、结合胆红素(DBiL)、血尿素氮(BUN)、肌酐(CREA)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、白细胞计数(WBC)、中性粒细胞计数(NEU)、中性粒细胞百分比(NEU%)、淋巴细胞计数(LYMPH)、淋巴细胞百分比(LYMPH%)、单核细胞计数(MON)、单核细胞百分比(MON%)、C反应蛋白(CRP)、降钙素原(PCT)。采用SPSS22.0统计分析所有数据。结果重型组患者LDH、NEU、NEU%、CRP水平均高于轻型组、普通肺炎组(P<0.05),而Alb、LYMPH、LYMPH%、MON%均低于轻型组、普通肺炎组(P<0.05);与轻型组比较,普通肺炎组、重型组PCT水平均升高(P<0.05),但普通肺炎组与重型组之间比较,差异无统计意义(P>0.05);重型组患者随着病情加重WBC增加,MON逐渐减少,但与普通肺炎组、轻型组比较差异无统计学意义(P>0.05)。结论COVID-19患者轻型组与重型组常见实验室检测指标间存在明显差异,联合检测LDH、Alb、WBC、NEU%、LYMPH%、MON%、CRP、PCT等指标,对评估病情进展及严重程度有一定临床意义。 | 陆光辉 褚志华 田娅玲 周发为 王姣平 曾守逵 柳青子 | 2021 | 检验医学与临床2021，18，13： | 2 |
| 17 | Low serum neutralizing anti-SARS-CoV-2 S antibody levels in mildly affected COVID-19 convalescent patients revealed by two different detection methods显示文摘Neutralizing antibodies targeting the receptor-binding domain(RBD)of the SARS-CoV-2 spike(S)block severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)entry into cells via surface-expressed angiotensin-converting enzyme 2(ACE2).We used a surrogate virus neutralization test(sVNT)and SARS-CoV-2 S protein-pseudotyped vesicular stomatitis virus(VSV)vector-based neutralization assay(pVNT)to assess the degree to which serum antibodies from coronavirus disease 2019(COVID-19)convalescent patients interfere with the binding of SARS-CoV-2 S to ACE2.Both tests revealed neutralizing anti-SARS-CoV-2 S antibodies in the sera of ~90% of mildly and 100% of severely affected COVID-19 convalescent patients.Importantly,sVNT and pVNT results correlated strongly with each other and to the levels of anti-SARS-CoV-2 S1 IgG and IgA antibodies.Moreover,levels of neutralizing antibodies correlated with the duration and severity of clinical symptoms but not with patient age.Compared to pVNT,sVNT is less sophisticated and does not require any biosafety labs.Since this assay is also much faster and cheaper,sVNT will not only be important for evaluating the prevalence of neutralizing antibodies in a population but also for identifying promising plasma donors for successful passive antibody therapy. | Berislav Bosnjak Saskia Catherina Stein Stefanie Willenzon Anne Katrin Cordes Wolfram Puppe Gunter Bernhardt Inga Ravens Christiane Ritter Christian R.Schultze-Florey Nina Godecke Jorg Martens Hannah Kleine-Weber Markus Hoffmann Anne Cossmann Mustafa Yilmaz Isabelle Pink Marius M.Hoeper Georg M.N.Behrens Stefan Pohlm ann Rainer Blasczyk Thomas F.Schulz Reinhold Forster | 2021 | Cellular & Molecular Immunology2021，18，4： | 2 |
| 18 | 恶性肿瘤患者接种新型冠状病毒疫苗的建议及依据显示文摘新型冠状病毒(SARS-CoV-2)全球大流行已经影响到全世界超过2.18亿人,目前新型冠状病毒肺炎(COVID-19)尚缺乏标准的治疗方案,疫苗的研发与应用将有助于控制COVID-19大流行的状况。当前,世界各国均呼吁加快疫苗接种速度以达到最佳效果的群体免疫。但是,作为COVID-19感染的高危群体,肿瘤患者接种SARS-CoV-2疫苗的安全性及有效性尚缺乏可靠证据。鉴于此,本文将结合SARS-CoV-2的生物学特征、机体的免疫应答反应、疫苗的作用机制及对肿瘤患者的潜在影响几个方面,针对恶性肿瘤患者这一特殊人群能否接种SARS-CoV-2疫苗的相关建议进行总结,以期为恶性肿瘤患者接种疫苗的潜在可能性及风险提供科学的参考依据。 | 吴姝涵 李建辉 曹伟 姜楠 张菲菲 蔺光帅 白璐 杜嘉 祝旭龙 | 2022 | 空军军医大学学报2022，43，6： | 1 |
| 19 | Unique immunological profile in patients with COVID-19显示文摘The relationship between severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)and host immunity is poorly understood.We performed an extensive analysis of immune responses in 32 patients with severe COVID-19,some of whom succumbed A control population of healthy subjects was included.Patients with COVID-19 had an altered distribution of peripheral blood lymphocytes,with an increased proportion of mature natural killer(NK)cells and low T-cell numbers.NK cells and CD8^(+)T cells overexpressed T-cellimmunoglobulin and mucin domain-3(TIM-3)and CD69.NK cell exhaustion was attested by increased frequencies of programmed cell death protein 1(PD-1)positive cells and reduced frequencies of natural killer group 2 member D(NKG2D)-,DNAX accessory molecule-1(DNAM-1)-and sialic acid-binding Ig-like lectin 7(Siglec-7)-expressing NK cells,associated with a reduced ability to secrete interferon(IFN)γ.Patients with poor outcome showed a contraction of immature CD56^(bright) and an expansion of mature CD57^(+)F_(CEI)Rlγ^(eng) adaptive NK cells compared to survivors.Increased serum levels of IL-6 were also more frequently identified in deceased patients compared to survivors.Of note,monocytes secreted abundant quantities of IL-6,IL-8,and IL-1β which persisted at lower levels several weeks after recovery with concomitant normalization of CD69,PD-1 and TIM-3 expression and restoration of CD8^(+)T cell numbers.A hyperactivated/exhausted immune response dominate in severe SARS-CoV-2 infection,probably driven by an uncontrolled secretion of inflammatory cytokines by monocytes.These findings unveil a unique immunological profile in COVID-19 patients that will help to design effective stage-specific treatments for this potentially deadly disease. | Stefania Varchetta Dalila Mele Barbara Olivier Stefania Mantovani Serena Ludovisi Antonella Cerino Raffaele Bruno Alberto Castelli Mario Mosconi Marco Vecchia Silvia Roda Michele Sachs Catherine Klersy Mario U.Mondelli | 2021 | Cellular & Molecular Immunology2021，18，3： | 1 |
| 20 | 轻重症及转归阶段的COVID-19患者免疫细胞的单细胞转录谱分析显示文摘目的构建轻重症及转归阶段的新型冠状病毒肺炎(corona virus disease 2019,COVID-19)患者和健康对照的外周血单个核细胞(peripheral blood mononuclear cell,PBMC)的单细胞转录图谱,分析疾病进展及恢复与宿主免疫反应之间的关系。方法从公共数据库中获取健康对照和COVID-19重症、轻症、恢复早期、恢复晚期患者PBMCs的单细胞转录组(single-cell RNA sequence,scRNA-seq)测序数据,根据每个细胞的表达谱将细胞进行聚类后,根据已知的细胞标志物确定每个聚类的细胞亚型,统计并比较每个细胞亚型的比例,并对轻重症及转归阶段细胞的差异表达基因进行分析。结果控制数据质量后得到的115334个PBMCs分成22个细胞亚群。CD14+单核细胞、炎症性单核细胞、na?ve B细胞和中间记忆B细胞在重症患者PBMCs中明显升高;增殖性CD8+T/NK细胞在重症患者T细胞中的比例明显升高;XCL1+NK细胞在重症患者PBMCs中明显降低;cDC2细胞、pDC细胞在恢复期患者PBMCs中明显升高。随着疾病的缓解与康复,PBMCs中NK细胞的比例逐步升高。疾病期与康复期的PBMCs中与白细胞活化、白细胞黏附相关的基因均有上调。结论轻重症及转归阶段的COVID-19患者PBMCs在细胞比例和表达谱上变化较大。 | 王正 张倩月 宋怀东 | 2022 | 复旦学报(医学版)2022，49，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员